期刊论文

中文

中国动物传染病学报

1674-6422

2014

3

19-25

:黎摄儿,男，硕士研究生，预防兽医专业

本校为第一机构

参照GenBank中羊传染性脓疱病毒（Orf virus,ORFV）的毒力因子趋化因子结合蛋白（chemokine-binding protein,CBP）基因的核苷酸序列设计合成1对特异性引物,以羊传染性脓疱病毒湖北分离毒株的DNA为模板,提取总DNA。采用PCR方法特异扩增该基因片段,得到CBP基因序列。通过网络平台的SOPMA服务器和DNAStar软件预测ORFV CBP蛋白的二级结构;综合利用DNAStar软件、二级结构预测及ABCpred方案预测羊ORFV CBP蛋白的B细胞表位;分别采用人工神经网络法（ANNA）和在线程序预测ORFV CBP蛋白的细胞毒性T细胞和辅助T细胞的抗原表位。结果显示,ORFV CBP蛋白的二级结构主要包括α-螺旋、β-转角、无规则卷曲和β...

According to the published chemokine-binding protein （CBP） gene sequence of Orf virus in the GenBank, a pair of primers were designed and synthesized for amplification in PCR using total DNA of Orf virus Hubei strain as template. The PCR products of the CBP gene were sequenced. Subsequently, the secondary structure of the CBP protein was predicted using the network platform SOPMA server and DNAStar software. The B cell epitopes of the CBP protein were predicted by comprehensive utilization of the DNAStar software, the secondary structure pred...