期刊论文

中文

中国动物传染病学报

1674-6422

2018

26

2

80-84

国家科技支撑计划项目(2015BAD11B00) 国家自然科学基金项目(C180701)。

本校为第一机构

为研究抗球虫新化合物沙咪珠利在鸡盲肠中的药物代谢和消除情况,建立检测鸡盲肠中沙咪珠利的高效液相色谱法.检测方法以妥曲珠利亚砜为内标,X TERRA MS C185μm(Waters,4.6×250 mm)柱为分析柱,乙腈:0.2%磷酸水溶液(v/v,35:65)为流动相等度洗脱,C18柱分离后,在251 nm波长紫外检测器检测,内标法定量.鸡盲肠样品经匀浆化后,乙腈提取、己烷除脂.结果表明,该方法在0.2~40 mg/kg添加浓度范围内检测鸡盲肠中沙咪珠利具有很好的线性关系(r2=0.994);平均相对回收率为90%~107%,日内变异系数2.37%~8.72%;日间变异系数4.09%~6.80%,具有特异性好、灵敏度高、准确性和重现性良好、操作简单等优点,能满足鸡盲肠...

The metabolism and elimination of a new anticoccidial drug ethanamizuril in chicken caecum was determined using high performance liquid chromatography. Separation was carried out on a X TERRA MS C18 column (Waters, 4.6×250 mm) with acetonitrile-0.2% phosphoric acid aqueous solution (v:v, 35:65) as a mobile phase. PDA UV detection was done at 251 nm. Toltrazuril sulfoxide was used as internal standard. Caecum samples of chickens were homogenized and ethanamizuril was extracted with acetonitrile followed by defatting with hexane. The detection l...