Background-Free Imaging of a Viral Capsid Proteins Coated Anisotropic Nanoparticle on a Living Cell Membrane with Dark-Field Optical Microscopy

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成果类型：

期刊论文

作者：

Ye, Zhongju;Wei, Lin;Zeng, Xuyao;Weng, Rui;Shi, Xingbo;...

通讯作者：

Xiao, Lehui

作者机构：

[Chen, Langxing; Ye, Zhongju; Xiao, Lehui; Zeng, Xuyao] Nankai Univ, Coll Chem, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China.

[Wei, Lin; Xiao, Lehui] Hunan Normal Univ, Key Lab Chem Biol & Tradit Chinese Med Res, Key Lab Phytochem R&D Hunan Prov, Minist Educ,Coll Chem & Chem Engn, Changsha 410081, Hunan, Peoples R China.

[Weng, Rui] Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Minist Agr, Key Lab Agrofood Safety & Qual, Beijing 100081, Peoples R China.

[Shi, Xingbo] Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

[Wang, Naidong] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China.

通讯机构：

[Xiao, Lehui] N

[Xiao, Lehui] H

Nankai Univ, Coll Chem, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China.

Hunan Normal Univ, Key Lab Chem Biol & Tradit Chinese Med Res, Key Lab Phytochem R&D Hunan Prov, Minist Educ,Coll Chem & Chem Engn, Changsha 410081, Hunan, Peoples R China.

语种：

英文

期刊：

Analytical Chemistry

ISSN：

0003-2700

年：

2018

卷：

期：

页码：

1177-1185

DOI：

10.1021/acs.analchem.7b03762

基金类别：

national natural science foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [21405045, 21522502]

机构署名：

本校为其他机构

院系归属：

动物医学院

食品科学技术学院

摘要：

Exploring the diffusion dynamics of a viral capsid proteins (VCP)-functionalized nanocarrier on a living cell membrane could provide much kinetic information for the better understanding of their biological functionality. Gold nanoparticles are an excellent core material of nanocarriers because of the good biocompatibility as well as versatile surface chemistry. However, due to the strong scattering background from subcellular organelles, it is a grand challenge to selectively image an individual nanocarrier on a living cell membrane. In this w...

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Background-Free Imaging of a Viral Capsid Proteins Coated Anisotropic Nanoparticle on a Living Cell Membrane with Dark-Field Optical Microscopy

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