National Key Research and Development Project#&#ߢYFD0100404
the National Program of Transgenic Variety Development of China#&#ߙZX08005-012B
Science and Technology Innovation Project of Hunan Province#&#ߢJJ2014
Key Research and Development Project of Hunan Province#&#ߤNK2023
机构署名：
本校为第一且通讯机构
院系归属：
农学院
摘要：
AbstractMost of the cotton bollworm-resistant genes applied in cotton are more than 20years and they all belong to Cry1Ab/c family, but the insect-resistant effects of Cry5Aa on cotton were rarely reported. The possible risk of resistance is increasing. The study synthesized a novel bollworm-resistant gene Cry5Aa artificially based on preferences of cotton codon. The new gene was transferred to cotton through the method of pollen tube pathway. The transgenic strains were identified by kanamycin test in field and laboratory PCR analysis. Meanwhile, an insect resistance test was conducted by art...
