Rice black-streaked dwarf virus genome segment S5 is a bicistronic mRNA in infected plants

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成果类型：

期刊论文

作者：

Yang, Jian;Zhang, Heng-Mu*;Ying, Lu;Li, Jing;Lv, Ming-Fang;...

通讯作者：

Zhang, Heng-Mu

作者机构：

[Yang, Jian; Liang-Ying, Dai] Hunan Agr Univ, Coll Biosafety Sci & Technol, Changsha 410128, Hunan, Peoples R China.

[Xie, Li; Yang, Jian; Liu, Xiao-Ya; Li, Jing; Zhang, Heng-Mu; Li, Pei-Pei; Ying, Lu; Chen, Jian-Ping; Lv, Ming-Fang] Zhejiang Acad Agr Sci, Inst Virol & Biotechnol,Minist Agr, Zhejiang Prov Key Lab Plant Virol,Key Lab Plant P, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China.

[Lv, Ming-Fang] Zhejiang Univ, CAB, Hangzhou 310058, Zhejiang, Peoples R China.

[Liu, Xiao-Ya; Li, Pei-Pei] Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China.

通讯机构：

[Zhang, Heng-Mu] Z

Zhejiang Acad Agr Sci, Inst Virol & Biotechnol,Minist Agr, Zhejiang Prov Key Lab Plant Virol,Key Lab Plant P, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China.

语种：

英文

关键词：

Internal Ribosome Entry Site;Dwarf Virus;Internal Ribosome Entry Site Element;Internal Ribosome Entry Site Activity;Northern Blot Assay

期刊：

Archives of Virology

ISSN：

0304-8608

年：

2014

卷：

159

期：

页码：

307-314

DOI：

10.1007/s00705-013-1832-2

基金类别：

This work was funded by the National Science and Technology Support Program (2012BAD19B03), the China 973 Program (2010CB126203), the Special Fund for Agro-Scientific Research in the Public Interest of China (201003031), the 863 program (2007AA10Z414), the Zhejiang Provincial Science and Technology Project (2010C12027), and the Zhejiang Provincial Foundation for Natural Science (Z305165 and Y3090657). We thank Professor M. J. Adams, Rothamsted Research, Harpenden, UK, for help in correcting the English of the manuscript.

机构署名：

本校为第一机构

院系归属：

植物保护学院

摘要：

Rice black-streaked dwarf virus (RBSDV) is a recognized member of the genus Fijivirus, family Reoviridae. Genome segment S5 has a putative second ORF partially overlapping the major ORF but in a different reading frame. This putative ORF is present in a published sequence and in two Chinese isolates now sequenced. Antibodies were raised against purified P5-1 and P5-2 fusion proteins expressed in a prokaryotic system. In western blots, these antibodies reacted with proteins of about 106 and 27 kDa, respectively, as predicted by sequence analysis. In immunoelectron microscopy, antibodies to P5-1...

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Rice black-streaked dwarf virus genome segment S5 is a bicistronic mRNA in infected plants

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