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Development of a Sensitive Escherichia coli Bioreporter Without Antibiotic Markers for Detecting Bioavailable Copper in Water Environments

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成果类型:
期刊论文
作者:
Pang, Yilin;Ren, Xiaojun;Li, Jianghui;Liang, Feng;Rao, Xiaoyu;...
通讯作者:
Lyu, Jianxin;Tan, Guoqiang
作者机构:
[Ren, Xiaojun; Zhou, Huaibin; Liang, Feng; Lyu, Jianxin; Gao, Yang; Pang, Yilin; Rao, Xiaoyu; Wu, Wenhe] Wenzhou Med Univ, Zhejiang Prov Key Lab Med Genet, Key Lab Lab Med, Minist Educ,Sch Lab Med & Life Sci, Wenzhou, Peoples R China.
[Pang, Yilin] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Peoples R China.
[Jiang, Fengying; Wang, Wu; Wang, Juanjuan; Tan, Guoqiang; Zhao, Jianguo; Li, Jianghui; Li, Dong; Hong, Xufen] Wenzhou Med Univ, Sch Lab Med & Life Sci, Zhejiang Prov Key Lab Technol & Applicat Model Or, Key Lab Lab Med,Minist Educ,Lab Mol Med, Wenzhou, Peoples R China.
[Lyu, Jianxin] Hangzhou Med Coll, Peoples Hosp, Hangzhou, Peoples R China.
通讯机构:
[Lyu, Jianxin; Tan, Guoqiang] W
[Lyu, Jianxin] H
Wenzhou Med Univ, Zhejiang Prov Key Lab Med Genet, Key Lab Lab Med, Minist Educ,Sch Lab Med & Life Sci, Wenzhou, Peoples R China.
Wenzhou Med Univ, Sch Lab Med & Life Sci, Zhejiang Prov Key Lab Technol & Applicat Model Or, Key Lab Lab Med,Minist Educ,Lab Mol Med, Wenzhou, Peoples R China.
Hangzhou Med Coll, Peoples Hosp, Hangzhou, Peoples R China.
语种:
英文
关键词:
Whole-cell bioreporter;Detecting bioavailable copper;Gene knock-in;GFPmut2;Escherichia coli
期刊:
Frontiers in Microbiology
ISSN:
1664-302X
年:
2020
卷:
10
页码:
485312
基金类别:
We would like to thank Dr. Huangen Ding (Louisiana State University, United States) for kindly providing the suggestive advices for this manuscript. We would also like to thank Dr. Lirui Liu (The University of Hong Kong) for English language editing. Funding. This study was supported by the Chinese National Natural Science Foundation Grants (81671124 and 31870775), the National High Technology Research and Development Program of China (863 Program) (2014AA06A514), and Key Discipline of Zhejiang Province in Medical Technology (first class, category A).
机构署名:
本校为其他机构
院系归属:
生物科学技术学院
摘要:
The whole-cell bioreporters based on the cop-operon sensing elements have been proven specifically useful in the assessment of bioavailable copper ions in water environments. In this study, a series of experiments was conducted to further improve the sensitivity and robustness of bioreporters. First, an Escherichia coli △copA△cueO△cusA mutant with three copper transport genes knocked out was constructed. Then, the copAp::gfpmut2 sensing element was inserted into the chromosome of E. coli △copA△cueO△cusA by gene knock-in method to obtain t...

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