期刊论文

中文

西北农业学报

1004-1389

2007

16

5

92-95,104

10.3969/j.issn.1004-1389.2007.05.022

2002AA207009:国家高技术研究发展计划（863计划） 30671206:国家自然科学基金 2006FJ4265:湖南省博士后专项基金

本校为第一机构

采用砂培试验,在正常供氮条件下测定油菜花后不同时期叶片蛋白水解酶、谷氨酰胺合成酶(glutamine synthetase,GS)和籽粒谷氨酸合酶(glutamate:z-oxoglutarate aminotransferase,即glutamate synthase,GOGAT)活性以及韧皮部汁液游离氨基酸和L-谷氨酰胺含量.结果表明,随着花后植物体的衰老,叶片蛋白水解酶活性虽有所波动但总体上呈下降趋势;叶片GS活性在角果发育初期和中后期维持较高水平,此后均有下降,有效地保证了籽粒发育初期酶蛋白形成的需要及籽粒发育后期氮素再分配的顺利进行;随着籽粒的发育进程,籽粒GOGAT活性逐渐升高,到角果发育后期达最高值,此后又下降.比较两个油菜品种以上3种酶的活性...

By sandy cultivation and with normal nitrogen supply, this experiment determined the active of the proteolytic enzymes and the glutamine synthetase （GS） in blades and the active of glutamate zoxoglutarate aminotransferase （GOGAT） in pods at different time during the later growing period, as well as determining the content of dissociated amino acid and L-Glutamine in the juice of phloem. The results suggested that with senescence of the plant during the period after blooming, generally the proteolytic enzymes active in blades declined, thoug...