期刊论文

中文

西北农业学报

1004-1389

2008

17

3

298-301,307

10.3969/j.issn.1004-1389.2008.03.065

本校为第一机构

以食用仙人掌嫩茎切块为外植体进行组织培养,研究了6-BA、 IAA和IBA对其组培快繁的影响.结果表明,诱导萌芽以在MS+6-BA1.0mg/L+IAA0.3mg/L培养基上效果最好,萌芽率达88.9%;最好的增殖培养基是MS+6-BA1.0mg/L+IAA0.3mg/L,增殖率为345.4%,且中下部分比上部增殖效果要好;在1/2MS+IBA0.5mg/L生根培养基上,平均每个苗长2.67个根,且有的根长达5cm.当苗高3cm,根长2cm时,炼苗3d后移栽于椰子壳:珍珠岩为1:1的混合基质中,成活率为95%.

Using the tender stems of Edible cactus as the explants,the effects of different medium on plant regeneration were examined.The results indicated that the appropriate medium for bud induction was MS＋6-BA1.0 mg/L＋IAA0.3 mg/L.the highest rate of bud induction was 88.9%.the medium of multiplication was MS＋6-BA1.0 mg/L＋IAA0.3 mg/L,the multiplication rate was 345.4%.the multiplication of the lower parts of the micro-cladode is higher.the medium of rooting was 1/2MS＋IBA0.5 mg/L,averagely every stem has 2.67 roots,some roots length was 5cm.When r...