期刊论文

Zhi Liu<&wdkj&>Junhua Chen

英文

Talanta

0039-9140

2023

252

123832

10.1016/j.talanta.2022.123832

CRedit author statement Fang Deng: Conceptualization, Methodology, Data curation, Formal analysis, Writing-original draft. Jiafeng Pan: Conceptualization, Methodology, Validation, Writing-original draft. Zhi Liu: Conceptualization, Methodology, Validation. Junhua Chen: Conceptualization, acquisition, Project administration, Writing-review & editing.

本校为第一机构

We successfully constructed several cascaded molecular logic gates (2INHIBIT-2AND, 2AND-2OR, and 2OR-2INHIBIT) using three different antibiotics as the inputs. In the presence of kanamycin (KAN), chloramphenicol (CHL), or oxytetracycline (OXY), the aptamer-antibiotic recognition will release the trigger DNA to active the hairpin DNA hybridization. Exonuclease III (Exo III)-mediated catalysis reaction was introduced in the logic system to generate Mg2+-dependent DNAzyme, which was used to cleave the fluorescence signal reporter probe. For input, the presence and absence of the antibiotic was de...