期刊论文

中文

毒理学杂志

1002-3127

2011

25

2

79-83

10.16421/j.cnki.1002-3127.2011.02.007

国家科技重大专项“重大新药创制”(2009ZX09501-034)；

本校为第一机构

目的通过氯丙嗪染毒体外培养的大鼠卵巢颗粒细胞,研究其对卵巢颗粒细胞的毒性作用,并初步探讨可能的作用机制。方法对未成熟的Wistar大鼠卵巢颗粒细胞进行原代培养,用不同浓度的氯丙嗪(0、0.1、1和10μmol/L)染毒24 h。MTT法检测细胞相对活力,ELISA法检测培养液中孕酮(P)和雌二醇(E2)的含量,Hoechst 33258检测颗粒细胞的凋亡变化。结果在本次实验所设置的剂量范围内,与对照组比较,氯丙嗪能明显地抑制大鼠卵巢颗粒细胞增殖和E2的分泌(P

Objective To study the toxic effects and explore the possible mechanism of Chlorpromazine exposure to rat ovarian granulosa cells in vitro.Methods Immature primary cultured ovarian granulosa cells of wister rat were treated with various concentrations of chlorpromazine（0,0.1,1,10 μmol/L） for 24 h.After 24-h exposure to chlorpromazine,cell viability was detected by MTT assay,content of progesterone（P） and estradiol（E2） collected from medium were detected by ELISA assay.And ovarian granulosa cells stained with Hochst dye 33258 were checked...