期刊论文

Xie, Bingyan

英文

Applied Microbiology and Biotechnology

0175-7598

2019

103

15

6187-6194

10.1007/s00253-019-09945-2

Funding information This work was supported by the National Key R&D Program of China 2017YFD0200600 to Z.M., 2018YFD0201200 to Y.L., Central Public-Interest Scientific Institution Basal Research Fund Y2017JC28 to B.X., National Natural Science Foundation of China (NSFC) grants 31772214 to Y.Y., China Agriculture Research System, CARS-25, and Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS).

本校为其他机构

Purpureocillium lilacinum is a promising commercial agent for controlling plant-parasitic nematodes and plant pathogens. Leucinostatins are a family of lipopeptides produced by P. lilacinum that are synthesized, modified, and regulated by a gene cluster consisting of 20 genes. Sequence analyses have indicated that lcsL, a gene in the lcs cluster, is a putative bZIP transcription factor. In this study, the CRISPR-Cas9 system was introduced to increase the efficiency of homologous recombination for the disruption of lcsL. The expression of genes in the cluster was significantly reduced in lcsL d...