Based on uniform design and support vector regression, UD-SVR, a novel experimental design and analysis method for the prescription optimization was proposed. It was applied to optimize the complicated fermentation medium including nine factors for a variant of Escherichia coli. The variant could produce glutamate decarboxylase which transformed gultamic acid into gamma aminobutyric acid in vitro. The optimization results of the medium by UD-SVR showed that OD630, an activity index of glutamate decarboxylase, increased from 1.528 in the initial median to 2.303 in the optimal medium after testi...
