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Comprehensive Analysis of the Transcriptome-Wide m6A Methylome in Shaziling Pig Testicular Development

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成果类型:
期刊论文
作者:
Chen, Chujie;Tang, Xiangwei;Yan, Saina;Yang, Anqi;Xiang, Jiaojiao;...
通讯作者:
Chen, Bin;Gu, JJ
作者机构:
[Chen, Chujie; Tang, Xiangwei; Yan, Saina; Gu, JJ; Deng, Yanhong; Yang, Anqi; Chen, Bin; Yin, Yulong; Gu, Jingjing; Xiang, Jiaojiao] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Peoples R China.
[Chen, Chujie; Tang, Xiangwei; Yan, Saina; Gu, JJ; Deng, Yanhong; Yang, Anqi; Chen, Bin; Gu, Jingjing; Xiang, Jiaojiao] Hunan Agr Univ, Hunan Prov Key Lab Genet Improvement Domest Anim, Changsha 410128, Peoples R China.
[Yin, Yulong] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Inst Subtrop Agr, Changsha 410125, Peoples R China.
通讯机构:
[Gu, JJ ; Chen, B] H
Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Peoples R China.
Hunan Agr Univ, Hunan Prov Key Lab Genet Improvement Domest Anim, Changsha 410128, Peoples R China.
语种:
英文
关键词:
6 n methyladenosine;activin receptor 2;alkylated DNA repair protein alkB homolog 5;aurora C kinase;cyclic AMP;G protein coupled receptor;hydroxymethylglutaryl coenzyme A reductase kinase;mammalian target of rapamycin;messenger RNA;phosphatidylinositol 3 kinase;protein kinase B;transcription factor Sox9;transcriptome;Wnt protein;phosphatidylinositol 3 kinase;RNA;transcriptome;animal cell;animal experiment;animal tissue;apoptosis;Article;cell proliferation;cell viability;chromosome 1;comparative study;consensus sequence;controlled study;epigenetic modification;gene expression;genetic transcription;high throughput sequencing;KEGG;male;maturity;methylome;nonhuman;oxidation;pig;protein expression;puberty;real time polymerase chain reaction;regulatory mechanism;reproduction;RNA immunoprecipitation;RNA interference;RNA methylation;RNA sequencing;Sertoli cell;sexual maturation;sexual maturity;signal transduction;spermatogenesis;spliceosome;start codon;stop codon;testicular cell line;testis development;testis tissue;animal;epigenome;metabolism;sexual maturation;testis;Animals;Epigenome;Male;Phosphatidylinositol 3-Kinases;RNA;Sexual Maturation;Swine;Testis;Transcriptome
期刊:
International Journal of Molecular Sciences
ISSN:
1661-6596
年:
2023
卷:
24
期:
19
页码:
14475
基金类别:
This work was financially supported by a key R&D project from Hunan Province (2020NK2024), an earmarked fund for the Hunan Provincial Modern Agro-Industry Technology Research System, and the Hunan Provincial Natural Science Foundation of China (2020JJ4348).
机构署名:
本校为第一且通讯机构
院系归属:
动物科学技术学院
摘要:
RNA N6-methyladenosine (m6A) modification is one of the principal post-transcriptional modifications and plays a dynamic role in testicular development and spermatogenesis. However, the role of m6A in porcine testis is understudied. Here, we performed a comprehensive analysis of the m6A transcriptome-wide profile in Shaziling pig testes at birth, puberty, and maturity. We analyzed the total transcriptome m6A profile and found that the m6A patterns were highly distinct in terms of the modification of the transcriptomes during porcine testis deve...

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