期刊论文

Liu, Hu-Hu

英文

Bioresource Technology

0960-8524

2021

323

124652

10.1016/j.biortech.2020.124652

The authors thank Prof. Xiao-Jun Ji (Nanjing Tech University) for providing us the Y. lipolytica strain Po1f (Leu−, Ura−, Δku70). This work was supported by the National Natural Science Foundation of China (No. 21808052 ), the China Postdoctoral Science Foundation (No. 2019TQ0088 ), the Scientific Research Foundation of Hunan Provincial Education Department (Nos. 18B090 and 18K039 ) and the Double First-Class Construction Project of Hunan Agricultural University (No. SYL201802002 ).

本校为第一且通讯机构

The aim of this present research is to enhance the squalene production in Yarrowia lipolytica using pathway engineering and bioprocess engineering. Firstly, to improve the production of squalene, the endogenous HMG-CoA reductase (HMG1) was overexpressed in Y. lipolytica to yield 208.88 mg/L squalene. Secondly, the HMG1 and diacylglycerol acyltranferase (DGA1) were co-overexpressed, the derived recombinant Y. lipolytica SQ-1 strain produced 439.14 mg/L of squalene. Thirdly, by optimizing the fermentation medium, the improved titer of squalene wi...