Metabolic engineering of Yarrowia lipolytica for high-level production of squalene

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成果类型：

期刊论文

作者：

Liu, Ziying;Huang, Mingkang;Chen, Hong;Lu, Xiangyang;Tian, Yun;...

通讯作者：

Liu, HH

作者机构：

[Tian, Yun; Zhao, Qiaoqin; Chen, Hong; Liu, Huhu; Lu, Xiangyang; Liu, Ziying; Liu, HH; Huang, Mingkang; Hu, Pengcheng] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

[Li, Changzhu; Tian, Yun; Li, Peiwang] Hunan Acad Forestry, State Key Lab Utilizat Woody Oil Resource, Changsha 410004, Hunan, Peoples R China.

[Ji, Xiaojun] Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, Nanjing 211816, Jiangsu, Peoples R China.

通讯机构：

[Liu, HH ] H

Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

语种：

英文

关键词：

Fermentation;Metabolic Engineering;Saccharomyces cerevisiae;Squalene;Triterpenes;Yarrowia;Metabolism;Yeast;atipk protein;cahmg1 protein;diacylglycerol;diacylglycerol acyltransferase;fat droplet;fatty acid ester;genomic DNA;glucose 6 phosphate dehydrogenase;high mobility group B1 protein;hydroxymethylglutaryl coenzyme A reductase;isoprenaline;lhchk protein;reduced nicotinamide adenine dinucleotide phosphate;schmg1 protein;slhmg1 protein;squalene;squalene monooxygenase;squalene synthase;terbinafine;triacylglycerol;unclassified drug;yldga1 protein;yldga2 protein;ylg6pd protein;ylhmg1 protein;ylpgd protein;ylsqs protein;squalene;triterpene;Growing markets;High-level production;Isoprenol;Isoprenol utilization pathway;Lipid droplets;Squalene;Systems metabolic engineerings;Triterpenes;Y. lipolytica;Yarrowia lipolytica;cell;fermentation;gene expression;lipid;microbial activity;Article;bioengineering;concentration process;controlled study;fermentation optimization;gene overexpression;metabolic engineering;nonhuman;plasmid;Saccharomyces cerevisiae;signal transduction;synthesis;Yarrowia lipolytica;fermentation;genetics;metabolic engineering;metabolism;procedures;Yarrowia;Metabolic engineering

期刊：

Bioresource Technology

ISSN：

0960-8524

年：

2024

卷：

394

页码：

130233

DOI：

10.1016/j.biortech.2023.130233

基金类别：

Foundation of State Key Laboratory of Utilization of Woody Oil Resource, (GZKF202119) Hunan Agricultural University Graduate Student Research and Innovation Program, (2023XC094) Hunan Science and Technology Talent Lifting Project - Young and Middle-Aged Excellent Science and Technology Talent Training Program, (2023TJ-Z04) Science and Technology Innovation Program of Forestry in Hunan Province, (XLKY202310) National Natural Science Foundation of China, NSFC, (21808052, kq2208077) Natural Science Foundation of Hunan Province, (2022JJ50022) Training Program for Excellent Young Innovators of Changsha, (kq2206037) Science and Technology Program of Hunan Province, (2022RC1170)

机构署名：

本校为第一且通讯机构

院系归属：

生物科学技术学院

摘要：

Squalene is an important triterpene with a wide range of applications. Given the growing market demand for squalene, the development of microbial cell factories capable of squalene production is considered a sustainable method. This study aimed to investigate the squalene production potential of Yarrowia lipolytica. First, HMG-CoA reductase from Saccharomyces cerevisiae and squalene synthase from Y. lipolytica was co-overexpressed in Y. lipolytica. Second, by enhancing the supply of NADPH in the squalene synthesis pathway, the production of squalene in Y. lipolytica was effectively increased. ...

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Metabolic engineering of Yarrowia lipolytica for high-level production of squalene

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