期刊论文

Liu, HH

英文

Bioresource Technology

0960-8524

2024

394

130233

10.1016/j.biortech.2023.130233

National Natural Science Foundation of China [21808052]; Training Program for Excellent Young Innovators of Changsha [kq2206037]; Science and Technology Innovation Program of Hunan Province [2022RC1170]; Natural Science Foundation of Changsha [kq2208077]; Hunan Provincial Natural Science Foundation of China [2022JJ50022]; Foundation of State Key Laboratory of Utilization of Woody Oil Resource [GZKF202119]; Hunan Science and Technology Talent Lifting Project-Young and Middle -Aged Excellent Science and Technology Talent Training Program [2023TJ-Z04]; Science and Technology Innovation Program of Forestry in Hunan Province [XLKY202310]; Hunan Agricultural University Graduate Student Research and Innovation Program [2023XC094]

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Squalene is an important triterpene with a wide range of applications. Given the growing market demand for squalene, the development of microbial cell factories capable of squalene production is considered a sustainable method. This study aimed to investigate the squalene production potential of Yarrowia lipolytica. First, HMG-CoA reductase from Saccharomyces cerevisiae and squalene synthase from Y. lipolytica was co-overexpressed in Y. lipolytica. Second, by enhancing the supply of NADPH in the squalene synthesis pathway, the production of squalene in Y. lipolytica was effectively increased. ...