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One-step immobilization antibodies using ferrocene-containing thiol aromatic aldehyde for the fabrication of a label-free electrochemical immunosensor

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成果类型:
期刊论文
作者:
Liu, Xiaoying*;Li, Qiang;Chen, Lijuan;Zhou, Jiheng;Liu, Meiling;...
通讯作者:
Liu, Xiaoying;Shen, Youming
作者机构:
[Li, Qiang; Liu, Xiaoying; Zhou, Jiheng; Chen, Lijuan] Hunan Agr Univ, Sci & Technol Innovat Platform, Coll Sci, Changsha 410128, Hunan, Peoples R China.
[Shen, Youming] Hunan Univ Arts & Sci, Coll Chem & Chem Engn, Changde 415000, Peoples R China.
[Liu, Meiling] Hunan Normal Univ, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ, Coll Chem & Chem Engn, Changsha 410081, Hunan, Peoples R China.
通讯机构:
[Liu, Xiaoying; Shen, Youming] H
Hunan Agr Univ, Sci & Technol Innovat Platform, Coll Sci, Changsha 410128, Hunan, Peoples R China.
Hunan Univ Arts & Sci, Coll Chem & Chem Engn, Changde 415000, Peoples R China.
语种:
英文
期刊:
RSC Advances
ISSN:
2046-2069
年:
2016
卷:
6
期:
115
页码:
114019-114023
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21342012, 21305042]; Scientific Research Fund of Hunan provincial Science and Technology Department [14JJ4030]; Opening Fund of Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Hunan Normal University) Ministry of Education [KLCBTCMR2011-08]; Introduce Talents Fund of Hunan Agricultural University [13YJ01]
机构署名:
本校为第一且通讯机构
院系归属:
理学院
摘要:
This work focuses on a facile method for antibody immobilization to fabricate a label-free electrochemical immunosensor using ferrocene-containing thiol aromatic aldehyde (FcSA) synthesized by us. The FcSA plays important roles as follows: provides a novel self-assembled monolayer and the aldehyde group was applied to immobilize antibodies directly, in which no additional activation step was required. Ferrocene can improve the electrical conductivity of the sensing interface as compared to the ferrocene-free thiol aromatic aldehyde. Human IgG as a model analyte was detected in the range from 0...

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