期刊论文

Shen, GL

英文

Analytica Chimica Acta

0003-2670

2003

494

1-2

177-185

10.1016/S0003-2670(03)00988-7

This work was supported by the National Natural Science Foundation of China (Grants Nos. 29975006, 20075006, 70171055, 50179011), the Foundation of the National 863 High Technologies Research of China (No. 2001AA644020), and the Foundation of Hunan Province (Grants Nos. 01JJY1003, 01JJY2099).

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A rapid, convenient and accurate method using a sol-gel-alginate-carbon composite electrode (SACE) is described for the determination of β-indole acetic acid (IAA) in plant samples. The determination is based on an enzyme-linked competitive immunoreaction between free IAA and IAA labeled with HRP to bind on the anti-IAA IgG immobilized on the SACE surface. The response signal expressed as percentage current reduction (CR%, y) is linearly related to the logarithm of the concentration of IAA (x) in range of 5-500μgml -1 with a regression equati...