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Analysis of oral microorganism diversity in healthy individuals before and after chewing areca nuts using PCR-denatured gradient gel electrophoresis

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成果类型:
期刊论文
作者:
Xiong, Xiong;Hou, Aixiang;Yi, Shuhan;Guo, Yichen;Zhao, Ziwei;...
通讯作者:
Ren, Youhua;Wang, Yuanliang
作者机构:
[Wu, Zhongkun; Ren, Youhua; Wang, YL; Xiong, Xiong; Li, Zongjun; Li, Ke; Hou, Aixiang; Guo, Yichen; Cheng, Huan; Wang, Yuanliang; Zhao, Ziwei; Yi, Shuhan] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
[Li, Ke] Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
[Li, Ke] Engn Res Ctr Union Hainan Hunan Prov Areca Nut In, Haikou 570100, Hainan, Peoples R China.
通讯机构:
[Ren, YH; Wang, YL] H
Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
语种:
英文
关键词:
Areca nuts;Microbial diversity;Oral microorganism;PCR-DGGE;Saliva
期刊:
动物营养(英文)
ISSN:
2405-6545
年:
2018
卷:
4
期:
3
页码:
294-299
基金类别:
This research was funded by the Hainan Province Science and Technology Major Project (No. ZDKJ2016003 ), the Hainan Province Applied Technology R&D and Demonstration Project (No. ZDXM2015013 ), the Hunan Province Xiangtan City Key Project (No. NY-ZD20161002 ), and the Xiangtan Science and Technology Commissioner Project (No. CXY-TPZJ20171020 ). The authors thank the staff at Hunan Qingke Biotech Co., Ltd. and Professor Yuan-Liang Wang's experimental team from the College of Food Science and Technology, Hunan Agricultural University for their assistance.
机构署名:
本校为第一且通讯机构
院系归属:
食品科学技术学院
摘要:
To analyze oral microbial diversity in the saliva of 8 healthy individuals before and after chewing areca nuts. Saliva samples were collected before chewing areca nuts, after chewing areca nuts for 5 min and after chewing areca nuts for 30 min. DNA was extracted, and microbial diversity was examined using PCR-denaturing gradient gel electrophoresis (PCR-DGGE). When examining DGGE profiles collectively, the bands associated with Streptococcus and Veillonella were the most intense, making them the most prevalent bacteria. Furthermore, the band in...

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