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Analysis of oral microorganism diversity in healthy individuals before and after chewing areca nuts using PCR-denatured gradient gel electrophoresis

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成果类型:
期刊论文
作者:
Xiong, Xiong;Hou, Aixiang;Yi, Shuhan;Guo, Yichen;Zhao, Ziwei;...
通讯作者:
Ren, Youhua;Wang, Yuanliang
作者机构:
[Wu, Zhongkun; Ren, Youhua; Wang, YL; Xiong, Xiong; Li, Zongjun; Li, Ke; Hou, Aixiang; Guo, Yichen; Cheng, Huan; Wang, Yuanliang; Zhao, Ziwei; Yi, Shuhan] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
[Li, Ke] Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
[Li, Ke] Engn Res Ctr Union Hainan Hunan Prov Areca Nut In, Haikou 570100, Hainan, Peoples R China.
通讯机构:
[Ren, YH; Wang, YL] H
Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
语种:
英文
关键词:
Areca nuts;Microbial diversity;Oral microorganism;PCR-DGGE;Saliva
期刊:
动物营养(英文)
ISSN:
2405-6545
年:
2018
卷:
4
期:
3
页码:
294-299
基金类别:
This research was funded by the Hainan Province Science and Technology Major Project (No. ZDKJ2016003 ), the Hainan Province Applied Technology R&D and Demonstration Project (No. ZDXM2015013 ), the Hunan Province Xiangtan City Key Project (No. NY-ZD20161002 ), and the Xiangtan Science and Technology Commissioner Project (No. CXY-TPZJ20171020 ). The authors thank the staff at Hunan Qingke Biotech Co., Ltd. and Professor Yuan-Liang Wang's experimental team from the College of Food Science and Technology, Hunan Agricultural University for their assistance.
机构署名:
本校为第一且通讯机构
院系归属:
食品科学技术学院
摘要:
To analyze oral microbial diversity in the saliva of 8 healthy individuals before and after chewing areca nuts. Saliva samples were collected before chewing areca nuts, after chewing areca nuts for 5 min and after chewing areca nuts for 30 min. DNA was extracted, and microbial diversity was examined using PCR-denaturing gradient gel electrophoresis (PCR-DGGE). When examining DGGE profiles collectively, the bands associated with Streptococcus and Veillonella were the most intense, making them the most prevalent bacteria. Furthermore, the band intensities did not decrease after chewing areca nut...

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