Molecular characterization of a Chinese isolate of potato virus A (PVA) and evidence of a genome recombination event between PVA variants at the 3\'-proximal end of the genome. - 成果

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Molecular characterization of a Chinese isolate of potato virus A (PVA) and evidence of a genome recombination event between PVA variants at the 3'-proximal end of the genome.

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成果类型：

期刊论文

作者：

He, Changzheng;Zhang, Wei;Hu, Xinxi;Singh, Mathuresh;Xiong, Xingyao*;...

通讯作者：

Xiong, Xingyao

作者机构：

[Hu, Xinxi; Zhang, Wei; Xiong, Xingyao; He, Changzheng] Hunan Agr Univ, Coll Hort & Landscape, Hunan Prov Engn Res Ctr Potatoes, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.

[Singh, Mathuresh] Agr Certificat Serv, Fredericton, NB E3B 8B7, Canada.

[Nie, Xianzhou] Agr & Agri Food Canada, Potato Res Ctr, Fredericton, NB E3B 4Z7, Canada.

通讯机构：

[Xiong, Xingyao] H

Hunan Agr Univ, Coll Hort & Landscape, Hunan Prov Engn Res Ctr Potatoes, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.

语种：

英文

关键词：

Potato Virus;Mosaic Symptom;Recombination Breakpoint;Chinese Isolate;Recombination Detection Program

期刊：

Archives of Virology

ISSN：

0304-8608

年：

2014

卷：

159

期：

页码：

2457-2462

DOI：

10.1007/s00705-014-2053-z

基金类别：

This research was supported by the National High Technology Research and Development Program (Project no. 2013AA102603), Hunan Provincial Natural Science Foundation (Project no. 13JJ2029), and the Ministry of Science and Technology of China through the ISTP Canada-China Science and Technology program (Project no. CCRD07-077). The authors thank Virginia Dickson for careful proofreading of the manuscript prior to submission.

机构署名：

本校为第一且通讯机构

院系归属：

农学院

园艺园林学院

摘要：

Potato plants that exhibited mosaic symptoms were collected in Xiangxi, Hunan province, China. Multiplex RT-PCR screening for common viruses revealed the presence of potato virus A (PVA) in these samples. ELISA with virus-specific antibodies confirmed infection by PVA in the plants. Rod-shaped virions of ~750 nm in length and ~13 nm in width were observed by transmission electron microscopy. One virus isolate (designated PVA-Hunan) was subjected to molecular characterization. The viral genome consisted of 9,567 nucleotides, excluding the poly(A) tail, and encoded a polyprotein of 3,059 amino a...

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Molecular characterization of a Chinese isolate of potato virus A (PVA) and evidence of a genome recombination event between PVA variants at the 3'-proximal end of the genome.

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