Molecular characterization of Potato mop-top virus isolates from China and Canada and development of RT-PCR differentiation of two sequence variant groups

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成果类型：

期刊论文

作者：

Hu, Xinxi;Dickison, Virginia;Lei, Yan;He, Changzheng;Singh, Mathuresh;...

通讯作者：

Nie, Xianzhou

作者机构：

[Hu, Xinxi; Lei, Yan; Xiong, Xingyao; He, Changzheng] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Hunan Prov Engn Res Ctr Potatoes, Collaborat Innovat Ctr Grain & Oil Crops South Ch, Changsha 410128, Hunan, Peoples R China.

[Dickison, Virginia; Nie, Xianzhou] Agr & Agri Food Canada, Fredericton Res & Dev Ctr, 850 Lincoln Rd,POB 20280, Fredericton, NB E3B 4Z7, Canada.

[Singh, Mathuresh] Agr Certificat Serv Inc, 1030 Lincoln Rd, Fredericton, NB E3B 8B7, Canada.

[Yang, Yanli] Yunnan Agr Univ, Coll Plant Protect, Kunming 650201, Yunnan, Peoples R China.

通讯机构：

[Nie, Xianzhou] A

Agr & Agri Food Canada, Fredericton Res & Dev Ctr, 850 Lincoln Rd,POB 20280, Fredericton, NB E3B 4Z7, Canada.

语种：

英文

关键词：

genomic RNA reassortment;molecular diversity;phylogenetic analysis;Potato mop-top virus;RT-PCR differentiation;sequence analysis;Virus du sommet touffu de la pomme de terre;analyse de séquence;diversité moléculaire;réarrangement de l’ARN génomique;analyse phylogénétique;différenciation par RT-PCR

期刊：

Canadian Journal of Plant Pathology

ISSN：

0706-0661

年：

2016

卷：

期：

页码：

231-242

DOI：

10.1080/07060661.2016.1189968

基金类别：

Ministry of Agriculture of ChinaMinistry of Agriculture, China [2012-03096]; Agriculture and Agri-Food CanadaAgriculture & Agri Food Canada [1110]

机构署名：

本校为第一机构

院系归属：

农学院

园艺园林学院

摘要：

The complete genome comprising three genomic RNAs of three Canadian and two Chinese isolates of Potato mop-top virus were sequenced and analysed. Two open reading frames (ORFs) were found in RNA1 of 6.1 kb, encoding a readthrough RNA-dependent RNA polymerase (RdRp). A coat protein (CP)-readthrough protein was encoded by RNA2 of 3.1 kb. Four ORFs that encoded the triple gene block proteins (TGBps) and a cysteine-rich protein were found in RNA3 (2.9 kb) of the Chinese isolate ‘Yunnan’; whereas in the remaining isolates (three Canadian isolates ...

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Molecular characterization of Potato mop-top virus isolates from China and Canada and development of RT-PCR differentiation of two sequence variant groups

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