期刊论文

Cheng, Tian-Yin

英文

Experimental and Applied Acarology

0168-8162

2018

74

2

171-176

10.1007/s10493-018-0210-x

Acknowledgements This work was supported in part by Scientific Research Fund of Hunan Provincial Education Department (No. 16A102), and the National Natural Science Foundation of China (No. 31372431).

本校为第一且通讯机构

In the present study, the entire first and second internal transcribed spacer (ITS-1 and ITS-2) regions of nuclear ribosomal DNA (rDNA) of Haemaphysalis longicornis from China were amplified by polymerase chain reaction. The 45 representative amplicons were sequenced, and sequence variation in the ITS was examined. The ITS sequences of H. longicornis were 3644 bp in size, including the part of 18S rDNA, 28S rDNA sequences and the complete ITS-1, 5.8S rDNA and ITS-2 sequences. Sequence analysis revealed that the ITS-1, 5.8S rDNA and ITS-2 of thi...