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HSC70 from Haemaphysalis flava (Acari: Ixodidae) exerts anticoagulation activity in vitro.

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成果类型:
期刊论文
作者:
He, Xiao-ming;Liu, Lei*;Cheng, Tian-yin*
通讯作者:
Liu, Lei;Cheng, Tian-yin
作者机构:
[He, Xiao-ming] College of Veterinary Medicine, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Hunan Collaborative Innovation Center for Safety Production of Livestock and Poultry, Hunan Agricultural University, Changsha, 410128, China
[Cheng, Tian-yin] College of Veterinary Medicine, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Hunan Collaborative Innovation Center for Safety Production of Livestock and Poultry, Hunan Agricultural University, Changsha, 410128, China. Electronic address: hn5368@163.com
[Liu, Lei] College of Veterinary Medicine, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Hunan Collaborative Innovation Center for Safety Production of Livestock and Poultry, Hunan Agricultural University, Changsha, 410128, China. Electronic address: ll434@hunau.edu.cn
通讯机构:
[Cheng, Tian-Yin; Liu, Lei] College of Veterinary Medicine, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Hunan Collaborative Innovation Center for Safety Production of Livestock and Poultry, Hunan Agricultural University, Changsha, 410128, China. Electronic address:
语种:
英文
关键词:
Tick;HSC70;Heat shock protein;Anticoagulation
期刊:
Ticks and tick-borne diseases
ISSN:
1877-959X
年:
2019
卷:
10
期:
1
页码:
170-175
文献类别:
WOS:Article
所属学科:
WOS学科类别:Infectious Diseases;Microbiology;Parasitology
入藏号:
WOS:000455723900028;PMID:30366643
基金类别:
National Natural Science Foundation of China [31372431]; State Key Laboratory of Veterinary Etiological Biology [SKLVEB2016KFKT009]
机构署名:
本校为第一且通讯机构
院系归属:
动物医学院
摘要:
Ticks and tick-borne diseases are major global health threats. During blood feeding, ticks insert their hypostomes into hosts and inject an array of anticoagulant molecules to maintain fluidity of the blood-meal. These anticoagulant molecules may provide insights into understanding the feeding biology of ticks and to develop vaccines against infestations. In Haemaphysalis flava, the heat shock cognate 70 (HSC70), a member of the heat shock protein (HSP) family, is differentially expressed in salivary glands at different levels of engorgement during blood feeding. However, its function in ticks is largely not known. The present study was designed to explore the possible effects of HSC70 on the plasma. The open reading frame (ORF) of HSC70 was expressed in a prokaryotic system, and recombinant HSC70 (rHSC70) was purified and characterized. The anticoagulation activity of rHSC70 was estimated by measuring prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB) with/without its inhibitor, VER155008. The results demonstrated that rHSC70 from H. flava extended TT (P < 0.001) and FIB clotting times (>300 s), but showed little effect on PT and APTT. Adding an inhibitor reversed anticlotting effects of rHSC70 on TT and FIB. These data indicate that rHSC70 is an anticoagulant agent, and the anticlotting activity likely attributes to the inhibition of thrombin and the transformation of fibrinogen into fibrin.
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BOORSTEIN WR, 1994, J MOL EVOL, V38, P1
Ciprandi A, 2006, EXP PARASITOL, V114, P40, DOI 10.1016/j.exppara.2006.02.010
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