期刊论文

Re-sequencing Study on the Mitochondrial DNA of Tobacco Sterile Line and Maintainer Line

中文

天津农业科学

1006-6500

2016

22

6

1-8

10.3969/j.issn.1006-6500.2016.06.001

本校为第一机构

基于重测序技术,对两对不育系和对应的保持系材料的线粒体DNA进行重测序,统计并分析了不育系与保持系线粒体DNA编码区序列的突变位点,探讨了这些突变位点与烟草胞质不育的关系。对重测序结果比对分析可知：不育系和保持系在orf106b上具有一致的1个In Del位点,在orf138a、orf121b、orf129b和orf110b上具有一致的SNP位点,这些突变位点没有造成不育系与保持系编码区序列的差异,推测这些突变位点可能与CMS无关;不育系MSK326和MSK394在orf112a和orf103c上有相同的In Del位点;MSK326和MSK394在65个基因片段或开放阅读框上有一致SNP位点,且这些突变没有出现在保持系线粒体编码区。这些基因片段或开放阅...

Based on the technology of re-sequencing in this study, resequencd the mitochondrial DNA of two pairs of tobacco material of sterile line and maintainer line, statisticed analyzed the differences in mitochondrial DNA sequence of sterile line and maintainer line, discussed the relationship between the mutation site and CMS. For the comparison analysis of resequencing date in this samples,can know that： the sterile line and maintainer line had one same In Del site in orf106 b.The same SNP site were located in orf138 a,orf121b, orf129 b and orf11...