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Experimental evolution reveals nitrate tolerance mechanisms in Desulfovibrio vulgaris

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成果类型:
期刊论文
作者:
Wu, Bo;Liu, Feifei;Zhou, Aifen;Li, Juan;Shu, Longfei;...
通讯作者:
Yan, Qingyun;He, Zhili;Zhou, Jizhong;Chen, Shouwen
作者机构:
[Yan, Qingyun; Yang, Xueqin; Wu, Bo; Shu, Longfei; Yan, QY; He, Zhili] Sun Yat Sen Univ, Environm Microbi Res Ctr, Sch Environm Sci & Engn, Southern Marine Sci & Engn Guangdong Lab Zhuhai, Guangzhou 510006, Peoples R China.
[Van Nostrand, Joy D.; Yan, Qingyun; Wu, Bo; Kempher, Megan L.; Ning, Daliang; He, Zhili; Pan, Feiyan; Zhou, Jizhong; Liu, Feifei; Zhou, Aifen; Yan, QY; Zhou, JZ] Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA.
[Van Nostrand, Joy D.; Yan, Qingyun; Wu, Bo; Kempher, Megan L.; Ning, Daliang; He, Zhili; Pan, Feiyan; Zhou, Jizhong; Liu, Feifei; Zhou, Aifen; Yan, QY; Zhou, JZ] Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA.
[Liu, Feifei] Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, State Key Lab Appl Microbiol Southern China, Guangzhou 510070, Peoples R China.
[Li, Juan; He, Zhili] Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China.
通讯机构:
[Yan, QY; He, ZL] S
[Yan, QY; Zhou, JZ; He, ZL] U
[He, Zhili; Chen, Shouwen] H
[Zhou, Jizhong] L
[Zhou, Jizhong] T
语种:
英文
期刊:
ISME Journal
ISSN:
1751-7362
年:
2020
卷:
14
期:
11
页码:
2862-2876
基金类别:
Acknowledgements The experimental work of this study (October 2013 to June 2017) was largely supported by ENIGMA-Ecosystems and Networks Integrated with Genes and Molecular Assemblies (http://enigma.lbl.gov), a Science Focus Area Program at Lawrence Berkeley National Laboratory is based upon work supported by the U. S. Department of Energy, Office of Science, and Office of Biological & Environmental Research under contract number DE-AC02-05CH11231, the Office of the Vice President for Research at the University of Oklahoma (JZ), and the National Program on Key Basic Research Project of China (973 Program, No. 2015CB150505 to SC); the subsequent integration work (October 2018 to March 2020) of this study, including supplementary experiments, data analysis and manuscript writing, was mainly supported by the National Natural Science Foundation of China (Grant numbers 31770539 and 91951207 to ZH, grant number 31672262 to QY), and the Special Funds for Scientific and Technological Innovation in Guangdong Province (Grant number 2018A030310302 to BW).
机构署名:
本校为通讯机构
院系归属:
农学院
摘要:
Elevated nitrate in the environment inhibits sulfate reduction by important microorganisms of sulfate-reducing bacteria (SRB). Several SRB may respire nitrate to survive under elevated nitrate, but how SRB that lack nitrate reductase survive to elevated nitrate remains elusive. To understand nitrate adaptation mechanisms, we evolved 12 populations of a model SRB (i.e., Desulfovibrio vulgaris Hildenborough, DvH) under elevated NaNO3 for 1000 generations, analyzed growth and acquired mutations, and linked their genotypes with phenotypes. Nitrate-...

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