Rhipicephalus microplus salivary gland secretes a number of complex bioactive proteins during feeding. These components are important in feeding and affect anti-coagulation, anti-inflammation and also have anti-microbial effects. In this study, tick saliva was collected from partially engorged female (PEF) and fully engorged female (FEF) ticks. Liquid chromatography tandem–mass spectrometry (LC–MS/MS) and isobaric tags for relative and absolute quantification (iTRAQ) were used to identify and quantify R. microplus salivary proteins. A total o...