Comprehensive identification and structural characterization of target components from Gelsemium elegans by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry based on accurate mass databases combined with MS/MS spectra

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Liu, Yan-Chun;Xiao, Sa;Yang, Kun;Ling, Li;Sun, Zhi-Liang;...

通讯作者：

Liu, Zhao-Ying

作者机构：

[Sun, Zhi-Liang; Yang, Kun; Liu, Zhao-Ying; Liu, Yan-Chun; Ling, Li; Xiao, Sa] Hunan Agr Univ, Hunan Engn Res Ctr Vet Drug, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.

[Sun, Zhi-Liang; Liu, Zhao-Ying] Hunan Agr Univ, Coll Vet Med, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.

[Liu, Zhao-Ying] Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Hunan, Peoples R China.

[Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.

通讯机构：

[Liu, Zhao-Ying] H

Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.

语种：

英文

关键词：

accurate mass databases;Gelsemium;quadrupole time-of-flight mass spectrometry;traditional Chinese medicine;alkaloid

期刊：

JOURNAL OF MASS SPECTROMETRY

ISSN：

1076-5174

年：

2017

卷：

期：

页码：

378-396

DOI：

10.1002/jms.3937

基金类别：

Natural Science Foundation of Hunan Province, ChinaNatural Science Foundation of Hunan Province [2017JJ1017]

机构署名：

本校为第一且通讯机构

院系归属：

动物医学院

摘要：

This study reports an applicable analytical strategy of comprehensive identification and structure characterization of target components from Gelsemium elegans by using high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QqTOF MS) based on the use of accurate mass databases combined with MS/MS spectra. The databases created included accurate masses and elemental compositions of 204 components from Gelsemium and their structural data. The accurate MS and MS/MS spectra were acquired through data-dependent auto MS/MS mode followed by an extraction of the potenti...

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Comprehensive identification and structural characterization of target components from Gelsemium elegans by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry based on accurate mass databases combined with MS/MS spectra

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