期刊论文

中文

长江大学学报(自科版)医学卷

1673-1409

2009

6

2

56-60+1

10.3969/j.issn.1673-1409(S).2009.02.016

本校为第一机构

富贵菜(Gynura divaricata)离体培养研究结果表明,以幼叶为外植体,最适致密愈伤组织诱导培养基为改良MS + 6-BA 2.0 mg·L-1 + NAA 0.2 mg·L-1 + KT 0.4 mg·L-1 + 蔗糖3%,诱导率为95.9%;最适诱导增殖培养基为改良MS + 6-BA 3.0 mg·L-1 +NAA 0.1 mg·L-1 + 蔗糖3.0%,分化率为88%,增殖系数12.60;最适生根培养基为1/2改良MS + NAA 0.3 mg·L-1 + 蔗糖3%,生根率为100%;经该途径诱导得到的植株,经炼苗后移栽成活率≥93%.

In vitro culture and plant regeneration of Gynura divaricata by tissue culture using tis blade were studied. The results are as follows:the best medium for dense callus inducing is MS(improved) + 6-BA 2.0 mg·L-1 + NAA 2.0 mg·L-1 + KT 0.4 mg·L-1 + 3% sucrose,induction rate is 95.9%;for amplification is MS(improved) + 6-BA 3.0 mg·L-1 + NAA 0.1 mg·L-1 + 3% sucrose,rate of differentiation is 88%;root inducting is 1/2 MS(improved) + NAA 0.3 mg·L-1 + 3% Suc...