期刊论文

Comparison of PCR and Microscopic Examination of Giemsa-stained to Detect Mycoplasma suis

中文

长江大学学报(自科版)医学卷

1673-1409

2010

7

3

32-34+95+111

10.3969/j.issn.1673-1409(S).2010.03.012

[基金项目]湖南省科技厅资助项目（2009FJ3021） 湖南省教育厅资助项目（10C0809）

本校为第一机构

参照Hoelzle等报道的猪附红细胞体(Mycoplasma suis)PCR诊断方法,提取病原DNA进行PCR扩增,经克隆、测序得到781 bp片段,与Genbank中序列(登录号AJ504999)进行比较,同源性为99.4%.用该方法从大肠杆菌等猪常见病原基因组未扩增出相同的序列,表明其特异性好.以PCR和染色镜检方法分别检测3个猪场15份临床发病猪血液样品,M.suis阳性率均为100%,结果符合率为100%,差异不显著(P＞0.05);PCR检测和涂片染色镜检5个猪场25份临床健康猪血样,M. suis阳性率分别为100%和68.0%,二者符合率为68.0%,差异显著(P＜0.05).

A 781 bp DNA fragment was amplified and cloned and sequenced from genome of agent from pig’s blood according to the established methods of Hoelzle et al.A homology search was carried out with the databases of GenBank,the similarity to the known sequences(accession numbers AJ504999)was 99.4%.The specificity was demonstrated by PCR/RT-PCR from genome of various pig common pathogeny such as E coli et al.The blood samples obtained from 15 none-symptomatic pigs with clinical signs typical of porcine eperythrozoonosis(PE)were detected by PCR and mic...