期刊论文

Studies on Chinese medicine resistance inhibitors back mutation to Resistant Staphylococcus aureus gyrA genemutation

中文

中国兽医杂志

0529-6005

2013

49

9

3-5

10.3969/j.issn.0529-6005.2013.09.001

科技部国际科技合作计划专项基金（2009DFA32350）；

本校为第一机构

探讨金黄色葡萄球菌对氟喹诺酮类的耐药与gyrA基因突变的关系及中药耐药抑制剂对gyrA基因是否具有回复突变作用,以便从分子水平初步探讨复方中药耐药抑制剂的耐药抑制分子机理.用诺氟沙星逐步诱导金黄色葡萄球菌敏感株ATCC29213,建立了MIC值为64 μg/mL、128 μg/mL和256 μg/mL的耐药突变株；PCR扩增标准株、耐药株和中药处理的耐药株gyrA基因,产物回收纯化后送测序公司测序.gyrA基因测序结果用DNAsis软件分析表明,细菌的耐药性程度越高,碱基突变率增加；gyrA基因氨基酸序列突变分析表明,84位亲水性丝氨酸被疏水性缬氨酸取代,与细菌的高水平耐药性密切相关.中药耐药抑制剂对84位和99位氨基酸突...

To investigate the Staphylococcus aureus resistance to fluoroquinolones with gyrA gene mu- tation and reverting role of Chinese medicine resistance inhibitor. Methods. susceptible strains of standard Staphrlococcus aureus with drug-induced a series of norfloxacin-resistance were artificially induced to resist fung. PCR amplification for gyrA of resistant strains in the treatment of resistant strains and Chinese med- icine resistance inhibitor, processing gene sequencing and sequence analysis. Result. The results show that., the higher the degree of bacterial resistance, the more mutation rate ...