期刊论文

中文

中国兽医杂志

0529-6005

2017

53

4

27-30

30972191:国家自然科学基金 201523:吉林省留学人员创新创业项目 20130215:长春市农业先进实用技术的示范推广项目 2014Z34:引进国际先进农业科学技术计划计划)

本校为第一机构

为探寻猪场仔猪乙型脑炎抗体消长与蚊子携带乙型脑炎病毒的之间的关系,4月份选取一批约15日龄仔猪共24头,每隔20 d采血1次,至7月共采血6次,用间接ELISA方法检测血清乙型脑炎抗体,且不同时间在跟踪猪舍共捕获约3720只蚊子,用RT-PCR方法鉴定乙型脑炎病毒核酸;结果表明,该批猪乙型脑炎抗体阳性率总体呈现先下降后升高规律,阳性率先后为83.3%、66.7%、41.6%、25%、50%、91.6%,生猪体内母源抗体水平在75日龄（6月20日）达到最低,阳性率降至25%,然后呈现明显的上升趋势,115日龄（7月30日）检测阳性率高达91.6%;37份蚊子样品,JEV核酸阳性样品16份,阳性率高达43.2%,其中7月份所采集14份蚊子样品检出7份,...

To investigate the link between the dynamical changes of JEV in piglets and JEV in mosquitos. Twenty - four 15 - day old piglets were selected and blood samples were collected 6 times every 20 days. Then, we used indirect EL/SA method to detect serum antibody against JEV. At the same time, a total of 3720 mosquitos were trapped, and Japanese encephalitis virus nucleic acid was detected by RT - PCR. Results showed that the positive rate of antibody against Japanese encephalitis Bwas declined at the beginning and then increased. The positive rate for 83.6%, 83.3%, 66. 7%, 41.6%, 25%, 50%, and 91...