Rapid on-site PEDV detection using homogeneous fluorescence resonance energy transfer-based ELISA

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成果类型：

期刊论文

作者：

Shan, Yanke;Feng, Yufan;Li, Jiahao;Yi, Weijie;Ge, Meng;...

通讯作者：

Shouyu Wang

作者机构：

[Li, Jiahao; Yi, Weijie; Liu, Fei; Shan, Yanke; Feng, Yufan; Wang, Shouyu] Nanjing Agr Univ, Joint Int Res Lab Anim Hlth & Food Safety, Minist Educ, Nanjing 210095, Jiangsu, Peoples R China.

[Li, Jiahao; Yi, Weijie; Liu, Fei; Shan, Yanke; Feng, Yufan; Wang, Shouyu] Nanjing Agr Univ, Single Mol Nanobiol Lab Sinmolab, Nanjing 210095, Jiangsu, Peoples R China.

[Ge, Meng] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.

[Huang, Huachuan] Southwest Univ Sci & Technol, Sch Manufacture Sci & Engn, Key Lab Testing Technol Mfg Proc, Minist Educ, Mianyang 621010, Sichuan, Peoples R China.

[Yan, Keding] Xian Technol Univ, Adv Inst Micronano Intelligent Sensing AIMNIS, Sch Elect Informat Engn, Xian 710032, Shaanxi, Peoples R China.

通讯机构：

[Shouyu Wang] J

Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education & Single Molecule Nanobiology Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, Jiangsu 210095, China<&wdkj&>OptiX+ Laboratory, School of Electronics and Information Engineering, Wuxi University, Wuxi, Jiangsu 214105, China

语种：

英文

关键词：

PEDV detection;FRET immunoassay;Rapid on-site detection;FRET immunoassay station (FRETIS)

期刊：

Sensors and Actuators B-Chemical

ISSN：

0925-4005

年：

2023

卷：

378

页码：

133138

DOI：

10.1016/j.snb.2022.133138

基金类别：

China Postdoctoral Science Foundation [2020M681649, 2020T130055ZX]; Jiangsu Postdoctoral Research Foundation [2020Z120]; Fundamental Research Funds for the Central Universities [KJQN202132]; National Natural Science Foundation of China [32000028]

机构署名：

本校为其他机构

院系归属：

动物医学院

摘要：

The porcine epidemic diarrhea virus (PEDV) is a major pathogen of swine enteric diseases. Various etiology and serological methods have been employed for PEDV detection, but most of their applications are limited to lab-oratories. To extend PEDV detection to on-site applications, we design a homogeneous fluorescence resonance energy transfer (FRET)-based enzyme-linked immunosorbent assay (ELISA). Both donor and acceptor fluores-cence microspheres modified PEDV antibodies can be linked only to the occurrence of PEDV antigen, thus generating FRET signals, which can be collected by our designed p...

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Rapid on-site PEDV detection using homogeneous fluorescence resonance energy transfer-based ELISA

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