期刊论文

中文

东北农业大学学报

1005-9369

2014

45

1

70-76

10.19720/j.cnki.issn.1005-9369.2014.01.012

2012CB723004:国家重点基础研究发展计划（973计划） 12C0164:湖南省教育厅一般项目 11QN11:湖南农业大学青年科学基金 11KFXM09:国家重点实验室基金

本校为第一机构

通过高压富集法,从土壤中分离筛选得到一株纤维素降解菌株,并对其木糖发酵特性进行初步研究。根据菌株的形态特性和生理生化特征,初步鉴定为好食脉孢菌(Neurosporasitophila)。结果表明,该菌株CMC 酶活力为3 554.888U.g~(-1)、FPA酶活力为506.76 U.g~(-1),当紫外线照射剂量30W,照射距离20cm,照射时间为8min 时,致死率为77.28%。在木糖浓度20g.L~(-1)、发酵温度25℃、发酵时间96h、转速140 r.min~(-1)的条件下乙醇产量可达5.5g.L~(-1)、乙醇得率为理论值的59.8%。

Using the high pressure enrichment method, one bacteria strian were separated from soil,which can identified as cellulose degradation strains, and the xylose fermentation characteristics were studied. According to the morphological characteristics and physiological and biochemical characteristics of strains this strian was initially identified as Neurospora sitophila. The strain of CMC enzyme activity of 3 554.88 U · g-1, FPA enzyme activity of 506.76 U· g-1, when ultraviolet irradiation dose 30 W, 20 cm distance, the irradiation time of 8 mi...