Application of high-resolution DNA melting for genotyping and variant scanning of diploid and autotetraploid potato

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期刊论文

作者：

De Koeyer, David*;Douglass, Katheryn;Murphy, Agnes;Whitney, Sean;Nolan, Lana;...

通讯作者：

De Koeyer, David

作者机构：

[Douglass, Katheryn; Murphy, Agnes; Nolan, Lana; Song, Yong; De Koeyer, David; Whitney, Sean] Agr & Agri Food Canada, Potato Res Ctr, Fredericton, NB E3B 4Z7, Canada.

[Nolan, Lana] Univ New Brunswick, Fac Forestry & Environm Management, Fredericton, NB E3B 6C2, Canada.

[Song, Yong] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.

[De Jong, Walter] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA.

[De Koeyer, David] Agr & Agri Food Canada, Potato Res Ctr, 850 Lincoln Rd, Fredericton, NB E3B 4Z7, Canada.

通讯机构：

[De Koeyer, David] A

Agr & Agri Food Canada, Potato Res Ctr, 850 Lincoln Rd, Fredericton, NB E3B 4Z7, Canada.

语种：

英文

关键词：

Potato;High-resolution DNA melting;Haplotype dosage;Autotetraploid genetics;Marker-assisted selection;Variant scanning

期刊：

Molecular Breeding

ISSN：

1380-3743

年：

2010

卷：

期：

页码：

67-90

DOI：

10.1007/s11032-009-9309-4

机构署名：

本校为其他机构

摘要：

The ideal marker system for tetraploid potato would be dosage-sensitive and have the ability to distinguish heterozygous genotypes with multiple haplotypes within the genomic region targeted by the marker. The objective of this study was to evaluate the utility of high-resolution DNA melting (HRM) for genotyping and polymorphism detection in diploid and tetraploid potato. Amplicon scanning, unlabelled probe, and short amplicon assays were developed for four candidate genes affecting tuber skin and flesh colour, and starch, and a marker linked to nematode resistance. Genotyping a set of 95 pota...

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Application of high-resolution DNA melting for genotyping and variant scanning of diploid and autotetraploid potato

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