期刊论文、会议论文

中文

食品科学

1002-6630

2009

30

14

114-118

光友“红薯之路”第二届国际研讨会

光友“红薯之路”第二届国际研讨会论文集

2010-04-15

北京

中国食品工业协会<&wdkj&>农业部农业贸易促进中心<&wdkj&>四川省农业科学院

10.3321/j.issn:1002-6630.2009.14.018

本校为第一机构

本实验以红薯叶为原料,研究了黄酮类化合物分离纯化工艺,并研究了粗提物与纯化产物对羟基自由基、1,1-二苯基-2-苦基肼自由基(OPPH·)的清除作用.结果表明,红薯叶黄酮最佳提取工艺为60%乙醇水溶液90℃回流提取60min,料液比1∶40,粗提物中总黄酮含量为101.3mg/g.提取液用HPD500大孔树脂进行纯化效果好,最佳纯化工艺为:吸附条件:上样原料液黄酮含量1.8mg/ml,pH3上样流速为3BV/h:洗脱条件:50%乙醇、流速3BV/h,流量4BV,在此条件下,纯化产品黄酮含量为488.7mg/g;粗提物与纯化产物均具有对自由基的清除作用,是一种有效的天然抗氧化剂新资源.

Except that few sweet potato leaves are taken as edible food or livestock feed, most of them that contain large amounts of bioactive flavonoids are discarded, which leads to a great waste of available resource. In the present study total flavonoids were extracted from sweet potato leaves using ethanol and purified using macroporous adsorption resin. The extraction and purification procedures were optimized by orthogonal array design and one-factor-at-a-time methods, respectively. Additionally, antioxidant properties of the purified total flavon...