通讯机构：
[Bai, Lian-Yang] Hunan Agr Univ, Pesticide Res Inst, Changsha 410128, Hunan, Peoples R China.
摘要：
The purpose of this study was to develop a sensitive and low-cost optical sensor for screening pymetrozine using unmodified gold nanoparticles (G NPs). The presence of pymetrozine could rapidly induce the aggregation of G NPs, thereby resulting in red to deep blue colour change. The concentration of pymetrozine was determined with a limit of detection lower than 1×10
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M by the naked eye and was further easily monitored by a UV-vis spectrometer. The highest selectivity was achieved in comparison with other 11 pesticides and the possible underlying mechanism was investigated in detail by the simultaneous addition of glutathione and pymetrozine in G NPs solution.
作者：
Yang, Zhongxia;Statler, Bethanie-Michelle;Calkins, Travis L.;Alfaro, Edna;Esquivel, Carlos J.;Rouhier, Matthew F.;Denton, Jerod S.;Piermarini, Peter M.*
期刊：
Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology,2017年204:35-44 ISSN：1096-4959
通讯作者：
Piermarini, Peter M.
作者机构：
[Yang, Zhongxia] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Pes, Coll Plant Protect, Changsha, Hunan, Peoples R China.;[Rouhier, Matthew F.; Alfaro, Edna; Piermarini, Peter M.; Calkins, Travis L.; Statler, Bethanie-Michelle; Yang, Zhongxia; Esquivel, Carlos J.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, 224 Thorne Hall, Wooster, OH 44691 USA.;[Rouhier, Matthew F.] Kenyon Coll, Dept Chem, Gambier, OH 43022 USA.;[Denton, Jerod S.] Vanderbilt Univ, Med Ctr, Dept Anesthesiol, Nashville, TN USA.;[Denton, Jerod S.] Vanderbilt Univ, Med Ctr, Dept Pharmacol, Nashville, TN 37232 USA.
通讯机构：
[Piermarini, Peter M.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, 224 Thorne Hall, Wooster, OH 44691 USA.
摘要：
Inward rectifier potassium (Kir) channels play fundamental roles in neuromuscular, epithelial, and endocrine function in mammals. Recent research in insects suggests that Kir channels play critical roles in the development, immune function, and excretory physiology of fruit flies and/or mosquitoes. Moreover, our group has demonstrated that mosquito Kir channels may serve as valuable targets for the development of novel insecticides. Here we characterize the molecular expression of 5 mRNAs encoding Kir channel subunits in the yellow fever mosquito, Aedes aegypti: Kin, Kir2A-c, Kir2B, Kir2B', and Kir3. We demonstrate that 1) Kir mRNA expression is dynamic in whole mosquitoes, Malpighian tubules, and the midgut during development from 4th instar larvae to adult females, 2) Kir2B and Kir3 mRNA levels are reduced in 4th instar larvae when reared in water containing an elevated concentration (50 mM) of KCI, but not NaCl, and 3) Kir mRNAs are differentially expressed in the Malpighian tubules, midgut, and ovaries within 24 h after blood feeding. Furthermore, we provide the first characterization of Kir mRNA expression in the anal papillae of 4th instar larval mosquitoes, which indicates that Kir2A-c is the most abundant. Altogether, the data provide the first comprehensive characterization of Kir mRNA expression in Ae. aegypti and offer insights into the putative physiological roles of Kir subunits in this important disease vector. (C) 2016 Elsevier Inc. All rights reserved.
摘要：
Brevibacillus brevis X23 is an appropriate biocontrol agent against bacterial wilt caused by Ralstonia solanacearum. We report herein the draft genome sequence (6,566,879 bp) and a circular plasmid (6,600 bp) of B. brevis X23, data which may be helpful for mining the antagonistic activity against R. solanacearum.
摘要：
The commercially available ferrocene-based diphosphine ligand (S-c,S-Fc)-TaniaPhos was found to be highly effective in the Rh-catalyzed asymmetric hydrogenation of 3-aryl-2-(phosphonomethyl)propenates. Excellent enantioselectivity (90-98% ee) and high catalytic activity (S/C up to 1000) have been achieved, which represents the best results reported so far. (C) 2011 Elsevier Ltd. All rights reserved.
摘要：
Ditylenchus destructor is distributed widely in China and causes considerable yield losses of potatoes. Genetic diversity and variation of this species was analysed using Inter-Simple Sequence Repeats (ISSR) markers. Second-stage juveniles from 16 populations were analysed with three primer pairs and 32 fragments were considered for the analysis. Diversity levels within populations were relatively high. Cluster analysis and principle coordinate analysis grouped the majority of the nematode populations into three main clusters. At the regional level, the AMOVA indicated that about 91.4% variations in the data set were from genotypic variations within populations, 5.0% variations due to regional differences, and the remaining 3.6% due to differences among populations within regions. Low levels of genetic variation among populations suggested an extensive gene flow among them. Amphimictic mode in natural populations and passive dispersal of nematodes by anthropogenic activities and natural means would probably be responsible for the results observed. It was shown that the ISSR marker was an efficient method for detecting the genetic structure of D. destructor populations at a macro geographical level.
作者：
Su, Qi;Xie, Wen;Wang, Shaoli;Wu, Qingjun;Ghanim, Murad;Zhang, Youjun*
期刊：
PLOS ONE,2014年9(3):e91802 ISSN：1932-6203
通讯作者：
Zhang, Youjun
作者机构：
[Su, Qi] Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;[Xie, Wen; Zhang, Youjun; Wang, Shaoli; Wu, Qingjun; Su, Qi] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100193, Peoples R China.;[Ghanim, Murad] Agr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel.
通讯机构：
[Zhang, Youjun] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100193, Peoples R China.
关键词：
Rickettsia,Nymphs,Fluorescent in situ hybridization,Population density,Cotton,Insects,Population dynamics,Tomatoes
摘要：
Bacterial symbionts often enhance the physiological capabilities of their arthropod hosts and enable their hosts to expand into formerly unavailable niches, thus leading to biological diversification. Many arthropods, including the worldwide invasive whitefly Bemisia tabaci, have individuals simultaneously infected with symbionts of multiple genera that occur in different locations in the host. This study examined the population dynamics of symbionts that are located in different areas within B. tabaci. While densities of Portiera and Hamiltonella (which are located in bacteriocytes) appeared to be well-regulated during host development, densities of Rickettsia (which are not located in bacteriocytes) were highly variable among individual hosts during host development. Host mating did not significantly affect symbiont densities. Infection by Tomato yellow leaf curl virus did not affect Portiera and Hamiltonella densities in either sex, but increased Rickettsia densities in females. High and low temperatures did not affect Portiera and Hamiltonella densities, but low temperature (15 degrees C) significantly suppressed Rickettsia densities whereas high temperature (35 degrees C) had little effect on Rickettsia densities. The results are consistent with the view that the population dynamics of bacterial symbionts in B. tabaci are regulated by symbiont location within the host and that the regulation reflects adaptation between the bacteria and insect.
摘要：
In this study, we isolated an endophytic quinclorac-degrading bacterium strain Q3 from the root of tobacco grown in quinclorac contaminated soil. Based on morphological characteristics, Biolog identification, and 16S rDNA sequence analysis, we identified strain Q3 as Bacillus megaterium. We investigated the effects of temperature, pH, inoculation size, and initial quinclorac concentration on growth and degrading efficiency of Q3. Under the optimal degrading condition, Q3 could degrade 93% of quinclorac from the initial concentration of 20 mg/L in seven days. We analyzed the degradation products of quinclorac using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The major degradation products by Q3 were different from those of previously identified quinclorac degrading strains, which suggests that Q3 may employ new pathways for quinclorac degradation. Our indoor pot experiments demonstrated that Q3 can effectively alleviate the quinclorac phytotoxicity in tobacco. As the first endophytic microbial that is capable of degrading quinclorac, Q3 can be a good bioremediation bacterium for quinclorac phytotoxicity.
摘要：
A new pseudoguaianolide 1 and two new guaiane-type sesquiterpene glucosides 2 and 3, were isolated from the aerial parts of Ambrosia artemisiifolia L together with two known sesquiterpene dilactones 4 and 5. The new compounds were determined on the basis of spectroscopic and chemical methods to be 3 beta-acetoxy-4 beta-hydroxy-1 alpha,7 alpha, 10 beta,11 alpha H-pseudoguaia-12,8 beta-olide (1), 1 beta,7 beta,9 beta,10 beta,13 alpha H-guaia-4(5)-en-12,6 beta-olide 9-O-beta-d-glucoside (2) and 4 beta-hydroxy-1 alpha,5 alpha,7 alpha,9 alpha H-guaia-10(14),11(13)-dien-12-acid 9-O-beta-d-glucoside (3). The isolated compounds were evaluated for cytotoxicity against human promyelocytic leukemia HL-60 cell lines in vitro, but were all inactive.
作者机构：
[Luo, Li-Bin; Zhou, Xiao-Mao] Hunan Agr Univ, Inst Pesticide, Changsha 410128, Hunan, Peoples R China.;[Hu, Xiang-Ping; Huang, Jia-Di; Zheng, Zhuo] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China.;[Zhang, Cheng-Lu] Liaoning Normal Univ, Sch Chem & Chem Engn, Dalian 116029, Peoples R China.
通讯机构：
[Hu, Xiang-Ping] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China.
摘要：
A series of new chiral phosphine-aminophosphine ligands have been prepared via a two- or three-step transformation from commercially available and inexpensive (S)-1-phenylethylamine, and successfully used in the rhodium-catalyzed asymmetric hydrogenation of various enamides, beta-dehydroamino acid esters, and dimethyl itaconate. The results show that the ligand structure plays an important influence on both the reactivity and enantioselectivity. Ligand 2d bearing a N-H proton and two F-atoms on the 3,5-positions of the phenyl ring of the aminophosphino moiety was most effective for the hydrogenation of enamides and (Z)-beta-aryl-beta-(acylamino)acrylates, whereas ligand lb showed the highest enantioselectivities in the hydrogenation of (Z)-beta-alkyl-beta-(acylamino)acrylates and dimethyl itaconate. (C) 2010 Elsevier Ltd. All rights reserved.
摘要：
The Monopis monachella species complex from China is revised, and its relationship to other species complexes of the genus Monopis is discussed with reference to morphological, and molecular evidence. Principal component analysis on all available specimens provided supporting evidence for the existence of three species, one of which is described as new: Monopis iunctio Huang & Hirowatari sp. nov. All species are either diagnosed or described, and illustrated, and information is given on their distribution and host range. Additional information is given on the biology and larval stages of Monopis longella. A preliminary phylogenetic study based on mitochondrial cytochrome c oxidase subunit I gene (CO1) sequence data and a key to the species of the M. monachella species complex in China is presented. The types of the new species have been deposited in the Institute of Entomology, Hunan Agricultural University, China and Entomological Laboratory, Osaka Prefecture University, Japan. (C) 2011 The Linnean Society of London, Zoological Journal of the Linnean Society, 2011, 163, 1-14.
摘要：
Reed lignocellulose was subjected to a steam explosion pretreatment to obtain a high conversion rate of sugar after subsequent enzymatic hydrolysis using a commercial cellulase mixture. Under conditions of differing temperature (200 °C, 220 °C and 240 °C) and residence time (2, 5, and 8 min), the effect of the pretreatment on the sugar yield from enzymatic hydrolysis was studied. The highest respective reducing sugar and glucose yields were 36.14% and 15.35% after 60-h enzymatic hydrolysis of reed straw that had been pretreated with a steam explosion at 220 °C for 5 min. Fourier transform infrared spectrophotometry (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) were used in this study to comprehensively investigate the steam explosion-induced changes in the organizational structure and morphological properties of reed straw to analyze the reason for the increased sugar yield from enzymatic hydrolysis after the steam explosion.
