通讯机构：
[Li, Youzhi] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China.;National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China.
摘要：
BACKGROUND: Chlorantraniliprole is a diamide insecticide widely used in China over the last 15 years. The fall armyworm (FAW), Spodoptera frugiperda, newly invaded China in 2019. The response of FAW to chlorantraniliprole deserves more attention, in the context of many destructive lepidopteran species are resistant to diamide insecticides and the patent on core chemical of chlorantraniliprole in China expired in August 2022. METHODS AND RESULTS: This study investigated the response profile in larvae under chlorantraniliprole-induced (LC(50)) stress using methods of bioassay, RNA-Seq and qPCR. We observed growth inhibition and lethal effects in FAW larvae, but at a relatively high LC(50) value compared to other several pests. Additionally, under chlorantraniliprole-induced stress, 3309 unigenes were found to be differentially expressed genes. The impacted genes included 137 encoding for detoxification enzymes, 29 encoding for cuticle proteins, and 20 key enzymes involved in the chitin metabolism, which all associated with metabolic resistance. Finally, we obtained the single nucleotide polymorphisms (SNPs) of two RyR genes, which are the target proteins for chlorantraniliprole. We also investigated the causes of the high LC(50) value in our FAW, which possibly related to the stabilized 4743M on SNP frequency of RyR. These findings documented the genetic background of RyR of FAW and indicated that application of chlorantraniliprole has a high risk of controlling FAW in China. CONCLUSION: In brief, our results provide a better understanding of the mechanisms of chlorantraniliprole toxicity and detoxification in FAW, and will aid in monitoring the development of resistant strains for a newly pest to an old insecticide.
摘要：
Crystal toxins produced by different strains of entomopathogenic Bacillus thuringiensis (Bt) have been characterized and widely applied as commercial biological pesticides owing to their excellent insecticidal properties. This study aimed to identify novel bacterial strains effective in controlling Spodoptera exigua Hübner, Helicoverpa armigera Hübner, and Spodoptera litura Fabricius. Fifteen culturable bacterial strains were isolated from 60 dead larvae (H. armigera and S. exigua) collected in the field. The biochemical characteristics and 16S rRNA sequences of these strains indicated that one strain (B7) was Lysinibacillus sp., 12 strains (B1, B3, B4, B5, B6, B8, P2, P3, P4, P5, P6, and DW) were Bt kurstaki, and P2-2 and B2 were Bacillus velezensis subsp. Laboratory bioassays indicated that strains B3, P6, B6, and P4 showed high toxicity to second-instar larvae of S. exigua, with LC50 values of 5.11, 6.74, 205.82, and 595.93 µg/ml, respectively; while the strains P5, B5, B6, and P6, were the most efficient against second-instar larvae of H. armigera with LC50 values of 725.82, 11,022.72, 1,282.90, 2,005.28, respectively, and strains DW, P3, P2, and B4 had high insecticidal activity against second-instar larvae of S. litura with LC50 values of 576.69, 1,660.96, 6,309.42, and 5,486.10 µg/ml, respectively. In conclusion, several Bt kurstaki strains with good toxicity potential were isolated and identified in this study. These strains are expected to be useful for biointensive integrated pest management programs to reduce the use of synthetic insecticides.
通讯机构：
[Han, HL ] N;Northeast Forestry Univ, Sch Forestry, Harbin 150040, Peoples R China.;Northeast Forestry Univ, Northeast Asia Biodivers Res Ctr, Harbin 150040, Peoples R China.;Northeast Forestry Univ, Key Lab Sustainable Forest Ecosyst Management, Minist Educ, Harbin 150040, Peoples R China.
摘要：
The Genus Betapsestis was established by Matsumura in 1921, based on B. takeuchii from Honshu in Japan. It belongs to the subfamily Thyatirinae in the family Drepanidae, which is distributed in the Palaearctic and Oriental regions (Laszlo et al. 2007; Matsumura 1921). Only two species, B. umbrosa (Wileman, 1911) and B. brevis (Leech, 1900), have been known to date. Among them, B. brevis is endemic to China (Shaanxi, Sichuan, and Yunan), and B. umbrosa is endemic to Japan (Hokkaido, Honshu, Shikoku, and Kyushu), respectively (Laszlo et al. 2007; Matsumura 1921; Zhao 2004; Zhuang 2017). In this study, a new species of Betapsestis, B. shennongjiaensis Lai, Huang & Han sp. nov., from Shennongjia National Park in Hubei Province, China, is described as new to sciences.
期刊：
Journal of Cleaner Production,2023年421:138494 ISSN：0959-6526
通讯作者：
Zhang, Zhixiang;Xu, HH
作者机构：
[Wu, Hao; Zhang, Zhixiang; Miao, Xiaoran; Xu, HH; Hou, Ruiquan; Du, Pengrui; Wang, Ruifei; Xu, Hanhong; Li, Shengnan; Zhang, Ning] South China Agr Univ, Natl Key Lab Green Pesticide, Guangzhou 510642, Peoples R China.;[Hu, Wei; Zhang, Ning] Gannan Normal Univ, Coll Life Sci, Natl Navel Orange Engn Res Ctr, Ganzhou 341000, Peoples R China.;[Li, Youzhi] Hunan Agr Univ, Coll Plant Protect, Changsha 410000, Peoples R China.
通讯机构：
[Zhang, ZX; Xu, HH ] S;South China Agr Univ, Natl Key Lab Green Pesticide, Guangzhou 510642, Peoples R China.
关键词：
Nanopesticide;Controlled release;Antioxidant enzymes;Defense relative genes;Microbial community
摘要：
The frequent application of pesticides has caused adverse effects on humans and the environment. Stimuli responsive nanoparticles can enhance the control efficacy of pesticides to pest insects or fungus and reduce impacts on non-target organisms. In the present work, 4-(hydroxymethyl) phenylboronic acid pinacol ester (PBAP) groups were conjugated onto 13-cyclodextrin (13-CD) to form a H2O2-eliminating material (Ox-bCD), and then the nanoparticiles (PYR@OxbCD) loaded pyraclostrobin (PYR) were successfully fabricated by a nano precipitation. The prepared PYR@OxbCD had excellent reactive oxygen species (ROS) release dependency, and significantly enhanced fungicidal activity against Rhizoctonia solani in vitro. PYR@OxbCD could accurately release PYR at infected rice plants, providing a better control efficacy than PYR suspension concentrate (SC). The contact angle of PYR@OxbCD on leaves was reduced, and the spreading and antiphotolysis properties were increased, which may lead to an increase of deposition and a lower dissipation rate. Furthermore, the released PYR from PYR@OxbCD enhanced the activity of peroxidase (POD) and catalase (CAT), and up-regulated the expression of defense genes including PAL, PR10, CHI, CAT, GluPOD and AsPOD. PYR@OxbCD exhibited a much weaker effect on microbial abundance in rice cropping systems. The study provides an idea for smart delivery of fungicides to prevent plant pathogens and offers a more comprehensive view about the resistance of R. solani infected rice triggered by PYR@OxbCD in levels of phenotype, enzymatic activity, gene expression, and microbiome.
作者机构：
[Huang, Guo-Hua; Xiao, Zhengkun; Jin, Ruoheng] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Inse, Changsha, Peoples R China.;[Nakai, Madoka] Tokyo Univ Agr & Technol, Fuchu, Tokyo 1838509, Japan.;[Huang, Guo-Hua] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Inse, Changsha 410128, Hunan, Peoples R China.;[Nakai, Madoka] Tokyo Univ Agr & Technol, Dept Appl Biol Sci, Tokyo, Japan.
通讯机构：
[Madoka Nakai; Madoka Nakai Madoka Nakai Madoka Nakai] D;[Guo-Hua Huang; Guo-Hua Huang Guo-Hua Huang Guo-Hua Huang] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, People's Republic of China<&wdkj&>Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan
作者机构：
[Hou, Maolin; Zhong, Yuqi] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100093, Peoples R China.;[Zhong, Yuqi; Liao, Xiaolan] Hunan Agr Univ, Coll Plant Protect, Changsha 410028, Peoples R China.
通讯机构：
[Maolin Hou] S;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100093, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
Cyrtorhinus lividipennis;predation;reproduction;fitness;low temperature storage;biological control
摘要：
Simple Summary Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae) is an important predator of planthoppers and leafhoppers in rice fields. Augmentative biological control has been practiced successfully in many agroecosystems. However, one of the primary obstacles to augmentative biological control is obtaining natural enemies in sufficient numbers and quality when required for release. The development of the low-temperature storage (LTS) technique has been pivotal in ensuring the flexibility and efficiency of the mass production of biological control agents. Here, we measured the effects of LTS on the predatory capacity and reproduction of C. lividipennis adults and the fitness of the F1 generation. The results are expected to improve the successful utilization of the predator in an IPM program. Low-temperature storage (LTS) is a way to adjust natural enemy development to meet field release needs and to protect natural enemies from the odds of long-distance transportation. The mirid bug Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae) is an important predator of planthoppers and leafhoppers in rice fields. In this study, the LTS effects were measured on the predatory capacity and reproduction of the mirid adults (provided with 20% honey solution and stored at 13 degrees C for 12 days), and the fitness of the F1 generation of these adults. Higher predation of the eggs of the brown planthopper Nilaparvata lugens (Stal) (Hemiptera: Delphacidae) was observed in the post-storage females than in the control females. The functional responses of C. lividipennis adults, either exposed to LTS or not, to planthopper eggs fitted well with Holling type II functional responses. Longevity was not affected by LTS, whereas the number of offspring nymphs was 55.6% lower in the post-storage females than in the control females. The fitness of the offspring generation was not affected by the LTS of parental adults. The findings are discussed with their relevance to biological control.
摘要：
Ascoviruses are insect-specific viruses that are thought to utilize the cellular apoptotic processes of host larvae to produce numerous virion-containing vesicles. In this study, we monitored the in vivo infection processes of Heliothis virescens ascovirus 3h (HvAV-3h) to illustrate the regulated cell death (RCD) of host cells. Transmission electron microscopic observations did not reveal any morphological markers of apoptosis in the fat bodies or hemocytes of HvAV-3h-infected Helicoverpa armigera or Spodoptera exigua larvae. However, several hemocytes showed the morphological criteria for necrosis and/or pyroptosis. Further in vitro biochemical tests were performed to confirm the RCD type of host cells after infection with HvAV-3h. Different morphological characteristics were found between the early (prior to 24 hours post-infection, [hpi]) and later (48 to 120 hpi) stages in both HvAV-3h infected larval fat bodies and hemocytes. In the early stages, the virions could only be found in several adipohemocytes, and the fat bodies were cleaving their contained lipid inclusions into small lipid dots. In the later stage, both fat bodies and hemocytes were filled with numerous virions. According to the morphological characteristics of HvAV-3h infected larval fat bodies or hemocytes, the pathogenic characteristics and infection patterns of HvAV-3h in the host larvae were described, and the systematic pathogenic mode of ascovirus infection was refined in this study. This study details the complete infection process of ascoviruses, which provides insights into the relationship between a pathogenesis of an insect virus and the RCD of different host tissues at different stages of infection. IMPORTANCE Viruses and other pathogens can interrupt host cellular apoptosis to gain benefits, such as sufficient resources and a stable environment that enables them to complete their replication and assembly. It is unusual for viruses to code proteins with homology to caspases, which are commonly recognized as apoptosis regulators. Ascoviruses are insect viruses with special cytopathology, and they have been hypothesized to induce apoptosis in their host larvae via coding a caspase-like protein. This enables them to utilize the process of cellular apoptosis to facilitate vesicle formation and replication. However, our previous studies revealed different trends. The fat bodies and hemocytes of Heliothis virescens ascovirus 3h (HvAV-3h)-infected larvae did not show any morphological markers of apoptosis but did display necrosis and/or pyroptosis morphological characteristics. The pathogenic characteristics and infection patterns of HvAV-3h in the host larvae were described, which can help us understand the relationship between the pathogenesis of an insect virus and host RCD.
通讯作者：
Wei Li<&wdkj&>Liangying Dai<&wdkj&>Shiming Liu
作者机构：
[Cheng, Rong; Yi, Tuyong; Liu, Shiming; Li, Wei; Zheng, Xiang; Zhong, Chanjuan; Dai, Liangying] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Inse, Changsha 410128, Peoples R China.;[Liu, Shiming; Zhao, Jie] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.;[Liu, Tyler H.] Univ Wisconsin, Coll Letters & Sci, Madison, WI 53706 USA.;[Zhu, Zhendong] Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China.;[Xu, Jieting] Wimi Biotechnol Co Ltd, Changzhou 213000, Peoples R China.
通讯机构：
[Wei Li; Liangying Dai; Shiming Liu] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University , Changsha 410128 , P. R. China<&wdkj&>Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University , Changsha 410128 , P. R. China<&wdkj&>State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing 100193 , P. R. China
摘要：
Phytophthora sojae causes Phytophthora root and stem rot disease of soybean (Glycine max), leading to huge annual yield loss worldwide, but resistance to Phytophthora sojae (Rps) genes remains elusive. Soybean cultivar "Yudou 29" is resistant to P. sojae strain PsMC1, and this study aimed to clone, identify, and characterize the Rps gene in Yudou 29 (RpsYD29) and clarify its functional mechanism. We map-based cloned RpsYD29 (ZINC FINGER PROTEIN03, GmZFP03) using the families of a cross between Yudou 29 and a P. sojae-susceptible soybean cultivar "Jikedou 2". P. sojae resistance of GmZFP03 was functionally validated by stable soybean genetic transformation and allele-phenotype association analysis. GmZFP03 was identified as a C2H2-type zinc finger protein transcription factor, showing 4 amino acid residue polymorphisms (V79F, G122-, G123-, and D125V) and remarkably different expression patterns between resistant and susceptible soybeans. Notably boosted activity and gene expression of superoxide dismutase (SOD) in resistant-type GmZFP03-expressed transgenic soybean, substantial enhancement of P. sojae resistance of wild-type soybean by exogenous SOD treatment, and GmZFP03 binding to and activation of 2 SOD1 (Glyma.03g242900 and Glyma.19g240400) promoters demonstrated the involvement of SOD1s in GmZFP03-mediated resistance to P. sojae strain PsMC1. Thus, this study cloned the soybean P. sojae-resistant GmZFP03, the product of which specifically targets 2 SOD1 promoters. GmZFP03 can be directly used for precise P. sojae-resistance soybean breeding. The soybean transcription factor ZINC FINGER PROTEIN03 targets and activates two SUPEROXIDE DISMUTASE1 promoters to confer resistance to Phytophthora sojae.
通讯作者：
Zhenchuan Mao<&wdkj&>Yunsheng Wang<&wdkj&>Bingyan Xie<&wdkj&>Zhenchuan Mao Zhenchuan Mao Zhenchuan Mao<&wdkj&>Yunsheng Wang Yunsheng Wang Yunsheng Wang<&wdkj&>Bingyan Xie Bingyan Xie Bingyan Xie
作者机构：
[Mao, Zhenchuan; Jiao, Yang; Li, Yan; Xie, Bingyan; Ling, Jian; Yang, Yuhong; Jiang, Lijun; Zhao, Jianlong] Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing, Peoples R China.;[Yang, Yu] Shandong Acad Agr Sci, Inst Vegetables, Jinan, Shandong, Peoples R China.;[Yang, Yu] Shandong Acad Agr Sci, Inst Modern Agr Yellow River Delta, Jinan, Shandong, Peoples R China.;[Wang, Yunsheng] Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.
通讯机构：
[Zhenchuan Mao; Bingyan Xie; Zhenchuan Mao Zhenchuan Mao Zhenchuan Mao; Bingyan Xie Bingyan Xie Bingyan Xie] S;[Yunsheng Wang; Yunsheng Wang Yunsheng Wang Yunsheng Wang] C;College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China
摘要：
Root-knot nematodes (RKNs) are infamous plant pathogens in tomato production, causing considerable losses in agriculture worldwide. Mi-1 is the only commercially available RKN-resistance gene; however, the resistance is inactivated when the soil temperature is over 28 °C. Mi-9 in wild tomato (Solanum arcanum LA2157) has stable resistance to RKNs under high temperature but has not been cloned and applied. In this study, a chromosome-scale genome assembly of S. arcanum LA2157 was constructed through Nanopore and Hi-C sequencing. Based on molecular markers of Mi-9 and comparative genomic analysis, the localization region and candidate Mi-9 genes cluster consisting of seven nucleotide-binding sites and leucine-rich repeat (NBS-LRR) genes were located. Transcriptional expression profiles confirmed that five of the seven candidate genes were expressed in root tissue. Moreover, virus-induced gene silencing of the Sarc_034200 gene resulted in increased susceptibility of S. arcanum LA2157 to Meloidogyne incognita, and genetic transformation of the Sarc_034200 gene in susceptible Solanum pimpinellifolium conferred significant resistance to M. incognita at 25 °C and 30 °C and showed hypersensitive responses at nematode infection sites. This suggested that Sarc_034200 is the Mi-9 gene. In summary, we cloned, confirmed and applied the heat-stable RKN-resistance gene Mi-9, which is of great significance to tomato breeding for nematode resistance.
通讯机构：
[Guo-Hua Huang] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan 410128, People's Republic of China<&wdkj&>Institute of Virology, Hunan Agricultural University, Changsha, Hunan 410128, People's Republic of China
摘要：
In insects, lipids and trehalose are the primary energy reserves and sources, and their metabolism is controlled by a variety of physiological processes. Adipokinetic hormone receptor (AKHR) has been reported to be involved in glycolipid metabolism and immune response, however, is an uncharted territory in Helicoverpa armigera. In the present study, the full-length cDNA encoding AKHR was isolated from H. armigera. The HaAKHR contains a seven-transmembrane domain, which was a characteristic of G-protein coupled receptors. The expression profile analysis revealed that HaAKHR was highly expressed in fat body of newly emerged female adults and highly expressed in the immature stage. The content of triglyceride (TAG) in fat body increased significantly after silencing of HaAKHR, however, the trehalase activity and the expression of trehalose-6-phosphate synthase (TPS) were decreased in hemolymph. Furthermore, the expression level of HaAKHR was significantly upregulated when infected with different kinds of entomopathogenic microbes. The above results suggested that HaAKHR plays an important role in regulating glycolipid metabolism and immune response in H. armigera, and contribute to understanding the underlying mechanism of host-pathogen interactions in H. armigera.
摘要：
Rhizoctonia solani is a widespread and devastating plant pathogenic fungus that infects many important crops. This pathogen causes tobacco target spot, a disease that is widespread in many tobacco-growing countries and is destructive to tobacco. To identify antagonistic microorganisms with biocontrol potential against this disease, we isolated Streptomyces strains from forest inter-root soil and screened a promising biocontrol strain, ZZ-21. Based on in vitro antagonism assays, ZZ-21 showed a significant inhibitory effect on R. solani and various other phytopathogens. ZZ-21 was identified as Streptomyces olivoreticuli by its phenotypic, genetic, physiological and biochemical properties. Complete genome sequencing revealed that ZZ-21 harbored numerous antimicrobial biosynthesis gene clusters. ZZ-21 significantly reduced the lesion length in detached inoculated leaf assays and reduced the disease index under greenhouse and field conditions. Based on an in vitro antagonistic assay of ZZ-21 culture, the strain exhibited an antifungal activity against R. solani in a dose-dependent manner. The culture filtrate could impair membrane integrity, possibly through membrane lipid peroxidation. ZZ-21 could secrete multiple extracellular enzymes and siderophores. According to a series of antifungal assays, the extracellular metabolites of ZZ-21 contained antimicrobial bioactive compounds composed of proteins/peptides extracted using ammonium sulfate precipitation, which were stable under stress caused by high temperature and protease K. The EC(50) value for ammonium sulfate precipitation was determined to be 21.11μg/mL in this study. Moreover, the proteins/peptides also exhibited biocontrol ability and were observed to alter the plasma membrane integrity of R. solani which were evaluated by biocontrol efficacy assays on detached tobacco leaves and PI staining. Overall, strain ZZ-21 shows the potential to be developed into a biopesticide against tobacco target spot disease.
摘要：
As a subtropical and tropical tree, bayberry (Myrica rubra) is an important fruit tree grown commercially in southern China. Interestingly, our studies found that the fruit quality of bayberry with accompanying ryegrass was significantly improved, but its mechanism remains unclear. The aim of this study was to explore the mechanism of accompanying ryegrass on the beneficial effect of the fruit quality of bayberry by measuring the vegetative growth parameters, fruit parameters with economic impact, physical and chemical properties of rhizosphere soil, microbial community structure, and metabolites of the bayberry with/without ryegrass. Notably, the results revealed a significant difference between bayberry trees with and without accompanying ryegrass in fruit quality parameters, soil physical and chemical properties, microbial community structure, and metabolites. Compared with the control without accompanying ryegrass, the planting of ryegrass increased the titratable sugar, vitamin C, and titratable flavonoid contents of bayberry fruits by 2.26%, 28.45%, and 25.00%, respectively, and decreased the titratable acid contents by 9.04%. Furthermore, based on 16S and ITS amplicon sequencing of soil microflora, the accompanying ryegrass caused a 12.47% increment in Acidobacteriota while a 30.04% reduction in Actinobacteria was recorded, respectively, when compared with the bayberry trees without ryegrass. Redundancy discriminant analysis of microbial communities and soil properties indicated that the main variables of the bacterial community included available nitrogen, available phosphorus, exchangeable aluminum, and available kalium, while the main variables of the fungal community included exchangeable aluminum, available phosphorus, available kalium, and pH. In addition, the change in microbial community structure was justified by the high correlation analysis between microorganisms and secondary metabolites. Indeed, GC-MS metabolomics analysis showed that planting ryegrass caused a 3.83%–144.36% increase in 19 metabolites such as 1,3-Dipentyl-heptabarbital and carbonic acid 1, respectively, and a 23.78%–51.79% reduction of 5 metabolites compared to the bayberry trees without the accompanying ryegrass. Overall, the results revealed the significant change caused by the planting of ryegrass in the physical and chemical properties, microbiota, and secondary metabolites of the bayberry rhizosphere soils, which provides a new insight for the ecological improvement of bayberry.
作者机构：
[Huang, Guo-hua; Lv, Jun-xian] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Inse, Changsha 410128, Hunan, Peoples R China.;[Wang, X. I. N. G.] Qiongtai Normal Univ, Coll Sci, Trop Biodivers & Bioresource Utilizat Lab, Haikou 571100, Hainan, Peoples R China.
关键词：
New record;Female genitalia;Neopseustidae;Yintiaoling Nature Reserve
摘要：
In 2022, the insect inventories organized by Prof. Zhi-Sheng Zhang of Southwest University were constructed in Yintiaoling Nature Reserve, Chongqing Municipality, China. The neopseustid moth from Chongqing based on three specimens of Neopseustis archiphenax by light trapping were reported in this paper. The photos of the male and female adults, genitalia, and abdominal special structures are presented with the female described firstly. Also, the DNA barcoding sequence data is provided, and the key to the Neopseustis species is given.
