摘要：
为探讨同一时间内黄心夜合Michelia martinii鲜叶、鲜嫩枝和鲜花的挥发油成分,利用气相色谱-质谱联用技术(GC-MS)对水蒸气蒸馏法提取的黄心夜合鲜叶、鲜嫩枝和鲜花挥发油成分进行了分析。结果表明:去掉峰值0.49以下的峰,从黄心夜合鲜叶、鲜嫩枝、鲜花分别检测出21种、31种和25种成分, 3种不同组织样品共有2,4-二异丙烯基-1-甲基环己烷、γ-依兰油烯、4(14),11-桉叶二烯、γ-榄香烯等4种成分,而鲜叶与鲜嫩枝都释放共同的12种挥发性成分,鲜嫩枝与鲜花都释放共同的13种挥发性成分,鲜叶与鲜花都释放共同的4种挥发性成分。黄心夜合鲜叶、鲜嫩枝和鲜花的挥发油成分在提取材料选择、挥发性成分利用与养生保健群落的营建等方面具有一定的应用参考。 <&wdkj&>To study volatile components from fresh leaves, fresh twigs, and fresh flowers of Michelia martinii at the same time, volatile oils were extracted by water-steam distillation from the fresh leaves, fresh twigs, and fresh flowers and then detected by gas chromatography-mass spectrometry (GC-MS). Results of GC-MS showed that the volatile oils contents from different organizations of M. martinii at the same time had ingredients of 21 kinds from fresh leaves, of 31 kinds from fresh twigs, and of 25 kinds from fresh flowers (peak values below 0.49 were neglected). Among the three fresh organizations of M. martinii, the common components were four kinds of ingredients, such as .gamma.-Elemene, gamma.-Muurolen, Eudesma-4(14),11-diene, and Cyclohexane, 2,4-diisopropenyl-1-methyl-. Four kinds of components were common to fresh leaves and fresh flowers, 13 kinds of components were common to fresh flowers and fresh twigs, and 12 kinds of components were common to fresh leaves and fresh twigs. The volatile oils of flowers were rich in terpenes and alcohols with terpenes dominated by monoterpenes up to 36.38%; content of the alcohol compounds was higher than terpenes with content as high as 54.77%. Terpene compounds had .gamma.-Cadinene, Caryophyllene oxide, .alpha.-Selinene, Spathulen, Diepicedrene-1-oxide, (+)-Ledene, -Aristolene, Eudesma-4 (14),11-diene, and DGermacrene D. While the alcohols had Eudesm-4 (14)-en-11-,2-(4,8-Dimethyl-3,7-cyclodecadien-1-yl)-2-p, Epiglobulol,.gamma.-Eudesmol, .tau.-Cadinol, Guaiol, and .delta.-Cadinol. In conclusion M. mar tinii could provide new application ideas on material selection of extracted essential oils, utilization of volatile components, and landscape community construction for health care.
作者：
Lei Ling-hua*;Hou Jing;Liu Hai-yan;Chen Yan-hua;Yu Xiao-ying*;...
期刊：
PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES,2019年32(1):383-389 ISSN：1011-601X
通讯作者：
Lei Ling-hua;Yu Xiao-ying
作者机构：
[Liu Hai-yan; Lei Ling-hua; Guo Zhi-ping; Hou Jing] Lishui Univ, Coll Ecol, Lishui, Zhejiang, Peoples R China.;[Lei Ling-hua] Key Lab Hunan Prov Study & Utilizat Ethn Med Plan, Huaihua, Hunan, Peoples R China.;[Yu Xiao-ying; Chen Yan-hua] Hunan Agr Univ, Coll Hort & Landscape, Changsha, Hunan, Peoples R China.;[Chen Yan-hua] Hunan Inst Sci & Technol, Yueyang, Hunan, Peoples R China.
通讯机构：
[Lei Ling-hua] L;[Lei Ling-hua] K;[Yu Xiao-ying] H;Lishui Univ, Coll Ecol, Lishui, Zhejiang, Peoples R China.;Key Lab Hunan Prov Study & Utilizat Ethn Med Plan, Huaihua, Hunan, Peoples R China.
关键词：
Hyperlipidemia activity;antioxidant;volatile oils;Michelia martini Levl
摘要：
.This study aimed to investigate the hypolipidemic and antioxidant activities of volatile oils from Michelia martini Levl. The antioxidant property of volatile oils from Michelia martini in vitro was investigated by establishment of various systems. High fat diet induced rats were used to assess the hypolipidemic and antioxidant activities of Michelia martini volatile oils in vivo. The level of total cholesterol, triglycerides, high density lipoprotein cholesterol, low density lipoprotein cholesterol, alanine transaminase, aspartate aminotransferase, alkaline phosphatase and gamma-glutamyl transpeptidase in serum, and the activities of catalase, malondialdehyde, super oxide dismutase and glutathione in liver of rats were assayed by standard procedures. Our results showed that Michelia martini exhibits strong hypolipidemic and antioxidant activities both in vitro and vivo. Our data were also supplemented with histopathological studies on liver tissues and aorta sections of rats.
摘要：
Background: An increasing number of plant components and their extracts have been shown to have beneficial health effects in humans. We aimed to explore the antioxidant effects of the aqueous extract of Asparagus cochinchinensis (Lour.) Men. shoot in vivo and in vitro. Methods: A total of 80 Kun Ming mice were randomly divided into four groups (20/group). The mice in the control group received a daily subcutaneous injection of saline. A daily injection of D-galactose was administered to the aging model group, the vitamin C (Vc) group (positive control group), and the extract treatment group. Regular measurement of blood cells, nitric oxide synthase (NOS), catalase (CAT) activities, superoxide dismutase (SOD) activities, nitric oxide (NO), and malondialdehyde (MDA) concentration, and the expressions of NOS, SOD, and glutathione peroxidase (GPX) in serum levels were obtained. Furthermore, the microstructure of mice viscera was observed using hematoxylin and eosin staining. Results: The aqueous extract of A. cochinchinensis (Lour.) Men. had similar 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH center dot) [or 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)] and higher hydroxyl radicals (or superoxide anion; p < 0.05) radical scavenging capabilities to Vc. Moreover, compared with the aging model group, the aqueous extract of A. cochinchinensis (Lour.) Men. shoot could obviously increase NOS, CAT, and SOD activities and the NO content, and reduce the MDA content (p < 0.05). Additionally, the microstructure of mice viscera was obviously improved and the expressions of NOS, SOD and GPX were also manifestly increased in the treatment group (p < 0.05). Conclusion: The aqueous extract of A. cochinchinensis (Lour.) Men. shoot had a strong radical scavenging capability in vivo and in vitro, and might be used to diminish radicals in the body and consequently prevent aging. Copyright (C) 2016, the Chinese Medical Association. Published by Elsevier Taiwan LLC.
