作者机构：
[李旭; 侯志勇; 谢永宏; Li, Xu; Zeng, Jing] Key Laboratory of Agro-ecological Processes in Subtropical Region, Dongting Lake Station for Wetland Ecosystem Research, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha;410125, China;[于晓英] College of Horticulture, Hunan Agricultural University, Changsha;410128, China;[李旭] 410125, China <&wdkj&> College of Horticulture, Hunan Agricultural University, Changsha
摘要：
为探讨同一时间内黄心夜合Michelia martinii鲜叶、鲜嫩枝和鲜花的挥发油成分,利用气相色谱-质谱联用技术(GC-MS)对水蒸气蒸馏法提取的黄心夜合鲜叶、鲜嫩枝和鲜花挥发油成分进行了分析。结果表明:去掉峰值0.49以下的峰,从黄心夜合鲜叶、鲜嫩枝、鲜花分别检测出21种、31种和25种成分, 3种不同组织样品共有2,4-二异丙烯基-1-甲基环己烷、γ-依兰油烯、4(14),11-桉叶二烯、γ-榄香烯等4种成分,而鲜叶与鲜嫩枝都释放共同的12种挥发性成分,鲜嫩枝与鲜花都释放共同的13种挥发性成分,鲜叶与鲜花都释放共同的4种挥发性成分。黄心夜合鲜叶、鲜嫩枝和鲜花的挥发油成分在提取材料选择、挥发性成分利用与养生保健群落的营建等方面具有一定的应用参考。 <&wdkj&>To study volatile components from fresh leaves, fresh twigs, and fresh flowers of Michelia martinii at the same time, volatile oils were extracted by water-steam distillation from the fresh leaves, fresh twigs, and fresh flowers and then detected by gas chromatography-mass spectrometry (GC-MS). Results of GC-MS showed that the volatile oils contents from different organizations of M. martinii at the same time had ingredients of 21 kinds from fresh leaves, of 31 kinds from fresh twigs, and of 25 kinds from fresh flowers (peak values below 0.49 were neglected). Among the three fresh organizations of M. martinii, the common components were four kinds of ingredients, such as .gamma.-Elemene, gamma.-Muurolen, Eudesma-4(14),11-diene, and Cyclohexane, 2,4-diisopropenyl-1-methyl-. Four kinds of components were common to fresh leaves and fresh flowers, 13 kinds of components were common to fresh flowers and fresh twigs, and 12 kinds of components were common to fresh leaves and fresh twigs. The volatile oils of flowers were rich in terpenes and alcohols with terpenes dominated by monoterpenes up to 36.38%; content of the alcohol compounds was higher than terpenes with content as high as 54.77%. Terpene compounds had .gamma.-Cadinene, Caryophyllene oxide, .alpha.-Selinene, Spathulen, Diepicedrene-1-oxide, (+)-Ledene, -Aristolene, Eudesma-4 (14),11-diene, and DGermacrene D. While the alcohols had Eudesm-4 (14)-en-11-,2-(4,8-Dimethyl-3,7-cyclodecadien-1-yl)-2-p, Epiglobulol,.gamma.-Eudesmol, .tau.-Cadinol, Guaiol, and .delta.-Cadinol. In conclusion M. mar tinii could provide new application ideas on material selection of extracted essential oils, utilization of volatile components, and landscape community construction for health care.
摘要：
.This study aimed to investigate the hypolipidemic and antioxidant activities of volatile oils from Michelia martini Levl. The antioxidant property of volatile oils from Michelia martini in vitro was investigated by establishment of various systems. High fat diet induced rats were used to assess the hypolipidemic and antioxidant activities of Michelia martini volatile oils in vivo. The level of total cholesterol, triglycerides, high density lipoprotein cholesterol, low density lipoprotein cholesterol, alanine transaminase, aspartate aminotransferase, alkaline phosphatase and gamma-glutamyl transpeptidase in serum, and the activities of catalase, malondialdehyde, super oxide dismutase and glutathione in liver of rats were assayed by standard procedures. Our results showed that Michelia martini exhibits strong hypolipidemic and antioxidant activities both in vitro and vivo. Our data were also supplemented with histopathological studies on liver tissues and aorta sections of rats.
关键词：
Callus;Hydropriming;Paclitaxel;Taxoid-metabolite;Taxus chinensis var. mairei;Tube plantlet
摘要：
The half-sib population is an abundant of gene pool for selection breeding of lines or cultivars with high paclitaxel. The seeds, collected from the sampling tree of Taxus chinensis var. mairei, were exposed to hydropriming and incubated plantlets and which induced callus to screen stable and high paclitaxel content of cell lines. Seed dormancy was successfully broken with 100% germination rate by hydropriming for three to five days. Embryo germination was inhibited by illumination. The optimal medium for in vitro embryo germination was MS-modified media (BLG media) supplemented with 20 g/L sucrose, 5 g/L activated carbon and 7 g/L agar. The embryos were transferred to a 14 h photoperiod after seven days incubation in darkness. Seedling plantlets were obtained after fifty days in vitro. Embryonic calluses were induced with frequencies up to 73% and cultured in BLG medium supplemented with 2 mg/L 2,4-dichlorophenoxyacetic-D (2,4-D), 0.5 mg/L 6-benzyladenine (6-BA), 20 g/L sucrose and 7 g/L agar. The half-sib cell lines showed high variation levels of taxoids metabolite. Thus, a cell line with high paclitaxel content [60.87 mg/100 g fresh weight] was obtained. Our study provides a new strategy to achieve high seed germination rate and seedling plantlet growth of T. chinensis var. mairei. It is suitable for screening cell lines with high paclitaxel content, obtained from half-sib cells that initiated from the seedling plantlets of T. chinensis var. mairei. (C) 2019 Friends Science Publishers
