作者机构:
[黄海涛] College of Resource and Environment, Hunan Agricultural University, Changsha 410128, China;National Engineering Laboratory of High Utilization Efficiency of Soil Fertilizer Resource, Changsha 410128, China;[官春云] National Center of Oilseed Crops Improvement, Hunan Branch, Changsha 410128, China;[彭建伟; 张振华; 荣湘民; 刘强; 宋海星] College of Resource and Environment, Hunan Agricultural University, Changsha 410128, China, National Engineering Laboratory of High Utilization Efficiency of Soil Fertilizer Resource, Changsha 410128, China
通讯机构:
[Huang, H.-T.] C;College of Resource and Environment, Hunan Agricultural University, China
摘要:
Combined application of synthetic nitrogen (N) fertilizers and organic materials can enhance soil quality, but little is known about the distribution of fertilizer N among different soil fractions after crop harvest. A pot experiment using 15N tracer was employed to address this question with three treatments, i.e., labeled urea-only (15NU), labeled urea + rice straw (15NU-S) and labeled rice straw + urea (15NS-U) applied to a Ferallic Cambisol (1:1 type soil clay mineral) and a Calcaric Fluvisol (2:1 clay mineral). Soil microbial biomass N, fixed ammonium (fixed NH
4
+
), exchangeable ammonium and soil organic N fractions by hydrolysis (6 N HCl) and their isotope abundance were determined after the rice harvest. Soil newly formed N in urea + straw (U-S) treatments (15NU-S, 15NS-U) was the sum of labeled urea-N in 15NU-S and labeled straw-N in 15NS-U. Compared with 15NU, U-S significantly (P < 0.05) increased the content and percentage of newly formed total soil N, acid insoluble N, amino acid N, and hydrolysable unknown N in both soils. In U-S treatment, straw amendment significantly (P < 0.05) reduced the content and percentage of newly formed fixed-NH
4
+
-N in Fluvisol as compared with 15NU treatments. Soil microbes contributed to the larger percentage of newly formed amino acid N (P < 0.01) in Cambisol as compared with Fluvisol. Fertilizer N in various soil fractions was therefore strongly affected by clay mineral type and microbes after the combined application of organic materials and synthetic N fertilizer.
作者机构:
[Zheng Xiaodong; Zhang Zhenhua; Rong Xiangmin; Luo Zunchang; Liu Qiang; Song Haixing; Peng Jianwei; Xie Guixian; Zhang Yuping; Gu Jidong] College of Resources and Environment,Hunan Agricultural University;[Zheng Xiaodong; Zhang Zhenhua; Rong Xiangmin; Luo Zunchang; Liu Qiang; Song Haixing; Peng Jianwei; Xie Guixian; Zhang Yuping; Gu Jidong] Soil and Fertilizer Institute of Hunan Province;[Zheng Xiaodong; Zhang Zhenhua; Rong Xiangmin; Luo Zunchang; Liu Qiang; Song Haixing; Peng Jianwei; Xie Guixian; Zhang Yuping; Gu Jidong] Hunan Provincial Key Lab. Plant Nutrition Common University;[Zheng Xiaodong; Zhang Zhenhua; Rong Xiangmin; Luo Zunchang; Liu Qiang; Song Haixing; Peng Jianwei; Xie Guixian; Zhang Yuping; Gu Jidong] Hunan Provincial Key Lab. Farmland Pollution Control Agri. Resources Use;[Zheng Xiaodong; Zhang Zhenhua; Rong Xiangmin; Luo Zunchang; Liu Qiang; Song Haixing; Peng Jianwei; Xie Guixian; Zhang Yuping; Gu Jidong] School of Biological Sciences,The University of Hong Kong
会议名称:
Environmental Pollution and Public Health(EPPH 2012)
会议时间:
2012-05-17
会议地点:
中国上海
摘要:
In order to study the research development of different remediation methods and the future works of heavy metal pollution, we will give a brief summary of current situation of soil heavy metal polluti
期刊:
Australian Journal of Crop Science,2012年6(9):1377-1382 ISSN:1835-2693
通讯作者:
Song, H.-X.
作者机构:
College of Agricultural Resources and Environment, Hunan Agricultural University, Changsha, China;Hunan Provincial Key Laboratory of Plant Nutrition in Common University, Changsha, 410128, China;Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use, Changsha, 410128, China;National Engineering Laboratory of High Utilization Efficiency of Soil Fertilizer Resource, Changsha, 410128, China;National Center of Oilseed Crops Improvement Hunan Branch, Changsha, 410128, China
通讯机构:
College of Agricultural Resources and Environment, Hunan Agricultural University, China
关键词:
Nitrate nitrogen;Nitrogen use efficiency;Proton pump of tonoplast
摘要:
Large amount of N03 --N are accumulated in vacuole, and cannot be timeously reducted, reutilized and transported into cytoplasm. It is the main reason for great N03--N accumulation in vacuole and nitrogen (N) use efficiency cannot be further improved. Transport mechanism of N03--N across tonoplast is explained in this paper, there are two proton pumps (H+-ATPase and H+-PPase) on tonoplast with absolutely different biology functions and physical characteristic. Mg·ATP and Mg·PPi are the specific substrates of H+-ATPase and H+-PPase respectively, hydrolysis H+ is pumped into vacuole, and contribution to build electrochemical proton gradient between cytoplasm and vacuole. N03--N transport from vacuole to cytoplasm greatly depends on electrochemical proton gradient, N03--N transport from cytoplasm to vacuole is mainly achieved by vacuole H+/N03-antiport system, while symport system (vacuole N03--N combined with anion) is of benefit for vacuole N03--N transporting into cytoplasm. N03--N transported by proton pump of tonoplast is influenced by NR activity in cytoplasm, N03--N can be continuing assimilation and reduction by NR in cytoplasm, and accelerating vacuole N03--N transported into cytoplasm. These results will supply references and research forecast for further study on efficiency and practicable methods of N utilization, and improving reuse efficiency of N03--N in plant tissues.