Effects of redox potential on soil cadmium solubility:Insight into microbial community
作者：
Meng, Delong;Li, Juan;Liu, Tianbo;Liu, Yongjun;Yan, Mingli;...
期刊：
环境科学学报(英文版) ,2019年75(01):224-232 ISSN：1001-0742
通讯作者：
Yin, Huaqun
作者机构：
[Li, Xiaoqi; Liu, Hongwei; Meng, Delong; Liu, Xueduan; Yin, Huaqun; Liang, Yili; Hu, Jin] Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.;[Li, Juan; Liu, Yongjun] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Liu, Tianbo] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Li, Xiaoqi; Liu, Hongwei; Meng, Delong; Liu, Xueduan; Yin, Huaqun; Liang, Yili; Hu, Jin] Minist Educ, Key Lab Biomet, Changsha 410083, Peoples R China.;[Yan, Mingli] Hunan Univ Sci & Technol, Sch Life Sci, Xiangtan 411201, Peoples R China.
通讯机构：
[Yin, Huaqun] C;[Yin, Huaqun] M;Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.;Minist Educ, Key Lab Biomet, Changsha 410083, Peoples R China.
关键词：
Cd solubility;Redox potential;Microbial community;Molecular ecology networks (MENs);Anaerolineaceae
摘要：
Understanding the role of microbes in the solubility of cadmium (Cd) is of fundamental importance for remediation of Cd toxicity. The present study aimed to identify the microbes that involved in regulating Cd solubility and to reveal possible mechanisms. Therefore, microbial communities were investigated through high-throughput sequencing approach, the molecular ecological network was constructed and metagenomes were predicted. Our results indicated that redox conditions affected both the solubility of soil Cd and the microbial communities. Anaerobic microbes, such as Anaerolineaceae, did not only play important roles in shaping the microbial community in soils, but might also be involved in regulating the Cd solubility. Two possible mechanisms that how Anaerolineaceae involved in Cd solubility are (1) Anaerolineaceae are important organic matter degraders under anoxic conditions and (2) Anaerolineaceae can co-exist with methane metabolism microbes, while methane metabolism promotes the precipitation of soluble Cd. Thus, application of Anaerolineaceae in bioremediation of soil Cadmium contamination is a potential approach. The study provided a novel insight into the role of microbial community in the regulation of Cd solubility under different redox conditions, and suggested a potential approach for the remediation of soil Cd contamination. (c) 2018 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
语种：
英文
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Study on the growth and spectral response of rice with different nitrogen morphologies in water-saving irrigation model
作者：
Xiao Zhifang;Fang Xilin;Wang Yue L;Zeng Rongliang;Li Jianhui;...
期刊：
Proceedings of SPIE - The International Society for Optical Engineering ,2019年11023 ISSN：0277-786X
通讯作者：
Chen Guanghui
作者机构：
[Wang Yue L; Xiao Zhifang; Fang Xilin; Chen Guanghui] Hunan Agr Univ, Coll Agr, Changsha 410128, Hunan, Peoples R China.;[Li Jianhui; Zeng Rongliang] Hunan Zhong Ke Star Map Informat Technol Co LTD, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Chen Guanghui] H;Hunan Agr Univ, Coll Agr, Changsha 410128, Hunan, Peoples R China.
会议名称：
5th Symposium on Novel Optoelectronic Detection Technology and Application
会议时间：
OCT 24-26, 2018
会议地点：
Xian, PEOPLES R CHINA
会议主办单位：
[Xiao Zhifang;Fang Xilin;Wang Yue L;Chen Guanghui] Hunan Agr Univ, Coll Agr, Changsha 410128, Hunan, Peoples R China.^[Zeng Rongliang;Li Jianhui] Hunan Zhong Ke Star Map Informat Technol Co LTD, Changsha 410128, Hunan, Peoples R China.
会议论文集名称：
Proceedings of SPIE
关键词：
Hyper-spectral;Monitoring of chlorophyll content;Rice;Water-saving irrigation
摘要：
Hyper-spectral remote sensing is now a frontier of the remote sensing technology, which can monitoring chlorophyll content rapidly and nondestructive. In this study, four nitrogen forms were set up?1/4?ammonium sulfate (ammonium nitrogen) B1, potassium nitrate (nitrate nitrogen) B2, urea (amide nitrogen) B3, and formaldehyde urea (slow effect nitrogen fertilizer) B4. The chlorophyll content of B4 treated with different nitrogen forms was significantly different. Spectral parameters also show good correlation. The chlorophyll content estimation model constructed with the spectral parameters DVI(755,690) and DVI(810,690) has a good fitting degree, and the linear model R2 has reached a significant level, respectively. Rice treated with traditional urea had a lower growth rate than the other three types of nitrogen, the slow effect nitrogen fertilizer and ammonium nitrogen were suggested to improve nitrogen utilization efficiency and eventually reach the goal of high yield. © 2019 SPIE.
语种：
英文
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Genome-wide identification of loci affecting seed glucosinolate contents in Brassica napus L
作者：
Wei, Dayong;Cui, Yixin;Mei, Jiaqin;Qian, Lunwen;Lu, Kun;...
期刊：
Acta Botanica Sinica ,2019年61(5):611-623 ISSN：1672-6650
通讯作者：
Tang, Qinglin;Qian, Wei
作者机构：
[Wei, Dayong; Tang, Qinglin; Wang, Zhi-Min] Southwest Univ, Coll Hort & Landscape Architecture, Chongqing 400715, Peoples R China.;[Wei, Dayong; Tang, Qinglin; Wang, Zhi-Min] Minist Educ, Key Lab Hort Sci Southern Mt Reg, Chongqing 400715, Peoples R China.;[Wei, Dayong; Mei, Jiaqin; Li, Jiana; Lu, Kun; Cui, Yixin; Qian, Wei] Southwest Univ, Coll Agron & Biotechnol, Chongqing 400715, Peoples R China.;[Mei, Jiaqin; Li, Jiana; Lu, Kun; Cui, Yixin; Qian, Wei] Southwest Univ, Acad Agr Sci, Chongqing 400715, Peoples R China.;[Qian, Lunwen] Hunan Agr Univ, Collaborat Innovat Ctr Grain & Oil Crops South Ch, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Tang, Qinglin; Qian, Wei] S;[Tang, Qinglin] M;Southwest Univ, Coll Hort & Landscape Architecture, Chongqing 400715, Peoples R China.;Minist Educ, Key Lab Hort Sci Southern Mt Reg, Chongqing 400715, Peoples R China.;Southwest Univ, Coll Agron & Biotechnol, Chongqing 400715, Peoples R China.
关键词：
GLUCOSINOLATES;are;AMINO;defense;agents;against;group-specific;HB
摘要：
Glucosinolates are amino acid-derived secondary metabolites that act as chemical defense agents against pests. However, the presence of high levels of glucosinolates severely diminishes the nutritional value of seed meals made from rapeseed (Brassica napus L.). To identify the loci affecting seed glucosinolate content (SGC), we conducted genome-wide resequencing in a population of 307 diverse B. napus accessions from the three B. napus ecotype groups, namely, spring, winter, and semi-winter. These resequencing data were used for a genome-wide association study (GWAS) to identify the loci affecting SGC. In the three ecotype groups, four common and four ecotype-specific haplotype blocks (HBs) were significantly associated with SGC. To identify candidate genes controlling SGC, transcriptome analysis was carried out in 36 accessions showing extreme SGC values. Analyses of haplotypes, genomic variation, and candidate gene expression pointed to five and three candidate genes in the common and spring group-specific HBs, respectively. Our expression analyses demonstrated that additive effects of the three candidate genes in the spring group-specific HB play important roles in the SGC of B. napus. © 2018 Institute of Botany, Chinese Academy of Sciences
语种：
英文
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Pseudomonas species isolated from tobacco seed promote root growth and reduce lead contents in Nicotiana tobacum K326.
作者：
Li, Juan;Zheng, Bufan;Hu, Ruiwen;Liu, Yongjun;Jing, Yongfeng;...
期刊：
CANADIAN JOURNAL OF MICROBIOLOGY ,2019年65(3):214-223 ISSN：0008-4166
通讯作者：
Chen, Wu;Zhou, Qingming
作者机构：
[Zhou, Qingming; Zheng, Bufan; Hu, Ruiwen; Chen, Wu; Chen, W; Zhou, QM; Xiao, Yunhua; Sun, Min; Li, Juan] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Zhou, Qingming; Zheng, Bufan; Hu, Ruiwen; Chen, Wu; Chen, W; Zhou, QM; Xiao, Yunhua; Sun, Min; Li, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Zhou, Qingming; Zheng, Bufan; Hu, Ruiwen; Chen, Wu; Chen, W; Zhou, QM; Xiao, Yunhua; Sun, Min; Li, Juan] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Liu, Yongjun] Inst Hunan Prov Tobacco Sci Res, Changsha 410004, Hunan, Peoples R China.;[Jing, Yongfeng] China Tobacco Hunan Ind Co Ltd, Changsha 410019, Hunan, Peoples R China.
通讯机构：
[Chen, W; Zhou, QM] H;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.
关键词：
captation du plomb;croissance racinaire;endophytes;lead uptake;root growth
摘要：
Endophytic bacteria are generally helpful for plant growth and protection. We isolated from tobacco seeds three Pseudomonas strains (K03, Y04, and N05) that could produce siderophores, indole-3-acetic acid, and 1-aminocyclopropane-1-carboxylate deaminase, fix nitrogen, dissolve phosphorus and potassium, and tolerate heavy metals. In pot experiments, the three isolated strains significantly promoted root growth and increased the root enzyme activity in Nicotiana tobacum K326. Furthermore, bacterial inoculations increased the proportion of residual lead (Pb) by 8.36%–51.63% and decreased the total Pb content by 3.28%–6.38% in the contaminated soil during tobacco planting, compared with uninoculated soils. An effective decrease in Pb content was also found in tobacco leaves with bacterial inoculations. K03 inoculation decreased the Pb content in the upper leaves by 49.80%, and Y04 inoculation had the best effect, decreasing the Pb content in the middle leaves by 70.12%. Additionally, soil pH and root activity had significant effects on transformation and translocation of Pb. The study suggested that in response to Pb pollution in soil, a reasonable application of endophytes (e.g., Pseudomonas) might be a promising approach in promoting tobacco growth and reducing Pb content in tobacco, while simultaneously enhancing Pb stabilization in soils. © 2019, Published by NRC Research Press.
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英文
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甘蓝型油菜氮素营养高效种质的鉴定与评价方法
作者：
邹小云;刘宝林;李俊;宋来强;官春云
作者机构：
[邹小云; 刘宝林; 李俊; 宋来强; 官春云] 江西省农业科学院作物研究所/江西省油料作物生物学重点实验室;[邹小云; 刘宝林; 李俊; 宋来强; 官春云] 湖南农业大学油料作物研究所/国家油料作物改良中心湖南分中心;[邹小云; 刘宝林; 李俊; 宋来强; 官春云] 中国农业科学院油料作物研究所
会议名称：
中国作物学会油料作物专业委员会第八次会员代表大会暨学术年会
会议时间：
2018-11-19
会议地点：
中国山东青岛
关键词：
甘蓝型油菜;氮肥利用率;抽薹期;氮素营养高效种质;选择指标;单株角果数
摘要：
油菜是一种适应性强、发展潜力大的油料作物,在世界油料作物中,面积和总产量都位居第二位。氮肥在油菜生产中起了关键的作用,但氮肥的利用率很低,平均只有30%～35%。因此,必须降低油菜氮肥施用量,提高氮肥利用率。当前增加油菜籽粒产量和提高氮肥利用率的一条有效途径是培育和种植氮营养高效油菜品种。国内外学者已在许多物种上进行了氮高效种质的筛选,为后人提供了丰富可靠的理论参考依据,但是对选择压力、筛选时期及
语种：
中文
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甘蓝型油菜种质氮素营养效率的鉴定及评价指标筛选
作者：
邹小云;刘宝林;李俊;宋来强;官春云
期刊：
中国油料作物学报 ,2018年40(2):247-257 ISSN：1007-9084
作者机构：
江西省农业科学院作物研究所, 江西省油料作物生物学重点实验室, 江西, 南昌, 330200;湖南农业大学油料作物研究所/国家油料作物改良中心湖南分中心, 湖南, 长沙, 410128;中国农业科学院油料作物研究所, 湖北, 武汉, 430062;[邹小云] 江西省农业科学院作物研究所, 江西省油料作物生物学重点实验室, 江西, 南昌, 330200 湖南农业大学油料作物研究所/国家油料作物改良中心湖南分中心, 湖南, 长沙, 410128;[刘宝林; 官春云] 湖南农业大学油料作物研究所/国家油料作物改良中心湖南分中心, 湖南, 长沙, 410128
关键词：
甘蓝型油菜;种质;氮素营养效率;单株籽粒重;单株地上干重;茎基粗
摘要：
为建立甘蓝型油菜种质氮素营养效率的鉴定及评价方法,筛选合理的次级评价指标和氮素营养高效基因型,在田间小区试验条件下设置低氮(45kg/hm~2)、中氮(180kg/hm~2)和高氮(270kg/hm~2) 3个施氮水平,测定了416份不同生态类型甘蓝型油菜种质植株性状及氮素吸收效率(NAE)、氮素利用效率(NUE)和氮收获指数(NHI) 。各性状在不同氮素水平下对氮的敏感性不同,低氮下第一次有效分枝数的变异程度最大,单株籽粒重次之;中氮和高氮处理水平下单株籽粒重的变异程度均表现为最大,低氮胁迫加大了种质间的差异。油菜种质氮素营养效率基因型间差异明显,表现中效类型的种质最多,高效和低效的较少。油菜种质间成熟期NAE的鉴定与评价应选择在低氮处理下,以单株籽粒重、单株地上干重和株高为间接指标进行选择效果明显;油菜种质间成熟期NUE的鉴定与评价应选择在高氮处理下,以单株地上干重、第一次有效分枝数、单株籽粒重和每角粒数为间接指标进行选择效果明显;油菜种质间成熟期NHI的鉴定与评价应选择在低氮处理下,以单株籽粒重、单株地上干重和茎基粗为间接指标进行选择效果明显。
语种：
中文
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Temperature determines the diversity and structure of N2O-reducing microbial assemblages
作者：
Wu, Bo;Liu, Feifei;Weiser, Michael D.;Ning, Daliang;Okie, Jordan G.;...
期刊：
Functional Ecology ,2018年32(7):1867-1878 ISSN：0269-8463
通讯作者：
Shouwen Chen<&wdkj&>Jizhong Zhou<&wdkj&>Zhili He
作者机构：
[Wu, Bo; He, Zhili] Sun Yat Sen Univ, Environm Microbi Res Ctr & Guangdong Prov Key Lab, Guangzhou, Peoples R China.;[Deng, Ye; Wu, Bo; Wu, Liyou; Ning, Daliang; He, Zhili; Zhou, Jizhong; Liu, Feifei; Shen, Lina] Univ Oklahoma, Inst Environm Genom & Dept Microbiol & Plant Biol, Norman, OK USA.;[Wu, Bo; He, Zhili; Chen, Shouwen] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan, Peoples R China.;[Liu, Feifei] Guangdong Inst Microbiol & State Key Lab Appl Mic, Guangdong Prov Key Lab Microbial Culture Collect, Guangzhou, Peoples R China.;[Weiser, Michael D.] Univ Oklahoma, Dept Biol, Norman, OK USA.
通讯机构：
[Shouwen Chen] S;[Jizhong Zhou] I;[Zhili He] E;State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China<&wdkj&>Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan, China<&wdkj&>Environmental Microbiomics Research Center and Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, China<&wdkj&>Institute for Environmental Genomics and Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA<&wdkj&>State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China<&wdkj&>College of Agriculture, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>Institute for Environmental Genomics and Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA<&wdkj&>Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA<&wdkj&>School of Environment, Tsinghua University, Beijing, China
关键词：
biogeography;diversity;latitudinal diversity gradient;N2O-reducing community;nosZ;temperature gradient
摘要：
Micro-organisms harbouring the nosZ gene convert N2O to N2 and play a critical role in reducing global N2O emissions. As higher denitrifier diversity can result in higher denitrification rates, here we aimed to understand the diversity, composition and spatial structure of N2O-reducing microbial assemblages in forest soils across a large latitudinal and temperature gradient. We sequenced nosZ gene amplicons of 126 soil samples from six forests with mean annual soil temperatures (MAST) ranging from 3.7 to 25.3°C and tested predictions of the metabolic theory of ecology (MTE) and metabolic-niche theory (MNT). As predicted, α-diversity of nosZ communities increased with increasing MAST, within-site β-diversity decreased and two (pH and soil moisture) of the three niche widths examined were larger with increasing MAST. We calculated β-nearest taxon distance and Raup–Crick metric to quantify the relative influence of the assembly processes determining nosZ assemblage structure. Environmental selection was the primary process driving assemblage structure in all six forests. Homogenizing dispersal was also important at one site, which could be explained by the site's much lower variability in soil chemistry. We used canonical correspondence analysis and multiple regression on matrices to examine relationships between nosZ communities and environmental factors, and found that temperature and spatial distance were significant predictors of nosZ assemblage structure. Overall our results support both theories (MTE and MNT) tested, showing that higher temperatures are correlated with higher local diversity, wider niche breadths and lower within-site turnover rates. A plain language summary is available for this article. © 2018 The Authors. Functional Ecology © 2018 British Ecological Society
语种：
英文
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The role of soil bacterial community during winter fallow period in the incidence of tobacco bacterial wilt disease
作者：
Xiao, Yunhua;Liu, Xueduan;Meng, Delong;Tao, Jiemeng;Gu, Yabing;...
期刊：
Applied Microbiology and Biotechnology ,2018年102(5):2399-2412 ISSN：0175-7598
通讯作者：
Li, Juan;Yin, Huaqun
作者机构：
[Xiao, Yunhua; Li, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Xiao, Yunhua; Li, Juan] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Meng, Delong; Liu, Xueduan; Yin, Huaqun; Xiao, Yunhua; Tao, Jiemeng; Gu, Yabing] Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China.
通讯机构：
[Li, Juan] H;[Yin, Huaqun] C;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China.
关键词：
Crop morbidity;Biological control agents;Bacterial interactions;Bacterial diversity;Bacterial community structure
摘要：
Bacterial wilt, caused by Ralstonia solanacearum, occurs occasionally during tobacco planting and potentially brings huge economic losses in affected areas. Soil microbes in different management stages play important roles in influencing bacterial wilt incidence. Studies have focused on the impacts of species diversity and composition during cropping periods on disease morbidity; however, the effects of the soil bacterial biomass, species diversity, species succession, and population interactions on morbidity remain unclear during non-cropping periods. In this study, we explored the soil bacterial communities in the non-cropping winter fallow (WF) and cropping late growing (LG) periods under consecutive monoculture systems using 16S ribosomal RNA gene sequencing and qPCR and further analyzed their effects on tobacco bacterial wilt incidence. We found that the bacterial communities in the WF period were significantly different from those in the LG period based on detrended correspondence analysis and dissimilarity tests. Crop morbidity was significantly related to bacterial community structure and to the presence of some genera during WF and LG periods. These genera, such as Arthrobacter, Pseudomonas, Acidobacteria GP6, and Pasteuria, may be potential biological control agents for bacterial wilt. Further analysis indicated that low soil bacterial diversity during the WF period, decrease of bacterial interactions from the WF to LG periods, and low soil biomass during the LG period all have the potential to increase morbidity. In conclusion, an increase of soil bacterial diversity and control of some bacterial abundances in the WF period might be an effective approach in controlling bacterial wilt incidence. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
语种：
英文
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Integrated network analysis reveals the importance of microbial interactions for maize growth
作者：
Tao, Jiemeng;Meng, Delong;Qin, Chong;Liu, Xueduan;Liang, Yili;...
期刊：
Applied Microbiology and Biotechnology ,2018年102(8):3805-3818 ISSN：0175-7598
通讯作者：
Yin, Huaqun;Li, Juan
作者机构：
[Qin, Chong; Meng, Delong; Liu, Xueduan; Yin, Huaqun; Liang, Yili; Tao, Jiemeng; Gu, Yabing; Liu, Zhenghua] Cent S Univ, Sch Minerals Proc & Bioengn, Changsha, Hunan, Peoples R China.;[Qin, Chong; Meng, Delong; Liu, Xueduan; Yin, Huaqun; Liang, Yili; Gu, Yabing; Liu, Zhenghua] Cent S Univ, Minist Educ, Key Lab Biomet, Changsha, Hunan, Peoples R China.;[Xiao, Yunhua; Li, Juan; Tao, Jiemeng] Hunan Agr Univ, Coll Agron, Changsha, Hunan, Peoples R China.
通讯机构：
[Yin, Huaqun] C;[Li, Juan] H;Cent S Univ, Sch Minerals Proc & Bioengn, Changsha, Hunan, Peoples R China.;Cent S Univ, Minist Educ, Key Lab Biomet, Changsha, Hunan, Peoples R China.;Hunan Agr Univ, Coll Agron, Changsha, Hunan, Peoples R China.
关键词：
Green manures;Microbial networks;Microbial interactions;Keystone species;Maize yield
摘要：
Microbes play a critical role in soil global biogeochemical circulation and microbe–microbe interactions have also evoked enormous interests in recent years. Utilization of green manures can stimulate microbial activity and affect microbial composition and diversity. However, few studies focus on the microbial interactions or detect the key functional members in communities. With the advances of metagenomic technologies, network analysis has been used as a powerful tool to detect robust interactions between microbial members. Here, random matrix theory-based network analysis was used to investigate the microbial networks in response to four different green manure fertilization regimes (Vicia villosa, common vetch, milk vetch, and radish) over two growth cycles from October 2012 to September 2014. The results showed that the topological properties of microbial networks were dramatically altered by green manure fertilization. Microbial network under milk vetch amendment showed substantially more intense complexity and interactions than other fertilization systems, indicating that milk vetch provided a favorable condition for microbial interactions and niche sharing. The shift of microbial interactions could be attributed to the changes in some major soil traits and the interactions might be correlated to plant growth and production. With the stimuli of green manures, positive interactions predominated the network eventually and the network complexity was in consistency with maize productivity, which suggested that the complex soil microbial networks might benefit to plants rather than simple ones, because complex networks would hold strong the ability to cope with environment changes or suppress soil-borne pathogen infection on plants. In addition, network analyses discerned some putative keystone taxa and seven of them had directly positive interactions with maize yield, which suggested their important roles in maintaining environmental functions and in improving plant growth. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
语种：
英文
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Response of soil microbial communities and microbial interactions to long-term heavy metal contamination
作者：
Li, Xiaoqi;Meng, Delong;Li, Juan;Yin, Huaqun;Liu, Hongwei;...
期刊：
Environmental Pollution ,2017年231(Dec. Pt.1):908-917 ISSN：0269-7491
通讯作者：
Yan, Mingli
作者机构：
[Li, Xiaoqi; Liu, Hongwei; Meng, Delong; Yin, Huaqun; Liu, Xueduan; Xiao, Yunhua; Liu, Zhenghua] Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China.;[Li, Juan] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Li, Xiaoqi; Liu, Hongwei; Meng, Delong; Yin, Huaqun; Liu, Xueduan; Xiao, Yunhua; Liu, Zhenghua] Minist Educ, Key Lab Biomet, Changsha 410083, Hunan, Peoples R China.;[Cheng, Cheng; Yan, Mingli] Hunan Univ Sci & Technol, Sch Life Sci, Xiangtan 411201, Hunan, Peoples R China.;[Meng, Delong] Univ Coll Dublin, Sch Biol & Environm Sci, Dublin 4, Ireland.
通讯机构：
[Yan, Mingli] H;Hunan Univ Sci & Technol, Sch Life Sci, Xiangtan 411201, Hunan, Peoples R China.
关键词：
Microbial community;Molecular ecological networks;Heavy metal contamination;Microbial interactions
摘要：
Due to the persistence of metals in the ecosystem and their threat to all living organisms, effects of heavy metal on soil microbial communities were widely studied. However, little was known about the interactions among microorganisms in heavy metal-contaminated soils. In the present study, microbial communities in Non (CON), moderately (CL) and severely (CH) contaminated soils were investigated through high-throughput Illumina sequencing of 16s rRNA gene amplicons, and networks were constructed to show the interactions among microbes. Results showed that the microbial community composition was significantly, while the microbial diversity was not significantly affected by heavy metal contamination. Bacteria showed various response to heavy metals. Bacteria that positively correlated with Cd, e.g. Acidobacteria_Gp and Proteobacteria_thiobacillus, had more links between nodes and more positive interactions among microbes in CL- and CH-networks, while bacteria that negatively correlated with Cd, e.g. Longilinea, Gp2 and Gp4 had fewer network links and more negative interactions in CL and CH-networks. Unlike bacteria, members of the archaeal domain, i.e. phyla Crenarchaeota and Euryarchaeota, class Thermoprotei and order Thermoplasmatales showed only positive correlation with Cd and had more network interactions in CH-networks. The present study indicated that (i) the microbial community composition, as well as network interactions was shift to strengthen adaptability of microorganisms to heavy metal contamination, (ii) archaea were resistant to heavy metal contamination and may contribute to the adaption to heavy metals. It was proposed that the contribution might be achieved either by improving environment conditions or by cooperative interactions. © 2017 Elsevier Ltd
语种：
英文
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An integrated insight into the relationship between soil microbial community and tobacco bacterial wilt disease
作者：
Yang, Hongwu;Li, Juan;Xiao, Yunhua;Gu, Yabing;Liu, Hongwei;...
期刊：
Frontiers in Microbiology ,2017年8(NOV):2179 ISSN：1664-302X
通讯作者：
Meng, Delong;Yin, Huaqun
作者机构：
[Xiao, Yunhua; Yang, Hongwu; Li, Juan] Hunan Agr Univ, Coll Agron, Changsha, Hunan, Peoples R China.;[Liu, Hongwei; Meng, Delong; Liu, Xueduan; Yin, Huaqun; Xiao, Yunhua; Liang, Yili; Meng, DL; Hu, Jin; Gu, Yabing] Cent S Univ, Sch Minerals Proc & Bioengn, Changsha, Hunan, Peoples R China.;[Liu, Hongwei; Meng, Delong; Liu, Xueduan; Yin, Huaqun; Xiao, Yunhua; Liang, Yili; Meng, DL; Hu, Jin; Gu, Yabing] Minist Educ, Key Lab Biomet, Changsha, Hunan, Peoples R China.
通讯机构：
[Meng, DL; Yin, HQ] C;[Meng, DL; Yin, HQ] M;Cent S Univ, Sch Minerals Proc & Bioengn, Changsha, Hunan, Peoples R China.;Minist Educ, Key Lab Biomet, Changsha, Hunan, Peoples R China.
关键词：
microbial community composition;molecular ecology networks;plant health;soil microbial community;tobacco wilt disease
摘要：
The soil microbial communities play an important role in plant health, however, the relationship between the below-ground microbiome and above-ground plant health remains unclear. To reveal such a relationship, we analyzed soil microbial communities through sequencing of 16S rRNA gene amplicons from 15 different tobacco fields with different levels of wilt disease in the central south part of China. We found that plant health was related to the soil microbial diversity as plants may benefit from the diverse microbial communities. Also, those 15 fields were grouped into 'healthy' and 'infected' samples based upon soil microbial community composition analyses such as unweighted paired-group method with arithmetic means (UPGMA) and principle component analysis, and furthermore, molecular ecological network analysis indicated that some potential plant-beneficial microbial groups, e.g., Bacillus and Actinobacteria could act as network key taxa, thus reducing the chance of plant soil-borne pathogen invasion. In addition, we propose that a more complex soil ecology network may help suppress tobacco wilt, which was also consistent with highly diversity and composition with plant-beneficial microbial groups. This study provides new insights into our understanding the relationship between the soil microbiome and plant health. © 2017 Yang, Li, Xiao, Gu, Liu, Liang, Liu, Hu, Meng and Yin.
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英文
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The high-quality genome of Brassica napus cultivar ‘ZS11’reveals the introgression history in semi-winter morphotype
作者：
Sun, Fengming;Fan, Guangyi;Hu, Qiong;Zhou, Yongming;Guan, Mei;...
期刊：
PLANT JOURNAL ,2017年92(3):452-468 ISSN：0960-7412
通讯作者：
Chalhoub, Boulos(chalhoub@evry.inra.fr)
作者机构：
[Wang, Xinfa; Wang, Hanzhong; Dong, Caihua; Tong, Chaobo; Liu, Shengyi; Hu, Qiong; Sun, Fengming; Mei, Desheng; Hu, Zhiyong; Yu, Jingyin; Huang, Shunmou; Li, Jun; Shi, Jiaqin; Hua, Wei] Chinese Acad Agr Sci, Oil Crops Res Inst, Minist Agr PRC, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Hubei, Peoples R China.;[Shi, Chengcheng; Xu, Xun; Liang, Xinming; Chen, Wenbin; Huang, Guodong; Wang, Jiahao; Wang, Xi; Zhang, He; Fan, Guangyi; Li, Liangwei; Zeng, Peng; Zhang, Yaolei; Liu, Weiqing; Liu, Xin] Beijing Genome Inst Shenzhen, Shenzhen 518083, Peoples R China.;[Liu, Kede; Meng, Jinling; Long, Yan; Zhou, Yongming] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China.;[Guan, Chunyun; Guan, Mei] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Chalhoub, Boulos] OEPG, UMR1165, URGV, INRA, 2 Rue Gaston Cremieux, F-91057 Evry, France.
通讯机构：
[Boulos Chalhoub] I;[Wei Hua; Hanzhong Wang] T;Institut National de Recherche Agronomique (INRA), Unité de Recherche en Génomique Végétale (URGV), UMR1165, Organization and Evolution of Plant Genomes (OEPG), 2 rue Gaston Crémieux, 91057 Evry, France<&wdkj&>The Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture of the PRC, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062 China
关键词：
Brassica napus;allotetraploid;genome;homoeolog;introgression;next-generation sequencing technologies;polyploid;subgenome;ZS11
摘要：
Allotetraploid oilseed rape (Brassica napus L.) is an agriculturally important crop. Cultivation and breeding of B. napus by humans has resulted in numerous genetically diverse morphotypes with optimized agronomic traits and ecophysiological adaptation. To further understand the genetic basis of diversification and adaptation, we report a draft genome of an Asian semi-winter oilseed rape cultivar 'ZS11' and its comprehensive genomic comparison with the genomes of the winter-type cultivar 'Darmor-bzh' as well as two progenitors. The integrated BAC-to-BAC and whole-genome shotgun sequencing strategies were effective in the assembly of repetitive regions (especially young long terminal repeats) and resulted in a high-quality genome assembly of B. napus 'ZS11'. Within a short evolutionary period (similar to 6700years ago), semi-winter-type 'ZS11' and the winter-type 'Darmor-bzh' maintained highly genomic collinearity. Even so, certain genetic differences were also detected in two morphotypes. Relative to Darmor-bzh', both two subgenomes of 'ZS11' are closely related to its progenitors, and the 'ZS11' genome harbored several specific segmental homoeologous exchanges (HEs). Furthermore, the semi-winter-type 'ZS11' underwent potential genomic introgressions with B. rapa (A(r)). Some of these genetic differences were associated with key agronomic traits. A key gene of A03.FLC3 regulating vernalization-responsive flowering time in 'ZS11' was first experienced HE, and then underwent genomic introgression event with A(r), which potentially has led to genetic differences in controlling vernalization in the semi-winter types. Our observations improved our understanding of the genetic diversity of different B. napus morphotypes and the cultivation history of semi-winter oilseed rape in Asia. Significance Statement We generated a high-quality reference genome sequence of semi-winter Brassica napus. Our BAC-to-BAC strategy was effective in the assembly of repetitive regions. The main phenotypic difference between semi-winter and winter B. napus morphotypes is the vernalization requirement. Here, we found that a key gene controlling the vernalization-responsive flowering time was altered in the semi-winter type ZS11' through both homoeologous exchanges and genomic introgression with B. rapa.
语种：
英文
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Quantitative proteomic analysis of upland cotton stem terminal buds reveals phytohormone-related pathways associated with dwarfism
作者：
Tu, X.;Li, J.;Wang, Q.* ;Liu, A.*
期刊：
BIOLOGIA PLANTARUM ,2017年61(1):106-114 ISSN：0006-3134
通讯作者：
Liu, A.;Wang, Q.
作者机构：
[Tu, X.] Hunan Agr Univ, Coll Orient Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Liu, A.; Tu, X.; Li, J.] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Wang, Q.] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, A.; Wang, Q.] H;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词：
auxins;cytokinins;gibberellins;Gossypium hirsutum;iTRAQ;near-isogenic line
摘要：
Identifying important regulative elements and pathways related to dwarfism in cotton is a major challenge in cotton breeding. Isobaric tags for relative and absolute quantitation-based proteomics of stem terminal buds from upland cotton (Gossypium hirsutum L.) dwarf line LA-1 and high near-isogenic line LH-1 was performed. Moreover, transcriptional expression of differentially-expressed proteins (DEPs) belonging to phytohormone signal transduction, hormone biosynthesis, and ubiquitin system were analysed using quantitative real-time polymerase chain reaction. A total of 4 849 proteins were identified from LA-1 and LH-1, 697 of which showed differential accumulations. Most of the DEPs have catalytic, binding, and transporter activity and are involved in metabolism- and protein processing-related pathways. In particular, seven DEPs, including two gibberellin (GA) receptors, three cytokinin (CK) receptors, CKoxidase, and CK-N-glucosyltransferase were up-regulated in LA-1, and GA20-oxidase was down-regulated in LH-1. Our results suggest that the DELLA-independent GAsignalling pathway is the primary cause of dwarfism in LA-1 and indicate that CKresponse element 1-2, GA-insensitive dwarf, CKoxidase, and GA3-β-dioxygenase are potential indicators of dwarf cotton. The profiling of DEPs may offer a valuable resource for cotton breeding. © 2017, Springer Science+Business Media Dordrecht.
语种：
英文
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Analysis of intergeneric sexual hybridization between transgenic Brassica oleracea and Sinapis alba
作者：
Li, Jun;Zhang, Chunlei;Guan, Chunyun;Luo, Lixia;Ren, Li;...
期刊：
Euphytica ,2017年213(12):1-10 ISSN：0014-2336
通讯作者：
Lu, Guangyuan;Fang, Xiaoping
作者机构：
[Li, Jun; Lu, Guangyuan; Wei, Wenhui] Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr, Oil Crops Res Inst, Wuhan, Hubei, Peoples R China.;[Li, Jun; Fang, Xiaoping; Ren, Li; Zhang, Chunlei; Luo, Lixia] Chinese Acad Agr Sci, Key Lab Crop Cultivat & Physiol, Minist Agr, Oil Crops Res Inst, Wuhan, Hubei, Peoples R China.;[Guan, Chunyun] Hunan Agr Univ, Coll Agr, Changsha, Hunan, Peoples R China.;[Lu, Guangyuan] Hubei Engn Univ, New Rural Dev Inst, Xiaogan, Peoples R China.
通讯机构：
[Lu, Guangyuan; Fang, Xiaoping] C;Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr, Oil Crops Res Inst, Wuhan, Hubei, Peoples R China.;Chinese Acad Agr Sci, Key Lab Crop Cultivat & Physiol, Minist Agr, Oil Crops Res Inst, Wuhan, Hubei, Peoples R China.
关键词：
Brassica oleracea;Sinapis alba;Intergeneric hybridization;Bar gene;Disease resistance;Alternaria brassicae
摘要：
Sinapis alba possesses a number of desirable traits that can be employed to broaden genetic variability in Brassica oleracea or B. napus. Using transgenic B. oleracea (CC, 2n = 18) as paternal plants and non-transgenic S. alba (SS, 2n = 24) as maternal plants, 11 hybrid plants were successfully obtained by using a combination of ovary culture and embryo rescue, while 2 seedlings of the reciprocal cross were generated but lost during the propagation. The hybridity of these plants was confirmed by flower color and other agronomic characteristics, chromosome counting, and pollen viability. Most of the intergeneric F1 plants showed only one set each of S and C chromosome (SC, 2n = 21) and were male sterile, while a few plants were semi-fertile and had one set of S chromosomes but two sets of C chromosomes (CCS, 2n = 30). PCR, genomic Southern blotting and qRT-PCR for bar gene revealed that among the F1 hybrid plants only 9.1% was bar positive and could be forwarded to F2 and F3 generation. The majority of F3 plants obtained sufficient resistance to Alternaria brassicae. Thus, the generation of disease (A. brassicae) and herbicide (Bastar) resistant intergeneric hybrids was of importance for breeding program. The existence of bar gene in these intermediate materials will facilitate the identification of hybridity and transfer of S.alba traits into target genetic background. © 2017, The Author(s).
语种：
英文
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HuMiChip2 for strain level identification and functional profiling of human microbiomes
作者：
Tu, Qichao;Li, Jiabao;Shi, Zhou;Chen, Yanfei;Lin, Lu;...
期刊：
Applied Microbiology and Biotechnology ,2017年101(1):423-435 ISSN：0175-7598
通讯作者：
Zhou, Jizhong;He, Zhili
作者机构：
[Lin, Lu; Tu, Qichao] Zhejiang Univ, Dept Marine Sci, Ocean Coll, Hangzhou, Zhejiang, Peoples R China.;[Yan, Jianbo; Tu, Qichao; Wang, Hongling] Zhoushan Municipal Ctr Dis Control & Prevent, Zhoushan, Zhejiang, Peoples R China.;[Shi, Zhou; Tu, Qichao; He, Zhili; Zhou, Jizhong; Zhou, JZ] Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA.;[Li, Jiabao; Li, Xiangzhen] Chinese Acad Sci, Chengdu Inst Biol, Chengdu, Sichuan, Peoples R China.;[Chen, Yanfei; Li, Lanjuan] Zhejiang Univ, Affiliated Hosp 1, Hangzhou, Zhejiang, Peoples R China.
通讯机构：
[Zhou, JZ; He, ZL] U;Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA.
关键词：
HuMiChip2;Microbial ecological microarray;Strain-level identification;Functional profiling;Human microbiome
摘要：
With the massive data generated by the Human Microbiome Project, how to transform such data into useful information and knowledge remains challenging. Here, with currently available sequencing information (reference genomes and metagenomes), we have developed a comprehensive microarray, HuMiChip2, for strain-level identification and functional characterization of human microbiomes. HuMiChip2 was composed of 29,467 strain-specific probes targeting 2063 microbial strains/species and 133,924 sequence- and group-specific probes targeting 157 key functional gene families involved in various metabolic pathways and host-microbiome interaction processes. Computational evaluation of strain-specific probes suggested that they were not only specific to mock communities of sequenced microorganisms and metagenomes from different human body sites but also to non-sequenced microbial strains. Experimental evaluation of strain-specific probes using single strains/species and mock communities suggested a high specificity of these probes with their corresponding targets. Application of HuMiChip2 to human gut microbiome samples showed the patient microbiomes of alcoholic liver cirrhosis significantly (p < 0.05) shifted their functional structure from the healthy individuals, and the relative abundance of 21 gene families significantly (p < 0.1) differed between the liver cirrhosis patients and healthy individuals. At the strain level, five Bacteroides strains were significantly (p < 0.1) and more frequently detected in liver cirrhosis patients. These results suggest that the developed HuMiChip2 is a useful microbial ecological microarray for both strain-level identification and functional profiling of human microbiomes.
语种：
英文
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National-scale paddy-upland rotation in Northern China promotes sustainable development of cultivated land
作者：
Zheng, Huabin;Huang, Huang* ;Zhang, Canming* ;Li, Jingyi
期刊：
Agricultural Water Management ,2016年170:20-25 ISSN：0378-3774
通讯作者：
Huang, Huang;Zhang, Canming
作者机构：
[Zheng, Huabin; Li, Jingyi; Huang, Huang] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Zheng, Huabin; Li, Jingyi; Huang, Huang] Collaborat Innovat Ctr Grain & Oil Crop Southern, Changsha 410128, Hunan, Peoples R China.;[Zhang, Canming] Asia Europe Meeting ASEM Water Resources Res & De, Changsha 410013, Hunan, Peoples R China.
通讯机构：
[Huang, Huang] H;[Zhang, Canming] A;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;Asia Europe Meeting ASEM Water Resources Res & De, Changsha 410013, Hunan, Peoples R China.
关键词：
Paddy-upland rotation;Water resource;Cultivated land;China
摘要：
As China requires more cultivated land to feed its increasing population, the sustainable development of cultivated land in its intensive agricultural production system has become a major issue for government and society. We firstly reviewed the progress of paddy-upland rotation (PUR) and conservation tillage, found that PUR was improve significantly soil properties, including redox potential of soil, the mineralization of soil organic matter, and the abundance of beneficial microorganisms, suggested that conservation tillage was an effective measurement for remedying the deficiency under the PUR. Meanwhile, we also systemically reviewed the statues of water resource infrastructure and policy level. Consequently, with current agriculture technology and the increasing capacity of adjusted artificial water resources, our results highlight the development of national-scale PUR as an effective adaptation strategy to protect and improve soil fertility and ensure agricultural security under climate change. In other words, from national strategy scale, national-scale PUR was a good choice in the major grain-producing areas of northern China in the coming 50-100 years. Lastly, further research in the few aspects was needed to better provide theoretical and technical support for promoting effectively the PUR. © 2016 Elsevier B.V.
语种：
英文
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Genome-wide association study provides insight into the genetic control of plant height in rapeseed (Brassica napus L.)
作者：
Sun, Chengming;Wang, Benqi;Yan, Lei;Hu, Kaining;Liu, Sheng;...
期刊：
FRONTIERS IN PLANT SCIENCE ,2016年7(JULY2016):1102 ISSN：1664-462X
通讯作者：
Yi, Bin
作者机构：
[Wang, Benqi; Tu, Jinxing; Yan, Lei; Liu, Sheng; Sun, Chengming; Hu, Kaining; Shen, Jinxiong; Ma, Chaozhi; Wen, Jing; Yi, Bin; Zhou, Yongming; Fu, Tingdong] Huazhong Agr Univ, Natl Subctr Rapeseed Improvement Wuhan, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China.;[Guan, Chunyun; Zhang, Zhenqian] Hunan Agr Univ, Coll Agron, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha, Hunan, Peoples R China.;[Li, Jiana] Southwest Univ, Coll Agron & Biotechnol, Chongqing Rapeseed Engn Technol Res Ctr, Chongqing, Peoples R China.;[Zhang, Jiefu; Chen, Song] Jiangsu Acad Agr Sci, Key Lab Cotton & Rapeseed, Nanjing, Jiangsu, Peoples R China.
通讯机构：
[Yi, Bin] H;Huazhong Agr Univ, Natl Subctr Rapeseed Improvement Wuhan, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China.
关键词：
Brassica napus;association mapping;haplotype block;plant height;selective sweep
摘要：
Plant height is a key morphological trait of rapeseed. In this study, we measured plant height of a rapeseed population across six environments. This population contains 476 inbred lines representing the major Chinese rapeseed genepool and 44 lines from other countries. The 60K Brassica Infinium® SNP array was utilized to genotype the association panel. A genome-wide association study (GWAS) was performed via three methods, including a robust, novel, nonparametric Anderson-Darling (A-D) test. Consequently, 68 loci were identified as significantly associated with plant height (P < 5.22 × 10−5), and more than 70% of the loci (48) overlapped the confidence intervals of reported QTLs from nine mapping populations. Moreover, 24 GWAS loci were detected with selective sweep signals, which reflected the signatures of historical semi-dwarf breeding. In the linkage disequilibrium (LD) decay range up—and downstream of 65 loci (r2 > 0.1), we found plausible candidates orthologous to the documented Arabidopsis genes involved in height regulation. One significant association found by GWAS colocalized with the established height locus BnRGA in rapeseed. Our results provide insights into the genetic basis of plant height in rapeseed and may facilitate marker-based breeding. © 2016 Sun, Wang, Yan, Hu, Liu, Zhou, Guan, Zhang, Li, Zhang, Chen, Wen, Ma, Tu, Shen, Fu and Yi.
语种：
英文
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Constructing a high-density linkage map for Gossypium hirsutum×Gossypium barbadense and identifying QTLs for lint percentage
作者：
Shi, Yuzhen;Li, Wentan;Li, Aiguo;Ge, Ruihua;Zhang, Baocai;...
期刊：
植物学报：英文版 ,2015年57(5):450-467 ISSN：1672-9072
通讯作者：
Yuan, Youlu
作者机构：
[Li, Wentan; Yuan, Youlu; Wang, Wei; Ge, Ruihua; Chen, Tingting; Wang, Kunbo; Shi, Yuzhen; Liu, Guangping; Li, Junwen; Wang, Tao; Li, Junzhi; Liu, Aiying; Gong, Wankui; Li, Aiguo; Gong, Juwu; Zhang, Baocai; Shang, Haihong; Yang, Zemao] Chinese Acad Agr Sci, Key Lab Biol & Genet Breeding Cotton, State Key Lab Cotton Biol, Minist Agr,Inst Cotton Res, Anyang 455000, Peoples R China.;[Li, Wentan; Tang, Feiyu] Jiangxi Agr Univ, Coll Agr, Nanchang 330045, Peoples R China.;[Li, Aiguo] Acad Agr Sci Luohe City Henan Prov, Luohe 462000, Peoples R China.;[Liu, Zhi; Ge, Ruihua; Liu, Guangping; Jiang, Jianxiong; Li, Junzhi; Zhu, Weiping; Yang, Zemao] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Yu, Xiaonan] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Yuan, Youlu] C;Chinese Acad Agr Sci, Key Lab Biol & Genet Breeding Cotton, State Key Lab Cotton Biol, Minist Agr,Inst Cotton Res, Anyang 455000, Peoples R China.
关键词：
Cotton (Gossypium spp.);genetic linkage map;Gossypium barbadense;lint percentage;simple sequence repeat
摘要：
To introgress the good fiber quality and yield from Gossypium barbadense into a commercial Upland cotton variety, a high-density simple sequence repeat (SSR) genetic linkage map was developed from a BC1F1 population of Gossypium hirsutum×Gossypium barbadense. The map comprised 2,292 loci and covered 5115.16 centiMorgan (cM) of the cotton AD genome, with an average marker interval of 2.23 cM. Of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on the D genome sequence genetic map. Compared with five published high-density SSR genetic maps, 53.14% of marker loci were newly discovered in this map. Twenty-six quantitative trait loci (QTLs) for lint percentage (LP) were identified on nine chromosomes. Nine stable or common QTLs could be used for marker-assisted selection. Fifty percent of the QTLs were from G. barbadense and increased LP by 1.07%-2.41%. These results indicated that the map could be used for screening chromosome substitution segments from G. barbadense in the Upland cotton background, identifying QTLs or genes from G. barbadense, and further developing the gene pyramiding effect for improving fiber yield and quality. © 2014 Institute of Botany, Chinese Academy of Sciences.
语种：
英文
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Genome-wide association study reveals the genetic architecture of flowering time in rapeseed (Brassica napus L.)
作者：
Xu, Liping;Hu, Kaining;Zhang, Zhenqian;Guan, Chunyun;Chen, Song;...
期刊：
DNA RESEARCH ,2015年23(1):43-52 ISSN：1340-2838
通讯作者：
Yi, Bin
作者机构：
[Tu, Jinxing; Hu, Kaining; Shen, Jinxiong; Ma, Chaozhi; Xu, Liping; Wen, Jing; Yi, Bin; Fu, Tingdong] Huazhong Agr Univ, Natl Ctr Rapeseed Improvement, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China.;[Guan, Chunyun; Zhang, Zhenqian] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Chen, Song] Jiangsu Acad Agr Sci, Nanjing 210014, Jiangsu, Peoples R China.;[Hua, Wei] Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Peoples R China.;[Li, Jiana] Southwest Univ, Coll Agron & Biotechnol, Chongqing Engn Res Ctr Rapeseed, Chongqing 400716, Peoples R China.
通讯机构：
[Yi, Bin] H;Huazhong Agr Univ, Natl Ctr Rapeseed Improvement, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China.
关键词：
Brassica napus;flowering time;SNP;association mapping;linkage disequilibrium
摘要：
Flowering time adaptation is a major breeding goal in the allopolyploid species Brassica napus. To investigate the genetic architecture of flowering time, a genome-wide association study (GWAS) of flowering time was conducted with a diversity panel comprising 523 B. napus cultivars and inbred lines grown in eight different environments. Genotyping was performed with a Brassica 60K Illumina Infinium SNP array. A total of 41 single-nucleotide polymorphisms (SNPs) distributed on 14 chromosomes were found to be associated with flowering time, and 12 SNPs located in the confidence intervals of quantitative trait loci (QTL) identified in previous researches based on linkage analyses. Twenty-five candidate genes were orthologous to Arabidopsis thaliana flowering genes. To further our understanding of the genetic factors influencing flowering time in different environments, GWAS was performed on two derived traits, environment sensitivity and temperature sensitivity. The most significant SNPs were found near Bn-scaff_16362_1-p380982, just 13 kb away from BnaC09g41990D, which is orthologous to A. thaliana CONSTANS (CO), an important gene in the photoperiod flowering pathway. These results provide new insights into the genetic control of flowering time in B. napus and indicate that GWAS is an effective method by which to reveal natural variations of complex traits in B. napus.
语种：
英文
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The brassica oleracea genome reveals the asymmetrical evolution of polyploid genomes
作者：
Liu, Shengyi* ;Liu, Yumei;Yang, Xinhua;Tong, Chaobo;Edwards, David;...
期刊：
Nature Communications ,2014年5(1):3930 ISSN：2041-1723
通讯作者：
Liu, Shengyi
作者机构：
[Huang, Junyan; Hu, Qiong; Hu, Zhiyong; Yu, Jingyin; Liu, Jing; Huang, Shunmou; Wang, Xinfa; Dong, Caihua; Tong, Chaobo; Liu, Shengyi; Zhao, Meixia; Mei, Desheng; Huang, Yi; Shi, Jiaqin; Hua, Wei] Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr PRC, Wuhan 430062, Peoples R China.;[Wu, Jian; Wang, Xiaowu; Fang, Zhiyuan; Liu, Yumei; Cheng, Feng; Wang, Wanxin; Zhuang, Mu; Zhang, Yangyong; Yang, Limei] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Biol & Genet Improvement Hort Crops, Minist Agr, Beijing 10081, Peoples R China.;[Wu, Zhangyan; Yang, Xinhua; Lu, Changxin; Yang, Linfeng; Huang, Yin; Wang, Junyi; Xu, Xun; Liu, Wenbin; Pan, Shengkai; Liang, Xinming; Yue, Zhen; Zhou, Qing; Wang, Jun; Min, Jiumeng; Li, Wanshun; Wang, Zhuo] Beijing Genome Inst Shenzhen, Shenzhen 518083, Peoples R China.;[Edwards, David; Ruperao, Pradeep] Univ Queensland, Australian Ctr Plant Funct Genom, Sch Agr & Food Sci, Brisbane, Qld 4072, Australia.;[Parkin, Isobel A. P.] Agr & Agri Food Canada, Saskatoon, SK S7N OX2, Canada.
通讯机构：
[Liu, Shengyi] C;Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr PRC, Wuhan 430062, Peoples R China.
摘要：
Polyploidization has provided much genetic variation for plant adaptive evolution, but the mechanisms by which the molecular evolution of polyploid genomes establishes genetic architecture underlying species differentiation are unclear. Brassica is an ideal model to increase knowledge of polyploid evolution. Here we describe a draft genome sequence of Brassica oleracea, comparing it with that of its sister species B. rapa to reveal numerous chromosome rearrangements and asymmetrical gene loss in duplicated genomic blocks, asymmetrical amplification of transposable elements, differential gene co-retention for specific pathways and variation in gene expression, including alternative splicing, among a large number of paralogous and orthologous genes. Genes related to the production of anticancer phytochemicals and morphological variations illustrate consequences of genome duplication and gene divergence, imparting biochemical and morphological variation to B. oleracea. This study provides insights into Brassica genome evolution and will underpin research into the many important crops in this genus.
语种：
英文
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