摘要:
Fuzhuan brick tea (FBT), one of the unique dark teas, has various health-promoting functions. In the present study, one polysaccharide fraction, namely FBTPS-2-1, was extracted and purified from FBT, and its structure and potential immunostimulatory activity were investigated. The results showed that FBTPS-2-1,one of typical heteropolysaccharides, was mainly composed of Gal, Ara, and Glc with little molar content of Man, Rha, GalA, and GlcA in molar ratio of 46.59:22.13:13.57:8.20:6.02:2.12:1.38 and molecular weight of 748 kDa. The backbone of FBTPS-2-1 contained -> 4)-beta-D-Galp-(1 -> 4)-beta-D-Galp-(1 ->, -> 4)-beta-D-Galp-(1 -> 4)-alpha-D-Glcp-(1 ->, -> 4)-alpha-D-Glcp-(1 -> 4)-alpha-D-Glcp- (1 ->, -> 4)-alpha-D-Glcp-(1 -> 4)-beta-D-Galp-(1 ->, -> 3)-beta-D-Galp-(1 -> 4)-beta-D-Galp-(1 ->, -> 3,6)-beta-D-Galp- (1 -> 3)-beta-D-Galp- (1 -> and -> 3,6)-beta-D-Galp-(1 -> 3,6)-beta-D-Galp-(1 ->. The linkages of branches in FBTPS-2-1 were mainly composed of alpha-L-Araf-(1 -> 3,6)-beta-D-Galp-(1 ->, -> 5)-alpha-L-Araf-(1 -> 3,6)-beta-D-Galp-(1 ->, -> 6)-beta-D-Galp41 -> 3,6)-beta-D-Galp-(1 ->, alpha-L-Araf-(1 -> 3,S)-alpha-L-Araf-(1 ->, -> 3,5)-alpha-L-Araf- (1 -> 5)-alpha-L-Araf-(1 ->, alpha-D-Galp-(1->3,5)-alpha-L-Araf-(1 -> and -> 5)-alpha-L-Araf-(1 -> 6)-beta-D-Galp-(1 ->. Furthermore, FBTPS-2-1 could increase the phagocytosis of macrophages and promote the secretion of NO and a variety of inflammatory cytokines, including TNF-alpha, IL-1 beta, and IL-6, indicating noticeable immune enhancement activity. Thus, FBTPS-2-1 could serve as a potentially functional food to improve human health by modulating the host immunoreaction.
摘要:
Gelsenicine is one of the most toxic compounds in the genus Gelsemium, but the mechanism of toxicity is not clear. In this paper, tandem mass tag quantitative phosphoproteomics was used to study the changes in protein phosphorylation in different brain regions at different time points after gelsenicine poisoning in mice. The correlation between neurotransmitter receptors and the toxicity of gelsenicine was analyzed by molecular docking and rescue experiments. Parallel reaction monitoring (PRM) was used to verify the related proteins. A total of 17877 unique phosphosites were quantified and mapped to 4170 brain proteins to understand the signaling pathways. Phosphoproteomics revealed gelsenicine poisoning mainly affected protein phosphorylation levels in the hippocampus, and through bioinformatics analysis, it was found gelsenicine poisoning significantly affected neurotransmitter synaptic pathway. The molecular docking results showed that gelsenicine could bind to the N-methyl-D-aspartic acid receptor (NMDAR). In addition, we found that NMDA was effective in improving the survival rate of the animals tested, and this effect was associated with reduced protein phosphorylation by PRM validation. The results revealed that gelsenicine affects neurotransmitter release and receptor function. This is the first demonstration that NMDA receptor-mediated excitotoxicity is a key signaling pathway in the toxicity of gelsenicine.
作者机构:
[Yang, Lulu; Xiao, Xu] Cent South Univ, Business Sch, Changsha 410012, Peoples R China.;[Yang, Lulu] Hunan Agr Univ, Business Sch, Changsha 410128, Peoples R China.;[Gu, Ke] Changsha Univ Sci & Technol, Sch Comp & Commun Engn, Changsha 410114, Peoples R China.
关键词:
agricultural-waste recycling;Material Flow Cost Accounting;family farm;sustainable development
摘要:
In the process of production and operation of family farms, a large amount of agricultural waste, such as livestock and poultry manure, has not been effectively treated in time, causing serious pollution to the environment. Moreover, livestock and poultry manure are the kind of resources that can be recycled to fertilize crops, which can benefit family farms both economically and environmentally. Adoption of manure biogas digesters by family farms can improve sustainability by not only decreasing input use and resource losses, but also reducing environmental pollution. Additionally, Material Flow Cost Accounting (MFCA) is considered to be the most representative environmental management accounting tool. MFCA can be expanded to account for and calculate environmental damages, so as to better reflect the economic and environmental sustainability of agricultural systems. According to the basic principles of material flow cost accounting and characteristics of family farms, we propose an agricultural-waste-recycling model for Chinese family farms that is based on the extended MFCA in this paper. We first investigate Chinese family farms in Hunan Province, and then optimize an agricultural-waste-recycling model by extended MFCA. Finally, based on our proposed model, we make a two-dimensional analysis on the internal resource cost and external environment damages for agricultural-waste recycling. Our analysis shows that visualization of monetization of resource losses can optimize manure recycling through better decision-making, which can increase the sustainability of family farms.
通讯机构:
[Shenghua Ding] H;Hunan Provincial Key Laboratory for Fruits and Vegetables Storage Processing and Quality Safety, Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha, China<&wdkj&>Longping Branch Graduate School, Hunan University, Changsha, China<&wdkj&>Hunan Province International Joint Lab on Fruits & Vegetables Processing, Quality and Safety, Changsha, China
摘要:
The effects of heat shock (HT), 1-methylcyclopropene (1-MCP), or their combination (HT+1-MCP) on the quality of fresh jujube fruits during cold storage were studied. Among them, HT showed the best preservation effect on jujube fruits, which was more effective than others in inhibiting the increase of red index, decay incidence, and weight loss and delaying the decrease of firmness, soluble solids content (SSC), titratable acidity (TA), and ascorbic acid (AsA) content. Besides, it could delay the degradation rate of the cell wall to maintain the integrity of cell membrane, and keep the high activity of active oxygen scavenging enzymes. During cold storage, malondialdehyde (MDA) content and relative electrolyte leakage (REL) of the HT group were significantly lower than those of the control group, 1-MCP, and HT+1-MCP group (p<.05), while superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities were significantly higher than those of other groups (p<.05). It was concluded that the postharvest HT treatment could effectively delay the senescence and decay of jujube fruits. PRACTICAL APPLICATIONS: Jujube fruits have high nutritional value used for food and medicine. However, they are not tolerant to storage after harvest, resulting in high economic losses. Therefore, it is of great significance to find a suitable method to maintain the quality of jujube fruits. Our results revealed the effect of HT, 1-MCP, and their combination on the quality maintenance of jujube fruits, and found that HT could effectively maintain the quality of them, which could be used as an effective method for keeping jujube fruits fresh.
期刊:
Frontiers in Veterinary Science,2021年8:773912 ISSN:2297-1769
通讯作者:
Li, Man-Xiang;Xiao, Chao-Ting
作者机构:
[Ren, Jie; Fan, Fang-Cheng; Li, Man-Xiang; Ge, Meng; Zhao, Dun; Xie, Yi-Lin; Song, Xiao-Qin] Hunan Agr Univ, Coll Vet Med, Changsha, Peoples R China.;[Li, Man-Xiang; Ge, Meng] Hunan Agr Univ, Coll Vet Med, Hunan Engn Technol Res Ctr Vet Drugs, Changsha, Peoples R China.;[Xiao, Chao-Ting] Hunan Univ, Inst Pathogen Biol & Immunol, Coll Biol, Changsha, Peoples R China.
通讯机构:
[Li, Man-Xiang; Xiao, Chao-Ting] H;Hunan Agr Univ, Coll Vet Med, Changsha, Peoples R China.;Hunan Agr Univ, Coll Vet Med, Hunan Engn Technol Res Ctr Vet Drugs, Changsha, Peoples R China.;Hunan Univ, Inst Pathogen Biol & Immunol, Coll Biol, Changsha, Peoples R China.
关键词:
only plain text;China;complete genome;genetic divergence;PCV3;Prevalence
摘要:
Porcine circovirus type 3 (PCV3), a virus belonging to the Circoviridae family, is considered to be associated with respiratory and neurological signs, cardiac and multisystemic inflammation, reproductive failure, and porcine dermatitis and nephropathy syndrome-like disease in pigs (Sus scrofa). In this study, epidemiological and serological investigations of PCV3 in clinically healthy pigs from different regions of China were performed. Overall, 42.87% (1,101/2,568) of pigs were positive for PCV3 Cap antibody via indirect enzyme-linked immunosorbent assay, with a higher prevalence of PCV3 in multiparous sows (62.22%, 881/1,416) and fattening pigs (28.96%, 159/549) than in suckling piglets (8.96%, 32/357) and nursery pigs (11.79%, 29/246). Of the 2,568 samples, 255 were further tested for PCV3 DNA using real-time polymerase chain reaction, and 63.14% of these were positive, with nearly half having <10 virus copies. The PCV3 DNA and antibody positivity rates were high in the pig serum samples; however, the virus titers and antibody levels were both low, indicating that the humoral immune response of PCV3-infected pigs was weak or lagging, and persistent or repeated infections could occur. Additionally, the complete genomes of 23 PCV3 strains were sequenced and analyzed, which showed nucleotide identities of 98.5~100.0%, 98.6~100.0%, and 99.2~100.0% in the complete genome, open reading frame (ORF)2, and ORF1 sequences, respectively, and amino acid identities of 96.7~100.0% and 99.3~100.0% in the capsid and replicase proteins, respectively. Phylogenetic analysis based on ORF2 nucleotide sequences indicated that the PCV3 strains obtained in the present study could be classified into three sub-clades, with most strains clustered into clade 3c, indicating that PCV3c is the dominant subtype in the regions of China investigated. In general, the present study revealed a high prevalence and high genetic divergence of PCV3 among Chinese pig herds, and indicated that the potential effect of PCV3 on the pig industry may be a concern.
作者:
Su, Jing;Xu, Kai;Li, Zirong;Hu, Yuan;Hu, Zhongli;...
期刊:
Scientific Reports,2021年11(1) ISSN:2045-2322
通讯作者:
Li, Lanzhi
作者机构:
[Xu, Kai; Li, Lanzhi; Su, Jing; Hu, Yuan; Li, Zirong] Hunan Agr Univ, Hunan Engn & Technol Res Ctr Agr Big Data Anal &, Changsha 410128, Peoples R China.;[Hu, Zhongli] Wuhan Univ, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China.;[Zheng, Xingfei] Hubei Acad Agr Sci, Food Crop Inst, Hubei Key Lab Food Crop Germplasm & Genet Improve, Wuhan 430064, Peoples R China.;[Song, Shufeng] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China.;[Tang, Zhonghai] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.
通讯机构:
[Li, Lanzhi] H;Hunan Engineering & Technology Research Center for Agricultural Big Data Analysis & Decision-Making, Hunan Agricultural University, Changsha, China
摘要:
Rice yield per plant has a complex genetic architecture, which is mainly determined by its three component traits: the number of grains per panicle (GPP), kilo-grain weight (KGW), and tillers per plant (TP). Exploring ideotype breeding based on selection for genetically less complex component traits is an alternative route for further improving rice production. To understand the genetic basis of the relationship between rice yield and component traits, we investigated the four traits of two rice hybrid populations (575 + 1495 F1) in different environments and conducted meta-analyses of genome-wide association study (meta-GWAS). In total, 3589 significant loci for three components traits were detected, while only 3 loci for yield were detected. It indicated that rice yield is mainly controlled by minor-effect loci and hardly to be identified. Selecting quantitative trait locus/gene affected component traits to further enhance yield is recommended. Mendelian randomization design is adopted to investigate the genetic effects of loci on yield through component traits and estimate the genetic relationship between rice yield and its component traits by these loci. The loci for GPP or TP mainly had a positive genetic effect on yield, but the loci for KGW with different direction effects (positive effect or negative effect). Additionally, TP (Beta = 1.865) has a greater effect on yield than KGW (Beta = 1.016) and GPP (Beta = 0.086). Five significant loci for component traits that had an indirect effect on yield were identified. Pyramiding superior alleles of the five loci revealed improved yield. A combination of direct and indirect effects may better contribute to the yield potential of rice. Our findings provided a rationale for using component traits as indirect indices to enhanced rice yield, which will be helpful for further understanding the genetic basis of yield and provide valuable information for improving rice yield potential.
作者机构:
[Jan, Habib U.; Guan, Chunyun; Guan, Mei; Xiong, Xinghua; Min, Yao; Huang, Luyao; He, Xin; Qian, Lunwen; Hua, Wei] Hunan Agr Univ, Collaborat Innovat Ctr Grain & Oil Crops South Ch, Changsha 410128, Peoples R China.;[Qian, Wei] Southwest Univ, Coll Hort & Landscape Architecture, Chongqing 400715, Peoples R China.;[Hua, Wei] Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr, Oil Crops Res Inst, Wuhan 430062, Peoples R China.;[Snowdon, Rod J.; Schiessl, Sarah] Justus Liebig Univ, IFZ Res Ctr Biosyst Land Use & Nutr, Dept Plant Breeding, Heinrich Buff Ring 26-32, D-35392 Giessen, Germany.
关键词:
Brassica napus;Coexpression network;Flowering time;Genome-wide association analysis;Haplotype
摘要:
Flowering is an important turning point from vegetative growth to reproductive growth, and vernalization is an essential condition for the flowering of annual winter plants. To investigate the genetic architecture of flowering time in rapeseed, we used the 60 K Brassica Infinium SNP array to perform a genome-wide analysis of haplotype blocks associated with flowering time in 203 Chinese semi-winter rapeseed inbred lines. Twenty-one haplotype regions carrying one or more candidate genes showed a significant association with flowering time. Interestingly, we detected a SNP (Bn-scaff_22728_1-p285715) located in exon 3 of the BnVIN3-C03 gene that showed a significant association with flowering time on chromosome C03. Based on the SNP alleles A and G, two groups of accessions with early and late flowering time phenotypes were selected, respectively, and PCR amplification and gene expression analysis were combined to reveal the structural variation of the BnVIN3-C03 gene that affected flowering time. Moreover, we found that BnVIN3-C03 inhibited the expression of BnFLC-A02, BnFLC-A03.1, BnFLC-A10 and BnFLC-C03.1, thus modulating the flowering time of Brassica napus. This result provides insight into the genetic improvement of flowering time in B. napus.
摘要:
BACKGROUND: Gelsemium elegans (G. elegans) is a flowering plant of the Loganiaceae family, which had been used in traditional Chinese herb medicine for many years for the treatment of rheumatoid pain, neuropathic pain, spasticity, skin ulcers, anxiety and cancer. Acute toxicity of the plant severely limits the application and development of G. elegans; however, long-term toxicity of exposure to G. elegans has not been illuminated. PURPOSE: This study is a comprehensive observation of the effects of long-term exposure (21 days at 70 mg/kg) to G. elegans in rats. METHODS AND RESULTS: The histopathological examination showed only a mild glial cell proliferation in the brain, and no lesions were observed in other organs. No abnormal changes in the biochemical parameters were observed that would have significant effects. The identification and analysis of absorbed natural ingredients showed that the active ingredients of the G. elegans could distribute to various tissues, and six compounds were identified in the brain, suggesting that they could cross the blood-brain barrier. Based on the intestinal content metabolomics, the tryptophan (Trp) biosynthesis, bile acid synthesis and bile secretion pathways have attracted our attention. Plasma metabolomic results showed that uric acid (UA) was significantly increased. The results of the brain metabolomic tests showed that the level of pyridoxal (PL) was decreased; considering the expression levels of the related enzymes, it was hypothesized that the level of pyridoxal 5'-phosphate (PLP) was decreased. PLP was important for the regulation of the neuronal γ-aminobutyric acid (GABA)/glutamate (Glu) interconversion and therefore neuronal excitability. The data of the study suggested that toxic reaction caused by G. elegans was due to a disruption of the balance of the neurotransmitter GABA/Glu transformation. CONCLUSIONS: Overall, G. elegans did not cause significant toxic reaction in the rats after long-term exposure. The results were significant for the future clinical applications of G. elegans and suggested that G. elegans could be potentially developed as a drug. The study provided a scientific basis for investigation of the mechanisms of toxicity and detoxification.
作者机构:
[Meng, Delong; Tan, Ge; Gu, Yabing; Yin, Huaqun] Cent South Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.;[Zhou, Zhicheng; Peng, Shuguang; Liu, Yongjun] Tobacco Res Inst Hunan Prov, Changsha 410004, Peoples R China.;[Li, Juan; Liu, Yongjun] Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China.;[Tao, Jiemeng] CNTC, Zhengzhou Tobacco Res Inst, China Tobacco Gene Res Ctr, Zhengzhou 450001, Peoples R China.;[Yang, Sheng] Cent South Univ, Sch Energy Sci & Engn, Changsha 410083, Peoples R China.
通讯机构:
[Yongjun Liu; Zhicheng Zhou] T;Tobacco Research Institute of Hunan Province, Changsha, 410004, China<&wdkj&>College of Agronomy, Hunan Agricultural University, Changsha, 410128, China<&wdkj&>Tobacco Research Institute of Hunan Province, Changsha, 410004, China
关键词:
community structure;continuous cropping obstacle;molecular ecology networks (MENs);soil bacterial;soil property
摘要:
Continuous cropping has become the most common system in intensive, modern agricultural production; however, obstacles often appear in continuous cropping patterns after a few years of use. There have been several studies about the impacts of continuous cropping on soil microbial, but few about differences between soil experiencing continuous cropping obstacles and those where such obstacles had been resisted. Here, after ten or twenty years of continuous tobacco cropping, we collected soil samples investigating discrepancies in soil property and bacterial community between soils experiencing continuous cropping obstacles and soils where the obstacles were resisted providing insight into preventing and controlling continuous cropping obstacles. Results showed that soil organic matter (SOM), available phosphorus (AP), total nitrogen (TN), nitrate-N (NO(3)(-)-N), and bacterial diversity of samples where continuous cropping obstacles had been resisted were significantly higher than those where continuous cropping obstacles were present. Besides, SOM, AP, TN, and Ammonium-N (NH(4)(+)-N) had a considerable effect on the bacterial community. Among all variables, NH(4)(+)-N explained the largest proportion of bacterial community variation. Molecular ecological networks were used to putatively identify keystone taxa, including Acidobacteria Gp1, Acidobacteria Gp2, Acidobacteria Gp16, and WPS-1_genera_incertae_sedis. Their relative abundance significantly changed between the two conditions. Overall, our results indicate that decreases in soil nutrient content and bacterial diversity, and significant changes in some keystone taxa abundances may be important factors leading to increased soil-borne diseases and reduced tobacco production potential or quality. Thus, during agricultural production, we could regulate stability of the soil-crop-microbial ecological system via crop rotation, intercropping, or the use of specialized bio-fertilizers and soil conditioners to mitigate continuous cropping obstacles.
通讯作者:
Yongji Qin<&wdkj&>Sheng Dai<&wdkj&>Xijun Liu
作者机构:
[Luo, Jun; Liu, Shuai; Lai, Shuhua; Qin, Yongji; Qi, Defeng; Qiu, Yuan; Liu, Xijun] Tianjin Univ Technol, Sch Mat Sci & Engn, Inst New Energy Mat & Low Carbon Technol, Tianjin 300384, Peoples R China.;[Luo, Jun; Liu, Shuai; Lai, Shuhua; Qin, Yongji; Qi, Defeng; Qiu, Yuan; Liu, Xijun] Tianjin Univ Technol, Sch Mat Sci & Engn, Tianjin Key Lab Photoelect Mat & Devices, Tianjin 300384, Peoples R China.;[Chen, Honghui] Hunan Agr Univ, Coll Chem & Mat Sci, Changsha 410128, Peoples R China.;[Dai, Sheng] East China Univ Sci & Technol, Sch Chemist & Mol Engn, Inst Fine Chem, Key Lab Adv Mat & Feringa Nobel Prize Scientist J, Shanghai 200237, Peoples R China.;[Zhang, Shusheng] Zhengzhou Univ, Coll Chem, Zhengzhou 450000, Peoples R China.
通讯机构:
[Yongji Qin; Xijun Liu] I;[Sheng Dai] K;Key Laboratory for Advanced Materials and Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China<&wdkj&>Institute for New Energy Materials and Low-Carbon Technologies and Tianjin Key Lab for Photoelectric Materials & Devices, School of Materials Science and Engineering, Tianjin University of Technology, Tianjin 300384, China<&wdkj&>Institute for New Energy Materials and Low-Carbon Technologies and Tianjin Key Lab for Photoelectric Materials & Devices, School of Materials Science and Engineering, Tianjin University of Technology, Tianjin 300384, China<&wdkj&>Key Laboratory of Civil Aviation Thermal Hazards Prevention and Emergency Response, Civil Aviation University of China, Tianjin 300300, China
摘要:
Overall hydrazine splitting (OHzS) is a promising alternative for overall water splitting (OWS) towards hydrogen production due to the anodic hydrazine oxidation reaction (HzOR) involving less electrons and faster kinetics than the oxygen evolution reaction (OER). However, it still remains a challenge to synthesize bifunctional electrocatalysts for the hydrogen evolution reaction (HER) and HzOR with low overpotentials. Herein, we represent a N/S co-doped hollow carbon sphere loaded with Rh nanoparticles (Rh-NS-HCS) and demonstrate its dual appealing activities for both the HER and HzOR. Notably, the obtained Rh-NS-HCS shows superiorities for hydrogen production in OHzS with a cell voltage of 0.11 V to reach a current density of 10 mA cm(-2) when it serves as both a cathodic and anodic electrocatalyst. These performances can be attributed to the intrinsic nature of the Rh nanoparticles and N/S co-doped hollow structured carbon matrix, which could provide easy access to active sites, excellent conductivity and stability, thus making energy-efficient hydrogen production possible.
摘要:
Softening is one of the main factors affecting market value and consumer preferences for jujubes, and it was closely related to the modification and depolymerization of pectin. Changes in characteristics of three pectins (water-soluble pectin (WSP), sodium carbonate-soluble pectin (SSP) and chelate-soluble pectin (CSP)), including their contents, degree of methylesterification (DM), neutral sugar compositions, the molecular weight (M(w) ) distributions and nanostructures, from two jujube fruits cv Dongzao (DZ) and Jinsixiaozao (JS) during cold storage were assessed. The results showed that variation in pectin characteristics during cold storage was similar between DZ and JS. The reduction of firmness corresponded to a conversion of water-insoluble pectin to WSP during cold storage. DM of WSP presented an increase trend in the late storage. Rhamnose (Rha), arabinose (Ara) and glucose (Glc) were the crucial compositions in three pectins, and most neutral sugar compositions in three pectins first increased and then decreased during cold storage. Changes in the ratio of (galactose (Gal)+Ara)/Rha and Ara/Gal represented that the branch chains of rhamnogalacturonan-I in three pectins depolymerized after storage. The high M(w) in WSP and SSP of jujubes were solubilized and extensively depolymerized into pectin with lower M(w) after storage. AFM images showed an increase in short chains and branch structures of three pectins after storage. Overall, three pectins in DZ and JS depolymerized and solubilized during cold storage. WSP and SSP were more contributed to the softening of jujubes compared to CSP, and they played the critical role for regulating the softening of jujube fruits during cold storage. PRACTICAL APPLICATION: Softening is one of the main factors affecting market value and consumer preferences for jujubes, and it was closely related to the modification and depolymerization of pectin. Changes in characteristics of three pectins (WSP, SSP, CSP), including their contents, degree of methylesterification, neutral sugar compositions, the molecular weight distributions and nanostructures, from two jujube fruits cv Dongzao (DZ) and Jinsixiaozao (JS) during cold storage were assessed. Three pectins in DZ and JS depolymerized and solubilized during cold storage. WSP and SSP were more contributed to the softening of jujubes compared to CSP, and they played the critical role for regulating the softening of jujube fruits during cold storage. This study would elucidate the mechanism of jujube softening and help to regulate the postharvest quality during cold storage.