作者：
Singh, Rudra P.*;Dilworth, Andrea D.;Ao, Xiaoping;Singh, Mathuresh;Baranwal, Virendra K.
期刊：
European Journal of Plant Pathology,2009年124(4):691-694 ISSN：0929-1873
通讯作者：
Singh, Rudra P.
作者机构：
[Singh, Rudra P.; Dilworth, Andrea D.] Agr & Agri Food Canada, Potato Res Ctr, Fredericton, NB E3B 4Z7, Canada.;[Ao, Xiaoping] Hunan Agr Univ, Fac Hort & Landscape, Changsha 410128, Hunan, Peoples R China.;[Singh, Mathuresh] Agr Certificat Serv, Fredericton, NB E3B 8B7, Canada.;[Baranwal, Virendra K.] Indian Agr Res Inst, Div Plant Pathol, Plant Virol Unit, New Delhi 110012, India.;[Singh, Rudra P.] Agr & Agri Food Canada, Potato Res Ctr, 850 Lincoln Rd, Fredericton, NB E3B 4Z7, Canada.
通讯机构：
[Singh, Rudra P.] A;Agr & Agri Food Canada, Potato Res Ctr, 850 Lincoln Rd, Fredericton, NB E3B 4Z7, Canada.
关键词：
Ornamental plants;Seed transmission;Spread of viroids
摘要：
Surveys of Impatiens and Verbena species in local nurseries in Fredericton, Canada and Verbena species in New Delhi, India showed widespread infection of Citrus exocortis viroid (CEVd) in vegetatively-propagated and seed-grown plants. To determine viroid seed transmission, samples of eight varieties of Impatiens and 11 varieties of Verbena were obtained from four commercial sources. All 19 samples collected contained viroid infection irrespective of variety. The presence of viroid in non-germinated seed was 21%, while the transmission rate in seedlings was 66% in Impatiens walleriana in 2006. Following 2 years of seed storage, the respective figures were 6% and 26%. Similarly, in Verbena x hybrida the presence of viroid in seed was 13% in 2006 with a seed-transmission rate in seedlings of 28%, while the respective figures after 2 years of storage were 5% and 45%.
摘要：
Sweet orange (Citrus sinensis) represents an important group of Citrus fruit; however, the identification of sweet orange cultivars during vegetative growth can be difficult. A study on the genetic identification of sweet orange cultivars may be significant for the sweet orange nursery industry, for cultivar-rights protection, and is important for the genetic evaluation and conservation of these orange cultivars. In this study, amplified fragment length polymorphism (AFLP) markers were used to genotype 57 sweet orange cultivars. Ten PCR primer pairs generated 629 unique AFLP bands, with a size range of 50-500 bp. Seventy-four bands (11.8%) were polymorphic. On average, each primer pair produced 62.9 fragments, with 7.4 polymorphic fragments. A dendrogram of the 57 sweet orange cultivars was constructed based on an UPGMA analysis using Jaccard's coefficients of similarity. This provided a clear comparison of the genetic variation between cultivars and an ability to identify them. From Jaccard's coefficients, 56 of the 57 cultivars examined were genetically close, with coefficient values ≥ 0.985. 'Variegated Navel' was less closelyrelated, with a much lower coefficient value (0.94). Among the 57 cultivars, 28 sub-groups, some consisting of only one cultivar, could be separated by their AFLP fingerprints. Compared to ISSR and SSR markers, AFLP seemed to be the preferential marker technique for the identification of sweet orange cultivars.
摘要：
The F-box protein CORONATINE INSENSITIVE1 (COI1) plays a central role in jasmonate (JA) signaling and is required for all JA responses in Arabidopsis (Arabidopsis thaliana). To dissect JA signal transduction, we isolated the partially suppressing coi1 (psc1) mutant, which partially suppressed coi1 insensitivity to JA inhibition of root growth. The psc1 mutant partially restored JA sensitivity in coi1-2 background and displayed JA hypersensitivity in wild-type COI1 background. Genetic mapping, sequence analysis, and complementation tests revealed that psc1 is a leaky mutation of DWARF4 (DWF4) that encodes a key enzyme in brassinosteroid (BR) biosynthesis. Physiological analysis showed that an application of exogenous BR eliminated the partial restoration of JA sensitivity by psc1 in coi1-2 background and the JA hypersensitivity of psc1 in wild-type COI1 background. Exogenous BR also attenuated JA inhibition of root growth in the wild type. In addition, the expression of DWF4 was inhibited by JA, and this inhibition was dependent on COI1. These results indicate that (1) BR is involved in JA signaling and negatively regulates JA inhibition of root growth, and (2) the DWF4 is down-regulated by JA and is located downstream of COI1 in the JA-signaling pathway.
作者机构：
[Wan, Qiang; Zou, Bingsuo; Hu, Song; Zeng, Ruosheng; Zhang, Tingting; Peng, Zhiwei] Hunan Univ, State Key Lab CSBC, Changsha 410082, Hunan, Peoples R China.;[Wan, Qiang; Zou, Bingsuo; Hu, Song; Zeng, Ruosheng; Zhang, Tingting; Peng, Zhiwei] Hunan Univ, Micronano Ctr, Changsha 410082, Hunan, Peoples R China.;[Zeng, Ruosheng] Univ Arkansas, Dept Chem & Biochem, Fayetteville, AR 72701 USA.;[Zhang, Tingting] Hunan Agr Univ, Inst Plant Source Utilizat, Changsha 410128, Hunan, Peoples R China.;[Liu, Jincheng] S China Univ Technol, Inst Polymer Optoelect Mat & Devices, Guangzhou 510640, Guangdong, Peoples R China.
通讯机构：
[Wan, Qiang] H;Hunan Univ, State Key Lab CSBC, Changsha 410082, Hunan, Peoples R China.
摘要：
In this paper, we report a two-step aqueous synthesis of highly luminescent CdTe/CdSe core/shell quantum dots (QDs) via a simple method. The emission range of the CdTe/CdSe QDs can be tuned from 510 to 640 nm by controlling the thickness of the CdSe shell. Accordingly, the photoluminescence quantum yield (PL QY) of CdTe/CdSe QDs with an optimized thickness of the CdSe shell can reach up to 40%. The structures and compositions of the core/shell QDs were characterized by transmission electron microscopy, x-ray diffraction, and x-ray photoelectron spectroscopy experiments, and their formation mechanism is discussed. Furthermore, folate conjugated CdTe/CdSe QDs in Hela cells were assessed with a fluorescence microscope. The results show that folate conjugated CdTe/CdSe QDs could enter tumor cells efficiently.
作者机构：
[Distefano, Gaetano; Gentile, Alessandra; La Malfa, Stefano] Univ Catania, Dipartimento OrtoFloroArboricoltura & Tecnol Agro, I-95123 Catania, Italy.;[Vitale, Alessandro] Univ Catania, Dipartimento Sci & Tecnol Fitosanit, I-95123 Catania, Italy.;[Lorito, Matteo] Univ Naples Federico 2, Dipartimento Arboricoltura & Patol Vegetale, I-80055 Portici, NA, Italy.;[Deng, Ziniu] Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China.;[Gentile, Alessandra] Univ Catania, Dipartimento OrtoFloroArboricoltura & Tecnol Agro, Via Valdisavoia 5, I-95123 Catania, Italy.
通讯机构：
[Gentile, Alessandra] U;Univ Catania, Dipartimento OrtoFloroArboricoltura & Tecnol Agro, Via Valdisavoia 5, I-95123 Catania, Italy.
关键词：
Citrus limon;Defence response modification;Disease resistance assays;Real time PCR
摘要：
Constitutive over-expression of antifungal genes from microorganisms involved in plant defence mechanisms represents a promising strategy for conferring genetic resistance against a broad range of plant pathogenic fungi. In the present work, two transgenic lemon clones with the chit42 gene from Trichoderma harzianum were tested for resistance to fungal disease and expression level of defence-related genes was evaluated. Different resistance-related processes, such as production of reactive oxygen species (ROS), systemic acquired resistance (SAR) and induced systemic resistance (ISR), were monitored in transgenic and wild type lemon clones inoculated with Botrytis cinerea, the causal agent of grey mould in citrus. Expression of genes that encode gluthatione peroxidase (GPX), a producer of ROS, chitinases, glucanases (SAR), PAL, HPL, and AOS (ISR) was measured by quantitative PCR during the first 24 h after leaf inoculation. Leaves of transgenic lemon plants inoculated with B. cinerea showed significantly less lesion development than wild type leaves. Tissues from detached leaves of different transgenic lemon clones showed a significant correlation between resistance and transgene expression. On the other hand, the over-expression of the transgenic fungal gene enhanced by two-three folds transcript levels of genes associated with enhanced ROS production and ISR establishment, while the expression of native chitinase and glucanase genes involved in SAR was down-regulated.
摘要：
The transcription factor WRKY70 was previously reported to be a common component in salicylic acid (SA) and jasmonate (JA) mediated signal pathways in Arabidopsis. Here, we present that the inactivation of the WRKY70 gene in wrky70-1 mutant does not alter the responses of both JA and SA, and that wrky70 mutation is unable to restore the coi1 mutant in JA responses. However, overexpression of WRKY70 reduces JA responses such as expression of JA-induced genes and JA-inhibitory root growth, and activates expression of SA-inducible PR1. These data indicate that the WRKY70 is important but not indispensable for JA and SA signaling, and that other regulators may display the redundant role with WRKY70 in modulation of JA and SA responses in Arabidopsis. Furthermore, we showed that JA inhibits expression of WRKY70 and PR1 by both COI1-dependent and COI1-independent pathways.
关键词：
Amino acid digestibility;Cysteamine;Goat;Site and extent of digestion
摘要：
Abstract Tan, Z.L., Tang, S.X., Liao, Y.P., Zhou, C.S., Jiang, H.L., Sun, Z.H., Tayo, G.O., Xiao, W.J. and Chen, F.H. 2008. Effect of dietary supply of cysteamine on sites of digestion, small intestinal amino acid disappearance in growing goats. J. Appl. Anim. Res., 34: 71–76. Four Liuyang Black growing goats were used to examine the effect of dietary supply of cysteamine (CS) on feed intake, site and extent of digestion and apparent intestinal amino acid (AA) digestibility in a 4x4 Latin square design. The goats were weighed initially at 11.8 kg (SD 1.6), fitted with the permanent ruminal, duodenal and ileal fistulae and fed ad libitum in individual pens. Four treatments consisted of a basal diet only (control) and dietary supply of CS at levels of 20, 40 and 60 mg/kg dry matter, respectively, to the basal diet. The experimental period lasted for 14 d for each treatment. Dietary supply of CS had no effect (P>0.05) on intake of nutrients. Ruminal digestion of ADF and NDF decreased (P=0.01) on higher level of CS supplementation. The small intestinal digestibilities of alanine, methionine, threonine and valine decreased (P<0.05) with the inclusion of CS in the diets. The results imply that CS supplementation did not improve the digestibility and absorption of nutrients in the gastro-intestinal tract of goats.
