期刊：
Journal of Medicinal Plants Research,2011年5(14):3020-3027 ISSN：1996-0875
通讯作者：
Liang, Yizeng
作者机构：
[Liang, Yizeng; Tang, Zhonghai] Cent S Univ, Res Ctr Modernizat Chinese Tradit & Herbal Drug M, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Shi, Guorong; Yang, Hua; Qin, Jingping; Tang, Zhonghai] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Xu, Xiaona] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liang, Yizeng] C;Cent S Univ, Res Ctr Modernizat Chinese Tradit & Herbal Drug M, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
摘要：
The resveratrol contained in mulberry leaves has manifold health care functions. In the experiment, resveratrol was extracted from mulberry leaf using a reflux extraction method of the organic solvent, then resveratrol was purified by silica gel column chromatography and determined by high performance liquid chromatography after mulberry leaf was extracted, filtered, and vacuum concentrated. The optimum extraction conditions were as follows: material quality concentration: 1:13, extraction temperature: 60 degrees C, extraction time: 105 min, and concentration of ethanol: 80%. At these conditions, the yield of resveratrol was 0.0225%. Through the purity tests, mix chloroform and methanol in the ratio of 10:1 as the eluting solvent, the purified resveratrol by the silica gel column chromatography can be advanced from 0.8 to 99.3%.
期刊：
Proceedings of 2011 International Conference on Electronics and Optoelectronics,2011年1:V1-351-V1-355
作者机构：
[Hong-ling LEI] Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, Enshi, China;[Yong-yao WU] College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha, China
会议名称：
2011 International Conference on Electronics and Optoelectronics(2011电子学与光电子学国际会议 ICEOE 2011)
会议时间：
2011-07-29
会议地点：
大连
会议论文集名称：
2011 International Conference on Electronics and Optoelectronics(2011电子学与光电子学国际会议 ICEOE 2011)论文集
摘要：
To determine the physiological impact of selenium on the root of Cardamine sp., a Se-hyperaccumulator at Enshi, the plant was cultured in complete nutrient solution containing 0,15,30,45,60,75, or 90 mg·L-1 Na2SeO3, and the selenium content and the antioxidant activity in root were investigated. The results showed that Se addition can significantly increase selenium content, with the maximal level of 726.78 ug/g at Se concentration of 60 mg/L. The enzymatic activity of GSH-Px(glutathione peroxidase),CAT(catalase), POD(peroxidase) enhanced at Se addition level of 30-90 mg/L, with the highest activity at 60-75 mg/L Se concentration. The significant increase of enzymatic activity for SOD(superoxide dismutase) than control only occurred in the Se concentration of 75 mg/L. When Se concentration was 15-90 mg/L, there was only minimal MDA (malondialdehyde) production in root, and there were significant differences comparing to the control. Therefore, when Cardamine sp. is cultured in the complete nutrient solution, the antioxidant enzyme activity in root can be promoted and its ability for lipid peroxidation can be improved at 30-75mg/L of Se concentration.
摘要：
43 strains of cellulose-degradation fungi were isolated from the Rhizophora stylosa rhizospheric soil samples with 0-20 cm depth collected from Hongsha River of Sanya,Hainan.In order to further study
