期刊：
Trends in Food Science & Technology,2025年:104976 ISSN：0924-2244
通讯作者：
Zheng Wang
作者机构：
College of Bioscience and Biotechnology, Hunan Agricultural University, 41018, Changsha, China;[Mingchen Liu; Siyi Zhu] Chinese Medicinal Materials Breeding Innovation Center, Yuelushan Laboratory, 410128, Changsha, China;[Yicheng Li] Fine Biotechnological R&D Center, 510700, Guangzhou, China;[Jiwei Zhou] Independent researcher, Campbell, 95008, CA, USA;[Liangliang Liu] Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, 410205, Changsha, China
通讯机构：
[Zheng Wang] C;College of Bioscience and Biotechnology, Hunan Agricultural University, 41018, Changsha, China<&wdkj&>Chinese Medicinal Materials Breeding Innovation Center, Yuelushan Laboratory, 410128, Changsha, China
摘要：
Background Obesity represents a critical global health challenge, with appetite regulation serving as a cornerstone of effective weight management. Emerging evidence underscores the potential of exogenous peptides derived from food protein hydrolysates to modulate appetite-regulating neurons in the hypothalamus by influencing the secretion of key intestinal hormones.
Obesity represents a critical global health challenge, with appetite regulation serving as a cornerstone of effective weight management. Emerging evidence underscores the potential of exogenous peptides derived from food protein hydrolysates to modulate appetite-regulating neurons in the hypothalamus by influencing the secretion of key intestinal hormones.
Scope and approach This review discusses the fundamental pathways through which the hypothalamus governs appetite, highlighting the promising role of plant-derived protein hydrolysates and peptides in appetite regulation. The objective is to provide a comprehensive and up-to-date overview of how plant-derived these bioactive compounds regulate appetite.
This review discusses the fundamental pathways through which the hypothalamus governs appetite, highlighting the promising role of plant-derived protein hydrolysates and peptides in appetite regulation. The objective is to provide a comprehensive and up-to-date overview of how plant-derived these bioactive compounds regulate appetite.
Key findings and conclusions Plant-derived protein hydrolysates and peptides have demonstrated the ability to regulate the secretion and signaling of key gut hormones, which in turn influence appetite through the vagus nerve. This modulation presents promising applications in weight management and the prevention of chronic diseases associated with obesity. While challenges such as complex molecular mechanisms, high production costs, low bioavailability, instability, regulatory hurdles, and insufficient clinical translation data persist, plant-derived peptides stand out for their exceptional sustainability compared to animal- and microbiota-derived peptides. Future research should prioritize the optimization of production processes, enhancement of stability and safety, and the execution of rigorous clinical trials to establish their therapeutic efficacy. Through continuous innovation, plant-derived peptides hold great potential to become an indispensable component of functional foods and therapeutic interventions, offering a novel and highly promising solution for addressing obesity prevention and treatment in the food and healthcare industries.
Plant-derived protein hydrolysates and peptides have demonstrated the ability to regulate the secretion and signaling of key gut hormones, which in turn influence appetite through the vagus nerve. This modulation presents promising applications in weight management and the prevention of chronic diseases associated with obesity. While challenges such as complex molecular mechanisms, high production costs, low bioavailability, instability, regulatory hurdles, and insufficient clinical translation data persist, plant-derived peptides stand out for their exceptional sustainability compared to animal- and microbiota-derived peptides. Future research should prioritize the optimization of production processes, enhancement of stability and safety, and the execution of rigorous clinical trials to establish their therapeutic efficacy. Through continuous innovation, plant-derived peptides hold great potential to become an indispensable component of functional foods and therapeutic interventions, offering a novel and highly promising solution for addressing obesity prevention and treatment in the food and healthcare industries.
作者机构：
[Yuqing Tian; Chaochao Lv; Xiaolin Zhang; Hang Shan; Leyi Ni] State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China;[Yuqing Tian] Hubei Key Laboratory of Regional Development and Environmental Response, Faculty of Resource and Environment, Hubei University, Wuhan, 430062, China;[Hui Fu] Ecology Department, College of Resources & Environments, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Hunan Agricultural University, Changsha, 410128, China;[Qingchuan Chou] State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. chouqc@ihb.ac.cn;[Xuwei Deng] Donghu Experimental Station of Lake Ecosystem, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China
通讯机构：
[Qingchuan Chou; Te Cao] S;State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China<&wdkj&>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China
摘要：
Submerged macrophytes play a crucial role in lake ecosystems, and their survival is dependent upon their ability to cope with variable environmental stress. Therefore, studying the plastic response of submerged macrophytes' resource allocation and functional traits to the environment may provide insights helpful for ecological restoration practices. In September 2021, a field survey was conducted in the Erhai Lake, where samples of Ottelia acuminata, and functional traits and biomass allocation in relation to water depth were measured. The study found that O. acuminata exhibited large intraspecific variations to adapt to environmental stress, and the average intraspecific variation was 55.86%. In the current environment, this study suggested that the optimal growth depth for O. acuminata is moderate water depth (1-2m). The results of allometric growth analysis showed that the resource allocation of O. acuminata responded to the water depth with the synergistic effect of leaf area and shoot height. In shallow water, the resource allocation of O. acuminata was mainly used for leaf area growth, while in deep water, the increase of shoot height was mainly used to cope with stress. This research will provide us useful information for the ecological restoration and protection of this endemic and endangered submerged macrophyte.
摘要：
Chronic stress can result in various conditions, including psychological disorders, neurodegenerative diseases, and accelerated brain aging. Gut dysbiosis potentially contributes to stress-related brain disorders in individuals with chronic stress. However, the causal relationship and key factors between gut dysbiosis and brain disorders in chronic stress remain elusive, particularly under non-sterile conditions. Here, using a repeated restraint stress (RRS) rat model, we show that sequential transplantation of the cecal contents of different RRS stages to normal rats reproduced RRS-induced core phenotypes, including abnormal behaviors, increased peripheral blood corticosterone and inflammatory cytokines, and a unique gut microbial phenotype. This core phenotypic development was effectively inhibited with probiotic supplement. The RRS-induced unique gut microbial phenotypes at the genus level were positively or negatively associated with the levels of 20 plasma metabolites, including vitamin B6 metabolites 4-pyridoxic acid and 4-pyridoxate. Vitamin B6 supplement during RRS alleviated weight loss, abnormal behaviors, peripheral inflammation, and neuroinflammation, but did not affect the peripheral corticosterone levels in chronic stressed rats. Dampening inflammatory signaling via knocking out caspase 11 or caspase 1 inhibitor abolished RRS-induced abnormal behaviors and peripheral and neuroinflammation but did not decrease peripheral corticosterone in mice. These findings show that gut dysbiosis-induced vitamin B6 metabolism disorder is a new non-hypothalamic-pituitary-adrenal axis mechanism of chronic stress-related brain disorders. Both probiotics and vitamin B6 supplement have potential to be developed as therapeutic strategies for preventing and/or treating chronic stress-related illness.
期刊：
International Journal of Molecular Sciences,2025年26(5)
作者机构：
[Yi, Yuhang; Lv, Chenghao; Zhang, Yi; Liu, Xin; Zhou, Xixin] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;[Chen, Shiyun; Qin, Si; Fan, Wei] College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China;[Lv, Chenghao] Xiangya School of Basic Medical Sciences, Central South University, Changsha 410013, China
摘要：
The primary active compound in vine tea is dihydromyricetin (DMY), which has a longstanding history as a dietary supplement and traditional ethnic medicine. However, the precise molecular mechanism by which vine tea dihydromyricetin extract (VDMY) regulates glucolipid metabolic disorder remains unclear. In this study, we first assessed the effect of VDMY on various physiological parameters in db/db mice, followed by RNA sequencing (RNA-seq) to identify key signaling pathways affected by VDMY in liver tissues. We also examined the impact of VDMY on the liver's TLR4/MyD88/NF-κB and FOXO1 pathways using Western blotting. Our results showed that VDMY significantly reduced fasting blood glucose (FBG), total cholesterol (TC), triglycerides (TGs), and low-density lipoprotein cholesterol (LDL-C), while increasing high-density lipoprotein cholesterol (HDL-C) levels. Additionally, VDMY enhanced the liver's antioxidant capacity by upregulating superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH), while lowering malondialdehyde (MDA), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), thus alleviating liver damage. RNA-seq analysis further revealed that VDMY influenced multiple biological processes, including transcription, glycolysis, gluconeogenesis, and redox reactions, suggesting that its effects may be mediated through the TLR4/MyD88/NF-κB and FOXO1 pathways. Additionally, Western blot analysis revealed that VDMY effectively downregulated the expressions of TLR4, MyD88, NF-κB, and FOXO1 proteins in the liver of db/db mice, indicating that VDMY could target these pathways to intervene glucolipid metabolism dysfunction.
关键词：
Magnetic solid phase microextraction;Ni(3)(HITP)(2)@Fe(3)O(4)@rGO;Separation of chirality;Strigolactones stereoisomers;Supercritical fluid chromatography mass spectrometry
摘要：
Strigolactones (SLs) are novel plant hormones that play crucial roles in regulating plant growth and development. The stereoisomers of SLs often exhibit distinct biological activities, making their chiral separation and analysis essential for understanding their regulatory roles in plant branching. This study focused on the chiral separation and characterization of SL stereoisomers, including GR24 (synthetic) and strigol (natural). A magnetic nanocomposite (Ni₃(HITP)₂@Fe₃O₄@rGO) was synthesized for SL extraction, combined with SFC-MS/MS for precise separation and quantification. The method exhibited excellent linearity (0.2–50 ng/mL), minimal matrix effects (1.64–2.17 %), and low detection limits (1.93–3.76 pg/mL). Recoveries ranged from 86.90 % to 106.37 % with RSDs of 2.56 %–13.54 %. Under nitrogen and phosphorus stress, only (+)-strigol was detected in the roots of Oryza sativa L. , suggesting its regulatory role under stress conditions. This reliable method provides robust support for exploring the physiological roles of SL stereoisomers in plants, shedding light on their functions in stress response and development.
Strigolactones (SLs) are novel plant hormones that play crucial roles in regulating plant growth and development. The stereoisomers of SLs often exhibit distinct biological activities, making their chiral separation and analysis essential for understanding their regulatory roles in plant branching. This study focused on the chiral separation and characterization of SL stereoisomers, including GR24 (synthetic) and strigol (natural). A magnetic nanocomposite (Ni₃(HITP)₂@Fe₃O₄@rGO) was synthesized for SL extraction, combined with SFC-MS/MS for precise separation and quantification. The method exhibited excellent linearity (0.2–50 ng/mL), minimal matrix effects (1.64–2.17 %), and low detection limits (1.93–3.76 pg/mL). Recoveries ranged from 86.90 % to 106.37 % with RSDs of 2.56 %–13.54 %. Under nitrogen and phosphorus stress, only (+)-strigol was detected in the roots of Oryza sativa L. , suggesting its regulatory role under stress conditions. This reliable method provides robust support for exploring the physiological roles of SL stereoisomers in plants, shedding light on their functions in stress response and development.
作者：
Su, Yujie;Zhao, Qianwen;Chang, Cuihua;Li, Junhua;Guo, Lulu;...
期刊：
Foods,2025年14(5) ISSN：2304-8158
作者机构：
[Chang, Cuihua; Guo, Lulu; Yang, Yanjun; Hu, Shende; Su, Yujie; Li, Junhua; Gu, Luping; Zhao, Qianwen] State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi 214122, China;[Su, Yujie] Hunan Jiapin Jiawei Technology Development Group Co., Ltd, Hunan Engineering & Technology Research Center for Food Flavors and Flavorings, Jinshi 415400, China;[Huang, Zijian] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
摘要：
Ovotransferrin (OVT) is very rich in nutritional value and possesses a variety of biological activities. However, there is a lack of suitable OVT extraction methods that are simple and suitable for large-scale production. For this reason, this study explored a new method of ovalbumin OVT extraction based on mesophilic treatment. The effects of different heat treatment conditions on the physicochemical properties and bioactivities of the prepared OVT and their influence mechanisms were investigated. The results showed that OVT could be efficiently extracted from egg white by moderate heat treatment. Based on single factor experiments, response surface methodology was used to determine the effects of heat treatment time, temperature and pH on the extraction rate of OVT. The yield was 93.65 ± 0.53% under the optimal extraction conditions (62.5 °C, 75 min, pH 8). SDS-PAGE and FT-IR showed that changes in the influencing factors during heating had different effects on OVT. In addition, different extraction parameters had different effects on the iron-binding and antioxidant capacities of OVT. This study provides a fast and efficient preparation method for OVT from egg white, which lays the foundation for the wide application of OVT.
摘要：
Overuse of glyphosate, the most widespread herbicide used in agricultural areas around the world, causes it to accumulate in soil and water, posing a serious threat to the agricultural environment, crop growth and food safety. It is of vital significance to develop effective strategies to achieve rapid monitoring and management of glyphosate. However, previously documented methods have rarely been applied to simultaneously detect and remove glyphosate in water environments. Here, we have created a novel magnetic nanocomposite Iron-oxide/polydopamine/graphene-oxide/copper-oxide (Fe3O4/PDA/GO/CuO) that integrated the dual functions of detection and removal, enabling the colorimetric detection and adsorption of glyphosate. As a colorimetric probe, Fe3O4/PDA/GO/CuO exhibited excellent sensing performance with broad detection range (0.05–1 mg/L and 5–110 mg/L), low detection limit (0.028 mg/L), and good selectivity. Simultaneously, it realized a rapid and sensitive visual analysis of glyphosate on the test strips by RGB color. As an adsorbent, Fe3O4/PDA/GO/CuO obtained effective adsorption and rapid separation of glyphosate in water solution. Moreover, as an attempt, we explored the potential of Fe3O4/PDA/GO/CuO for crop remediation by removing glyphosate-contaminated water. This work opens up a new idea for the integrated strategy of glyphosate detection and removal in water environments and also demonstrates its enormous potential for rapid monitoring and management of herbicide.
Overuse of glyphosate, the most widespread herbicide used in agricultural areas around the world, causes it to accumulate in soil and water, posing a serious threat to the agricultural environment, crop growth and food safety. It is of vital significance to develop effective strategies to achieve rapid monitoring and management of glyphosate. However, previously documented methods have rarely been applied to simultaneously detect and remove glyphosate in water environments. Here, we have created a novel magnetic nanocomposite Iron-oxide/polydopamine/graphene-oxide/copper-oxide (Fe3O4/PDA/GO/CuO) that integrated the dual functions of detection and removal, enabling the colorimetric detection and adsorption of glyphosate. As a colorimetric probe, Fe3O4/PDA/GO/CuO exhibited excellent sensing performance with broad detection range (0.05–1 mg/L and 5–110 mg/L), low detection limit (0.028 mg/L), and good selectivity. Simultaneously, it realized a rapid and sensitive visual analysis of glyphosate on the test strips by RGB color. As an adsorbent, Fe3O4/PDA/GO/CuO obtained effective adsorption and rapid separation of glyphosate in water solution. Moreover, as an attempt, we explored the potential of Fe3O4/PDA/GO/CuO for crop remediation by removing glyphosate-contaminated water. This work opens up a new idea for the integrated strategy of glyphosate detection and removal in water environments and also demonstrates its enormous potential for rapid monitoring and management of herbicide.
期刊：
Resources, Conservation and Recycling,2025年215:108114 ISSN：0921-3449
通讯作者：
Xu, Y
作者机构：
[Shi, Wenjun] Bank Beijing, Bank Beijing Postdoctoral Res Stn, Beijing 100033, Peoples R China.;[Xu, Yi] China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China.;[Tan, Tiansu] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Fang, Yanru] Chinese Res Inst Environm Sci, Beijing 100012, Peoples R China.;[Liu, Xiaotong] Inst Resources & Environm, Int Ctr Bamboo & Rattan, Key Lab Natl Forestry & Grassland Adm, Beijing 100102, Peoples R China.
通讯机构：
[Xu, Y ] C;China Agr Univ, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China.
关键词：
Published works (article and dissertation) related to straw return-induced changes in grain yield of rice;maize and wheat in China were collected from the Google Scholar;Web of Science and China National Knowledge Infrastructure (CNKI) before January 2023. The main keywords used for relevant publications research were "crop straw or residue or stubble or stover";"return or retention or input or incorporation or mulch" and "yield or productivity". The literature items were then selected
摘要：
Effective management of crop straw is crucial for sustainable food production, renewable energy transition and climate change mitigation. However, accurately estimating reasonable removal rates to enhance grain yield and transition to bioenergy remains challenging. Through nationwide systematic surveys and meta-analysis, reasonable straw removal rates of 45 % for rice, 19 % for wheat, and 42 % for maize were identified in China. The potential bioenergy production derived from these reasonably removed straws ranges from 23 to 100 Mtce in 2022. The combined adjustment of straw utilization and fossil fuel offset through bioenergy leads to a reduction in greenhouse gas (GHG) emissions by 218 to 309 Mt in 2022. Particularly, maize straw emerges as a preferred feedstock for bioenergy and climate mitigation due to its highest bioenergy production and GHG emissions reduction potential. These findings provide critical insights into optimizing agricultural residue management in China, guiding efforts toward food security and carbon neutrality goals.
Effective management of crop straw is crucial for sustainable food production, renewable energy transition and climate change mitigation. However, accurately estimating reasonable removal rates to enhance grain yield and transition to bioenergy remains challenging. Through nationwide systematic surveys and meta-analysis, reasonable straw removal rates of 45 % for rice, 19 % for wheat, and 42 % for maize were identified in China. The potential bioenergy production derived from these reasonably removed straws ranges from 23 to 100 Mtce in 2022. The combined adjustment of straw utilization and fossil fuel offset through bioenergy leads to a reduction in greenhouse gas (GHG) emissions by 218 to 309 Mt in 2022. Particularly, maize straw emerges as a preferred feedstock for bioenergy and climate mitigation due to its highest bioenergy production and GHG emissions reduction potential. These findings provide critical insights into optimizing agricultural residue management in China, guiding efforts toward food security and carbon neutrality goals.
通讯机构：
[Jin, J ; Li, YY ] S;Sun Yat Sen Univ, Affiliated Hosp 3, Ctr Neuroimmunol & Hlth Longev, Guangzhou 510630, Peoples R China.;Zhejiang Univ, Sir Run Run Shaw Hosp, Coll Med, Dept Gastroenterol, Hangzhou 310016, Peoples R China.;Zhejiang Univ, Life Sci Inst, MOE Key Lab Biosyst Homeostasis & Protect, Zhejiang Prov Key Lab Canc Mol Cell Biol, Hangzhou 310058, Zhejiang, Peoples R China.;Southeast Univ, Jiangsu Prov High Tech Key Lab Biomed Res, Key Lab Dev Genes & Human Dis, Minist Educ,Inst Life Sci, Nanjing 210096, Peoples R China.
关键词：
AdorA2B;Lactobacillus murinus;irritable bowel syndrome with diarrhea;spermidine;stress;type I interferon;xanthine
摘要：
Irritable bowel syndrome with diarrhea (IBS-D) is a common and chronic gastrointestinal disorder that is characterized by abdominal discomfort and occasional diarrhea. The pathogenesis of IBS-D is thought to be related to a combination of factors, including psychological stress, abnormal muscle contractions, and inflammation and disorder of the gut microbiome. However, there is still a lack of comprehensive analysis of the logical regulatory correlation among these factors. In this study, we found that stress induced hyperproduction of xanthine and altered the abundance and metabolic characteristics of Lactobacillus murinus in the gut. Lactobacillus murinus -derived spermidine suppressed the basal expression of type I interferon (IFN)-α in plasmacytoid dendritic cells by inhibiting the K63-linked polyubiquitination of TRAF3. The reduction in IFN-α unrestricted the contractile function of colonic smooth muscle cells, resulting in an increase in bowel movement. Our findings provided a theoretical basis for the pathological mechanism of, and new drug targets for, stress-exposed IBS-D.
Irritable bowel syndrome with diarrhea (IBS-D) is a common and chronic gastrointestinal disorder that is characterized by abdominal discomfort and occasional diarrhea. The pathogenesis of IBS-D is thought to be related to a combination of factors, including psychological stress, abnormal muscle contractions, and inflammation and disorder of the gut microbiome. However, there is still a lack of comprehensive analysis of the logical regulatory correlation among these factors. In this study, we found that stress induced hyperproduction of xanthine and altered the abundance and metabolic characteristics of Lactobacillus murinus in the gut. Lactobacillus murinus -derived spermidine suppressed the basal expression of type I interferon (IFN)-α in plasmacytoid dendritic cells by inhibiting the K63-linked polyubiquitination of TRAF3. The reduction in IFN-α unrestricted the contractile function of colonic smooth muscle cells, resulting in an increase in bowel movement. Our findings provided a theoretical basis for the pathological mechanism of, and new drug targets for, stress-exposed IBS-D.
摘要：
The widespread application of biogas projects generates substantial amounts of waste fermentation residue. Further treatment of fermentation residues facilitates resource utilization, ensures safe disposal, and is anticipated to enhance the economic returns of biogas projects. Herein, catalytic liquefaction of pig manure fermentation residue to produce biocrude oil was investigated using various alkaline catalysts at 340 ℃ with ethanol as the solvent. Biocrude oils were analyzed by elemental analysis, gas chromatography-mass spectrometry (GC–MS), thermogravimetric analysis, and kinetic analysis. The maximum biocrude oil yield (45.24 wt%) was obtained with the KOH catalyst. Additionally, the biocrude oil produced by the catalysis of CaO exhibited the maximum higher heating value at 44.18 MJ/kg. GC–MS results showed that KOH and K 2 CO 3 considerably increased the content of phenols and hydrocarbons in the biocrude while reducing nitrogenous compounds. All alkaline catalysts effectively reduced the activation energy of biocrude oil compared to biocrude oil without catalyst. The maximum reduction in activation energy (18.73 %) was achieved with the addition of Na 2 CO 3 . More importantly, adding CaO not only increased the yield and higher heating value of biocrude oil but also reduced nitrogenous compounds and activation energy, improving the overall yield and quality. Overall, this work provides an effective and promising method to convert pig manure fermentation residue into green high-quality biocrude oil, simultaneously providing an economical and environmentally friendly waste management strategy for the fermentation industry.
The widespread application of biogas projects generates substantial amounts of waste fermentation residue. Further treatment of fermentation residues facilitates resource utilization, ensures safe disposal, and is anticipated to enhance the economic returns of biogas projects. Herein, catalytic liquefaction of pig manure fermentation residue to produce biocrude oil was investigated using various alkaline catalysts at 340 ℃ with ethanol as the solvent. Biocrude oils were analyzed by elemental analysis, gas chromatography-mass spectrometry (GC–MS), thermogravimetric analysis, and kinetic analysis. The maximum biocrude oil yield (45.24 wt%) was obtained with the KOH catalyst. Additionally, the biocrude oil produced by the catalysis of CaO exhibited the maximum higher heating value at 44.18 MJ/kg. GC–MS results showed that KOH and K 2 CO 3 considerably increased the content of phenols and hydrocarbons in the biocrude while reducing nitrogenous compounds. All alkaline catalysts effectively reduced the activation energy of biocrude oil compared to biocrude oil without catalyst. The maximum reduction in activation energy (18.73 %) was achieved with the addition of Na 2 CO 3 . More importantly, adding CaO not only increased the yield and higher heating value of biocrude oil but also reduced nitrogenous compounds and activation energy, improving the overall yield and quality. Overall, this work provides an effective and promising method to convert pig manure fermentation residue into green high-quality biocrude oil, simultaneously providing an economical and environmentally friendly waste management strategy for the fermentation industry.
摘要：
Background Based on the low volatility and refractory nature of Tetracycline (TC), excessive use leads to its continuous accumulation in water environments, posing serious risks to the ecological environment and human health. Although a very limited number of nanomaterials capable of simultaneously detecting and removing TC have been fabricated, they generally exist issues associated with a single detection signal (“on” or “off”) or low adsorption rates with low adsorption capacities. As a result, it is crucial to develop a reliable technique to achieve ratiometric detection as well as rapid and efficient removal of TC.
Based on the low volatility and refractory nature of Tetracycline (TC), excessive use leads to its continuous accumulation in water environments, posing serious risks to the ecological environment and human health. Although a very limited number of nanomaterials capable of simultaneously detecting and removing TC have been fabricated, they generally exist issues associated with a single detection signal (“on” or “off”) or low adsorption rates with low adsorption capacities. As a result, it is crucial to develop a reliable technique to achieve ratiometric detection as well as rapid and efficient removal of TC.
Results Herein, a novel Zn (II) Functionalized magnetic covalent organic framework (Fe 3 O 4 @COF@Zn) was created. As the role of a fluorescent probe, it had excellent characteristics of ratiometric (F 529 /F 436 ), ultrafast response (1 min), and ultra-low detection limit (16 nM). As the role of an adsorbent, it demonstrated a high capacity of adsorption (414.94 mg/g) in the pH-neutral range, fast kinetics (10 min), desirable regeneration capability, and convenient magnetic separation. By theoretical and experimental analysis, the detection and adsorption mechanism for TC was systematically revealed. Moreover, as an attempt, Fe 3 O 4 @COF@Zn showed it potential for crop remediation by adsorbing TC-contaminated water.
Herein, a novel Zn (II) Functionalized magnetic covalent organic framework (Fe 3 O 4 @COF@Zn) was created. As the role of a fluorescent probe, it had excellent characteristics of ratiometric (F 529 /F 436 ), ultrafast response (1 min), and ultra-low detection limit (16 nM). As the role of an adsorbent, it demonstrated a high capacity of adsorption (414.94 mg/g) in the pH-neutral range, fast kinetics (10 min), desirable regeneration capability, and convenient magnetic separation. By theoretical and experimental analysis, the detection and adsorption mechanism for TC was systematically revealed. Moreover, as an attempt, Fe 3 O 4 @COF@Zn showed it potential for crop remediation by adsorbing TC-contaminated water.
Significance This work demonstrates the exceptional performance of Zn-functionalized fluorescent COF for ratiometric, ultrafast, sensitive detection as well as rapid and efficient removal of TC, thereby illustrating its significant potential for the rapid monitoring and treatment of TC contamination.
This work demonstrates the exceptional performance of Zn-functionalized fluorescent COF for ratiometric, ultrafast, sensitive detection as well as rapid and efficient removal of TC, thereby illustrating its significant potential for the rapid monitoring and treatment of TC contamination.
期刊：
Journal of Nanobiotechnology,2025年23(1):1-18 ISSN：1477-3155
通讯作者：
Guangfu Feng<&wdkj&>Aike Li<&wdkj&>Jun Fang
作者机构：
[Yanquan Fei; Lixiang Wang; Hongting Cai; Huipeng Liu; Zizi Ding; Yuewen Huang] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China;[Guangfu Feng] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China. GuangfuFeng@hunau.edu.cn;[Aike Li] Feed Research Institute/Grain Quality & Nutrition Institute, Key Laboratory of Grain and Oil Biotechnology of National Food and Strategic Reserves Administration, Academy of National Food and Strategic Reserves Administration, Beijing, 100037, China. lak@AGS.AC.CN;[Jun Fang] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China. fangjun1973@hunau.edu.cn
通讯机构：
[Guangfu Feng; Jun Fang] C;[Aike Li] F;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China<&wdkj&>Feed Research Institute/Grain Quality & Nutrition Institute, Key Laboratory of Grain and Oil Biotechnology of National Food and Strategic Reserves Administration, Academy of National Food and Strategic Reserves Administration, Beijing, China<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China
摘要：
The occurrence of inflammatory bowel disease (IBD) is relevant to impaired intestinal mucosal barrier and disordered gut microbiota, subsequently leading to excessive production of reactive oxygen species (ROS) and elevated levels of inflammatory factors. Traditional therapies focus on inhibiting inflammation, but the vast majority involve non-targeted systemic administration, whose long-term use may result in potential side effects. Oral microbial therapy has exhibited great application prospects currently in IBD treatment; however, its progress has been slowed by issues with deficient bioavailability, poor targeting of colitis, and low therapeutic efficacy. Consequently, it is exceedingly desirable to develop a strategy by which probiotics can be endowed with additional anti-inflammatory and antioxidant properties, as well as enhanced targeting of the inflamed intestine. Herein, we present an innovative therapeutic strategy for encapsulating probiotic Bacillus coagulans spores with rosmarinic acid (RA) and silk fibroin (SF). Probiotics in spore morphology possessed strong gastrointestinal environmental resistance; RA alleviated oxidative damage by scavenging ROS and inhibited inflammatory responses; SF assisted probiotics release and colonize in the inflamed intestine. We demonstrated the therapeutic efficacy of probiotic composite materials in a colitis mouse model, which significantly alleviated a series of colitis symptoms, inhibited inflammatory cytokine storms, restored the balance of the gut microbiota, and downregulated inflammation-related signaling pathways. We are optimistic that the utilization of therapeutic nanocoating to modify probiotics will open up novel avenues for future microbial therapy targeting IBD.
摘要：
Accurate identification of tea leaf diseases is crucial for intelligent tea cultivation and monitoring. However, the complex environment of tea plantations-affected by weather variations and uneven lighting-poses significant challenges for building effective disease recognition models using raw field-captured images. To address this, we propose a method that combines two-stage image segmentation with an improved conditional generative adversarial network (IC-GAN). The two-stage segmentation approach, integrating graph cuts and support vector machines (SVM), effectively isolates disease regions from complex backgrounds. The IC-GAN augments the dataset by generating high-quality synthetic disease images for model training. Finally, an Inception Embedded Pooling Convolutional Neural Network (IDCNN) is developed for disease recognition. Experimental results demonstrate that the segmentation method improves recognition accuracy from 53.36% to 75.63%, while the IC-GAN increases the dataset size. The IDCNN achieves 97.66% accuracy, 97.36% recall, and a 96.98% F1 score across three types of tea diseases. Comparative evaluations on two additional datasets further confirm the method's robustness and accuracy, offering a practical solution to reduce tea production losses and improve quality.
摘要：
Osteoporosis is divided into primary and secondary types. Primary osteoporosis may result from estrogen deficiency in postmenopausal women, imbalanced bone remodeling in the elderly, or imbalanced adolescent-type bone development. Secondary osteoporosis can be caused by factors like long-term glucocorticoid treatment, chronic kidney disease (CKD), estrogen deprivation, oxidative stress, diabetes, and obesity. This review focuses on the therapeutic potential of soy isoflavones for osteoporosis. At the cellular level, soy isoflavones, as natural plant extracts and phytoestrogens, are crucial for osteoblastogenesis and differentiation, osteoclastogenesis, osteoclast mineralization, and bone marrow mesenchymal stromal cell differentiation. They also maintain calcium homeostasis by regulating extracellular calcium and vitamin D levels. In terms of oxidative stress, soy isoflavones mitigate it in the endoplasmic reticulum and mitochondria, thus regulating cellular senescence, autophagy, and bone remodeling processes. Moreover, soy isoflavones can relieve symptoms related to CKD and inhibit glucocorticoid secretion, which directly or indirectly benefits the treatment of osteoporosis. Overall, soy isoflavones have the potential to treat osteoporosis by enhancing bone health, regulating metabolism, and alleviating oxidative stress. Future research should explore the potential of soy isoflavones as phytoestrogens for treating osteoporosis. This exploration should focus on clarifying the safety, identifying potential side effects, determining the optimal dosage regimen, and developing strategies to mitigate any adverse reactions. In addition, further large-scale, multicenter human clinical trials are necessary to accurately evaluate the actual therapeutic effect of soy isoflavones on osteoporosis.
期刊：
Environmental Toxicology and Chemistry,2025年 ISSN：0730-7268
作者机构：
[Yang, Huilin; Wang, Zhuoman; Li, Zhongyuan; Gao, Cuimei; Huang, Siqi; Jiang, Zijian] College of Environment & Ecology, Hunan Agricultural University, Changsha, Hunan, 410128China;[Yang, Huilin; Wang, Zhuoman; Li, Zhongyuan; Gao, Cuimei; Huang, Siqi; Jiang, Zijian] Team of High Value Utilization of Crop Ecology, Yuelushan Laboratory, Changsha, Hunan, 410128China;[Yang, Huilin; Wang, Zhuoman; Li, Zhongyuan; Gao, Cuimei; Huang, Siqi; Jiang, Zijian] Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Changsha, Hunan, 410128China;[Liu, Jing] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, 410128China
关键词：
Arthropods in farmland;Ecotoxicological effects;Farmland pollution;Omics technology
摘要：
Arthropods, abundant in farmland, have unique biological traits that make them valuable for studying the ecotoxicological impacts of pollutants. Recent advancements in multi-omics technologies have enhanced their use in assessing pollution risks and understanding toxicity mechanisms. This paper reviews recent developments in applying omics technologies-genomics, transcriptomics, proteomics, metabolomics and meta-omics to ecotoxicological research on farmland arthropods. Agricultural arthropods manage genes and proteins like metallothioneins (MTs), antioxidant enzyme systems, heat shock proteins, cytochrome P450 (CYP450), carboxylesterases (CarEs), and glutathione S-transferases (GSTs), for detoxification and antioxidant purposes. They adjust amino acid, sugar, and lipid metabolism to counteract pollutant-induced energy drain and modify gut microbiota to aid in detoxification. This study advocates for enhanced analysis of compound pollution and emerging pollutants using multi-omics, especially meta-omics, to clarify the toxicological mechanisms underlying arthropod responses to these pollutants. Furthermore, it underscores the urgent need for subsequent gene function mining and validation to support biological control strategies and promote sustainable agricultural practices. The findings of this research provide significant insights into the toxicological impacts and mechanisms of pollutants within farmland ecosystems, thereby contributing to the preservation of arthropod diversity.
作者机构：
[Ye, Shunxing] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China;[Tang, Yuzhou] College of Landscape Architecture and Art Design, Hunan Agricultural University, Changsha, China
关键词：
Abiotic stress;C2H2-type zinc finger protein;Drought;TaZFP23;Wheat (Triticum aestivum L.)
摘要：
Wheat (Triticum aestivum L.) is an important food crop and one of the most important grains in the world. With the global climate change, wheat production is increasingly affected by abiotic stress, among which drought, salinity, and other factors have become the main abiotic stress factors restricting the efficient production of wheat. The C2H2-type zinc finger proteins are a common class of transcription factors in plants that play crucial roles in regulating plant growth and development as well as responses to stresses. In this study, the wheat C2H2-type zinc finger protein transcription factor TaZFP23 was cloned. Its full-length coding sequence was 720 bp encoding 239 amino acids. TaZFP23 is a typical C2H2-type zinc finger protein. It contains two C2H2 zinc finger domains and an EAR motif, without a transmembrane domain. Promoter cis-acting element analysis suggested that TaZFP23 might function in abiotic stress responses and plant hormone signal transduction. Subcellular localization and transcriptional activity assays indicated that TaZFP23 encoded a nuclear protein without self-activation activity. Overexpressing TaZFP23 in Arabidopsis thaliana showed that it negatively regulate d seed germination and plant growth under NaCl, mannitol, and ABA treatments. Additionally, TaZFP23 overexpression under NaCl and drought stress in Arabidopsis resulted in lower expression levels of several stress-related marker genes compared to those in wild-type plants. This research provides a foundation for further elucidating the functions of C2H2-type zinc finger protein genes and offers promising candidate genes for the development of stress-tolerant wheat cultivars.
作者：
Kai Teng;Wendi Lan;Guosu Lei;Hui Mao;Minghui Tian;...
期刊：
Current Microbiology,2025年82(5):1-15 ISSN：0343-8651
通讯作者：
Huaqun Yin<&wdkj&>Yunhua Xiao
作者机构：
[Kai Teng; Jingjing Ma; Guosu Lei; Wendi Lan] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China;[Hui Mao; Kai Teng; Minghui Tian; Jin Chao] Hunan Tobacco Company Xiangxi Autonomous Prefecture Corporation, Jishou, 416000, China;[Delong Meng] School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China;[Huaqun Yin] School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China. yinhuaqun_cs@sina.com;[Yunhua Xiao] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China. huazipiaoling.123@163.com
通讯机构：
[Huaqun Yin] S;[Yunhua Xiao] C;School of Minerals Processing and Bioengineering, Central South University, Changsha, China<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China<&wdkj&>Yuelushan Laboratory, Changsha, China
摘要：
Tobacco bacterial wilt (TBW), caused by Ralstonia solanacearum, significantly impacts tobacco yield and quality, leading to substantial economic losses. This study investigated the effects of the microbial agents JX (Pichia sp. J1 and Klebsiella oxytoca ZS4) on the soil properties, rhizospheric microbial community, tobacco agronomic traits, and TBW incidence through field experiments. Our results revealed that JX effectively suppressed TBW (relative control efficiency reached 85.18%) and promoted plant growth, enhancing agronomic traits by 9.58%-49.12%. The microbial agents JX was also attributed to improving soil properties (e.g., organic matter, total nitrogen, total phosphorus, and total potassium). Results of high-throughput sequencing revealed that JX not only affected the structure and diversity of tobacco rhizospheric bacterial/fungal community but also increased the relative abundances of critical microorganisms (e.g., Mesorhizobium, Pedosphaera, Nocardioides, Massilia, Burkholderia, and Purpureocilliu). Functional predictions showed the relative abundance of the pathways about some key secondary metabolisms (e.g., biosynthesis pathway of nicotinamide, phenylpropanoid, indole alkaloids, penicillin, cephalosporin, and novobiocin) and functional enzymes (e.g., catalase, ferroxidase, and tryptophan synthase) increased, which may also be critical factors in promoting tobacco growth and controlling TBW. These findings underscored the effectiveness of JX in both promoting tobacco growth and inhibiting TBW, supporting its practical application in tobacco cultivation.
摘要：
Proline biosynthesis and catabolism pathways are executed by powerful action of specific enzymes that are subjected to environmental fluctuations at the transcriptional level. Previous researches have demonstrated that osmotic stress-induced upstream events can affect the expression of proline metabolism-related genes, which results in adjustable free proline accumulation to protect plant cells from severe damage. Here, we mainly describe the mechanisms for how some key factors, such as transcription factors, ABA (abscisic acid), Ca 2+ , MAPK cascades, CK (cytokinin) and phospholipase, in a phosphorylated manner, vividly function in the transcriptional regulation of proline metabolism under osmotic stress. These mechanisms reveal that sustaining of proline homeostasis is an efficient way for plants to adapt to osmotic stress.
Proline biosynthesis and catabolism pathways are executed by powerful action of specific enzymes that are subjected to environmental fluctuations at the transcriptional level. Previous researches have demonstrated that osmotic stress-induced upstream events can affect the expression of proline metabolism-related genes, which results in adjustable free proline accumulation to protect plant cells from severe damage. Here, we mainly describe the mechanisms for how some key factors, such as transcription factors, ABA (abscisic acid), Ca 2+ , MAPK cascades, CK (cytokinin) and phospholipase, in a phosphorylated manner, vividly function in the transcriptional regulation of proline metabolism under osmotic stress. These mechanisms reveal that sustaining of proline homeostasis is an efficient way for plants to adapt to osmotic stress.
摘要：
Soil heavy metal pollution presents substantial risks to food security and human health. This study focused on the efficiency of plant growth-promoting fungus- Beauveria bassiana FE14 and Miscanthus floridulus on the synergistic remediation of soil Cd contamination. Results revealed that B. bassiana FE14 significantly enhanced the growth of M. floridulus , substantially decreased Cd content in soil by 79.39 %, and modified enzyme activities (superoxide dismutase, peroxidase, and catalase) to alleviate Cd-induced oxidative stress in plants, determined by the physical and chemical indicators and enzyme activities of soil and plant. Based on microbiome analysis, this study also found significant changes in the composition, structure, and molecular ecological network of endophytic bacterial communities in roots, but this study had little effect on the bacterial and fungal communities in rhizosphere soil. In addition, the key genera (including Sphingomonas , unclassified_Comamonadaceae, Massilia , Bradyrhizobium , and Paraglomus ) and key genes/enzymes (including cadC, zinc transporter, zinc and cadmium transporter, exoZ/Y/Z, catalase-peroxidase, superoxide dismutase, nitrite reductase, acid phosphatase, etc.) were involved in promoting plant growth and alleviating Cd stress. These findings revealed the potential of B. bassiana FE14 and M. floridulus working in synergy to enhance the phytoremediation efficiency of Cd-contaminated soils, thus presenting a promising approach for integrated plant-microbe remediation strategies.
Soil heavy metal pollution presents substantial risks to food security and human health. This study focused on the efficiency of plant growth-promoting fungus- Beauveria bassiana FE14 and Miscanthus floridulus on the synergistic remediation of soil Cd contamination. Results revealed that B. bassiana FE14 significantly enhanced the growth of M. floridulus , substantially decreased Cd content in soil by 79.39 %, and modified enzyme activities (superoxide dismutase, peroxidase, and catalase) to alleviate Cd-induced oxidative stress in plants, determined by the physical and chemical indicators and enzyme activities of soil and plant. Based on microbiome analysis, this study also found significant changes in the composition, structure, and molecular ecological network of endophytic bacterial communities in roots, but this study had little effect on the bacterial and fungal communities in rhizosphere soil. In addition, the key genera (including Sphingomonas , unclassified_Comamonadaceae, Massilia , Bradyrhizobium , and Paraglomus ) and key genes/enzymes (including cadC, zinc transporter, zinc and cadmium transporter, exoZ/Y/Z, catalase-peroxidase, superoxide dismutase, nitrite reductase, acid phosphatase, etc.) were involved in promoting plant growth and alleviating Cd stress. These findings revealed the potential of B. bassiana FE14 and M. floridulus working in synergy to enhance the phytoremediation efficiency of Cd-contaminated soils, thus presenting a promising approach for integrated plant-microbe remediation strategies.
摘要：
The antifungal targets of the new fungicide N-(naphthalen-1-yl)-phenazine-1-carboxamide (NNPCN) are still incomplete, limiting its application. To identify potential new targets of NNPCN and facilitate target hunting, a suite of techniques was employed to conduct experiments on Rhizoctonia solani. Nine potential targets were identified, exhibiting strong binding affinity to NNPCN, as indicated by binding free energies below -100.000 kJ/mol. Notably, pectin lyase, glycosyl hydrolase, fumarate transporter, and cytochrome monooxygenase showed exceptionally strong binding. The mRNA expression analysis revealed significant downregulation in certain target genes: E3 ubiquitin ligase (AG1IA_02506), aldehyde dehydrogenase (AG1IA_03762), fumarate transporter (AG1IA_03944), and pectin lyase (AG1IA_03046) decreased by 42%, 66%, 83%, and 69%, respectively, while other key genes were upregulated. Pectin lyase protein was obtained through prokaryotic expression at 0.4 mg/mL concentration. A novel thiobarbituric acid test system verified pectin lyase as a potential NNPCN target, with the enzyme activity multiple being only 0.169 after NNPCN treatment. These findings enhance our understanding of NNPCN's mode of action and could guide its improved application.
