作者机构：
[Luo, Shuangcheng] Hunan Agr Univ, Sch Econ, Changsha 410128, Peoples R China.;[Yuan, Yangli] Beijing Foreign Studies Univ, Sch Int Relationships & Diplomacy, Beijing 100089, Peoples R China.
通讯机构：
[Shuangcheng Luo] S;School of Economics, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Energy conservation and emission reduction are important ways to cope with global warming. An analysis of energy conservation and emission reduction from the perspective of network infrastructure construction provides an important perspective for the study of sustainable development. Based on the research sample of 263 cities in China from 2006 to 2019, and taking the policy of “Broadband China” as a quasi-natural experiment, this paper uses the double difference model to evaluate the impact of network infrastructure construction on energy conservation and emission reduction. The results show that (1) the construction of network infrastructure can significantly improve the energy utilization rate and reduce carbon emissions intensity, which helps to promote energy conservation and emission reduction. (2) From the perspective of a functional mechanism, on the one hand, network infrastructure construction affects energy conservation and emission reduction through micro-mechanisms such as green technology innovation and energy efficiency. On the other hand, network infrastructure construction also drives the development of the Internet and the digital economy, and promotes energy conservation and emission reduction through macro-mechanisms such as industrial structure and financial development. (3) The heterogeneity analysis shows that network infrastructure construction in non-resource-based cities, eastern regions and low-carbon cities has a greater impact on energy conservation and emission reduction. This study provides a new perspective for achieving low-carbon development goals.
作者机构：
[Zhang, Donglin; Zhang, Yifan; Yu, Xiaoying; Li, Yanlin; Xiong, Xingyao; Liu, Yang; Li, YL] Hunan Agr Univ, Coll Hort, Hunan Midsubtrop Qual Plant Breeding & Utilizat En, Engn Res,Ctr Hort Crop Germplasm Creat & New Varie, Changsha 410128, Peoples R China.;[Lin, Ling] Hunan Agr Univ, Sch Econ, Changsha 410128, Peoples R China.;[Xiong, Xingyao] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China.;[Li, Yanlin; Xiong, Xingyao; Li, YL] Kunpeng Inst Modern Agr, Foshan 528225, Peoples R China.;[Zhang, Donglin] Univ Georgia, Dept Hort, Athens, GA 30602 USA.
通讯机构：
[Yu, XY; Li, YL ] H;Hunan Agr Univ, Coll Hort, Hunan Midsubtrop Qual Plant Breeding & Utilizat En, Engn Res,Ctr Hort Crop Germplasm Creat & New Varie, Changsha 410128, Peoples R China.;Kunpeng Inst Modern Agr, Foshan 528225, Peoples R China.;Nanyang Technol Univ, Sch Biol Sci, 60 Nanyang Dr, Singapore 637551, Singapore.
关键词：
Loropetalumchinense var. rubrum;WRKY;expression pattern;genome-wide analysis;light quality
摘要：
The WRKY gene family plays important roles in plant growth and development, as well as in the responses to biotic and abiotic stresses. Loropetalum chinense var. rubrum has high ornamental and medicinal value. However, few WRKY genes have been reported in this plant, and their functions remain unknown. To explore the roles that the WRKY genes play in L. chinense var. rubrum, we identified and characterized 79 LcWRKYs through BLAST homology analysis and renamed them (as LcWRKY1-79) based on their distribution on the chromosomes of L. chinense var. rubrum. In this way, according to their structural characteristics and phylogenetic analysis, they were divided into three groups containing 16 (Group I), 52 (Group II), and 11 (Group III) WRKYs, respectively. LcWRKYs in the same group have similar motifs and gene structures; for instance, Motifs 1, 2, 3, 4, and 10 constitute the WRKY domain and zinc-finger structure. The LcWRKY promoter region contains light response elements (ACE, G-box), stress response elements (TC-rich repeats), hormone response elements (TATC-box, TCA-element), and MYB binding sites (MBS, MBSI). Synteny analysis of LcWRKYs allowed us to establish orthologous relationships among the WRKY gene families of Arabidopsis thaliana, Oryza sativa, Solanum lycopersicum L., Vitis vinifera L., Oryza sativa L., and Zea mays L.; furthermore, analysis of the transcriptomes of mature leaves and flowers from different cultivars demonstrated the cultivar-specific LcWRKY gene expression. The expression levels of certain LcWRKY genes also presented responsive changes from young to mature leaves, based on an analysis of the transcriptome in leaves at different developmental stages. White light treatment led to a significant decrease in the expression of LcWRKY6, 18, 24, 34, 36, 44, 48, 61, 62, and 77 and a significant increase in the expression of LcWRKY41, blue light treatment led to a significant decrease in the expression of LcWRKY18, 34, 50, and 77 and a significant increase in the expression of LcWRKY36 and 48. These results enable a better understanding of LcWRKYs, facilitating the further exploration of their genetic functions and the molecular breeding of L. chinense var. rubrum.
