Characterization of a novel thermo-stable lipase from endophyte Pseudomonas putida in Pistacia chinensis Bunge
作者：
Song, C.;Liu, Z.;Xie, Q.;Wang, H.;Huang, Y.;...
期刊：
Applied Biochemistry and Microbiology ,2017年53(5):524-532 ISSN：0003-6838
通讯作者：
Chen, D.
作者机构：
[Xie, Q.; Wang, H.; Ruan, Y.; Huang, Y.; Song, C.; Chen, D.] Hunan Agr Univ, Coll Biosci & Biotechnol, Dept Hunan Prov Plant Genet & Mol Biol, Key Lab Educ, Changsha 410128, Hunan, Peoples R China.;[Liu, Z.] Hunan Agr Univ, Coll Agr, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Chen, D.] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Dept Hunan Prov Plant Genet & Mol Biol, Key Lab Educ, Changsha 410128, Hunan, Peoples R China.
关键词：
Pseudomonas putida;alkaline lipase;thermostability;lipolytic enzyme;alpha/beta-hydrolase
摘要：
A novel lipolytic enzyme-producing endophytic strain PC2 was successfully isolated from the seeds of an ideal bioenergy plant Pistacia chinensis Bunge. Based on the analysis of morphology and 16S rRNA sequence, bacterial strain PC2 was identified as a subspecies of Pseudomonas putida, therefore named as P. putida PC2. Whole-genome sequencing showed PC2 contained a 1224-nucleotide lipase gene (named lip-PC2) predicted to encode a 407-amino-acid protein. Purified lipases from both the original PC2 strain and heterologously expressed Escherichia coli were nearly 50 kD with specific activity of 9.48 U/mL. LIP-PC2 displayed the maximal activity at 50°C or pH 8.0, and maintained above 80% relative activity in the range of from 40 to 60°C or pH in the range of from 6.0 to 8.0, indicating thermostable and alkaline properties. Enzyme activity was enhanced by Mg2+, Na+ and Mn2+, but strongly inhibited by Cu2+, Zn2+ Co2+, EDTA as well as organic solvents and surfactants. Additionally, the analysis of amino acid sequence and structure indicated that LIP-PC2 was a novel member belonging to family I.3 of bacterial lipolytic enzymes and its catalytic triad was consisted of Ser-200, Asp-342 and His-374. © 2017, Pleiades Publishing, Inc.
语种：
英文
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Conservation and diversification of polycomb repressive complex 2 (PRC2) proteins in the green lineage
作者：
Huang, Yong;Chen, Dong-Hong;Liu, Bo-Yu;Shen, Wen-Hui;Ruan, Ying*
期刊：
Briefings in Functional Genomics ,2017年16(2):106-119 ISSN：2041-2649
通讯作者：
Ruan, Ying
作者机构：
[Chen, Dong-Hong; Ruan, Ying; Huang, Yong; Liu, Bo-Yu; Shen, Wen-Hui] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
通讯机构：
[Ruan, Ying] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
关键词：
polycomb;PRC2;phylogenetic analysis;domain organization;evolution
摘要：
The polycomb group (PcG) proteins are key epigenetic regulators of gene expression in animals and plants. They act in multiprotein complexes, of which the best characterized is the polycomb repressive complex 2 (PRC2), which catalyses the trimethylation of histone H3 at lysine 27 (H3K27me3) at chromatin targets. In Arabidopsis thaliana, PRC2 proteins are involved in the regulation of diverse developmental processes, including cell fate determination, vegetative growth and development, flowering time control and embryogenesis. Here, we systematically analysed the evolutionary conservation and diversification of PRC2 components in lower and higher plants. We searched for and identified PRC2 homologues from the sequenced genomes of several green lineage species, from the unicellular green alga Ostreococcus lucimarinus to more complicated angiosperms. We found that some PRC2 core components, e.g. E(z), ESC/FIE and MSI/p55, are ancient and have multiplied coincidently with multicellular evolution. For one component, some members are newly formed, especially in the Cruciferae. During evolution, higher plants underwent copy number multiplication of various PRC2 components, which occurred independently for each component, without any obvious co-amplification of PRC2 members. Among the amplified members, usually one was well-conserved and the others were more diversified. Gene amplification occurred at different times for different PcG members during green lineage evolution. Certain PRC2 core components or members of them were highly conserved. Our study provides an insight into the evolutionary conservation and diversification of PcG proteins and may guide future functional characterization of these important epigenetic regulators in plants other than Arabidopsis. © The Author 2016.
语种：
英文
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A Dual-Promoter Gene Orchestrates the Sucrose-Coordinated Synthesis of Starch and Fructan in Barley
作者：
Jin, Yunkai;Fei, Mingliang;Rosenquist, Sara;Jin, Lu;Gohil, Suresh;...
期刊：
分子植物 ,2017年10(12):1556-1570 ISSN：1674-2052
通讯作者：
Liu, Chunlin;Sun, Chuanxin
作者机构：
[Ruan, Ying; Jin, Yunkai; Liu, Chunlin; Jin, Lu; Fei, Mingliang] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Olsson, Helena; Jin, Yunkai; Sun, Chuanxin; Rosenquist, Sara; Hoglund, Anna-Stina; Jin, Lu; Fei, Mingliang] Swedish Univ Agr Sci, Linnean Ctr Plant Biol, Uppsala BioCtr, Dept Plant Biol, POB 7080, S-75007 Uppsala, Sweden.;[Ruan, Ying; Fei, Mingliang] Hunan Agr Univ, Coll Biosci & Biotechnol, Dept Hunan Prov Plant Genet & Mol Biol, Key Lab Educ, Changsha 410128, Hunan, Peoples R China.;[Gohil, Suresh; Sandstrom, Corine] Swedish Univ Agr Sci, Uppsala BioCtr, Dept Chem & Biotechnol, POB 7015, S-75007 Uppsala, Sweden.;[Persson, Cecilia] Univ Gothenburg, Swedish NMR Ctr, Box 465, S-40530 Gothenburg, Sweden.
通讯机构：
[Liu, Chunlin] H;[Sun, Chuanxin] S;Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;Swedish Univ Agr Sci, Linnean Ctr Plant Biol, Uppsala BioCtr, Dept Plant Biol, POB 7080, S-75007 Uppsala, Sweden.
摘要：
Sequential carbohydrate synthesis is important for plant survival because it guarantees energy supplies for growth and development during plant ontogeny and reproduction. Starch and fructan are two important carbohydrates in many flowering plants and in human diets. Understanding this coordinated starch and fructan synthesis and unraveling how plants allocate photosynthates and prioritize different carbohydrate synthesis for survival could lead to improvements to cereals in agriculture for the purposes of greater food security and production quality. Here, we report a system from a single gene in barley employing two alternative promoters, one intronic/exonic, to generate two sequence-overlapping but functionally opposing transcription factors, in sensing sucrose, potentially via sucrose/glucose/fructose/trehalose 6-phosphate signaling. The system employs an autoregulatory mechanism in perceiving a sucrose-controlled trans activity on one promoter and orchestrating the coordinated starch and fructan synthesis by competitive transcription factor binding on the other promoter. As a case in point for the physiological roles of the system, we have demonstrated that this multitasking system can be exploited in breeding barley with tailored amounts of fructan to produce healthy food ingredients. The identification of an intron/exon-spanning promoter in a hosting gene, resulting in proteins with distinct functions, adds to the complexity of plant genomes.
语种：
英文
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甘蓝型油菜花粉母细胞减数分裂行为观察
作者：
李东昊;姜玲;阮颖
期刊：
湖南农业科学 ,2017年(5):1-3 ISSN：1006-060X
作者机构：
湖南农业大学生物科学技术学院,湖南长沙,410128;[李东昊; 阮颖; 姜玲] 湖南农业大学
关键词：
甘蓝型油菜;花粉母细胞;减数分裂
摘要：
以甘蓝型油菜"湘油15号"为材料,采用改良的卡宝品红染色液对其进行花粉母细胞减数分裂过程的细胞学观察.结果表明:当甘蓝型油菜花蕾长度小于1.5 mm时,花粉母细胞大多处于减数分裂时期;而花蕾长度介于1.5~2.5 mm时,花粉母细胞大多已发育为单核花粉粒;相较于碱性品红和Gimesa染色液,改良的卡宝品红染色液着色效果更佳,核质区分明显;甘蓝型油菜花粉母细胞在经历减数分裂Ⅰ和减数分裂Ⅱ后形成四分体,期间存在两次胞质分裂.
语种：
中文
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T-6b allocates more assimilation product for oil synthesis and less for polysaccharide synthesis during the seed development of Arabidopsis thaliana
作者：
Jin, Yunkai;Hu, Jia;Liu, Xun;Ruan, Ying;Sun, Chuanxin* ;...
期刊：
Biotechnology for Biofuels and Bioproducts ,2017年10(1):1-12 ISSN：2731-3654
通讯作者：
Liu, Chunlin;Sun, Chuanxin
作者机构：
[Hu, Jia; Ruan, Ying; Jin, Yunkai; Liu, Chunlin; Liu, Xun] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Jin, Yunkai; Sun, Chuanxin] Swedish Univ Agr Sci, Linnean Ctr Plant Biol, Uppsala BioCtr, Dept Plant Biol, POB 7080, SE-75007 Uppsala, Sweden.;[Hu, Jia; Ruan, Ying; Liu, Xun] Hunan Agr Univ, Dept Hunan Prov Plant Genet & Mol Biol, Coll Biosci & Biotechnol, Key Lab Educ, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Chunlin] H;[Sun, Chuanxin] S;Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;Swedish Univ Agr Sci, Linnean Ctr Plant Biol, Uppsala BioCtr, Dept Plant Biol, POB 7080, SE-75007 Uppsala, Sweden.
关键词：
T-6b;Endosperm expression;Starch;Mucilage;Lipid;Biosynthesis
摘要：
Background: As an Agrobacterium tumefaciens T-DNA oncogene, T-6b induces the development of tumors and the enation syndrome in vegetative tissues of transgenic plants. Most of these effects are related to increases in soluble sugar contents. To verify the potential roles of T-6b in the distribution of carbon in developing seeds, not in vegetative tissues, we fused an endosperm-specific promoter to the T-6b gene for expression in transgenic Arabidopsis thaliana plants. Results: The expression of T-6b in reproductive organs did not induce the development of the enation syndrome, and moreover, promoted endosperm expansion, which increased the total seed biomass by more than 10%. Additionally, T-6b also increased oil content in mature seeds by more than 10% accompanied with the decrease of starch and mucilage content at the same time. Conclusions: T-6b enhances seed biomass and helps oil biosynthesis but not polysaccharides in reproductive organs without disturbing vegetative growth and development. Our findings suggest T-6b may be very useful for increasing oil production in biodiesel plants. © 2017 The Author(s).
语种：
英文
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甘蓝型油菜BnaLCR23基因CRISPR-Cas9表达载体的构建及遗传转化
作者：
刘询;张斌;李浪;刘春林;阮颖
期刊：
分子植物育种 ,2017年15(8):3024-3029 ISSN：1672-416X
作者机构：
湖南农业大学生物科学技术学院, 长沙, 410128;湖南农业大学农学院, 长沙, 410128;[刘询; 张斌; 阮颖] 湖南农业大学生物科学技术学院, 长沙, 410128;[李浪; 刘春林] 湖南农业大学农学院, 长沙, 410128
关键词：
甘蓝型油菜;遗传转化
摘要：
BnaLCR23基因是甘蓝型油菜“湘油15号”种子不同发育时期抑制差减杂交文库中的一个功能未知基因,分析表明其功能可能与种子发育和油脂代谢相关,为了明确该基因的功能,本研究基于 CRISPR-Cas9基因编辑系统构建了该基因的敲除质粒。利用根癌农杆菌介导的油菜下胚轴遗传转化方法将敲除质粒转入甘蓝型油菜中,经潮霉素抗性筛选以及抗性苗基因组DNA的PCR检测,获得转基因油菜。转基因油菜材料的获得为深入研究BnaLCR23基因在甘蓝型油菜种子发育和储存物的积累过程中的功能提供了帮助。
语种：
中文
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植物多胺抗热机制及多胺转运蛋白的研究进展
作者：
欧阳家俊;宋春竹;陈东红;黄勇;阮颖
期刊：
分子植物育种 ,2017年15(8):3286-3294 ISSN：1672-416X
作者机构：
[欧阳家俊; 宋春竹; 陈东红; 黄勇; 阮颖] 湖南农业大学生物科学技术学院, 植物遗传与分子生物学湖南省教育厅重点实验室, 长沙, 410128
关键词：
多胺;多胺转运蛋白;LHR1保守性分析;高温胁迫
摘要：
多胺作为生理活性物质与植物耐热性关系紧密。多胺转运蛋白能够特异性介导多胺类物质跨膜运输。研究表明跨膜后的多胺类物质参与植物热胁迫早期反应中的分子调控过程,这对农作物耐高温及稳产高产有着重要意义。本研究对多胺合成途径、多胺作用机制、多胺的转运及多胺转运蛋白研究4个方面进行综述,为多胺及多胺转运蛋白的进一步研究和应用提供了理论依据。
语种：
中文
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The evolutionary landscape of PRC1 core components in green lineage
作者：
Chen, Dong-hong* ;Huang, Yong;Ruan, Ying* ;Shen, Wen-Hui
期刊：
Planta ,2016年243(4):825-846 ISSN：0032-0935
通讯作者：
Chen, Dong-hong;Ruan, Ying
作者机构：
[Chen, Dong-hong; Chen, DH; Ruan, Y; Ruan, Ying; Huang, Yong; Shen, Wen-Hui] Hunan Agr Univ, CNRS Fudan HUNAU Plant Epigenome Res, Int Associated Lab, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Shen, Wen-Hui] Univ Strasbourg, CNRS, Inst Biol Mol Plantes, 12 Rue Gen Zimmer, F-67084 Strasbourg, France.
通讯机构：
[Chen, DH; Ruan, Y] H;Hunan Agr Univ, CNRS Fudan HUNAU Plant Epigenome Res, Int Associated Lab, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
关键词：
PcG;PRC1;RING1;BMI1;LHP1
摘要：
Main conclusion: The origin and evolution of plant PRC1 core components. Polycomb repressive complex1 (PRC1) plays critical roles in epigenetic silencing of homeotic genes and determination of cell fate. Animal PRC1 has been well investigated for a long time, whereas plant PRC1 was just confirmed in recent years. It is enigmatic whether PRC1 core components in plants share a common ancestor with those in animals. We evaluated the origin of plant PRC1 RING-finger proteins (RING1 and BMI1) through comparing with the homologs in some representative unikonts and using BMI1- and RING1-like proteins as reciprocal outgroup, finding both PRC1 RING-finger proteins have the earliest origin in mosses, similar to LHP1. Additionally, the gene structure, copy number, and domain organization were analyzed to deeply understand the evolutionary history of plant PRC1 complex. In conclusion, PRC1 RING-finger proteins have independent origins in plants and animals, but convergent evolution might attribute to the conservation of PRC1 complex in plants and animals. Plant LHP1 as the homolog of non-PRC1 protein HP1 was recruited to fulfill the role of Pc counterpart. Gene duplication followed by functional divergence makes a great contribution to evolutionary progress of PRC1 in green plants. © 2016, Springer-Verlag Berlin Heidelberg.
语种：
英文
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Interplay of the histone methyltransferases SDG8 and SDG26 in the regulation of transcription and plant flowering and development
作者：
Liu, Boyu;Berr, Alexandre;Chang, Cheng;Liu, Chunlin;Shen, Wen-Hui* ;...
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS ,2016年1859(4):581-590 ISSN：1388-1981
通讯作者：
Ruan, Ying;Shen, Wen-Hui
作者机构：
[Ruan, Ying; Liu, Boyu; Chang, Cheng] Hunan Agr Univ, Dept Hunan Prov Plant Genet & Mol Biol, Coll Biosci & Biotechnol, Key Lab Educ, Changsha 410128, Hunan, Peoples R China.;[Ruan, Ying; Liu, Chunlin; Liu, Boyu; Chang, Cheng] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Berr, Alexandre; Shen, Wen-Hui] Univ Strasbourg, CNRS, IBMP, UPR2357, 12 Rue Gen Zimmer, F-67084 Strasbourg, France.
通讯机构：
[Ruan, Ying] H;[Shen, Wen-Hui] U;Hunan Agr Univ, Dept Hunan Prov Plant Genet & Mol Biol, Coll Biosci & Biotechnol, Key Lab Educ, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;Univ Strasbourg, CNRS, IBMP, UPR2357, 12 Rue Gen Zimmer, F-67084 Strasbourg, France.
关键词：
Arabidopsis thaliana;Chromatin remodeling;Flowering time control;Histone methylation;SET domain;Transcription regulation
摘要：
Histone methylations play fundamental roles in epigenetic regulation of transcription in many eukaryotes. In the model plant Arabidopsis thaliana as in human, a same histone lysine residue (e.g. H3K4 or H3K36) can be a potential target substrate for various different histone lysine methyltransferases (HKMTs). Using powerful genetic tool in Arabidopsis, we here investigate the interplay between two close HKMT homologues, SET DOMAIN GROUP 8 (SDG8) and SDG26, in regulating transcription and plant growth and development. We show that the sdg8 mutation is epistatic to the sdg26 one, leading to a sdg8 sdg26 double mutant exhibiting defects similar to sdg8 (reduced level of H3K36me3, increased level of H3K36me1, reduced plant body size, early flowering associated with reduced expression of FLC and MAFs and increased expression of FT and SOC1), but opposite to sdg26 (increased rosette size, late flowering associated with increased FLC and MAF5 expression and reduced FT and SOC1 expression). In parallel to the finding of the epistasis of SDG8, our study also unravels novel functions of SDG26 in H3K36me1 deposition and in the interplay with SDG8 in regulating the genome-wide gene expression. The implication of various HKMTs in establishing different forms of histone methylation and gene-context-specific chromatin modifications likely provides an advantageous mechanism for the regulation of transcription to cope with complex and plastic growth and developmental programs in plants and possibly also in other organisms. © 2016 Elsevier B.V.
语种：
英文
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江永香柚不同发育时期果皮分泌结构及油体的观察
作者：
蒋昊;桂茜;阮颖
期刊：
湖南农业科学 ,2016年(11):60-63 ISSN：1006-060X
作者机构：
湖南农业大学生物科学技术学院,湖南 长沙,410128;[蒋昊; 桂茜; 阮颖] 湖南农业大学
关键词：
江永香柚;冰冻切片;分泌结构;油体
摘要：
利用冰冻切片法和组织化学方法对江永香柚不同发育时期果实的果皮进行显微结构、分泌组织和油体的观察，结果发现：授粉后发育至70 d左右的江永香柚果实，其果皮为绿色，外果皮细胞内含大量叶绿体。发育70~150 d的果实其果皮逐渐由绿色转变为黄色，外果皮细胞内叶绿体分布逐渐减少。柚果实外果皮有分泌结构（精油腔）散布在外果皮中，精油腔由两个肾形细胞包围而成，精油腔空隙含有较多的油体。外果皮细胞内有黄色油体的分布，与同属的橙果实外果皮中相比，油体体积大，数量少。
语种：
中文
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以拟南芥为材料的发育生物学实验实践技术教改研究
作者：
黄勇;陈东红;阮颖
期刊：
现代农业科技 ,2016年(10):326-327 ISSN：1007-5739
作者机构：
湖南农业大学生物科学技术学院,湖南长沙,410128;[陈东红; 黄勇; 阮颖] 湖南农业大学
关键词：
发育生物学;实验实践;教改;拟南芥
摘要：
为提高高等农业院校发育生物学实验实践的教学质量,综述了发育生物学实验实践教学的改革成果,并初步探索了以拟南芥为材料的发育生物学实验实践教学的内容及实施方案,效果明显。
语种：
中文
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甘蓝型油菜BnaSOT12a基因的抗性作用研究
作者：
欧阳宁;金璐;阮颖;陈东红
期刊：
湖南农业大学学报(自然科学版) ,2016年42(3):251-255 ISSN：1007-1032
作者机构：
[欧阳宁; 金璐; 阮颖; 陈东红] 湖南农业大学生物科学技术学院, 湖南, 长沙, 410128
关键词：
甘蓝型油菜;拟南芥;BnaSOT12a基因;半定量PCR;植物抗盐;基因表达
摘要：
磺酸转移酶(SOT, EC 2.8.2.-)催化的磺酸化反应在植物的生长发育和植物抗逆过程中起着重要的作用。甘蓝型油菜BnaSOT12a基因是与拟南芥中AtSOT12基因同源的一个磺酸转移酶基因。为探寻BnaSOT12a基因的抗性相关功能,对其在油菜中不同部位的表达和在拟南芥中过表达情况下对转基因植株的影响进行了分析。结果显示: BnaSOT12a基因在甘蓝型油菜的根、叶、花芽和花中都有表达,其在花中的表达量最高,而在茎与果荚中没有表达;甘蓝型油菜BnaSOT12a基因在拟南芥中的过表达,能提高转基因植株在发芽和根生长方面对NaCl胁迫的耐受能力,但在盐胁迫下,BnaSOT12a的磺酸化作用并不影响拟南芥中抗病途径的信号传导,与抗病途径无关。
语种：
中文
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水稻多胺转运蛋白OsPUT1基因RNAi载体的构建及遗传转化
作者：
张斌;刘询;耿雅楠;阮颖;黄勇
期刊：
分子植物育种 ,2016年14(10):2653-2658 ISSN：1672-416X
作者机构：
湖南农业大学,生物科学技术学院, 湖南省作物种植资源创新和利用重点实验室, 长沙, 410128;湖南农业大学农学院, 长沙, 410128;[张斌; 刘询; 阮颖; 黄勇] 湖南农业大学,生物科学技术学院, 湖南省作物种植资源创新和利用重点实验室, 长沙, 410128;[耿雅楠] 湖南农业大学农学院, 长沙, 410128
关键词：
遗传转化;日本晴;基因沉默
摘要：
在植物中,多胺(腐胺,亚精胺和精胺)参与许多生理过程,包括胚胎发育、器官形成、花芽发育,叶片衰老,花粉管生长、果实的发育和成熟,生物和非生物胁迫的响应。但是,PAS具有内在毒性和多重功能,细胞内的PA含量受到严格控制,受转运体的调节。因此,在水稻中研究水稻多胺转运蛋白OsPUT1的功能至关重要。本研究以水稻多胺转运蛋白基因OsPUT1为研究对象,通过构建OsPUT1-RNAi载体,借助根癌农杆菌介导水稻遗传转化,将OsPUT1的RNAi表达框转到水稻基因组中;经过PPT抗性筛选与PCR鉴定,成功获得了34株转基因水稻。对其中的7株转基因苗进一步作RT-PCR检测,显示有5株OsPUT1基因表达下调,实现了设计目标。这些转基因水稻为进一步研究OsPUT1的功能与作用机制提供了良好的遗传材料。
语种：
中文
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Evolution and conservation of JmjC domain proteins in the green lineage
作者：
Huang, Yong* ;Chen, Donghong;Liu, Chunlin;Shen, Wenhui;Ruan, Ying*
期刊：
Molecular Genetics and Genomics ,2016年291(1):33-49 ISSN：1617-4615
通讯作者：
Huang, Yong;Ruan, Ying
作者机构：
[Ruan, Ying; Chen, Donghong; Huang, Yong; Shen, Wenhui; Huang, Y; Ruan, Y] Hunan Agr Univ, Int Associated Lab CNRS FU HAU Plant Epigenome Re, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Ruan, Ying; Chen, Donghong; Huang, Yong; Huang, Y; Ruan, Y] Hunan Agr Univ, Dept Hunan Prov Plant Genet & Mol Biol, Key Lab Educ, Changsha 410128, Hunan, Peoples R China.;[Ruan, Ying; Liu, Chunlin] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Shen, Wenhui] Univ Strasbourg, CNRS, Inst Biol Mol Plantes, 12 Rue Gen Zimmer, F-67084 Strasbourg, France.
通讯机构：
[Huang, Y; Ruan, Y] H;Hunan Agr Univ, Int Associated Lab CNRS FU HAU Plant Epigenome Re, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Dept Hunan Prov Plant Genet & Mol Biol, Key Lab Educ, Changsha 410128, Hunan, Peoples R China.
关键词：
JmjC domain;Histone lysine demethylases;Domain organization;Phylogenetic analysis;Evolution
摘要：
Histone modification regulates plant development events by epigenetically silencing or activating gene expression, and histone methylation is regulated by histone lysine methyltransferases (KMTs) and histone lysine demethylases (KDMs). The JmjC domain proteins, an important KDM family, erase methyl marks (CH3-) from histones and play key roles in maintaining homeostasis of histone methylation in vivo. Here, we analyzed 169 JmjC domain proteins from whole genomes of plants ranging from green alga to higher plants together with 36 from two animals (fruit fly and human). The plant JmjC domain proteins were divided into seven groups. Group-I KDM4/JHDM3 and Group-V JMJD6 were found in all the plant species and the other groups were detected mainly in vascular or seed plants. Group-I KDM4/JHDM3 was potentially associated with demethylation of H3K9me2/3, H3K27me2/3, and H3K36me1/2/3, Group-II KDM5A with H3K4me1/2/3, Group-III KDM5B with H3K4me1/2/3 and H3K9me1/2/3, Group-V JMJD6 with H3R2, H4R3, and hydroxylation of H4, and Group-VII KDM3/JHDM2 with H3K9me1/2/3. Group-IV/Group-VI JmjC domain-only A/B proteins were involved in hydroxylation and demethylation of unknown substrate sites. The binding sites for the cofactors Fe(II) and alpha-ketoglutarate in the JmjC domains also were analyzed. In the alpha-ketoglutarate binding sites, Thr/Phe/Ser and Lys were conserved and in the Fe(II) binding sites, two His and Glu/Asp were conserved. The results show that JmjC domain proteins are a conserved family in which domain organization and cofactor binding sites have been modified in some species. Our results provide insights into KDM evolution and lay a foundation for functional characterization of KDMs.
语种：
英文
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罗勒烯信号传导途径各组分突变体高通量活体荧光筛选技术系统的建立
作者：
徐健;李浪;刘琪文;刘春林;阮颖
期刊：
中国农学通报 ,2015年31(14):102-107 ISSN：1000-6850
作者机构：
[徐健; 阮颖] 湖南农业大学生物科学技术院,长沙410128;[徐健; 阮颖] 湖南省植物表观遗传和分子生物学重点实验室,长沙410128;湖南农业大学农学院,长沙410128;[刘琪文] 湖南农业大学生物科学技术院,长沙,410128;[刘春林; 李浪] 湖南省植物表观遗传和分子生物学重点实验室
关键词：
β-罗勒烯;PR1启动子;PDF1.2启动子;荧光素酶基因;活体荧光检测系统
摘要：
为了高效率地发现罗勒烯信号传导途径上的各信号传递成分,本研究拟采用正向遗传学的策略,即利用EMS诱变与荧光素酶-荧光素活体荧光成像检测技术体系相结合的方法筛选突变体.为此,构建了指示基因启动子PR1pro::Luciferase和PDF 1.2pro::Luciferase质粒并转化拟南芥;通过抗性筛选与PCR检测,鉴定获得了T3代转基因纯合子植株.同时,购买了合适的高灵敏CCD相机、暗箱与软件,通过组装调试,成功制造了一台自制的、经济适用的活体荧光检测仪.转基因纯合植株经过茉莉酸、水杨酸或罗勒烯处理和喷施荧光底物后,放到荧光检测仪中,成功观察到了诱导后的转基因植株释放出高亮荧光,说明活体荧光成像检测系统构建成功.这些结果为进一步利用正向遗传学的方法高效筛选罗勒烯信号传导途径中各成分的突变体植株,以及阐明罗勒烯诱导的防御反应的作用机理奠定了良好基础.
语种：
中文
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BolSDG8 RNA干扰载体构建和拟南芥的遗传转化
作者：
常诚;姜玲;阮颖
期刊：
安徽农业科学 ,2015年(16):36-38,84 ISSN：0517-6611
作者机构：
[常诚; 姜玲; 阮颖] 湖南农业大学生物科学技术学院
关键词：
RNA干扰;拟南芥;遗传转化
摘要：
拟南芥SDG8通过调控开花关键基因FLC位点上H3K36的甲基化水平促进其转录表达进而抑制植株早花。甘蓝Bol SDG8是拟南芥SDG8的同源基因,经比对,选择一段约为359 bp的保守序列,命名为Bol SDG8-RNAi,通过构建RNA干扰载体,并将其转化至野生型拟南芥（Col-0）中,获得了稳定遗传的转基因植株。通过对T3代转基因植物的表型观察,开花天数及莲座叶数目的统计分析,发现Bol SDG8 RNA干扰转基因植株表现出与拟南芥sdg8-2突变体相似的生物学表型,即植株弱小,明显早花,暗示Bol SDG8与拟南芥SDG8在调控植物开花上具有相似的生物学功能,为进一步深入研究甘蓝Bol SDG8的生物学功能奠定基础。
语种：
中文
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Characterization of an extracellularly derived α-mannosidase from the liquid exudate of the sclerotia of Sclerotinia sclerotiorum (Lib.) de Bary
作者：
Liu, Zhengli;Wei, Ran;He, Wen;Ruan, Ying;Liu, Chunlin*
期刊：
Antonie van Leeuwenhoek ,2015年108(1):107-115 ISSN：0003-6072
通讯作者：
Liu, Chunlin
作者机构：
[Ruan, Ying; Wei, Ran; Liu, Chunlin; He, Wen; Liu, Zhengli] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Wei, Ran; Liu, Chunlin; He, Wen; Liu, Zhengli] Hunan Agr Univ, Coll Agr, Changsha 410128, Hunan, Peoples R China.;[Ruan, Ying] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Chunlin] H;Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.
关键词：
Sclerotinia sclerotiorum;Sclerotium;Liquid exudate;Non-organelle derived alpha-mannosidase
摘要：
Class I alpha-mannosidases play an important role in co- and post-translational N-glycosylation modification of proteins, and also in glycoprotein glycan hydrolysis. Herein, we investigated a protein named Man-41, from liquid exudate droplets secreted on the surface of developing sclerotia by Sclerotinia sclerotiorum. The protein was identified by MALDI-TOF mass spectrometry to be a alpha-mannosidase. The full-length open reading frame of Man-41 consists of 1581 bp, encoding 526 amino acid residues and containing a putative signal peptide at amino acid residues 1-20, and a conserved sequence at residues 50-521. Man-41 was classified into glycoside hydrolase family 47 (GH47) by clustering analysis. The catalytic residues include Glu(125), Arg(129), Asp(270), Ser(271), Glu(274), Arg(420), Glu(422), Glu(425), Glu(485), Thr(514), and Glu(515), which are conserved in all Class I alpha-1,2-mannosidases. Recombinant Man-41 protein had 26.67 +/- A 2.18 U/mg of alpha-mannosidase activity, about one-half of intracellular mannosidase activity of sclerotia. In conclusion, this is the first time that mannosidase has been identified in an extracellular fluid and Man-41 is also a new member of GH47 with Ca2+-dependent characteristics. This work lays the foundation for further study of the function of Man-41 in sclerotial development.
语种：
英文
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拟南芥AtSb10基因的克隆及其过表达转基因株系的获得
作者：
赵莺婕;刘春林;阮颖
期刊：
浙江农业学报 ,2015年27(9):1550-1554 ISSN：1004-1524
作者机构：
[赵莺婕; 阮颖] 湖南农业大学生物科学技术学院;[刘春林] 湖南农业大学农学院
关键词：
拟南芥;生物信息学分析;表达谱
摘要：
拟南芥At Sb10基因是一个表达谱和功能都未知的基因,生物信息学分析发现其氨基酸序列含有2个连续的MATH结构域。为分析At Sb10基因的功能,首先,利用RT-PCR方法检测At Sb10基因在拟南芥中的表达模式,结果表明,该基因只在根中表达,而茎、叶片、花与果荚中未检测到;然后克隆了At Sb10基因全长,构建了过量表达载体,通过农杆菌介导法将该载体转入拟南芥Col-0中,获得了10株过表达转基因植株,为进一步研究At Sb10基因的功能奠定了基础。
语种：
中文
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不同植物激素诱导拟南芥SDG8的表达模式
作者：
刘玲;金苏;曾文婕;宁凡;阮颖
期刊：
作物研究 ,2015年29(3):226-229 ISSN：1001-5280
作者机构：
湖南农业大学生物科学技术学院,长沙,410128;[刘玲; 金苏; 曾文婕; 宁凡; 阮颖] 湖南农业大学
关键词：
植物激素;拟南芥;表达模式
摘要：
与植物激素在植物整个生命周期中起着多重作用类似,基因SDG8对植物生长、发育、代谢、抗性等多个方面也具多效性。对拟南芥Col-0和转p SDG8：：GUS植株分别进行KT、ABA、GA3、IAA、Me JA和Me SA喷洒处理24h后,用GUS试剂和RT-q PCR研究SDG8基因的表达模式。结果显示,在植物营养生长阶段和使用的激素浓度范围内,这6种激素对SDG8基因的表达调控有4种模式：KT高低浓度都抑制表达;ABA低浓度促进表达,高浓度表达量下降;GA3低浓度与高浓度促进表达的水平相同;随IAA、Me JA和Me SA浓度增加表达水平提高。这些结果从总体上揭示了植物激素对SDG8的表达趋势的影响,对进一步研究植物激素与SDG8基因相互作用有一定的参考价值。
语种：
中文
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黄蜡果种子的解剖结构
作者：
袁波;万丹;何雯;姜玲;阮颖
期刊：
湖南农业科学 ,2014年(1):64-66 ISSN：1006-060X
作者机构：
湖南农业大学生物科学技术学院,湖南 长沙,410128;[何雯; 万丹; 袁波; 阮颖; 姜玲] 湖南农业大学
关键词：
黄蜡果;种子结构;淀粉;油体;蛋白质
摘要：
为了研究黄蜡果种子结构及贮藏物质的组织化学定位，利用细胞学和组织化学等方法观察了黄蜡果种子的解剖结构，并分析了胚、胚乳后含物的类型。结果表明：黄蜡果种子由种皮、胚和胚乳三部分构成，属于双子叶植物有胚乳种子；有发达的胚乳，胚乳细胞的细胞质非常浓厚；成熟胚有两片发育良好且饱满的子叶，子叶比其他双子叶植物种子（如蓖麻）发达；种子含有内外两层种皮，外种皮致密，有一定的光泽度，种皮上覆盖有一定的角质或蜡质；内种皮细胞排列致密与胚乳紧密地相连，且不容易剥离；淀粉主要分布于胚乳和胚根中，蛋白质分布在胚乳、子叶、胚根和胚芽处，油体主要分布在在靠近胚体的胚乳部分（呈U字形），胚和胚乳的其他部分也有分布，但含量相对较低。
语种：
中文
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