Distinct Geographical Distribution of the Miscanthus Accessions with Varied Biomass Enzymatic Saccharification
作者:
Li, Xukai;Liao, Haofeng;Fan, Chunfen;Hu, Huizhen;Li, Ying;...
期刊:
PLOS ONE ,2016年11(8):e0160026 ISSN:1932-6203
通讯作者:
Tu, Yuanyuan
作者机构:
[Liao, Haofeng; Peng, Liangcai; Li, Ying; Hu, Huizhen; Fan, Chunfen; Tu, Yuanyuan; Li, Xukai] Huazhong Agr Univ, Biomass & Bioenergy Res Ctr, Wuhan, Peoples R China.;[Liao, Haofeng; Peng, Liangcai; Li, Ying; Hu, Huizhen; Fan, Chunfen; Tu, Yuanyuan; Li, Xukai] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China.;[Liao, Haofeng; Peng, Liangcai; Li, Ying; Hu, Huizhen; Fan, Chunfen; Li, Jing; Tu, Yuanyuan; Li, Xukai] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Peoples R China.;[Yi, Zili] Hunan Agr Univ, Dept Biotechnol, Changsha, Hunan, Peoples R China.;[Cai, Xiwen] North Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA.
通讯机构:
[Tu, Yuanyuan] H;Huazhong Agr Univ, Biomass & Bioenergy Res Ctr, Wuhan, Peoples R China.;Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China.;Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Peoples R China.
关键词:
Lignin;Cellulose;Plant cell walls;Polymers;Hydrolysis;Hexoses;Insolation;China
摘要:
Miscanthus is a leading bioenergy candidate for biofuels, and it thus becomes essential to characterize the desire natural Miscanthus germplasm accessions with high biomass saccharification. In this study, total 171 natural Miscanthus accessions were geographically mapped using public database. According to the equation [P(H/L| East) = P(H/L∩East)/P(East)], the probability (P) parameters were calculated on relationships between geographical distributions of Miscanthus accessions in the East of China, and related factors with high(H) or low(L) values including biomass saccahrification under 1% NaOH and 1% H2SO4 pretreatments, lignocellulose features and climate conditions. Based on the maximum P value, a golden cutting line was generated from 42°25’ N, 108°22’ E to 22°58’ N, 116°28’ E on the original locations of Miscanthus accessions with high P(H|East) values (0.800–0.813), indicating that more than 90% Miscanthus accessions were originally located in the East with high biomass saccharification. Furthermore, the averaged insolation showed high P (H|East) and P(East|H) values at 0.782 and 0.754, whereas other climate factors had low P(East|H) values, suggesting that the averaged insolation is unique factor on Miscanthus distributions for biomass saccharification. In terms of cell wall compositions and wall polymer features, both hemicelluloses level and cellulose crystallinity (CrI) of Miscanthus accessions exhibited relative high P values, suggesting that they should be the major factors accounting for geographic distributions of Miscanthus accessions with high biomass digestibility.
语种:
英文
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南荻种质农艺及品质性状主成分聚类分析与综合评价
作者:
杨塞;肖亮;王钻;易自力
期刊:
中国草地学报 ,2016年38(3):26-33 ISSN:1673-5021
作者机构:
[杨塞] 湖南农业大学生物科学技术学院,湖南 长沙 410128;湖南农业大学东方科技学院,湖南 长沙 410128;芒属植物生态应用技术湖南省工程实验室,湖南 长沙,410128;湖南农业大学生物科学技术学院,湖南 长沙,410128;[王钻; 易自力] 湖南农业大学
关键词:
南荻;农艺性状;品质性状;主成分分析;聚类分析
摘要:
对30份不同来源南荻种质资源的9个农艺性状(叶茎比、茎壁厚、株高、节数、最大节长、茎粗、单株重、分枝数和分蘖数)和6个品质性状(含水量、灰分、半纤维素、纤维素、木质素和热值)进行综合评价,结果表明:(1)南荻种质资源农艺性状和品质性状的变异系数变化范围为4.15%~75.02%;(2)主成分分析表明,前5个主成分因子解释了88.68%的变异源,第1主成分因子载荷最高的性状是株高、分蘖数、茎粗,说明产量及其构成因子是南荻的主要变异来源;(3)聚类分析表明,30份南荻种质可划分为3大类,第Ⅰ类群由8份来自洞庭湖地区的种质组成,第Ⅱ类群由14份来自鄱阳湖和长江中下游地区的种质组成,第Ⅲ类群由8份来自长江下游地区的种质组成;(4)通过主成分的综合线性模型对30份南荻种质资源进行综合评价,结果显示来自湖南澧县(编号B0634)种质综合评分最高,为2.778。
语种:
中文
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不同南荻繁殖体幼苗生长研究
作者:
魏娟;肖亮;王学华;易自力
期刊:
作物研究 ,2016年30(1):58-62 ISSN:1001-5280
作者机构:
湖南农业大学农学院,长沙,410128;湖南农业大学生物质醇类燃料湖南省工程实验室,长沙,410128;[易自力] 3湖南农业大学生物科学技术学院,长沙,410128;[肖亮; 王学华; 魏娟] 湖南农业大学
关键词:
南荻;繁殖;地下茎;顶芽;切段
摘要:
为给南荻(Miscanthus lutarioriparius)大规模繁殖和生产实践提供参考,以南荻不同地下茎繁殖体(1年生切段、1年生顶芽、3年生切段、3年生顶芽、6年生切段)为材料,通过对出苗率、株高、叶数、倒3叶长等指标的观察,研究了繁殖母体对南荻幼苗生长的影响。结果表明:不同繁殖母体长成的幼苗在出苗率、株高、叶数、倒3叶、地下芽长和地下部干重上都表现为地下茎切段>地下茎顶芽、1年生>3年生>6年生,说明南荻幼苗繁殖以年限较短的地下茎切段作为繁殖材料效果较好。
语种:
中文
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芒属植物AFLP分析体系的建立与优化
作者:
黄红梅;刘清波;易自力;陈智勇;覃静萍
期刊:
分子植物育种 ,2016年14(1):204-209 ISSN:1672-416X
作者机构:
[黄红梅; 刘清波; 易自力; 陈智勇; 覃静萍] 湖南农业大学生物科学技术学院, 长沙, 410128
关键词:
芒属;扩增片段长度多态性;分子标记;体系
摘要:
对AFLP实验流程中的基因组提取、酶切等关键因素进行优化,建立了芒属植物的AFLP (扩增片段长度多态性)分析体系。本研究分别采用常规CTAB法和改良的CTAB法提取芒属植物基因组DNA,基因组分别用HindⅢ/Mse I系统和Pst I /Mse I系统进行酶切和连接一步反应,连接产物稀释20倍后进行预扩增,预扩产物稀释20倍后进行选择性扩增。结果显示:采用改良CTAB法提取的DNA质量优于常规CTAB法;采用Pst I /Mse I系统酶切的DNA多态性高于HindⅢ/Mse I系统。采用优化后的AFLP体系分析30份供试芒属植物材料,从64对引物中筛选出8对多态性较好的AFLP选择性扩增引物,多态性比率达到100%,表明优化后的AFLP体系适合于芒属植物的分子标记分析,为芒属植物种质遗传多样性的分子研究奠定了技术基础。
语种:
中文
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Improvement of bacterial blight and brown planthopper resistance in an elite restorer line Huazhan of Oryza
作者:
Xiao, Youlun;Li, Jinjiang;Yu, Jianghui;Meng, Qiucheng;Deng, Xiangyang;...
期刊:
Field Crops Research ,2016年186:47-57 ISSN:0378-4290
通讯作者:
Xiao, Guoying
作者机构:
[Xiao, Youlun; Yi, Zili] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Xiao, Youlun; Xiao, Guoying; Li, Jinjiang; Meng, Qiucheng; Yu, Jianghui; Deng, Xiangyang] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.;[Xiao, Youlun] Hunan Acad Agr Sci, Hunan Plant Protect Inst, Changsha 410125, Hunan, Peoples R China.
通讯机构:
[Xiao, Guoying] C;Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.
关键词:
Bacterial blight resistance;Brown planthopper resistance;Gene pyramiding;Hybrid rice;Restorer line
摘要:
Development of resistant varieties is one of the most economical and effective strategies to prevent rice from bacterial blight disease (BB) and brown planthopper (BPH), the two main pests jeopardizing rice production. The hybrid rice Tianyouhuazhan (TianfengA/Huazhan) has been widely used in rice production in China, but this hybrid is susceptible to BB and BPH. In this study, one BB resistance gene (Xa23) and two BPH resistance genes (Bph14 and Bph15) were simultaneously introgressed into the restorer line Huazhan to improve the BB and BPH resistance of Tianyouhuazhan using marker-assisted backcrossing (MABC) strategy coupled with phenotypic selection. The results of identification of BB and BPH resistance revealed that almost all of the improved restorer lines and their derived hybrids showed high resistance (HR) or resistance (R) to BB and BPH. Almost all of the agronomic traits of improved restorer lines and their hybrids were similar to those of their respective original versions in field trial, except the 1000-grain weight of improved hybrids showed an extremely significant increase than that of Tianyouhuazhan. Plot yields test in 2013 and 2014 suggested that two improved hybrid combinations named as TianfengA/R43-06 and TianfengA/R43-07 had higher grain yields per plot than Tianyouhuazhan and other improved hybrids. These results clearly indicate that pyramiding of Xa23, Bph14 and Bph15 genes is a useful approach for improving BB and BPH resistance, two hybrid combinations TianfengA/R43-06 and TianfengA/R43-07 could replace Tianyouhuazhan for extension in BB and/or BPH epidemic area in China. © 2015 Elsevier B.V.
语种:
英文
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芒属植物能源潜力评价体系的构建
作者:
项伟;易自力;肖亮;刘清波;覃静萍
期刊:
中国农业科学 ,2016年49(24):4687-4700 ISSN:0578-1752
作者机构:
湖南农业大学生物科学技术学院,长沙,410128;[易自力] 湖南农业大学生物科学技术学院,长沙410128;[易自力] 芒属植物生态应用技术湖南省工程实验室,长沙410128;芒属植物生态应用技术湖南省工程实验室,长沙,410128;[覃静萍; 项伟; 刘清波] 湖南农业大学
关键词:
芒属;能源植物;层次分析法;评价体系
摘要:
【目的】芒属植物是目前国内外生物质能源领域的研究热点,在全球共有14个种,且具有种间杂交的特点。文章为建立芒属植物能源潜力评价体系,为从众多的芒属基因型中筛选优良种质和选育能源作物品种奠定基础。【方法】通过文献法、调研法和专家咨询法探讨了芒属植物作能源作物应用的相关性状指标。运用层次分析法,根据能源用途不同按发电、制乙醇、产沼气、制生物油等4类,分别从农艺、品质、抗逆性等3方面,选取干物质产量、冠层高、茎高、茎径、基部直径、分蘖数、叶茎比、枯黄性、落叶性、含水量、灰分含量、挥发分含量、固定碳含量、热值、纤维素含量、半纤维素含量、木质素含量、矿质元素含量、耐盐性、抗旱性、抗寒性、耐淹性、抗病虫性等23项指标来构建芒属能源植物评价指标体系,同时制定了性状测量标准和能源利用潜力指数计算法则。然后使用该体系对湖南农业大学芒属植物资源圃中B0340(芒)、 A0504(五节芒)、A0123(荻)、A0118(南荻)、D0302(奇岗)5个典型的芒属种质代表进行了测量和评价示范。再将评价结果与现有文献中有关发电、制乙醇和产沼气的能源理论产率计算公式的计算结果予以比对。【结果】芒属植物能源利用潜力指数分成4级,分别为极宜(75—100分)、适宜(50—74分)、一般(25—49分)、不宜(0—24分)。示范评价结果显示:A0504(五节芒)发电得60.73分,制乙醇得60.14分,产沼气得60.27分,制生物油得57.19分;A0118(南荻)发电得64.32分,制乙醇得58.45分,产沼气得58.20分,制生物油得60.01分;D0302(奇岗)发电得54.06分,制生物油得50.33分。可知A0504、A0118、D0302为适宜的能源植物,发酵制乙醇和沼气最适用A0504为原料,燃烧发电和热裂解制油最适用A0118为原料。此结果与现有文献中有关发电、制乙醇和产沼气的能源理论产率计算公式的计算结果基本一致,且比能源理论产率计算公式更能真实反映实际生产情况。【结论】通过从农艺、品质、抗逆性等3方面构建的芒属植物能源潜力评价体系,能够在发电、制乙醇、产沼气和制生物油4类能源用途方面客观评价芒属植物的适用性,从而可用于其优良种质的筛选和新品种的选育。
语种:
中文
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A transcriptome-wide study on the microRNA- and the Argonaute 1-enriched small RNA-mediated regulatory networks involved in plant leaf senescence
作者:
Qin, J.;Ma, X.;Yi, Z.;Tang, Z.* ;Meng, Y.*
期刊:
Plant Biology ,2016年18(2):197-205 ISSN:1435-8603
通讯作者:
Tang, Z.;Meng, Y.
作者机构:
[Yi, Z.; Qin, J.; Tang, Z.] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Ma, X.; Meng, Y.] Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou, Zhejiang, Peoples R China.;[Meng, Y.] Xuelin St 16, Hangzhou 310036, Zhejiang, Peoples R China.
通讯机构:
[Tang, Z.] H;[Meng, Y.] X;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Xuelin St 16, Hangzhou 310036, Zhejiang, Peoples R China.
关键词:
Argonaute 1;leaf senescence;microRNA;protein interaction;senescence associated gene;small RNA
摘要:
Leaf senescence is an important physiological process during the plant life cycle. However, systemic studies on the impact of microRNAs (miRNAs) on the expression of senescence-associated genes (SAGs) are lacking. Besides, whether the other Argonaute 1 (AGO1)-enriched sRNAs play regulatory roles in leaf senescence remains unclear. In this study, a total of 5,123 and 1,399 AGO1-enriched sRNAs, excluding miRNAs, were identified in Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa), respectively. After retrieving the SAGs from Leaf Senescence Database, all of the AGO1-enriched sRNAs and the miRBase-registered miRNAs of the two plants were included for target identification. Supported by degradome signatures, 200 regulatory pairs involving 120 AGO1-enriched sRNAs and 40 SAGs, and 266 regulatory pairs involving 64 miRNAs and 42 SAGs were discovered in Arabidopsis. Moreover, 13 genes predicted to interact with some of the above identified target genes at protein level were validated to be regulated by 17 AGO1-enriched sRNAs and 10 miRNAs in Arabidopsis. In rice, only one SAG was targeted by three AGO1-enriched sRNAs, and one SAG was targeted by miR395. However, five AGO1-enriched sRNAs were conserved between Arabidopsis and rice. Target genes conserved between the two plants were identified for three of the above five sRNAs, pointing to the conserved roles of these regulatory pairs in leaf senescence or other developmental procedures. Novel targets were discovered for three of the five AGO1-enriched sRNAs in rice, indicating species-specific functions of these sRNA-target pairs. These results could advance our understanding of the sRNA-involved molecular processes modulating leaf senescence. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
语种:
英文
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A comparative study of two-line early season hybrid rice with lepidopteran resistance
作者:
Liu, Wenqiang;Meng, Qiucheng;Weng, Lushui;Peng, Jian;Xiao, Youlun;...
期刊:
Field Crops Research ,2016年187:107-112 ISSN:0378-4290
通讯作者:
Xiao, Guoying
作者机构:
[Yi, Zili; Liu, Wenqiang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Xiao, Guoying; Meng, Qiucheng; Weng, Lushui; Yu, Jianghui] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.;[Liu, Wenqiang] Hunan Acad Agr Sci, Hunan Rice Res Inst, Changsha 410125, Hunan, Peoples R China.;[Peng, Jian] Changde Res Inst Agr Sci, Changde 415000, Peoples R China.;[Xiao, Youlun] Hunan Acad Agr Sci, Hunan Plant Protect Inst, Changsha 410125, Hunan, Peoples R China.
通讯机构:
[Xiao, Guoying] C;Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.
关键词:
Cry2Aa#;Hybrid;Oryza stativa;Rice leaf roller
摘要:
In our previous study, an early season rice restorer line B2A68 expressing Cry2Aa# gene was obtained. In order to evaluate the performance of B2A68's hybrids, a total of six hybrids from crossing B2A68 with six thermo-sensitive genic male sterile (TGMS) lines of early season rice were compared for expression level of Cry2Aa protein, as well as in insect resistance, agronomic traits and grain quality with their non-Bt null controls. Our results showed that the highest Cry2Aa protein content among B2A68's hybrids was detected in leaf, where the Cry2Aa content was nearly three times of that in stem. The Cry2Aa content of B2A68 was significantly higher than those of its derived hybrids, indicating the Cry2Aa# gene was incompletely dominant in hybrids. The hybrids with Cry2Aa# gene exhibited higher plant protection against rice leaf roller than the hybrids without Cry2Aa# gene in field. Significant differences were observed in plant height, panicle length, number of grains per panicle, seed set, 1000-grain weight and yield per plant between hybrids with Cry2Aa# gene and hybrids without Cry2Aa# gene in 2013 or 2014. There were significant differences in chalky grain rate and chalkiness degree between paternal lines B2A68 and D68, and between hybrids with Cry2Aa# gene and hybrids without Cry2Aa# gene. All of the differences in agronomic traits and grain quality varied among parental lines and hybrids. In this paper, three hybrids with high lepidopteran resistance, high yield, elite agronomic traits and acceptable grain quality was selected out, that could serve as elite resistant hybrids to control lepidopteran pests in early season rice production. © 2015 Elsevier B.V.
语种:
英文
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Assessment of the production potentials of Miscanthus on marginal land in China
作者:
Xue, Shuai;Lewandowski, Iris;Wang, Xiaoyu;Yi, Zili*
期刊:
Renewable & Sustainable Energy Reviews ,2016年54:932-943 ISSN:1364-0321
通讯作者:
Yi, Zili
作者机构:
[Xue, Shuai; Lewandowski, Iris] Univ Hohenheim, Inst Crop Sci, Dept Biobased Prod & Energy Crops 340b, D-70599 Stuttgart, Germany.;[Xue, Shuai; Yi, Zili] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Wang, Xiaoyu] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Yi, Zili] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词:
Bioenergy potential;CO2 mitigation;Marginal land;Miscanthus;Yield model
摘要:
Miscanthus is characterized by high biomass production potential and low input requirements and therefore considered a leading candidate for second-generation energy crops. Because China has limited agricultural land resources, development of its miscanthus-based bioenergy industry must rely on the use of marginal land. This study focuses on the assessment of the production potential of miscanthus on China's marginal land, which in this context is defined as land presently not used for agricultural production, residential purposes and other social uses. Geographic Information System (GIS) techniques and model simulation are adopted to identify the productive marginal areas for miscanthus and to estimate their biomass and bioenergy production potential. The results show that although a large marginal area of 17,163.54×104 ha is available for producing miscanthus, due to the limitation of low winter temperatures and low precipitation levels in some areas, the total suitable marginal area is only 769.37×104 ha. The Monteith radiation yield model was used to determine the potential miscanthus yield in Chinese climatic conditions. The simulation gave actual harvestable yield levels on arable land of 18.1-44.2 t ha-1 yr-1. Taking the environmental stresses of marginal conditions into account then gave an achievable miscanthus yield potential on marginal land of 2.1-32.4 t ha-1 yr-1 (average for the different marginal lands). Based on these achievable yield levels, the total biomass production potential on the entire marginal area is 13,521.7×104 t yr-1; the bio-electricity generation and total greenhouse gas saving potential from these biomasses are 183.9 TW h yr-1 and 21,242.4×104 t CO2 eq. yr-1, respectively. The spatial distribution of the suitable marginal areas shows that they are mainly concentrated in the central part of Northeast China and the Loess Plateau. Both regions are recommended as priority development zones for the Chinese miscanthus-based bioenergy industry. © 2015 Elsevier Ltd. All rights reserved.
语种:
英文
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稻壳炭和腐殖酸对水稻抗逆生理与产量的影响
作者:
邢嘉韵;易自力;肖亮;范星露;郭亮;...
期刊:
作物研究 ,2016年30(4):387-392 ISSN:1001-5280
作者机构:
湖南农业大学生物科学技术学院,长沙,410128;湖南农业大学教育学院,长沙,410128;[肖亮; 郭亮; 王惠群; 易自力; 范星露; 邢嘉韵] 湖南农业大学
关键词:
水稻;稻壳炭;腐殖酸;抗逆性;产量
摘要:
以晚稻‘ 株两优819 ’ 为材料,采用随机区组试验设计,在田间研究稻壳炭和腐殖酸不同施用量对水稻产量的影响;以早稻‘ 湘早籼4 5 号’ 为材料, 采用盆栽试验,研究稻壳炭与腐殖酸对水稻抗逆生理和光合作用的影响.结果表明:随着稻壳炭、腐殖酸施用量的增加,水稻产量都增加;稻壳炭和腐殖酸都能显著提高水稻叶片的光合能力和抗氧化酶活性.因此,在大田施用稻壳炭和腐殖酸能够提高水稻的产量.
语种:
中文
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4种芒属植物光合特性研究
作者:
覃静萍;易自力;肖亮;黄红梅
期刊:
草地学报 ,2015年23(4):752-757 ISSN:1007-0435
作者机构:
湖南农业大学生物科学技术学院;[肖亮] 生物质醇类燃料湖南省工程实验室;[覃静萍; 易自力; 黄红梅] 湖南农业大学
关键词:
芒属植物;光响应;净光合速率;水分利用率
摘要:
以芒(Miscanthus sinensis)、五节芒(M. floridulus) 、荻(M. sacchariflorus)和南荻(M. lutarioriparius)等4种典型芒属植物为试验材料,用LI-6400便携式光合仪测量不同时期的光合特性,来研究芒属植物光响应及光合特性的季节性变化规律。结果表明:光合作用参数对不同光合有效辐射的响应在种间有差异,除南荻的光饱和点(LSP)在800 μmol· m~(-2) ·s~(-1)左右外,其他3种约在1000 μmol ? m~(-2) ? s~(-1) ;最大净光合速率(P_(nmax))由大到小依次为荻(47. 3 μmol . m~(-2) . s~(-1))、南荻(38. 6 μmol . m~(-2) ? s-1)、五节芒(20. 4 μmol ? m~(-2) ? s~(-1))、芒(8. 0 μmol ? m~(-2) ? s-1);表观量子效率(AQY)依次为荻(0. 123)、南荻(0. 108)、五节芒(0. 089)、芒(0. 022),荻和南荻的光能转化率强于五节芒和芒。主生长季的净光合速率(P_n)表现出明显的季节性变化,并与生长速率同步,种间差异极显著(P<0. 01),荻和南荻的P_n值高于芒和五节芒。4种芒属植物在主生长季的水分利用率(WUE)最小值在6-7月,最大值在8-9月,表现出耐旱耐高温能力,且种间差异显著(P<0. 05)。
语种:
中文
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奇雅1号在湖南地区的种植表现及产量相关性分析
作者:
胡忠红;易自力;李娇娇;刘希萌;陈智勇
期刊:
南方农业 ,2015年9(19):13-15 ISSN:1673-890X
作者机构:
[胡忠红; 李娇娇; 刘希萌] 湖南农业大学东方科技学院;湖南农业大学生物科学技术学院;[陈智勇; 易自力] 湖南农业大学
关键词:
奇雅1号;非主要农作物;新品种;种植表现;产量相关性分析;湖南省
摘要:
首次报道非主要农作物新品种''奇雅1号''在湖南省5个不同试种点的种植表现及主要栽培管理技术,并对其农艺性状进行连续2年的观测记录,重点分析与产量相关的性状.结果表明:奇雅1号适应亚热带季风性湿润气候,生长期在200 d左右,是典型的短日照植物;播种时间为4月下旬或5月上旬;发芽的最适气温为25℃;播种基质为pH 6.5~7.0的沙壤或粘土;收获时间为11月下旬;通过主成分分析,在众多的农艺性状中,与产量高度相关的是分枝数和花序数.
语种:
中文
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抗褐化剂对荻外植体褐化和愈伤组织生长的影响
作者:
彭思佳;丁力;刘清波;陈智勇;肖亮;...
期刊:
草原与草坪 ,2015年35(5):7-11,16 ISSN:1009-5500
作者机构:
[陈智勇; 肖亮; 丁力; 易自力; 彭思佳; 刘清波] 湖南农业大学 生物科学技术学院芒属植物研究所,湖南 长沙,410128;[蒋建雄] 湖南农业大学 生物科学技术学院芒属植物研究所,湖南 长沙 410128;江苏大学 生物质能源研究所,江苏 镇江 212013;[蒋建雄] 江苏大学 生物质能源研究所,江苏 镇江 212013
关键词:
荻;抗褐化剂;褐化;愈伤生长
摘要:
以荻(Miscanthus sacchariflorus)幼穗和诱导出的愈伤组织为材料探讨抗褐化剂聚乙烯吡咯烷酮(PVP)、抗坏血酸(Vc)、柠檬酸(CA)对外植体和愈伤组织抗褐化作用,以及愈伤诱导率及愈伤生长速度的影响。结果表明,3种抗褐化剂都能抑制外植体和愈伤组织褐化,并提高愈伤组织诱导率。添加0.2g/L PVP时荻外植体的褐变程度最低,0.2g/L CA时愈伤组织诱导率最高,为91.1%,向愈伤组织继代培养基中添加0.2g/L的Vc愈伤组织重量增加倍数最高,为21.38倍,且愈伤组织褐化率低为25.8%。
语种:
中文
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南荻衰老的形态和生理生化特征的研究
作者:
李莎莎;艾辛;龙卫;易自力;黄红梅;...
期刊:
草地学报 ,2015年23(1):215-218 ISSN:1007-0435
作者机构:
湖南农业大学生物科学技术学院,湖南长沙,410128;[艾辛] 湖南农业大学生物科学技术学院,湖南长沙410128;[艾辛] 湖南农业大学园艺园林学院,湖南长沙410128;[李莎莎; 肖亮; 易自力; 黄红梅; 龙卫] 湖南农业大学
关键词:
南荻;形态特征;生理生化;叶片衰老
摘要:
为探讨南荻(Miscanthus lutariori parius)衰老的形态与生理生化特征间的关系,以5种基因型的南荻为试材料,利用目测分级法对其衰老程度进行分级,并测定了旗叶、倒二叶和倒三叶生理生化指标。结果表明:南荻叶片的衰老等级与叶绿素含量成显著负相关,丙二醛(MDA)含量从孕穗期至果实成熟期逐渐增加,超氧化物歧化酶(SOD)活性从孕穗期到抽穗期处于上升阶段,抽穗后到成熟期呈下降趋势;南荻衰老程度在空间上并无规律,但衰老速度在不同基因型间存在差异。
语种:
中文
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芒种内F_1杂交种的配制及杂种真实性的分子鉴定
作者:
丁力;申方群;黄红梅;肖亮;易自力;...
期刊:
中国草地学报 ,2015年37(2):53-57,80 ISSN:1673-5021
作者机构:
湖南农业大学生物科学技术学院,湖南 长沙,410128;湖南农业大学东方科技学院,湖南 长沙,410128;[蒋建雄] 湖南农业大学生物科学技术学院,湖南 长沙410128;[蒋建雄] 江苏大学环境与安全工程学院生物质能源研究所,江苏镇江212013;[肖亮; 丁力; 易自力; 黄红梅] 湖南农业大学
关键词:
芒;种内杂交;杂种鉴定
摘要:
通过人工杂交方法,建立了一个包含有142个单株的芒正反交F_1后代群体,并采用SSR分子标记对杂交种进行鉴定,结果表明:以HAU116和HAU388两个SSR标记表现为共显性,鉴定出的真杂种个体数分别是121株和138株;而以HAU72、HAU384和HAU517三个标记表现为显性,其中HAU72标记从C0344(♀)×A0420(♂)后代群体中鉴定出54株为真杂种,HAU384和HAU517标记则从A0420(♀)×C0344(♂)后代群体中分别鉴定出48株和47株为真杂种。尽管不同标记的鉴定结果不完全一致,但后代群体中的每一个体均被至少一个标记鉴定为真实杂种。
语种:
中文
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Intronic regions of plant genes potentially encode RDR (RNA-dependent RNA polymerase)-dependent small RNAs
作者:
Qin, Jingping;Ma, Xiaoxia;Yi, Zili;Meng, Yijun;Tang, Zhonghai*
期刊:
JOURNAL OF EXPERIMENTAL BOTANY ,2015年66(7):1763-1768 ISSN:0022-0957
通讯作者:
Tang, Zhonghai
作者机构:
[Yi, Zili; Qin, Jingping; Tang, Zhonghai] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Meng, Yijun; Ma, Xiaoxia] Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 310036, Zhejiang, Peoples R China.
通讯机构:
[Tang, Zhonghai] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词:
Arabidopsis thaliana;double-stranded RNA precursor;intron;phase-distributed;RDR (RNA-dependent RNA polymerase);small RNA
摘要:
Recent research has linked the non-coding intronic regions of plant genes to the production of small RNAs (sRNAs). Certain introns, called 'mirtrons' and 'sirtrons', could serve as the single-stranded RNA precursors for the generation of microRNA and small interfering RNA, respectively. However, whether the intronic regions could serve as the template for double-stranded RNA synthesis and then for sRNA biogenesis through an RDR (RNA-dependent RNA polymerase)-dependent pathway remains unclear. In this study, a genome-wide search was made for the RDR-dependent sRNA loci within the intronic regions of the Arabidopsis genes. Hundreds of intronic regions encoding three or more RDR-dependent sRNAs were found to be covered by dsRNA-seq (double-stranded RNA sequencing) reads, indicating that the intron-derived sRNAs were indeed generated from long double-stranded RNA precursors. More interestingly, phase-distributed sRNAs were discovered on some of the dsRNA-seq read-covered intronic regions, and those sRNAs were largely 24 nt in length. Based on these results, the opinion is put forward that the intronic regions might serve as the genomic origins for the RDR-dependent sRNAs. This opinion might add a novel layer to the current biogenesis model of the intron-derived sRNAs. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.
语种:
英文
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不同基因型南荻愈伤组织诱导及分化的差异
作者:
彭思佳;肖亮;刘清波;易自力;蒋建雄
期刊:
湖南农业科学 ,2015年(9):8-11 ISSN:1006-060X
作者机构:
[肖亮; 易自力; 彭思佳; 刘清波] 湖南农业大学生物科学技术学院芒属植物研究所,湖南 长沙,410128;[蒋建雄] 湖南农业大学生物科学技术学院芒属植物研究所,湖南 长沙 410128;[蒋建雄] 江苏大学生物质能源研究所,江苏 镇江 212013
关键词:
南荻;愈伤组织;诱导率;分化率;茎秆成份
摘要:
以8种基因型南荻(Miscanthus lutarioriparius)幼穗为材料进行组织培养,并对其茎秆成份含量进行测定.结果表明:不同基因型南荻愈伤组织诱导率和分化率均存在显著差异,A4和A6均最高:诱导率分别为97.5%和94.2%、分化率分别为91.8%和83.3%.对南荻诱导率和分化率与茎秆成份进行回归分析显示:愈伤组织诱导率和分化率均与茎秆中半纤维素、木质素含量、105℃含水量存在显著相关性,此外,分化率还与45℃总含水量有显著相关性.
语种:
中文
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低温和植物生长物质对南荻种子萌发的影响
作者:
魏娟;肖亮;易自力;王学华;杨塞;...
期刊:
湖南农业大学学报(自然科学版) ,2015年41(6):616-620 ISSN:1007-1032
作者机构:
湖南农业大学农学院;[陈智勇; 肖亮; 王学华; 魏娟; 易自力; 杨塞] 湖南农业大学;湖南农业大学生物科学技术学院
关键词:
南荻;低温;赤霉素;吲哚乙酸;6–苄氨基腺嘌呤;2,4–二氯苯氧乙酸;种子萌发
摘要:
以南荻种子为材料,设4℃低温处理(常温为CK)和GA3、IAA、6–BA和2,4–D 4种植物生长物质(各设4个质量浓度50、100、200、400 mg/L),均以清水为CK,处理南荻种子,测定南荻种子的发芽率、发芽势、发芽指数、活力指数、根长以及芽长等指标。结果表明,与CK相比,4℃低温处理能显著提高南荻种子的发芽率、发芽势,极显著提高南荻种子的发芽指数、活力指数、根长,但芽长差异没有统计学意义;各质量浓度的GA3能显著或极显著提高发芽率、发芽势、发芽指数、活力指数和芽长,其中100 mg/L GA3处理对南荻种子萌发的效果较好;除400 mg/L外,IAA各质量浓度均能提高发芽率、发芽势、活力指数和促进根的生长,其中以50 mg/L IAA处理对南荻种子的萌发效果较好;各质量浓度的6–BA处理能极显著提高南荻种子的发芽率、发芽指数,显著或极显著提高南荻种子的发芽势和促进芽的生长,极显著的降低南荻种子的活力指数和抑制根的生长;2,4–D对南荻种子发芽率、发芽势以及发芽指数的影响都随浓度的增加先升高后下降,各质量浓度的2,4–D可极显著降低南荻种子的活力指数和抑制根的生长,其中以200 mg/L 2,4–D处理对南荻种子萌发的效果较好。
语种:
中文
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野生南荻与芒杂种多倍体诱导研究
作者:
黄丽芳;殷绪明;杨宇晨;李伟丽;易自力
期刊:
西北植物学报 ,2015年35(1):50-56 ISSN:1000-4025
作者机构:
[黄丽芳; 殷绪明; 李伟丽] 中国科学院亚热带农业生态研究所;[杨宇晨; 易自力] 湖南农业大学物科学技术学院
关键词:
芒属植物;杂交后代;染色体加倍;秋水仙碱;异源四倍体
摘要:
用不同浓度秋水仙素处理野生南荻× 芒(Miscanthus lutarioriparia × Miscanthus sinensis)远缘杂交后代以诱导产生多倍体,并对变异株进行形态学和细胞学鉴定,以期获得稳定的四倍体植株并分析其生理特性。结果表明:(1)采用秋水仙素加入培养基处理法和秋水仙素溶液浸泡处理法都可获得一定频率的多倍体植株:胚性愈伤组织以0.2%秋水仙素浸泡处理48 h的诱变效果较好,四倍体诱导率达8.7%;芽在0.05%秋水仙素培养基中处理15 d较好,四倍体诱导率达10. 6%;生根苗在0.1%秋水仙素培养基中处理10 d较好,四倍体诱导率达11. 1%。(2)经体细胞染色体计数,加倍植株染色体数为2n = 4x=76,对照植株的染色体数目为2n = 2x=38。 (3)生长2年的多倍体植株形态、叶片大小、茎粗、茎壁厚、节间等性状表现出巨大性和超亲优势。
语种:
中文
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芒属能源植物种子适宜采收时期研究
作者:
易镇邪;王禹;王学华;屠乃美;易自力
期刊:
华北农学报 ,2015年(S1):231-234 ISSN:1000-7091
作者机构:
湖南农业大学 农学院,湖南 长沙,410128;湖南农业大学 生物科学技术学院,湖南 长沙,410128;[王禹; 屠乃美; 易镇邪; 王学华; 易自力] 湖南农业大学
关键词:
芒属能源植物;种子;发芽特性;采收时间
摘要:
为确定芒属植物种子的适宜采收时期,研究了采收时间对3个芒属植物野生材料与3个芒属植物人工杂交种种子发芽特性的影响,结果表明,采收时间对芒属植物种子发芽率、发芽势、发芽指数、根芽比等影响显著,根据以上指标确定了各材料种子的适宜采收时间,A0104、湘杂芒3号为11月20日,A0107、B0605、湘杂芒1号为12月20日,而湘杂芒2号为1月20日。
语种:
中文
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