摘要：
Abstract The relationship between nitrogen efficiency (NE), defined as seed yield per unit nitrogen (N) application, and seed quality was examined in two oilseed rape (Brassica napus L.) varieties at 5 N application levels, 0.6, 3, 6, 12, 15 mmol L−1, N1, N2, N3, N4 and N5, respectively. Seed yield, oil yield and protein content were increased with the increase in N application level, but NE and oil content were decreased, and the fatty acid composition in seed was hardly changed. Analysis of seven fatty acids revealed aslight decrease in the contents of erucic acid and arachidonic acid with the increase in N application level, but no obvious change in the contents of palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid. Compared with the low NE variety H29, the seed yield and contents of erucic acid and arachidonic acid in the high NE variety bin270 were more markedly increased with the increase in N application level, and the oil content was hardly changed. The seed yield, oilcontent and oil yield were higher in the high NE variety than in the low NE variety at all 5 N application levels. There were no significant differences in protein, palmitic acid, stearic acid and oleic acid contents between the varieties at any of the 5 N application levels, but there were slight differences in the linoleic acid and linolenic acid contents between the two varieties. In brief, N application improved oil yield more greatly in the high NE variety than in the low NE variety, but hardly affected the fatty acid composition. Therefore, the seed quality and oil content of oilseed rape may not be decreased by breeding of a high NE variety with a high N absorption efficiency and high N use efficiency.
作者机构：
[Zheng Xiaodong; Zhang Zhenhua; Rong Xiangmin; Luo Zunchang; Liu Qiang; Song Haixing; Peng Jianwei; Xie Guixian; Zhang Yuping; Gu Jidong] College of Resources and Environment,Hunan Agricultural University;[Zheng Xiaodong; Zhang Zhenhua; Rong Xiangmin; Luo Zunchang; Liu Qiang; Song Haixing; Peng Jianwei; Xie Guixian; Zhang Yuping; Gu Jidong] Soil and Fertilizer Institute of Hunan Province;[Zheng Xiaodong; Zhang Zhenhua; Rong Xiangmin; Luo Zunchang; Liu Qiang; Song Haixing; Peng Jianwei; Xie Guixian; Zhang Yuping; Gu Jidong] Hunan Provincial Key Lab. Plant Nutrition Common University;[Zheng Xiaodong; Zhang Zhenhua; Rong Xiangmin; Luo Zunchang; Liu Qiang; Song Haixing; Peng Jianwei; Xie Guixian; Zhang Yuping; Gu Jidong] Hunan Provincial Key Lab. Farmland Pollution Control Agri. Resources Use;[Zheng Xiaodong; Zhang Zhenhua; Rong Xiangmin; Luo Zunchang; Liu Qiang; Song Haixing; Peng Jianwei; Xie Guixian; Zhang Yuping; Gu Jidong] School of Biological Sciences,The University of Hong Kong
会议名称：
Environmental Pollution and Public Health(EPPH 2012)
会议时间：
2012-05-17
会议地点：
中国上海
摘要：
In order to study the research development of different remediation methods and the future works of heavy metal pollution, we will give a brief summary of current situation of soil heavy metal polluti
期刊：
Australian Journal of Crop Science,2012年6(9):1377-1382 ISSN：1835-2693
通讯作者：
Song, H.-X.
作者机构：
College of Agricultural Resources and Environment, Hunan Agricultural University, Changsha, China;Hunan Provincial Key Laboratory of Plant Nutrition in Common University, Changsha, 410128, China;Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use, Changsha, 410128, China;National Engineering Laboratory of High Utilization Efficiency of Soil Fertilizer Resource, Changsha, 410128, China;National Center of Oilseed Crops Improvement Hunan Branch, Changsha, 410128, China
通讯机构：
College of Agricultural Resources and Environment, Hunan Agricultural University, China
关键词：
Nitrate nitrogen;Nitrogen use efficiency;Proton pump of tonoplast
摘要：
Large amount of N03 --N are accumulated in vacuole, and cannot be timeously reducted, reutilized and transported into cytoplasm. It is the main reason for great N03--N accumulation in vacuole and nitrogen (N) use efficiency cannot be further improved. Transport mechanism of N03--N across tonoplast is explained in this paper, there are two proton pumps (H+-ATPase and H+-PPase) on tonoplast with absolutely different biology functions and physical characteristic. Mg·ATP and Mg·PPi are the specific substrates of H+-ATPase and H+-PPase respectively, hydrolysis H+ is pumped into vacuole, and contribution to build electrochemical proton gradient between cytoplasm and vacuole. N03--N transport from vacuole to cytoplasm greatly depends on electrochemical proton gradient, N03--N transport from cytoplasm to vacuole is mainly achieved by vacuole H+/N03-antiport system, while symport system (vacuole N03--N combined with anion) is of benefit for vacuole N03--N transporting into cytoplasm. N03--N transported by proton pump of tonoplast is influenced by NR activity in cytoplasm, N03--N can be continuing assimilation and reduction by NR in cytoplasm, and accelerating vacuole N03--N transported into cytoplasm. These results will supply references and research forecast for further study on efficiency and practicable methods of N utilization, and improving reuse efficiency of N03--N in plant tissues.
