轻量化深度学习模型的田间昆虫自动识别与分类算法
作者：
袁哲明;袁鸿杰;言雨璇;刘双清;谭泗桥
期刊：
吉林大学学报(工学版) ,2020年EICSCD ISSN：1671-5497
作者机构：
[刘双清] 湖南农业大学植物保护学院;湖南农业大学湖南省农业大数据分析与决策工程技术研究中心;湖南省农村农业信息化工程技术研究中心;湖南农业大学信息科学与技术学院;[谭泗桥; 言雨璇] 湖南省农村农业信息化工程技术研究中心<&wdkj&>湖南农业大学信息科学与技术学院
关键词：
模式识别技术;目标检测;昆虫分类;轻量化算法;图像预处理
摘要：
田间昆虫自动识别与分类是模式识别技术的一个重要方向，由于田间昆虫环境变化的随机性，以及田间昆虫类别之间样本数量的不均衡性，使得当前田间昆虫自动识别与分类方法存在误识率高、效率等难题，以获得更加理想的田间昆虫自动识别与分类效果为目标，提出了轻量化深度学习模型的田间昆虫自动识别与分类算法。首先对田间昆虫图像进行预处理，将其输入到轻量算法中进行特征提取，多尺度特征融合输出不同尺寸的预测网络，然后引入联合交并比进行田间昆虫自动识别与分类，最后与其算法进行了仿真对比实验。结果表明，本文算法的田间昆虫自动识别与分类正确率高，田间昆虫自动的误识率低，田间昆虫自动识别与分类时间少，可以对田间昆虫进行实时、在线识别，可以当前病虫害预测预报地需求，相对于对比算法，本文算法获得了整体性能更优的田间昆虫自动识别与分类结果，而且能够有效解决昆虫堆积、背景干扰问题，具有较强的鲁棒性。
语种：
中文
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Biocontrol Potential of Acinetobacter johnsonii ZY86 against Gray Mold by Botrytis cinerea
作者：
Zhang, Ya;Liu, Shuangqing* ;Wang, Chong;Liao, Xiaolan*
期刊：
International Journal of Agriculture and Biology ,2019年22(6):1467-1475 ISSN：1560-8530
通讯作者：
Liu, Shuangqing;Liao, Xiaolan
作者机构：
[Zhang, Ya; Liu, Shuangqing; Liao, Xiaolan; Liao, XL] Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;[Liu, Shuangqing] Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.;[Wang, Chong] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Liao, Xiaolan] Hunan Biol Pesticides & Pesticide Preparat Proc E, Changsha, Hunan, Peoples R China.
通讯机构：
[Liu, SQ; Liao, XL; Liu, Shuangqing; Liao, Xiaolan] H;Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.;Hunan Biol Pesticides & Pesticide Preparat Proc E, Changsha, Hunan, Peoples R China.
关键词：
Active components;Antagonistic bacteria;Botrytis cinerea;Control effect;Identification
摘要：
To resolve the problem of pesticide resistance in strawberry gray mold Botrytis cinerea and to improve the effectiveness of disease preventive measures by developing biological pesticides, a bacterial strain was identified using plate confrontation culture method, morphological characteristics, physiological and biochemical analyses, and 16S rDNA gene sequence analysis. The antifungal components of the antagonistic bacterial strain were analyzed by GC-MS, and their in vitro control effects were evaluated. One hundred bacterial strains were isolated from the strawberry rhizosphere soil, of which one showed superior antagonistic effect, accounting for 1% of all of the strains, and it was designated as strain ZY86. Further investigation indicated that strain ZY86 exhibited antifungal effects not only against B. cinerea but also against Rhizoctonia solani, Magnaporthe grisea, Colletotrichum capsici, Phytophthora capsici and Sclerotinia sclerotiorum. Strain ZY86 (GenBank Accession number MK111627) was highly similar to model strain Acinetobacter johnsonii (ATCC17909), with a homologous identity of 99%, and thus, strain ZY86 was identified as A. johnsonii. Analyses of the antifungal components showed that the active components produced by strain ZY86 were primarily amines, accounting for 20.80% of the total active components, which were followed, in order, by ketones, acids, esters, alcohols, peptides, hydrocarbons, alkanes, aldehydes, and alkenes in terms of proportion. In vitro testing showed that the control effect of strain ZY86 on strawberry gray mold was 84.14%, and higher the concentration, the better the control effect. In addition, strain ZY86 imparted a strong control effect against strawberry gray mold, and therefore, it is a promising biocontrol agent for agricultural production. (C) 2019 Friends Science Publishers
语种：
英文
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一种稻田水直供式植保混药装置
发明/设计人：
刘双清;陈斌;陈盛
申请/专利权人：
湖南农业大学
申请/专利号：
CN201910963947.5
申请时间：
2019-10-11
公开号：
CN110622943A
公开时间：
2019-12-31
主申请人地址：
410128 湖南省长沙市芙蓉区农大路1号
摘要：
本发明公开了一种稻田水直供式植保混药装置，包括储药容器和泥水细化机，泥水细化机的进口端连接有用于将田间的水抽吸至泥水细化机中的抽吸泵，泥水细化机的出口端通过送水管与储药容器的进口端相连，送水管连接有用于向送水管中加入药剂原液的加药组件，储药容器的出口端连接有出药管，出药管上设有输出泵。采用该稻田水直供式植保混药装置可降低植保机械整机重量、提高植保机械行驶稳定性、提高植保作业效率。
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高校寝室文化建设的现状及对策
作者：
余瑾;刘双清;黄春来
期刊：
管理观察 ,2019年(04):138-139 ISSN：1674-2877
作者机构：
[余瑾; 黄春来; 刘双清] 湖南农业大学
关键词：
高校;校园文化;寝室文化;现状与问题
摘要：
高校寝室文化是校园文化的重要组成部分,是反映高校特色、提高育人实效的重点工程。随着时代的进步,新时期高校的发展对寝室文化建设提出了更高的要求。本文通过阐释高校寝室文化的内涵和功能,分析了当前高校寝室文化建设存在的现状与问题,并对新形势下加强高校寝室文化建设提出了指导性意见。
语种：
中文
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Sensitivity and Cross-Resistance of Botrytis cinerea (Cause of Strawberry Grey Mould) to Three New Fungicides in Hunan Province, China
作者：
Zhang, Ya;Liu, Qing;Wang, Chong* ;Liu, Shuangqing;Shi, Li;...
期刊：
International Journal of Agriculture and Biology ,2019年22(6):1438-1444 ISSN：1560-8530
通讯作者：
Liao, Xiaolan;Wang, Chong
作者机构：
[Zhang, Ya; Liu, Shuangqing; Liu, Qing; Shi, Li; Liao, Xiaolan] Hunan Agr Univ, Coll Plant Protect, Dept Plant Protect, Changsha, Hunan, Peoples R China.;[Wang, Chong] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Liao, Xiaolan] Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.
通讯机构：
[Liao, Xiaolan; Wang, Chong] H;Hunan Agr Univ, Coll Plant Protect, Dept Plant Protect, Changsha, Hunan, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.
关键词：
Botrytis cinerea;Cross-resistance;Mancozeb;New fungicides;Sensitivity
摘要：
This study aimed to clarify the sensitivity and cross-resistance of Botrytis cinerea on strawberries to fluazinam (FAN), boscalid (BCI) and phenazine-1-carboxylic acid (PCA) to guide the rational use of these drugs. By measuring the mycelial growth rate, the sensitivity of 126 strains of B. cinerea on strawberries to FAN, BCI and PCA in 9 regions of Hunan Province was determined. The results showed that highest half maximal effective concentration (EC50) values for FAN, BCI and PCA against B. cinerea on strawberries were 0.9840 mu g/mL, 9.0564 mu g/mL and 11.2239 mu g/mL, respectively; the minimum values were 0.0135 mu g/mL, 0.1347 mu g/mL and 1.3540 mu g/mL, respectively, while the mean values were 0.1349 +/- 0.1710 mu g/mL, 2.1238 +/- 1.9095 mu g/mL and 5.2601 +/- 2.2908 mu g/mL, respectively. The highest values were 70.22-, 73.15- and 8.29-times higher, respectively, than the minimum values. The sensitivity frequencies of the three fungicides against B. cinerea on strawberries were not normally distributed, suggesting that sub-populations with reduced sensitivity were present in the B. cinerea population. Cross resistance to FAN, BCI and PCA existed. Field experiment results showed that the best control of B. cinerea occurred with FAN at 84.87%, which was 1.28-times that of the control, followed by BCI. PCA had the lowest control effect, which was lower than that of the control drug. This study provides a basis for decision-making in the future development of new policies for the prevention and control of B. cinerea. (C) 2019 Friends Science Publishers
语种：
英文
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强稳定性棉铃虫核型多角体病毒G4突变株的构建
作者：
刘双清;黄国华
作者机构：
湖南农业大学植物保护学院,湖南长沙410128
会议名称：
第十一届全国青年植保科技创新学术研讨会
会议时间：
20161207
会议地点：
南京
会议论文集名称：
第十一届全国青年植保科技创新学术研讨会论文集
关键词：
棉铃虫;多角体病毒;基因突变株;强稳定性
摘要：
鉴于野生型棉铃虫核型多角体病毒(HaSNPV)G4毒株的fp25k基因中含有的两个7A单核苷酸重复(mono-nucleotide repeats,MNRs)结构易突变为8A,致使病毒粒子致病力丧失,导致其在传代过程中极不稳定.本研究以fp25k基因未发生突变的野生型G4毒株的病毒粒子为实验材料,通过构建含fp25k基因的质粒,进行两次定点沉默突变,再通过构建含荧光蛋白的质粒,进行共转染、荧光筛选和空斑纯化得到消除了MNRs结构的强稳定性、具有持续维持强致病力特点的G4突变株.
语种：
中文
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一种稻田洁水农药喷施装置
发明/设计人：
刘双清;陈斌;陈盛
申请/专利权人：
湖南农业大学
申请/专利号：
CN201910963310.6
申请时间：
2019-10-11
公开号：
CN110574748A
公开时间：
2019-12-17
主申请人地址：
410128 湖南省长沙市芙蓉区农大路1号
摘要：
本发明公开了一种稻田洁水农药喷施装置，包括清水箱、药物原液储存箱、已配置药剂箱和用于从田间取水并过滤获得清水的过滤组件，过滤组件通过第一输水组件与清水箱相连，清水箱通过第二输水组件与已配置药剂箱相连，药物原液储存箱通过输药组件与已配置药剂箱相连。采用该稻田洁水农药喷施装置可降低植保机械整机重量、提高植保机械行驶稳定性、提高植保作业效率。
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一种稻田水直供式植保混药装置
发明/设计人：
刘双清;陈斌;陈盛
申请/专利权人：
湖南农业大学
申请/专利号：
CN201921702942.9
申请时间：
2019-10-11
公开号：
CN210960082U
公开时间：
2020-07-10
主申请人地址：
410128 湖南省长沙市芙蓉区农大路1号
摘要：
本实用新型公开了一种稻田水直供式植保混药装置，包括储药容器和泥水细化机，泥水细化机的进口端连接有用于将田间的水抽吸至泥水细化机中的抽吸泵，泥水细化机的出口端通过送水管与储药容器的进口端相连，送水管连接有用于向送水管中加入药剂原液的加药组件，储药容器的出口端连接有出药管，出药管上设有输出泵。采用该稻田水直供式植保混药装置可降低植保机械整机重量、提高植保机械行驶稳定性、提高植保作业效率。
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一种稻田洁水农药喷施装置
发明/设计人：
刘双清;陈斌;陈盛
申请/专利权人：
湖南农业大学
申请/专利号：
CN201921700433.2
申请时间：
2019-10-11
公开号：
CN210960081U
公开时间：
2020-07-10
主申请人地址：
410128 湖南省长沙市芙蓉区农大路1号
摘要：
本实用新型公开了一种稻田洁水农药喷施装置，包括清水箱、药物原液储存箱、已配置药剂箱和用于从田间取水并过滤获得清水的过滤组件，过滤组件通过第一输水组件与清水箱相连，清水箱通过第二输水组件与已配置药剂箱相连，药物原液储存箱通过输药组件与已配置药剂箱相连。采用该稻田洁水农药喷施装置可降低植保机械整机重量、提高植保机械行驶稳定性、提高植保作业效率。
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新形势下地方农业高校科技工作的重要使命、发展思路与实现途径
作者：
刘双清
期刊：
科技成果管理与研究 ,2019年(5):23-26 ISSN：1673-6516
作者机构：
湖南农业大学科学技术处,湖南 长沙,410128;[刘双清] 湖南农业大学
关键词：
农业高校;科技工作;重要使命;发展思路;实现途径
摘要：
实施创新驱动发展、"双一流"高校建设和服务支撑乡村振兴,是新形势下地方农业高校科技工作的重要任务.文章分析了新形势下地方农业高校科技工作的重要使命,探讨了推进地方农业高校科技工作的发展思路以及实现途径.
语种：
中文
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氟啶胺和咯菌腈复配对草莓灰霉病菌的联合毒力及增效作用研究
作者：
刘青;张亚;刘双清;廖晓兰
期刊：
中国果树 ,2019年(2):43-47 ISSN：1000-8047
作者机构：
湖南农业大学植物保护学院,长沙410128;[廖晓兰] 湖南农业大学东方科技学院;植物病虫害生物学与防控湖南省重点实验;湖南省生物农药与农药制剂加工工程技术研究中心;[张亚; 刘青; 刘双清] 湖南农业大学
关键词：
氟啶胺;咯菌腈;草莓灰霉病菌;联合毒力;增效作用
摘要：
为了明确氟啶胺和咯菌腈及其复配物对草莓灰霉病菌的室内毒力,研制新的防治草莓灰霉病药剂配方并明确其田间防效。采用生长速率法研究了氟啶胺、咯菌腈及其复配物对草莓灰霉病菌的室内毒力,以Wadley法进行增效作用评价,并验证该复配物对草莓灰霉病的田间防效。结果表明:氟啶胺和咯菌腈以质量比1∶5、3∶2复配对草莓灰霉病菌具有增效作用,其增效系数分别为1.82和2.07;氟啶胺和咯菌腈以质量比1∶1、1∶3、2∶3、2∶1、3∶1、4∶1复配对草莓灰霉病菌具有相加作用,但以质量比5∶1复配时,则表现拮抗作用;氟啶胺和咯菌腈以3∶2复配对草莓灰霉病的田间防效最高可达91.00%,比使用单一化学药剂效果好,而且比对照药剂高。综上,氟啶胺和咯菌腈及其复配物对草莓均无药害,且对草莓生长无影响;氟啶胺与咯菌腈以3∶2进行复配,可以在草莓生产上推广使用。
语种：
中文
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动植物检疫专业英语学习调查研究
作者：
张亚;甘璐;刘双清;廖晓兰
期刊：
农业教育研究 ,2019年(02):27-29
作者机构：
湖南农业大学,湖南长沙410128;植物病虫害生物学与防控湖南省重点实验室,湖南长沙410128;湖南省生物农药与农药制剂加工工程技术研究中心,湖南长沙410128
关键词：
专业英语;问题;调查;措施
摘要：
为提高动植物专业英语学习质量,掌握听说读写译的技巧,笔者根据自身经验并结合问卷的方式,分别从专业英语的重要性、学习之困、提高专业英语措施以及学习后的收获等方面调查了动植物检疫专业2013级、2014级、2015级本科生。结果表明,98.08%的学生认为学习专业英语非常重要,1.92%的学生认为不重要;学习专业英语困难有多种,例如单词量大,生僻,不常用,单词长等是提高专业英语学习能力的主要障碍;学生普遍认为多听专业英语学术报告,多阅读英文论文、课堂上师生一起学、敢于张开口等措施有助于提高专业英语综合能力。
语种：
中文
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Fungicidal activity and biological characteristics of a novel natural product fungicide: Phenazine-1-carboxamide-derived 18-1
作者：
Zhang, Ya;Liu, Shuangqing* ;Wang, Chong;Liao, Xiaolan*
期刊：
International Journal of Agriculture and Biology ,2018年20(11):2378-2386 ISSN：1560-8530
通讯作者：
Liu, Shuangqing;Liao, Xiaolan
作者机构：
[Zhang, Ya; Liu, Shuangqing; Liao, Xiaolan; Liao, XL] Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;[Liu, Shuangqing] Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.;[Wang, Chong] Hunan Agr Univ, Sci Coll, Changsha, Hunan, Peoples R China.;[Liao, Xiaolan] Hunan Biol Pesticides & Pesticide Preparat Proc E, Changsha, Hunan, Peoples R China.
通讯机构：
[Liu, SQ; Liao, XL; Liu, Shuangqing; Liao, Xiaolan] H;Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.;Hunan Biol Pesticides & Pesticide Preparat Proc E, Changsha, Hunan, Peoples R China.
关键词：
Carboxamidebiological characteristics;Derivative;Fungicidal activity;Phenazine-1-carboxamide;Plant pathogenic fungus;Rhizoctonia solani
摘要：
A novel phenazine-l-carboxamide-derived 18-1 (PCND 18-1) was evaluated in terms of its potential for the development and enrichment of new biofungicides for the control of rice sheath blight caused by Rhizoctonia solani. PCND 18-1 exhibited fungicidal activity against R. solani, showing an inhibition rate of 87.64%, with a 50% effective concentration (EC50) 4.25 mu g/mL, regression equation of Y = 0.7105x + 3.8428; and correlation coefficient of 0.9817, which are indicative of its potential as a natural biofungicide. PCND 18-1 also attenuated the pathogenicity of R. solani, which was concentration-dependent. Additionally, the action mode of PCND 18-1 against rice sheath blight (R. solani) was protective better than curative activity under greenhouse condition, as reflected by the corresponding EC50 values of 2.49 and 5.72 mu g/mL. PCND 18-1 can translocate in rice with a low translocation capacity and exhibited a higher capacity for upward (root-leaf) translocation than for downward (leaf-root) translocation. Furthermore, PCND 18-1 demonstrated adhesion to leaves but poor tolerance against rain-washing. The optimal persistence period of PCND 18-1 on rice was 7 d. (C) 2018 Friends Science Publishers
语种：
英文
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A New Disease of Strawberry, Fruit Rot, Caused by Geotrichum candidum in China
作者：
Ma, Wenyue;Zhang, Ya* ;Wang, Chong;Liu, Shuangqing;Liao, Xiaolan*
期刊：
PLANT PROTECTION SCIENCE ,2018年54(2):92-100 ISSN：1212-2580
通讯作者：
Zhang, Ya;Liao, Xiaolan
作者机构：
[Zhang, Ya; Liu, Shuangqing; Ma, Wenyue; Zhang, Y; Liao, XL; Liao, Xiaolan] Hunan Agr Univ, Coll Plant Protect, Dept Plant Protect, Changsha, Hunan, Peoples R China.;[Wang, Chong] Hunan Agr Univ, Sci Coll, Dept Chem, Changsha, Hunan, Peoples R China.;[Liao, Xiaolan] Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.
通讯机构：
[Zhang, Y; Liao, XL; Liao, Xiaolan] H;Hunan Agr Univ, Coll Plant Protect, Dept Plant Protect, Changsha, Hunan, Peoples R China.;Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.
关键词：
Fragaria ananassa Duch.;new pathogenic fungi;Geotrichum candidum;identification;rDNA-ITS
摘要：
A new disease of strawberry (Fragaria ananassa Duch.) was discovered in the Lianqiao strawberry planting base in Shaodong County, in Hunan Province, China. In the early disease stage, leaves showed small black spots surrounded by yellow halos, while in the late stage, a white fluffy layer of mold appeared. Fruits were covered with a white layer of mold. The symptoms were observed using in vitro inoculation experiments. After the spray-inoculation of stabbed leaves, small black spots surrounded by yellow halos occurred on leaves, with no clear boundary between diseased and healthy areas. In the late stage, disease spots gradually expanded and a white fluffy layer of mold formed under humid conditions. Unstabbed leaves had almost no disease occurrence after spray-inoculation. After the spray-inoculation of stabbed fruits, by the late stage, a dense white layer of mold formed. According to Koch’s postulates, the isolated strain was verified as a pathogen. The pathogenic strain, designated SDLQ16, was isolated from diseased fruit by dilution method and tentatively identified as G. candidum based on the culture characteristics, morphologies, physio-biochemical analysis, and phylogenetic analysis of the rDNA-ITS sequence. The fungus was able to grow on different culture mediums, with a broad range of nutrition. The colonies on PDA medium were raised and pale white, with a neat edge and visible hyphae. The hyphae were friable but the spores were developing. Basal hyphae rapidly grew close to the medium to 3.2–4.2 µm in diameter, with septa and forked branches at acute angles. The solitary or beaded spores with smooth surfaces were 3.5–7.5 µm in length and 3.5–4.5 µm in width. This strain was able to gelatin liquefaction, proteolysis, grease, peptonised milk, urea, and so on. The pathogenicity on strawberry from strong to weak was: fruit > leaf > stem. A BLAST algorithm was used to query SDLQ16’s rDNA-ITS sequence (cloned and deposited as GenBank number KU373122) against the NCBI database, and it was located in the Acinetobacter sp. branch of a phylogenetic tree. SDLQ16 was most closely related to Geotrichum candidum ATCC34614 (GQ4580314.1), with a sequence similarity of 99%. © 2018, Czech Academy of Agricultural Sciences. All rights reserved.
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英文
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热激转录因子在植物抗非生物胁迫中的功能研究进展
作者：
李丽;刘双清;杨远航;戴良英;李魏
期刊：
生物技术进展 ,2018年8(3):214-220 ISSN：2095-2341
作者机构：
湖南农业大学植物保护学院,作物基因工程湖南省重点实验室,长沙410128;[李魏; 戴良英; 杨远航; 李丽; 刘双清] 湖南农业大学
关键词：
热激转录因子;非生物胁迫;高温;干旱
摘要：
植物在遭受环境胁迫时会产生一系列应激反应,而热激转录因子可通过介导热激蛋白或其他热诱导基因的转录和表达,来参与调控植物抵抗逆境胁迫过程和其他生命活动。主要介绍了植物热激转录因子的基本蛋白结构域,阐述了3类热激转录因子在抗极端温度（高温、低温）胁迫、干旱胁迫、高盐胁迫、活性氧胁迫中的功能与作用机制,并探讨和展望了植物热激转录因子在植物育种和提高植物抗逆性的研究中的发展与应用前景,以期为深入研究热激转录因子在调控植物抵抗逆境胁迫中的生物学功能与机制提供理论参考。
语种：
中文
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Effects and inhibition mechanism of phenazine-1-carboxamide on the mycelial morphology and ultrastructure of Rhizoctonia solani
作者：
Xiang, Yaqin;Zhang, Ya* ;Wang, Chong;Liu, Shuangqing;Liao, Xiaolan*
期刊：
Pesticide Biochemistry and Physiology ,2018年147:32-39 ISSN：0048-3575
通讯作者：
Zhang, Ya;Liao, Xiaolan
作者机构：
[Zhang, Ya; Liu, Shuangqing; Xiang, Yaqin; Zhang, Y; Liao, XL; Liao, Xiaolan] Hunan Agr Univ, Dept Plant Protect, Coll Plant Protect, Changsha, Hunan, Peoples R China.;[Wang, Chong] Hunan Agr Univ, Dept Chem, Sci Coll, Changsha, Hunan, Peoples R China.;[Liao, Xiaolan] Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.
通讯机构：
[Zhang, Y; Liao, XL] H;Hunan Agr Univ, Dept Plant Protect, Coll Plant Protect, Changsha, Hunan, Peoples R China.
摘要：
The purpose of this research was to explore the effect of phenazine-1-carboxamide (PCN) on Rhizoctonia solani and to elucidate its mechanisms of action. The toxicity of PCN to R. solani was measured using a growth rate method. The results indicated that PCN inhibited R. solani with a 50% effective concentration (EC50) of 9.0934 μg/mL. The mycelia of R. solani were then exposed to 18.18 μg/mL (2EC50) of PCN. Optical microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM) were used to observe the effects of PCN on mycelial morphology and ultrastructure. Following the PCN treatment, the optical microscopy observations revealed that the mycelia appeared twisted; the branching mycelia grew, but the main mycelia did not grow following branching; and the mycelial roots possessed more vacuoles. SEM observations revealed that the mycelia were locally swollen and exhibited a sharp decrease in prominence. TEM observations showed that the cell wall became thin and deformed; the mitochondria disappeared; the septum twisted; and most of the organelles were difficult to discern. Conversely, all of the organelles could be clearly observed in the control. We then used real-time quantitative PCR and an enzyme activity testing kit to further explore the effects of PCN on the cell wall and mitochondria. Physiological and biochemical results demonstrated that both the cell wall and mitochondria constitute are PCN targets. PCN inhibited the activities of chitin synthetase and complex I of the mitochondria electron transport chain. Molecular experiments demonstrated that PCN controlled the growth of R. solani mycelia by inhibiting the expression level of chitin synthetase genes. Future research on PCN should investigate its influence on metabolic pathways, thereby aiding in the potential development of novel pesticides. © 2017 Elsevier Inc.
语种：
英文
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“双一流”建设背景下地方高校加强科技创新的分析与对策
作者：
刘双清;陈光辉;刘秋成
期刊：
科技成果管理与研究 ,2018年(2):20-22,33 ISSN：1673-6516
作者机构：
湖南农业大学科学技术处,湖南 长沙,410128;湖南生物机电职业技术学院经济贸易学院,湖南 长沙,410127;[陈光辉; 刘双清] 湖南农业大学;[刘秋成] 湖南生物机电职业技术学院
关键词：
"双一流"建设;地方高校;科技创新
摘要：
建设世界一流大学和一流学科,是提升我国高等教育发展水平、增强国家核心竞争力的重要举措,而科技创新正是建设一流大学和一流学科的重要支撑.该文从建设世界科技强国、建设创新型湖南、建设"双一流"高校等三个维度分析了科技创新的重要性,从优化资源配置、营造优越环境、重视基础研究、加强组织领导等几个方面提出了地方高校加强科技创新的有效途径.
语种：
中文
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科技报告制度建设的现状分析及提升对策研究
作者：
刘双清;杨友才;陈光辉
期刊：
科技成果管理与研究 ,2018年(10):24-26,35 ISSN：1673-6516
作者机构：
湖南农业大学科学技术处,湖南 长沙,410128;[杨友才; 陈光辉; 刘双清] 湖南农业大学
关键词：
科技报告;制度建设;科技管理;现状;对策
摘要：
撰写科技报告是科技项目研究实施的重要组成部分和关键环节，科技报告制度建设是科技项目全过程管理中一项重要的、长期的工作任务。文章结合高校科技管理工作的实践体会，从分析科技报告制度建设的重要意义出发，剖析了科技报告制度建设过程中的几个问题，进而探讨了推动科技报告制度建设的关键环节，并提出了推进科技报告制度建设的对策和建议。
语种：
中文
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新形势下加强高校科研经费管理的分析与对策研究
作者：
刘双清;张铭辉;伍小松;陈光辉
作者机构：
湖南农业大学 科学技术处,长沙 410128;湖南农业大学 计划财务处,长沙 410128
会议名称：
第六届农林高校哲学社会科学发展论坛
会议时间：
201511
会议地点：
广州
会议论文集名称：
第六届农林高校哲学社会科学发展论坛论文集
关键词：
高等院校;科研经费;分级管理;监督机制
摘要：
科学高效管理科研经费,是提升高校科研经费管理水平,提高科研经费使用效益,促进高校科研事业发展的重要抓手.本文从分析高校科研经费管理工作中存在的问题出发,从明确责任主体、建立分级管理机制,完善工作机制、提升管理服务能力,加强统一管理、严格科研经费支出,加强监督检查、推进财务信息公开等四个方面探讨了新形势下高校科研经费管理的有效对策,并介绍了湖南农业大学加强科研经费管理的有关做法与措施.
语种：
中文
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Distribution and degradation kinetics of cyhalodiamide in Chinese rice field environment
作者：
Liu, Yiping;Zhang, Ya;Liu, Shuangqing;Lu, Yuntao;Lin, Ronghua;...
期刊：
中国化学工程学报(英文版) ,2018年26(10):2185-2191 ISSN：1004-9541
通讯作者：
Liao, Xiaolan;Li, Xiaogang
作者机构：
[Zhang, Ya; Liu, Shuangqing; Li, Xiaogang; Liao, XL; Li, XG; Liao, Xiaolan; Liu, Yiping] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Lu, Yuntao; Liu, Yiping] Inst Control Agrochem, Changsha 410005, Hunan, Peoples R China.;[Lin, Ronghua; Li, Min] Minist Agr & Rural Affairs, Inst Control Agrochem, Beijing 100125, Peoples R China.
通讯机构：
[Liao, XL; Li, XG] H;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.
关键词：
Cyhalodiamide;QuEChERS;Rice;Residue;Degradation kinetics
摘要：
Cyhalodiamide is a novel agrochemical which is effective against Lepidoptera pests, including Cnaphalocrocis medinalis, Chilo suppressalis, Pieris rapae, Plutella xylostella, Helicoverpa armigera, etc. In the study, a fast and accurate analytical method was developed to detect cyhalodiamide in Chinese typical rice field environment by a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) method with UPLC-MS/MS (ultra-high performance chromatography-tandem mass spectrometry). The mean recoveries of cyhalodiamide varied from 73.5% to 107.5%, with the RSDs from 1.2% to 10.7%. The limits of determination (LODs) were 0.0005 mg·kg− 1, and the limits of quantitation (LOQs) were from 0.002 to 0.01 mg·kg− 1 in all five matrices. This method was used to determine cyhalodiamide residues for studies of the distribution and degradation kinetics in rice field environment. The field trials results showed that cyhalodiamide was easily degradable and the half-lives were 4.2–13.6 d in rice straw, 8.77 d in paddy soil and 5.37–8.45 d in paddy water, respectively. The final residues of cyhalodiamide in brown rice were below 0.35 mg·kg− 1. The used dosage of 33.75 g·hm− 2 with pre-harvest interval (PHI) of 21 d and the maximum residue limit (MRL) of cyhalodiamide in rice at 0.1 mg·kg− 1 were recommended, which would be safe to human health and environment. The developed analytical method will be useful to monitor cyhalodiamide residues and safety evaluation in rice environment. © 2018 Elsevier B.V.
语种：
英文
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