北美车前提取液对稗草和千金子的化感作用
作者：
张家迪;刘双清;邬腊梅;周尚峰;黄晨;...
期刊：
草地学报 ,2024年32(2):510-516 ISSN：1007-0435
作者机构：
[张家迪] 湖南农业大学植物保护学院,湖南 长沙 410128;[周尚峰; 杨浩娜#&#&#ZHANG Jia-di; 张家迪; 邬腊梅] 湖南省农业科学院杂草生物学及安全防控湖南省重点实验室,湖南 长沙 410125;[黄晨; 刘双清] 湖南农业大学
关键词：
北美车前;化感作用;植物源除草剂;稗草;千金子;腐解
摘要：
从植物中筛选出具有除草活性的化感物质为植物源除草剂的开发提供了丰富的资源.本试验比较分析了北美车前(Plantagovirginica)水提液、醇提液与腐解提取液对稗草(Echinochloa crusgalli)和千金子(Leptochloa chinensis)种子萌发的化感作用.并利用超高效液相色谱-质谱联用(UHPLC-QE-MS)技术鉴定腐解提取液中的主要化感物质,评价各物质对稗草和千金子种子萌发的影响.结果显示:腐解提取液对稗草和千金子种子萌发的抑制效果最强,壬酸、正辛酸、4-甲基儿茶酚为主要化感物质.4-甲基儿茶酚处理后稗草超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性随处理浓度增加先增强又下降.而相同处理下的千金子SOD与POD活性随试验浓度增高先降低后增高,CAT活性则随试验浓度增大而增强,表明4-甲基儿茶酚处理干扰了杂草体内活性氧平衡,且对稗草和千金子抗氧化酶的影响不同.
语种：
中文
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Comprehensive analysis of the overexpressed cytochrome P450-based insecticide resistance mechanism in Spodoptera litura
作者：
Li, Wenlin;Yang, Wen;Shi, Yao;Yang, Xiyu;Liu, Shuangqing;...
期刊：
Journal of Hazardous Materials ,2024年461:132605 ISSN：0304-3894
通讯作者：
Liao, Xiaolan;Shi, L
作者机构：
[Li, Wenlin; Yang, Wen; Liu, Shuangqing; Shi, Li; Yang, Xiyu; Liao, Xiaolan; Shi, Yao; Liao, XL] Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China.
通讯机构：
[Shi, L ; Liao, XL] H;Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China.
关键词：
Cross-resistance;Functional validation;Insecticide resistance;P450s;Spodoptera litura
摘要：
Cytochrome P450s play critical roles in the metabolic resistance of insecticides in insects. Previous findings showed that enhanced P450 activity was an important mechanism mediating indoxacarb resistance, and multiple P450 genes were upregulated in indoxacarb resistant strains of Spodoptera litura. However, the functions of these P450 genes in insecticide resistance remain unknown. Here, the P450 inhibitor PBO effectively decreased the resistance of S. litura to indoxacarb. Ten upregulated P450 genes were characterized, all of which were overexpressed in response to indoxacarb induction. Knockdown of nine P450 genes decreased cell viability against indoxacarb, and further silencing of three genes (CYP339A1, CYP340G2, CYP321A19) in larvae enhanced the sensitivity to indoxacarb. Transgenic overexpression of these three genes increased resistance to indoxacarb in Drosophila melanogaster. Moreover, molecular modeling and docking predicted that these three P450 proteins could bind tightly to indoxacarb and N-decarbomethoxylated metabolite (DCJW). Interestingly, these three P450 genes may also mediate cross-resistance to chlorantraniliprole, λ-cyhalothrin and imidacloprid. Additionally, heterologous expression and metabolic assays confirmed that three recombinant P450s could effectively metabolize indoxacarb and DCJW. This study strongly demonstrates that multiple overexpressed mitochondrial and microsomal P450 genes were involved in insecticide resistance in S. litura.
语种：
英文
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Integrated histopathology and transcriptome metabolome profiling reveal the toxicity mechanism of phenazine-1-carboxylic acid in zebrafish
作者：
Zhang, Ya;Zeng, Hao;Zhou, Leyin;Wang, Chong;Yang, Xiao;...
期刊：
Environmental Pollution ,2024年344:123402 ISSN：0269-7491
通讯作者：
Shuangqing Liu
作者机构：
[Zhang, Ya; Zhou, Leyin; Zeng, Hao] College of Plant Protection, Hunan Agricultural University, Changsha 410128, China;[Wang, Chong] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;[Yang, Xiao] Key Laboratory of Land Surface Pattern and Simulation, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;[Liu, Shuangqing] College of Plant Protection, Hunan Agricultural University, Changsha 410128, China. Electronic address: liushuangqing@hunau.edu.cn
通讯机构：
[Shuangqing Liu] C;College of Plant Protection, Hunan Agricultural University, Changsha 410128, China
关键词：
Omics;Pathology;Phenazine-1-carboxylic acid;Toxicology;Zebrafish
摘要：
Phenazine-1-carboxylic acid (PCA) is a new type of agrochemical used to prevent plant diseases, but its effects on aquatic organisms are unclear. To comprehensively assess the impacts of PCA for aquatic organisms and its associated environmental risks, this study investigated, taking zebrafish as the research object, the toxicological mechanism of PCA by means of optical microscopy, hematoxylin and eosin (HE) staining, ultrastructural observation, physiological and biochemical testing, transcriptome sequencing, metabolome analysis, fluorescence quantitative PCR and molecular simulation. The results indicated that PCA was detrimental to zebrafish embryos, larvae and adults, with LC(50) values at 96h of 3.9093mg/L, 8.5075mg/L, and 13.6388mg/L, respectively. PCA caused abnormal spontaneous movement, slowed the heart rate, delayed hatching, shortened the body length, slowed growth, and caused malformations. PCA mainly affected the brain, liver, heart, and ovaries. PCA distorted cell morphology, damaged mitochondrial membranes, disintegrated mitochondrial ridges, and dissociated nuclear membranes. PCA inhibited the enzyme activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX), decreased the malondialdehyde (MDA) content and disrupted antioxidant effects. The results of omics studies confirmed that PCA interfered with the transcriptional and metabolic network of zebrafish, downregulating most genes and metabolites. PCA mainly affected functions related to mitochondrial steroids, lipids, sterols, oxidoreductase activity and pathways involving cofactors, steroids, porphyrin, cytochromes, which specifically bound to targets such as panx3, agmat, and ace2. PCA was moderately toxic to zebrafish, and its usage should be strictly controlled to reduce toxic effects on aquatic organisms. The results of this study provide a new insights for ecotoxicology research.
语种：
英文
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荧光标记技术在新型农用化合物创制中的应用现状及进展
作者：
文鼎;张亚;刘双清;王翀;廖晓兰
期刊：
河南农业科学 ,2023年52(09):1-13 ISSN：1004-3268
作者机构：
[张亚; 廖晓兰; 文鼎; 刘双清] 湖南农业大学植物保护学院;[王翀] 湖南农业大学生物科技学院
关键词：
荧光标记;农用化合物创制;荧光染料;荧光探针;免疫荧光
摘要：
荧光标记技术广泛应用于现代社会的各个方面，但其在农用化合物创制领域的应用鲜见综述。基于近10 a国内外农用化合物创制领域的荧光标记技术相关文献，系统总结了荧光标记技术中荧光染料的种类，综合介绍了荧光探针技术和免疫荧光技术在新型农用化合物创制领域的应用现状及进展，分析了目前荧光标记技术在新型农用化合物创制领域存在的局限与不足，并对未来荧光标记技术的发展趋势进行了展望。
语种：
中文
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湖南农业大学2011—2020年国家自然科学基金项目资助情况分析与发展对策探讨
作者：
刘双清;李杰;李莎
期刊：
科技成果管理与研究 ,2022年17(2):14-18 ISSN：1673-6516
作者机构：
湖南农业大学科学技术处,湖南 长沙 410128;[李杰; 李莎; 刘双清] 湖南农业大学
关键词：
国家自然科学基金;项目管理;发展战略;实现路径
摘要：
国家自然科学基金支持基础研究和应用基础研究,坚持自由探索,培养科技人才,在国内科学界享有很高的关注度和认可度.通过分析湖南农业大学2011—2020年国家自然科学基金项目的申请与资助情况以及项目研究实施的成果与绩效产出情况,总结学校在国家自然科学基金的组织申报与全过程管理的主要做法,提出了"十四五"期间加强基础研究创新能力建设的发展战略和实现路径.
语种：
中文
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世界农业科技发展趋势对湖南省农业农村发展的启示
作者：
刘双清;王俊琳;文鼎;吕军;黄大金
期刊：
科技成果管理与研究 ,2022年17(8):20-24 ISSN：1673-6516
作者机构：
[刘双清] 湖南农业大学科学技术处,湖南 长沙 410128;[刘双清] 湖南农业大学植物保护学院,湖南 长沙 410128;湖南省平江县农业农村局,湖南 平江 414500;湖南农业大学经济学院,湖南 长沙 410128;[吕军] 湖南省平江县农业农村局
关键词：
世界农业;农业科技;农业产业;农业农村
摘要：
科技驱动农业转型升级、乡村宜居宜业、农民富裕富足,是推进农业农村现代化和实现乡村振兴的必然要求.通过梳理世界农业发展的形势与趋势,分析世界农业科技发展的形势与趋势,探讨推动湖南省农业农村发展的战略与对策.
语种：
中文
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Hypovirulence caused by mycovirus in Colletotrichum fructicola
作者：
Guo, Jun;Zhou, Xinyu;Xie, Fangling;Cao, Junjie;Liu, Shuangqing;...
期刊：
FRONTIERS IN PLANT SCIENCE ,2022年13:1038781 ISSN：1664-462X
通讯作者：
Zhong, J.;Zhu, H.
作者机构：
[Xie, Fangling; Liu, Shuangqing; Zhong, Jie; Zhou, Xinyu; Cao, Junjie; Guo, Jun; Zhu, Hongjian] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Inse, Changsha, Peoples R China.
通讯机构：
[Zhong, J.; Zhu, H.] H;Hunan Provincial Key Laboratory for Biology, China
关键词：
Colletotrichum fructicola;CgOLV1-CfOLV1;CfOLV2;The integrity of the cell wall;Transcriptomic Analysis
摘要：
Colletotrichum fructicola is a pathogenic fungus causing leaf black spot and fruit rot disease in a wide variety of crops. Some mycoviruses that cause detrimental effects on fungal hosts could be useful in studying the pathogenesis of fungal hosts. In this study, we reported two mycoviruses, Colletotrichum fructicola ourmia-like virus 1- Colletotrichum gloeosporioides ourmia-like virus 1 (CfOLV1-CgOLV1) and Colletotrichum fructicola ourmia-like virus 2 (CfOLV2), from a C. fructicola fungus. The complete genome sequences of CfOLV1-CgOLV1 and CfOLV2 contain 2,516 bp and 2,048 bp, respectively. Both of these viruses contain only one open reading frame (ORF), which encodes an RNA-dependent RNA polymerase (RdRp). CfOLV1-CgOLV1 was identical as the previously reported virus CgOLV1. Phylogenetic analysis showed that CfOLV2 is closely related to Scleroulivirus and Magoulivirus in the family Botourmiaviridae. Virus elimination and horizontal transmission experiments proved that the associated mycoviruses could reduce the pathogenicity of the host C. fructicola. In addition, we found that the virus-containing strains showed a much higher percentage of appressorium formation and more melanin production compared to isogenic virus-free strain, and the presence of the virus is detrimental to the growth of host fungi and regulates the integrity of the cell wall. Transcriptomic analysis showed that mycovirus infection caused various abnormal genes expression in C. fructicola. To the best of our knowledge, this is the first report of a hypovirulence-associated ourmia-like mycovirus in C. fructicola.
语种：
英文
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Transcriptomic and metabolomic analyses reveal the antifungal mechanism of the compound phenazine-1-carboxamide on Rhizoctonia solani AG1IA
作者：
Zhang, Ya;Li, Qiufeng;Wang, Chong;Liu, Shuangqing
期刊：
FRONTIERS IN PLANT SCIENCE ,2022年13:1041733 ISSN：1664-462X
通讯作者：
Wang, C.;Liu, S.
作者机构：
[Zhang, Ya; Liu, Shuangqing; Li, Qiufeng] Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China.;[Wang, Chong] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Peoples R China.
通讯机构：
[Liu, S.; Wang, C.] C;College of Bioscience and Biotechnology, China;College of Plant Protection, China
关键词：
Phenazine-1-carboxamide;Rhizoctonia solani;Transcriptome;Differential genes;Metabolome;differential metabolites;Combined analysis
摘要：
To explore the molecular mechanisms of the antifungal compound phenazine-1-carboxamide (PCN) inhibits Rhizoctonia solani and discover potential targets of action, we performed an integrated analysis of transcriptome and metabolome in R. solani mycelium by whether PCN treating or not. A total of 511 differentially expressed genes (DEGs) were identified between the PCN treatment and control groups. The fluorescence-based quantitative PCR (qPCR) got the accordant results of the gene expression trends for ten randomly selected DEGs. The Gene Ontology (GO) enrichment analysis revealed that fatty acid metabolic process, fatty acid oxidation, and lipid oxidation were among the most enriched in the biological process category, while integral component of membrane, plasma membrane, and extracellular region were among the most enriched in the cellular component category and oxidoreductase activity, cofactor binding, and coenzyme binding were among the most enriched in the molecular function category. KEGG enrichment analysis revealed the most prominently enriched metabolic pathways included ATP-binding cassette (ABC) transporters, nitrogen metabolism, aminobenzoate degradation. The DEGs related functions of cellular structures, cell membrane functions, cellular nutrition, vacuole-mitochondrion membrane contact site and ATPase activity, pH, anti-oxidation, were downregulated. A total of 466 differential metabolites were found between the PCN treatment and control groups after PCN treatment. KEGG enrichment found purine, arachidonic acid, and phenylpropanoid biosynthesis pathways were mainly affected. Further results proved PCN decreased the mycelial biomass and protein content of R. solani, and superoxide dismutase (SOD) activity reduced while peroxidase (POD) and cytochrome P450 activities increased. The molecule docking indicted that NADPH nitrite reductase, ATP-binding cassette transporter, alpha/beta hydrolase family domain-containing protein, and NADPH–cytochrome P450 reductase maybe the particular target of PCN. In conclusion, the mechanisms via which PCN inhibits R. solani AG1IA may be related to cell wall damage, cell membrane impairment, intracellular nutrient imbalance, disturbed antioxidant system, and altered intracellular pH, which laid foundation for the further new compound designing to improve antifungal efficacy. Copyright © 2022 Zhang, Li, Wang and Liu.
语种：
英文
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高校科研成果转化为本科教学资源的改进途径和机制创新
作者：
刘双清;王俊琳;文鼎;张亚;廖晓兰
期刊：
吉林省教育学院学报 ,2022年38(8):142-145 ISSN：1671-1580
作者机构：
湖南农业大学植物保护学院;[廖晓兰; 张亚; 文鼎; 王俊琳; 刘双清] 湖南农业大学
关键词：
科研成果;本科教学;教学资源;改进途径;机制创新
摘要：
科技成果转化是高校科技工作的一项重要内容,将科研成果转化为优质本科教学资源,对于教学质量提升和人才培养提质至关重要.探究科研成果转化为教学资源的实现路径和保障机制,是高等教育教学改革需要解决的重要课题.本文分析了高校科研成果转化为本科教学资源的主要方式,探讨了转化过程中存在的问题和改进路径,提出了要建立和完善激励机制、评价机制和保障机制,通过机制创新实现科研成果转化为本科教学资源的长效机制,有效推动科研和教学的有机融合、互促互进.
语种：
中文
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微视频在植物病害检疫学实验课教学中的实践与评价
作者：
张亚;王翀;刘双清;廖晓兰
期刊：
中国现代教育装备 ,2022年(15):17-20 ISSN：1672-1438
作者机构：
[张亚; 廖晓兰; 刘双清] 湖南农业大学植物保护学院;[王翀] 湖南农业大学生物科技学院
关键词：
微视频;实验教学;实践;应用
摘要：
微视频在实验教学中的应用,极大地提高了实验教学质量.介绍了微视频的重要性,从微视频的准备、录制、编辑,以及微视频的评分指标和教学效果等方面进行本科实验教学实践,分析了实验教学中可能存在的问题及解决对策,旨在为今后一流本科专业教学改革提供参考.
语种：
中文
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A Novel Antibacterial Component and the Mechanisms of an Amaranthus tricolor Leaf Ethyl Acetate Extract against Acidovorax avenae subsp. citrulli
作者：
Zhang, Ya;Gao, Ke;Wang, Chong* ;Liu, Shuangqing*
期刊：
International Journal of Molecular Sciences ,2022年23(1) ISSN：1661-6596
通讯作者：
Liu, Shuangqing;Wang, Chong
作者机构：
[Zhang, Ya; Gao, Ke; Liu, Shuangqing] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China.;[Wang, Chong] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
通讯机构：
[Liu, Shuangqing; Wang, Chong] H;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
关键词：
Amaranthus tricolor;bacteriostatic component;identification;ultrastructure observation;metabolome;enzymatic activity;molecular docking
摘要：
The aim of the present investigation was to determine the active ingredients in Amaran-thus tricolor L. leaves and develop a biological pesticide. Organic solvent extraction, column chro-matography, liquid chromatography, ODS-C18 reverse elution, Sephadex LH-20 gel filtration, H spectrum, and C spectrum were used to isolate the pure product for an assessment of the agricultural activity and bacteriostatic mechanisms. The results showed that the activity of the crude extract following carbon powder filtration was 1.63-fold that of the non-filtered extract. Further isolation was performed to obtain two pure products, namely, hydroxybenzoic acid (HBA) and ben-zo[b]furan-2-carboxaldehyde (BFC), and their molecular formulas and molecular weights were C7H6O3 and 138.12, and C9H6O2 and 146.12, respectively. Our study is the first to determine that HBA has bacteriostatic activity (MIC 125 μg/mL) and is also the first to isolate BFC from A. tricolor. The ultrastructure observation results showed that HBA caused the bacteria to become shriveled, distorted, and deformed, as well as exhibit uneven surfaces. After HBA treatment, 70 differentially expressed metabolites were detected in the bacteria, of which 9 were downregulated and 61 were upregulated. The differentially expressed metabolites were mainly strigolactones, organic acids and derivatives, fatty acids, benzene and substituted benzene derivatives, amino acids and associated metabolites, and alcohols and amines. Among all of the downregulated differentially expressed metabolites, MEDP1280 was the most critical, as it participates in many physiological and biochemical processes. The enrichment analysis showed that the differentially expressed metabolites mainly participate in tyrosine metabolism, biosynthesis of amino acids, cysteine and methio-nine metabolism, and arginine and proline metabolism. Additionally, HBA was found to disrupt cell membrane permeability and integrity, causing the leakage of substances and apoptosis. The physiological and biochemical test results showed that HBA could increase the pyruvate levels in bacteria but could decrease the activities of respiratory enzymes (malate dehydrogenase (MDH) and NADH oxidase) and antioxidant enzymes (superoxide dismutase (SOD) and glutathione pe-roxidase (GSH-PX)). Inverse molecular docking was used to study the binding between HBA and respiratory and antioxidant enzymes. The results showed that HBA could bind to MDH, NADH oxidase, SOD, and GSH-PX, suggesting that these enzymes may be the effector targets of HBA. Conclusion: The optimal active ingredient in A. tricolor that can inhibit Acidovorax avenae subsp. citrulli was identified as HBA. HBA mainly disrupts the cell membrane, damages the metabolic system, and inhibits respiration and antioxidant enzyme activity to control bacterial growth. These results provide a reference for the further development of biological pesticides. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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柑橘黄龙病菌分泌蛋白04580基因的克隆及原核表达
作者：
邓杰夫;李魏;雷玲;洪艳云;刘双清;...
期刊：
湖南农业大学学报(自然科学版) ,2022年48(3):294-297 ISSN：1007-1032
作者机构：
湖南农业大学植物保护学院,湖南 长沙 410128;植物病虫害生物学与防控湖南省重点实验室,湖南 长沙 410128;[洪艳云; 邓杰夫; 戴良英; 易图永; 雷玲; 李魏; 刘双清] 湖南农业大学植物保护学院<&wdkj&>植物病虫害生物学与防控湖南省重点实验室
关键词：
柑橘黄龙病菌;分泌蛋白;原核表达;蛋白纯化
摘要：
为进一步了解柑橘黄龙病菌亚洲种Clas的致病机理，从Prasad预测的166种分泌蛋白中选取1个在柑橘植株中表达量较高的CLIBASIA–04580基因进行研究。提取感染柑橘黄龙病的柑橘叶片总DNA，经 PCR扩增得到 CLIBASIA–04580基因，切胶回收PCR产物双酶切连接到pET–28a载体上，使用菌落PCR和限制性内切酶双酶切鉴定筛选阳性克隆；转化大肠杆菌 BL21（DE3），使用0.5、1.0、1.5、2.0 mmol/L的异丙基硫代半乳糖苷（IPTG） 诱导重组蛋白表达融合组氨酸标签的CLIBASIA–04580重组蛋白，聚丙烯酰氨凝胶电泳（SDS–PAGE）检测重组蛋白的表达水平，然后使用蛋白纯化镍柱进行纯化。结果表明，携带组氨酸标签的CLIBASIA–04580重组蛋白得到表达，且异丙基硫代半乳糖苷（IPTG）浓度为1.5 mmol/L时相对表达量达到峰值，通过SDS–PAGE凝胶分析，与未经过镍柱纯化的蛋白原液比较可知成功纯化了CLIBASIA–04580重组蛋白。
语种：
中文
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龙牙百合内生拮抗灰葡萄孢菌细菌的筛选与鉴定及发酵条件优化
作者：
郭垂宝;刘双清;马文月;韩军花;曹海佳;...
期刊：
湖南农业大学学报(自然科学版) ,2022年48(2):190-195 ISSN：1007-1032
作者机构：
[郭垂宝; 张小艳; 曹海佳; 韩军花; 马文月; 刘双清] 湖南农业大学植物保护学院;湖南农业大学植物保护学院,,湖南省生物农药与农药制剂加工工程技术研究中心;[廖晓兰] 湖南农业大学植物保护学院<&wdkj&>湖南省生物农药与农药制剂加工工程技术研究中心
关键词：
龙牙百合;龙牙百合灰霉病菌;内生细菌;发酵条件
摘要：
采用平板分离法、平板对峙法从龙牙百合内生菌中筛选获得了具有抑制灰葡萄孢菌活性的98号菌,其抑菌率为74%;对98号菌进行生理生化测定及16S rDNA序列分析,鉴定为唐菖蒲伯克霍尔德菌(Burkholderia gladioli)。通过单因素和正交试验优化该菌株的发酵条件和最适发酵培养基配方,结果表明,pH值为7、培养温度为32 ℃、接种量为发酵液体积的6%、250 mL三角瓶装100 mL牛肉膏蛋白胨液体发酵培养基、180 r/min的摇床培养5 d,其抑菌活性最大;优化后的最适培养基配方为硫酸亚铁0.15 g、玉米粉20 g、牛肉膏15 g,水1 L。
语种：
中文
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计算机辅助药物设计在新农用化合物开发中的应用
作者：
王俊琳;张亚;刘双清;王翀;廖晓兰
期刊：
农药 ,2022年61(4):242-249,270 ISSN：1006-0413
作者机构：
[张亚; 廖晓兰; 王俊琳; 刘双清] 湖南农业大学植物保护学院;[王翀] 湖南农业大学生物科学技术学院
关键词：
农药分子设计;基于受体的药物设计;基于配体的药物设计;药物靶标预测
摘要：
农药创新关系到我国农业可持续发展和生态环境安全，计算机辅助药物设计能够有效帮助农药创新。介绍了药物靶标的选择和预测、分子对接、分子动力学模拟、从头设计、基于药效团的虚拟筛选和定量构效关系模型等计算机辅助药物设计方法在农药创制过程中的应用，探讨了这些计算机辅助药物设计方法在农药创制过程中存在的问题，展望了计算机辅助药物设计在未来新农药开发中的应用前景。
语种：
中文
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基于深度学习的轻量化田间昆虫识别及分类模型
作者：
袁哲明;袁鸿杰;言雨璇;李钎;刘双清;...
期刊：
吉林大学学报(工学版) ,2021年51(03):1131-1139 ISSN：1671-5497
作者机构：
湖南农业大学湖南省农业大数据分析与决策工程技术研究中心;[言雨璇] 湖南农业大学湖南省农村农业信息化工程技术研究中心;湖南农业大学商学院;[刘双清] 湖南农业大学植物保护学院;[谭泗桥; 袁鸿杰; 袁哲明] 湖南农业大学湖南省农业大数据分析与决策工程技术研究中心<&wdkj&>湖南农业大学湖南省农村农业信息化工程技术研究中心
关键词：
模式识别;深度学习;目标检测;昆虫分类;轻量化算法;图像预处理
摘要：
由于田间昆虫环境的复杂性、昆虫类别间样本数量的不均衡性,现有田间昆虫自动识别和分类方法存在误识率高、效率低等缺点。本文基于轻量化深度学习模型提出了新的田间昆虫自动识别和分类算法。首先,对田间昆虫图像进行预处理,将其输入到轻量化算法中进行特征提取,通过多尺度特征融合输出不同尺寸的预测网络。然后,引入联合交并比进行田间昆虫自动识别和分类。最后,与其他算法进行了仿真对比实验,结果表明,本文算法的田间昆虫自动识别和分类正确率高、用时少、鲁棒性强,有效解决了昆虫堆积、背景干扰等问题,可实时、在线识别田间昆虫。
语种：
中文
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湖南省农业科技社会化服务体系建设的现状分析与实现路径
作者：
刘双清;吕军;黄大金
期刊：
科技成果管理与研究 ,2021年16(7):15-19 ISSN：1673-6516
作者机构：
湖南农业大学科学技术处/新农村发展研究院,湖南 长沙 410128;[吕军] 湖南省平江县农业农村局植保植检站,湖南 平江 414500;湖南农业大学经济学院,湖南 长沙 410128;[黄大金; 刘双清] 湖南农业大学
关键词：
湖南省;农业科技社会化服务体系;实现途径
摘要：
当前农业农村发展处于重大转型时期,建设农业科技社会化服务体系是服务支撑乡村振兴战略的重要抓手,是新形势下"三农"工作的重要任务.通过分析湖南省农业科技社会化服务体系建设面临的形势和挑战,从顶层设计、服务体制与机制、服务职能等方面探讨加强湖南省农业科技社会化服务体系建设的发展思路与实现途径.
语种：
中文
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Inhibition molecular mechanism of the novel fungicidal n-(Naphthalen-1-yl) phenazine-1-carboxamide against rhizoctonia solani
作者：
Zhang, Ya;Wang, Chong* ;Liu, Shuangqing* ;Liao, Xiaolan
期刊：
Agronomy ,2021年11(12) ISSN：2073-4395
通讯作者：
Liu, Shuangqing;Wang, Chong
作者机构：
[Zhang, Ya; Liu, Shuangqing; Liao, Xiaolan] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China.;[Wang, Chong] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
通讯机构：
[Liu, Shuangqing; Wang, Chong] H;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
关键词：
Differential genes;Differential pathways;Fluorescence quantification;Key enzyme activity;Molecular binding mode;Novel compounds;Rhizoctonia solani;Transcriptome
摘要：
To explore the molecular mechanism through which the novel fungicide N-(naphthalen-1-yl) phenazine-1-carboxamide (NNPCN) inhibits Rhizoctonia solani, we clarified the target and mode of action, explored lead compounds, and developed novel fungicides. Methods: Growth observa-tion, scanning electron microscopy, transmission electron microscopy, transcriptome sequencing technology, quantitative real-time PCR (qRT-PCR), physiological and biochemical determination, and reverse molecular docking technology were used to study the effects of this compound on the microscopic morphology of R. solani. The differentially expressed genes (DEGs), functions, and metabolic pathways were analyzed. The genes displaying significant differences were randomly selected for qRT-PCR verification and confirmed by physiological and biochemical determination to construct their binding mode with key targets. The results showed that the mycelium treated with NNPCN produced a red secretion and exhibited progressive creeping growth. Under a scanning electron microscope, hyphal swelling, uneven thickness, fractures, deformities, and hyphal surface warts increased. Under a transmission electron microscope, the cell wall was separated, the subcellular organelles were disintegrated, and the septum disappeared. Furthermore, there were 6838 DEGs under NNPCN treatment, including 291 significant DEGs, of which 143 were upregulated and 148 downregulated. Ten DEGs were randomly selected for qRT-PCR verification, and the gene expression trend was consistent with the transcriptome sequencing results. Gene Ontology enrichment analysis showed that the DEGs were significantly enriched in cell wall glucan decomposition and metabolism, cell membrane synthesis, metabolism, composition, organic hydroxyl compounds, oxidoreductase activity, and transition metal ion binding. Metabolic pathway enrichment analysis showed that there were 16 significant metabolic pathways, such as steroid biosynthesis and ABC transporters. Further study found that genes, such as the glycosyl hydrolase family 10 domaincontaining protein, which is related to glucan catabolic process function as tied to the cell wall, were downregulated. Lipid oxidation, modification, and other genes related to the cell membrane were also downregulated. Secondly, genes related to lipid modification, lipid metabolism processes, integral components of the membrane, and other ABC transporters were downregulated. Fatty-acid oxidation and carbohydrate metabolic processes, which are related to antioxidant and metabolic functions, displayed significant differences in their target genes. Nitrite reductase [NADH] activity and mitochondrial organization gene expression were downregulated. These results revealed that target genes may involved in the cell wall, cell membrane, antioxidant and metabolism, nitrogen metabolism, and mitochondria. The results of the physiological and biochemical tests showed that NNPCN decreased the β-1,3-glucanase, malondialdehyde, and ATPase activities and nucleic acid leakage but increased the activity of nitrate reductase. The results of the reverse molecular docking showed that NNPCN could freely bind to target proteins such as β-1,3-glucanase, ABC transporter, and NADPH nitrate reductase, whereby NNPCN could bind to glucanase via van der Waals and electrostatic forces and to ABC transporter and NADPH nitrate reductase via hydrogen bonding. Conclusion: The mechanism via which NNPCN inhibits R. solani may be related to the cell wall structure, cell membrane damage, antioxidant activity, and metabolism. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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湖南省农业科技发展的形势趋势分析与提升对策探讨
作者：
刘双清;吕军;黄大金
期刊：
科技成果管理与研究 ,2021年16(11):25-30 ISSN：1673-6516
作者机构：
[刘双清] 湖南农业大学科学技术处、新农村发展研究院,湖南 长沙 410128;湖南省平江县农业农村局植保植检站,湖南 平江 414500;湖南农业大学经济学院,湖南 长沙 410128;[吕军] 湖南省平江县农业农村局植保植检站;[黄大金] 湖南农业大学
关键词：
农业科技;乡村振兴;农业农村发展
摘要：
农业科技创新是促进农业农村经济增长最重要的驱动力,为保障国家粮食安全,促进农业增效、农民增收和农村发展发挥了重要作用.通过梳理我国农业农村发展的形势与趋势,分析湖南省农业科技发展的现状,探讨推动湖南省农业科技发展的战略与对策.
语种：
中文
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The degradation behaviour, residue distribution, and dietary risk assessment of malathion on vegetables and fruits in China by GC-FPD
作者：
Liu, Yiping;Liu, Shuangqing;Zhang, Ya;Qin, Dongmei;Zheng, Zuntao;...
期刊：
Food Control ,2020年107:106754 ISSN：0956-7135
通讯作者：
Liao, Xiaolan;Li, Xiaogang
作者机构：
[Zhang, Ya; Liu, Shuangqing; Li, Xiaogang; Liao, Xiaolan; Liu, Yiping] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Zheng, Zuntao; Qin, Dongmei; Zhu, Guangyan] Minist Agr & Rural Affairs, Inst Control Agrochem, Beijing 100125, Peoples R China.;[Liu, Zhaoqing; Lv, Yuntao; Liu, Yiping] Hunan Prov Dept Agr & Rural Affairs, Hunan Prov Inst Control Agrochem, Changsha 410005, Hunan, Peoples R China.;[Dong, Zhe] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China.;[Liao, Xiaolan; Li, XG] 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liao, XL; Li, XG] 1;1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.
关键词：
Degradation kinetics;Dietary risk assessment;Gas chromatography;Malathion;Maximum residual limits
摘要：
The gas chromatography-flame photometric detector (GC-FPD) was used to detect malathion residues content in vegetables (cherry tomatoes and broccoli) and fruits (mulberries, cranberries, and figs). The recoveries of samples were 76.2 ± 3.5%–103.9 ± 3.6% at a spike level of 0.01–10 mg/kg, the relative standard deviation (RSDs) were 2.1%–7.3%, and the limit of quantification (LOQs) were 0.01–0.02 mg/kg. On the basis of the normative field trials, the degradation half-life of malathion were 1.10–2.15 days, which indicated that malathion degrades quickly. The final distribution of malathion residues in various vegetables and fruits varied from <0.01 to 0.58 mg/kg. The long-term dietary risk assessment results showed that the national estimated daily intake (NEDI) of malathion in various populations of China ranged from 0.000021 to 0.000549 mg/kg bw, and the risk quotient (RQ) ranged from 0.007% to 0.183% and were less than 100%, which indicated that malathion has a low risk for long-term dietary intake these crops. The maximum residual limits (MRLs) of malathion can be set as 1 mg/kg for cherry tomatoes, broccoli, mulberries, cranberries, and 0.2 mg/kg for figs, respectively. These results would be considered as important references for monitoring and assessing the quality safety of agricultural products and protecting consumer health. © 2019 Elsevier Ltd
语种：
英文
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柑橘主要病害成灾机理及绿色防控关键技术与应用
作者：
易图永;王运生;谭小平;何可佳;戴良英;...
期刊：
中国科技成果 ,2020年(20):11-13 ISSN：1009-5659
作者机构：
湖南农业大学植物保护学院,湖南 长沙 410128;湖南省植保植检站,湖南 长沙 410006;[洪艳云; 易图永; 戴良英; 何可佳; 唐前君; 王运生; 刘双清] 湖南农业大学;[谭小平; 张佳峰] 湖南省植保植检站
摘要：
柑橘是我国重要的水果产业,有4,000多年的栽培历史.目前,我国柑橘种植面积为4,000万亩,产量4,000余万吨,均居世界前列.但柑橘溃疡病、黄龙病为重要的检疫性病害,柑橘砂皮病影响树势和果品外观,造成严重的经济损失.据怀化市植保植检站调查,严重的果园,柑橘砂皮病的病果率在80％以上,病情指数高达63.3,砂皮病果最低以几分钱一斤被处理掉.按砂皮病果比正常果平均价格低1元/kg计算,2013年、2014年、2015年,怀化市柑橘砂皮病果约50万吨、56万吨、85万吨,3年损失分别达5亿元、5.6亿元、8.5亿元.
语种：
中文
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