作者：
Li, F. N.*;Li, L. L.;Yang, H. S.;Yuan, X. X.;Zhang, B.;...
期刊：
Animal,2011年5(12):1903-1908 ISSN：1751-7311
通讯作者：
Li, F. N.
作者机构：
[Yang, H. S.; Li, L. L.; Geng, M. M.; Yin, Y. L.; Li, F. N.; Yuan, X. X.] Chinese Acad Sci, Lab Agroecol Proc Subtrop Reg, Res Ctr Hlth Breeding Livestock & Poultry, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Zhang, B.] Hunan Agr Univ, Coll Anim Sci, Changsha 410128, Hunan, Peoples R China.;[Yang, H. S.; Yuan, X. X.] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China.;[Xiao, C. W.] Hlth Canada, Banting Res Ctr 2203E, Food Directorate, Nutr Res Div, Ottawa, ON K1A 0K9, Canada.
通讯机构：
[Li, F. N.] C;Chinese Acad Sci, Lab Agroecol Proc Subtrop Reg, Res Ctr Hlth Breeding Livestock & Poultry, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.
摘要：
To define the molecular response of Acidithiobacillus ferrooxidans under pH up-shift, temporal gene expression profiles were examined by using whole-genome DNA microarrays for A. ferrooxidans. Approximately 30% of the 3,132 genes represented on the microarray were significantly upregulated over a 160-min period, while about 14% were significantly downregulated. Our results revealed that A. ferrooxidans showed potential self-protection and self-regulation performance in response to pH up-shift stress. Many genes involved in regulation of membrane components were differentially expressed under the pH up-shift stress. Likewise, most of genes involved in phosphate metabolism, sulfur assimilation, and CO2 fixation were obviously induced. Conversely, the transcription of a polyphosphate kinase gene (AFE1210) associated with phosphate storage was significantly repressed, which probably stemmed from the depletion of polyphosphate. Besides, most of the genes involved in hydrogen uptake were significantly induced, whereas many genes involved in nitrogen fixation were obviously repressed, which suggested that hydrogen uptake and nitrogen fixation could contribute to cytoplasmic pH homeostasis.
摘要：
BACKGROUND, AIM, AND SCOPE: Ferro-cyanide is one of the commonly found species at cyanide-contaminated soils and groundwater. Unlike botanical metabolism of KCN via the beta-cyanoalanine pathway, processes involved in the plant-mediated assimilation of ferro-cyanide are still unclear. The objective of this study was to investigate a possible mechanism involved in uptake and assimilation of ferro-cyanide by plants. MATERIALS AND METHODS: Detached roots of plants were exposed to ferro-cyanide in a closed-dark hydroponic system amended with HgCl(2), AgNO(3), LaCl(3), tetraethylammonium chloride (TEACl), or Na(3)VO(4), respectively, at 25 +/- 0.5 degrees C for 24 h. Total CN, free CN(-), and dissolved Fe(2+) were analyzed spectrophotometrically. Activity of beta-cyanoalanine synthase involved in cyanide assimilation was also assayed using detached roots of plants in vivo. RESULTS: Dissociation of ferro-cyanide [Fe(II)(CN)(6)](-4) to free CN(-) and Fe(2+) in solution was negligible. The applied inhibitors did not show any significant impact on the uptake of ferro-cyanide by soybean (Glycine max L. cv. JD 1) and hybrid willows (Salix matsudana Koidz x alba L.; p > 0.05), but rice (Oryza sativa L. cv. JY 98) was more susceptible to the inhibitors compared with the controls (p < 0.05). However, TEACl had the most severe effect on the assimilation of ferro-cyanide by soybean, hybrid willows, and maize (Zea mays L. cv. PA 78; p < 0.01), whereas AgNO(3) was the most sensitive inhibitor to rice (p < 0.01). No measurable difference in beta-cyanoalanine synthase activity of roots exposed to ferro-cyanide was observed compared with the control without any cyanides (p > 0.05), whereas roots exposed to KCN showed a considerable increase in enzyme activity (p < 0.05). CONCLUSIONS: Plants take up Fe(2+) and CN(-) as a whole complex, and in vivo dissociation to free CN(-) is not prerequisite during the botanical assimilation of ferro-cyanide. Ferro-cyanide is likely metabolized by plants directly through an unknown pathway rather than the beta-cyanoalanine pathway.
摘要：
Cold stress is one of the major abiotic stresses limiting the productivity and the geographical distribution of many important crops. To gain a better understanding of cold stress responses in tobacco ( Nicotiana tabacum ), we carried out a comparative proteomic analysis. Five-week-old tobacco seedlings were treated at 4°C for 4 h. Cold treatment resulted in stress phenotypes of smoothing and shallowing leaves and increased relative electrolyte leakage. The expression changes of total proteins in tobacco leaves were examined using two-dimensional electrophoresis. Quantitative image analysis revealed a total of 101 protein spots that changed their intensities significantly, 21 protein spots were down-regulated, eight were up-regulated after the cold treatment, 50 protein spots only expressed in the control sample, while 22 protein spots were only present in the cold treatment sample. Mass spectrometry analysis allowed the identification of 73 differentially expressed proteins, including well known and novel cold-responsive proteins. The identified proteins are involved in several processes such as photosynthesis, protein processing, redox homeostasis, ribonucleic acid (RNA) processing, signal transduction, translation, cell division/cycle, and metabolisms of carbon and energy. Several types of proteins showed enhanced degradation during chilling stress, especially the photosynthetic proteins. Gene expression analysis of 25 different proteins by reverse transcriptase- polymerase chain reaction (RT-PCR) showed that the messenger RNA (mRNA) levels of 18 genes correlated well with the protein levels. In conclusion, our study provides new insights into cold stress responses in tobacco and needs to be further studied in future. Key words : Proteomics, cold stress, tobacco (Nicotiana tabacum).
摘要：
The potential for photo-induced dissociation of ferri- and ferro-cyanide was investigated. The overall reactions followed first order kinetics, judged by the free cyanide analyzed in aqueous solution. The dissociation rates for ferri- and ferro-cyanide were mathematically described by the equations: C
(CN,t) = C
(CN,O)e1.3t
and C
(CN,t) = C
(CN,O)e0.39t
, respectively. In addition, photo-induced dissociation of both iron cyanides was enhanced under an alkaline environment than a neutral condition. Results from the temperature-dependent tests indicated that the dissociation rate of ferri- cyanide was significantly higher than that of ferro-cyanide at all treatment temperatures. The kinetic parameter, activation energy (E
a
) was also experimentally determined to be 12.02 and 12.32 kJ/mol for ferri- and ferro-cyanide, respectively. The results obtained suggest that both iron cyanides are susceptible to photo-dissociation and the rates are positively correlated to the change of temperatures. The information collectively also has important implications for waste management of iron cyanides as well as for risk assessment in a field trial.
摘要：
Forty-eight German Landrace weaned pigs were used in this study to investigate the effects of Lfcin B and Cec P1 on performance, faecal score and DM of weaned piglets orally challenged with enterotoxigenic Escherichia coli F4. The piglets were assigned randomly into four groups: Control, enterotoxigenic Escherichia coli F4ac (ETEC), enterotoxigenic Escherichia coli F4ac (ETEC)+lactoferricin B (Lfcin B) and enterotoxigenic Escherichia coli F4ac (ETEC) +Cecropin P1 (Cec P1) on the basis of weight and litter. All piglets in three experimental groups were orally challenged with 3x109 cfu E.coli K88 O149:K91:F4ac on d 29 and 30. Lfcin B and Cec P1 were administered orally as a single dose of 2 mg per piglet on d 29 after being challenged ETEC F4ac 3 hours later. From the age of 30 days, the piglets were offered feed and water ad libitum. The results showed that there were no significant differences on daily weight, ADG and ADFI (P>0.05) of piglets among the four groups during the experimental period. From d 28 to 35, the piglets in Cec P1 groups kept higher daily weight (p>0.05) than those in other three groups. There were no differences for faecal score and feces dry matter among the four groups. But there was a tendency piglet in Cec P1 group kept the balance of faecal score and feces dry matter. Though there are no obvious effects, Lfcin B and Cec P1 have potential use without toxicity in vivo as additive in the husbandry product.
通讯机构：
[Yu, Xing-Long] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Defective interfering RNA;Porcine reproductive and respiratory syndrome virus
摘要：
In the present study, seven new defective interfering (DI) RNA species of porcine reproductive and respiratory syndrome virus (PRRSV) were identified. RT-PCR, Northern blot and sequence analyses indicated that these DI RNA specie have deletions of 8513-9176 nucleotides located between Nsp1/Nsp2 and Nsp10. Compared with the previous DI RNAs of PRRSV reported, they have three distinct characteristics: much smaller deletion sizes; different nucleotide repeats (2-12 nt) used in the junction sites and in-frame deletions. The results further suggested that the similarity-assisted RNA recombination may be the main cause of generation of DI RNAs in PRRSV and probably in other arteriviruses. (C) 2011 Elsevier B.V. All rights reserved.
作者机构：
[Li, Qingjie] Univ Texas Med Branch Galveston, Div Gastroenterol, Dept Internal Med, Galveston, TX 77550 USA.;[Loehr, Christiane V.] Oregon State Univ, Coll Vet Med, Corvallis, OR 97331 USA.;[Dashwood, Roderick H.] Oregon State Univ, Linus Pauling Inst, Corvallis, OR 97331 USA.;[Dashwood, Roderick H.] Oregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA.;[Luo, Cunhui] Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.
通讯机构：
[Li, Qingjie] U;Univ Texas Med Branch Galveston, Div Gastroenterol, Dept Internal Med, Galveston, TX 77550 USA.
关键词：
activator protein-1;activator protein AP-2;GATA4 transcription factor;liver;protein kinase A
摘要：
Aim: Activator protein 2 alpha (AP-2 alpha) belongs to the AP-2 family of transcription factors that are involved in the regulation of cell proliferation, differentiation, apoptosis and carcinogenesis and has been suggested to function as a tumor suppressor in many cancers. However, the physiological role of AP-2 alpha in hepatocytes is unknown. The present study is to characterize the expression and function of AP-2 alpha in the liver of conscience mouse. Methods: Exogenous AP-2 alpha was overexpressed in the mouse liver by in vivo gene delivery and changes in transcription factor expression were identified by using protein-DNA arrays and immunoblotting. Results: Western blotting and protein/DNA arrays showed that AP-2 alpha is expressed in the nuclei of mouse hepatocytes. Overexpression of AP-2 alpha in vivo significantly suppressed transcription factors AP-1, CREB and c-Myc, and markedly increased CBF, c-Myb, NF-1, Pax-5, RXR, Smad3/4, TR(DR-4), USF-1 and GATA. Among all GATA proteins, only GATA-4 level was dramatically elevated and there was a concomitant loss of phospho-GATA-4. Corresponding changes were detected in upstream kinases Akt, GSK-3 beta and PKA, which regulates the phosphorylation status and stability of GATA-4 protein. Conclusions: AP-2 alpha is expressed in mouse hepatocytes and it acts as a master regulator of numerous transcription factors in the liver.
作者：
Tan, C. Y.;Zhong, R. Z.;Tan, Z. L.*;Han, X. F.;Tang, S. X.;...
期刊：
Lipids,2011年46(3):239-247 ISSN：0024-4201
通讯作者：
Tan, Z. L.
作者机构：
[Zhong, R. Z.; Tang, S. X.; Tan, Z. L.; Han, X. F.; Sun, Z. H.; Tan, C. Y.; Wang, M.] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.;[Zhong, R. Z.; Sun, Z. H.; Tan, C. Y.] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China.;[Xiao, W. J.] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
摘要：
This study was conducted to examine dietary tea catechins (TC) supplementation on the fatty acid composition of muscle and ruminal bacteria in goats fed a maize stover-based diet. Forty goats, 8 months old (16.2 +/- 1.2 kg), were randomly divided into four equal groups (10 animals in each group) and assigned to four experiment diets with TC supplementation at four levels (0, 2,000, 3,000 and 4,000 mg TC/kg feed, namely TC0, TC2000, TC3000 and TC4000, respectively). After a 60-day feeding period, all the goats were slaughtered and sampled. The results showed that dietary TC inclusion increased the average daily gain (ADG), protein content in the semimembranosus muscle and dry matter in the longissimus dorsi muscle (LD). Dietary TC supplementation increased the ratio of n-6 to n-3 fatty acid, the ratio of polyunsaturated fatty acids to saturated fatty acids was higher in TC3000 and TC4000 than in TC0 and TC2000 for LD. The current results implied that dietary inclusion of a suitable TC dose could improve the growth performance and increase the proportions of unsaturated fatty acids in muscle, and the biohydrogenation of ruminal microorganisms might change the profiles of fatty acids in the muscle of growing goats.
摘要：
An allele of the cytochrome P450 gene, CYP6AE14, named CYP6AE25 (GenBank accession no. EU807990) was isolated from the Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Pyralidae) by RT-PCR. The cDNA sequence of CYP6AE25 is 2315 bp in length and contains a 1569 nucleotides open reading frame encoding a putative protein with 523 amino acid residues and a predicted molecular weight of 59.95 kDa and a theoretical pI of 8.31. The putative protein contains the classic heme-binding sequence motif F××G×××C×G (residues 451-460) conserved among all P450 enzymes as well as other characteristic motifs of all cytochrome P450s. It shares 52% identity with the previously published sequence of CYP6AE14 (GenBank accession no. DQ986461) from Helicoverpa armigera. Phylogenetic analysis of amino acid sequences from members of various P450 families indicated that CYP6AE25 has a closer phylogenetic relationship with CYP6AE14 and CYP6B1 that are related to metabolism of plant allelochemicals, CYP6D1 which is related to pyrethroid resistance and has a more distant relationship to CYP302A1 and CYP307A1 which are related to synthesis of the insect molting hormones. The expression level of the gene in the adults and immature stages of O. furnacalis by quantitative real-time PCR revealed that CYP6AE25 was expressed in all life stages investigated. The mRNA expression level in 3rd instar larvae was 12.8- and 2.97-fold higher than those in pupae and adults, respectively. The tissue specific expression level of CYP6AE25 was in the order of midgut, malpighian tube and fatty body from high to low but was absent in ovary and brain. The analysis of the CYP6AE25 gene using bioinformatic software is discussed.
摘要：
Double B-box 1a (DBB1a) belongs to the zinc-finger family proteins in Arabidopsis thaliana. Transcriptional analysis uncovered that the DBB1a gene expression was blue light-dependently regulated, and the transcript level of DBB1a in cry1cry2 was decreased but not in phyAphyB compared to wild type under blue light conditions. Transgenic plants containing pDBB1a:GUS (β-glucuronidase) displayed GUS activity in the vascular system of leaves and petioles. Green fluorescent protein (GFP)-fused DDB1a (DBB1a-GFP) protein was found in the nucleus in transient transformation assays with onion epidermal cells as well as in stable transgenic Arabidopsis plants. To investigate the function of DBB1a, we generated DBB1a over-expressing and under-expressing transgenic Arabidopsis plants. Analysis of hypocotyl growth of these lines indicated that DBB1a promoted hypocotyl elongation under blue light condition. The phenotype of transgenic plants with DBB1a over-expression could be impaired by a gibberellin (GA)-biosynthesis inhibitor. Moreover, the expression analysis of GA metabolic and catabolic genes in DBB1a transgenic lines indicated that the DBB1a suppressed GA2-oxidase1 (GA2ox1) and GA2-oxidase8 (GA2ox8) expression, but induced GA3β-hydroxygenase1 (GA3ox1) and GA20-oxidase1 (GA20ox1) expression under blue light. Taken together, we concluded that DBB1a promotes hypocotyl elongation under blue light condition through an increase in bioactive GA levels in Arabidopsis.
