通讯机构：
[Bai, Lian-Yang] H;Hunan Agr Univ, Pesticide Res Inst, Changsha 410128, Hunan, Peoples R China.
摘要：
The purpose of this study was to develop a sensitive and low-cost optical sensor for screening pymetrozine using unmodified gold nanoparticles (G NPs). The presence of pymetrozine could rapidly induce the aggregation of G NPs, thereby resulting in red to deep blue colour change. The concentration of pymetrozine was determined with a limit of detection lower than 1×10
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M by the naked eye and was further easily monitored by a UV-vis spectrometer. The highest selectivity was achieved in comparison with other 11 pesticides and the possible underlying mechanism was investigated in detail by the simultaneous addition of glutathione and pymetrozine in G NPs solution.
摘要：
The y + LAT1 gene is a member of solute carrier family that encodes a protein, which plays a significant function in transport of neutral and dibasic amino acids. The Tibetan pig is an excellent local variety in China, which is able to tolerate and survive under the adverse conditions. The study cloned the cDNA sequence of Tibetan pig y + LAT1 (GenBank accession #EU390782), and evaluated the tissue distribution and developmental expression of y + LAT1 mRNA and protein in different parts of intestine in Tibetan suckling piglets. The results show that the y + LAT1 cDNA encodes 511 deduced amino acid residues and 12 putative transmembrane domains, which can be widely detected in many organs in Tibetan piglets. Expression patterns were similar in jejunum and ileum, where the mRNA and protein level was decreased before the suckling period and increased until Day 35, duodenum changed reversely. The jejunum posterior is the predominant expressional tissue in all days. These results indicated that the ontogenesis expression of y + LAT1 was not only differentially regulated by early development age but also differentially with distributed tissues along the small intestine.
期刊：
JOURNAL OF OPTOELECTRONICS AND ADVANCED MATERIALS,2010年12(6):1289-1296 ISSN：1454-4164
通讯作者：
Liang, Y.
作者机构：
[Ouyang, Y.; Liang, Y.] Cent S Univ, Res Ctr Modernizat Chinese Med, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Tang, Z.] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liang, Y.] C;Cent S Univ, Res Ctr Modernizat Chinese Med, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
关键词：
Potential high energy density materials (HEDMs);Polynitrotriprismanes (PNNPs);Heats of formation (HOFs);Strain energies (SE);Disproportionation energy (DE);Bond dissociation energy (BDE)
摘要：
To further test whether polynitriprismanes can be capable of being potential high energy density materials (HEDMs), extensive theoretical calculations have been carried out to investigate on a series of polynitrotriprismanes (PNNPs): C6H6-n, (NO2)(n)(n=1 similar to 6). Heats of formation (HOFs), strain energies (SE), and disproportionation energy (DE) are obtained using B3LYP/6-311+G (2df, 2p)//B3LYP /6-31G* method by designing different isodesmic reactions, respectively. Detonation properties of PNNPs are obtained by the well-known Kamlet-Jacobs equations, using the predicted densities (p) obtained by Monte Carlo method and HOFs. It is found that they increase as the number of nitro groups n varies from 1 to 6, and PNNPs with have excellent detonation properties. The relative stability and the pyrolysis mechanism of PNNPs are evaluated by calculated bond dissociation energy (BDE). The comparison of BDE suggests that rupturing the C-C bond is the trigger for thermolysis of PNNPs. The computed BDE for cleavage of C-C bond (21.08kcal.mol(-1)) further demonstrates that only the hexanitrotriprismane can be considered to be the target of HEDMs.
摘要：
In this study, we used native gradient-polyacrylamide gel electrophoresis and electroelution (NGGEE) to purify enzymatic proteins from Trichoderma koningii AS3.2774. With this method, we purified eight enzymatic proteins and classified them to the cellulase system by comparing secretions of T. koningii in inductive medium and in repressive medium. It resulted in 24-fold beta-glucosidase (BG) purification with a recovery rate of 5.5%, and a specific activity of 994.6 IU mg(-1) protein. The final yield of BG reached 8 mu g under purifying procedure of NGGEE We also identified BG using the enzyme assay with thin-layer chromatography and MALDI-TOFMS. This BG had one subunit with a molecular mass of 69.1 kDa as determined by sodium dodecylsulfate-polyacrylamide gel electrophoresis. The hydrolytic activity of the BG had an optimal pH of 5.0, an optimal temperature of 50 degrees C. an isoelectric point of 5.68 and a K-m for p-nitrophenyl-beta-D-glucopyranoside of 2.67 mM. Taken together, we show that NGGEE is a reliable method through which mu g grade of active proteins can be purified. (C) 2010 Elsevier B.V. All rights reserved.
摘要：
The ZnO nanorods with different diameters (similar to 300, 100, 40 nm) and nanoparticles (similar to 6 nm) were prepared, and their sensitivities to formaldehyde at room temperature were measured with and without the UV light irradiation (the intensity of light is 0.155 mW/cm(2)). The XRD, TEM and SEM were utilized to examine the size of crystal and the morphology of material. The transient photovoltage and photoluminescence spectra were utilized to investigate the photoelectric properties of ZnO. The ZnO nanorods with diameter of similar to 40 nm own the highest response to formaldehyde with the UV light illumination than that of other samples. It is attributed to the large surface-to-volume ratio and high photo-generated charge efficiency, which are two key parameters to determine the gas response. The transient photovoltage results further demonstrate that the photo-generated charge efficiency of gas sensing element decreases as the surface-to-volume ratio increases (i.e. the size of crystal decreases). Therefore, the particles with highest sensitivity are of a certain size, neither the largest nor the smallest tested. Our work may supply a direction to fabricate the high performance gas sensor with UV light illumination. (C) 2010 Elsevier B.V. All rights reserved.
作者：
Hu, Q.;Liu, Z. Q.;Yao, K.;Wu, X.;Yang, L. Y.;...
期刊：
Journal of Food, Agriculture and Environment,2010年8(3-4):39-46 ISSN：1459-0255
通讯作者：
Ruan, Z.
作者机构：
[Deng, Z. Y.; Ruan, Z.; Hu, Q.] Nanchang Univ, Coll Life Sci & Food Engn, State Key Lab Food Sci & Technol, Nanchang 330031, Peoples R China.;[Liu, Z. Q.; Wu, X.; Hu, Q.; Pan, J.; Yao, K.] Chinese Acad Sci, Inst Subtrop Agr, Lab Anim Nutr & Hlth, Changsha 410125, Hunan, Peoples R China.;[Yang, L. Y.] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.
通讯机构：
[Ruan, Z.] N;Nanchang Univ, Coll Life Sci & Food Engn, State Key Lab Food Sci & Technol, Nanchang 330031, Peoples R China.
关键词：
Developmental pattern;suckled crossbred piglets;gene expression;growth hormone;insulin-like growth factor I
摘要：
A total of thirty (15 male and female, respectively) suckled crossbred piglets (DurocxLandraccxYorkshine) were selected and divided into five groups at the ages of 1, 7, 14, 21 and 28 d after birth, respectively. Piglets were weighed and blood and tissue (liver and longissimus dorsi) samples were collected from a total of 30 piglets at different ages. There were no significant differences (P>0.05) between male and female, but there were significant differences (P<0.05) among ages during the early postnatal development for the concentration and mRNA abundance in serum, liver and longissimus dorsi (LD) of suckled crossbred piglets growth hormone and insulin-like growth factor-I (IGF-I). There were the different development curve pattern (linear, quadratic, cubic or quartic) of age effects (P<0.05) on the concentration and mRNA expression of suckled crossbred piglets growth hormone GH and IGF-I during the early postnatal development from 1 to 28 d of ages. Pearson's analysis showed serum OH concentration correlated with serum IGF-I concentration, IGF-I mRNA abundance of LD, BW and the relative body weight of LD. Serum IGF-I concentration correlated with liver IGF-I concentration, IGF-I mRNA abundance of LD and BW. Liver IGF-I concentration correlated with GHR mRNA abundance of LD, IGF-I mRNA abundance of liver and LD, IGF-IR mRNA abundance of LD, BW and the relative body weight of LD. LD IGF-I concentration correlated with IGF-I mRNA abundance of liver and LD, IGF-IR mRNA abundance of LD and BW. Liver IGF-I mRNA abundance correlated with growth hormone receptor GHR mRNA abundance of liver, IGF-I mRNA abundance of LD, BW and the relative body weight of LD. LD IGF-I mRNA abundance correlated with GHR mRNA abundance of liver, IGF-IR mRNA abundance of LD, BW and the relative body weight of LD, respectively.
摘要：
The present study describes a rapid and sensitive HPLC method for the quantification of huperzine A (HupA) in Huperzia crispata (Huperziaceae). The sample extraction and preparation involved a simple, time-saving, single-solvent extraction, with each sample being analyzed within 12 min. The mobile phase was ammonium acetate (0.1 M, pH 6.0)-methanol (64 + 36, v/v) at a flow rate of 1.0 mL/min. Detection was at 308 nm. The calibration curve was linear from 0.049 to 7.84 μg (R<sup>2</sup> = 0.9997), with intraday and interday precision RSD of less than 2%. The extraction recovery rate was over 98.49%. Quantification of HupA was performed using this modified method, and the content of HupA was 1.86 times higher in the whole plant of H. crispata (218.17 ±1.55 μg/g) than in that of H. serrata (117.03 ±2.97 μg/g). In the whole plant of H. crispata, HupA mainly accumulated in the actively growing shoot tips, the apical bud, and the 10 youngest leaves, reaching 455.23 ±2.97 μg/g. The content of HupA in the samples from sunshine-sheltered sites was 3.45 times higher than in that from sunshine-abundant sites. The satisfactory results indicate that this modified method can be applied in the quality control of large-scale Huperziaceae plant extracts and that changes should be made in the cultivation of H. crispata so as to maximize the production of HupA.
摘要：
We studied the allelopathic effects of Mikania micrantha on seed vigour, seed germination and seedling growth of three receptor species (Robinia pseudoacacia L., Lolium perenne L. and Lagerstroemia indica L) under different exposure times. The target species were selected from the Mikania-'mvaded ecosystem in South China. The inhibitory effects on seed vigour, seed germination and seedling growth of these test species increased with increasing exposure times and at higher extract concentrations. Seed vigour was more sensitive to extracts of Mikania than seed germination and seedling growth. Robinia pseudoacacia was more resistant to Mikania extract than Lolium perenne and Lagerstroemia indica. We found that even short exposure time (50-100 h) to Mikania extract had strong allelopathic effects on seed vigour, seed germination and seedling growth of test species.
作者机构：
[Zeng, D. L.; Liu, W.; Wang, K. Z.; Liu, Y.] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Zhu, X. Q.] CAAS, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China.;[Zhao, G. H.] NW A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi Prov, Peoples R China.;[Tan, M. Y.] Ctr Qual Quarantine Livestock Poultry & Aquacultu, Changsha 410006, Hunan, Peoples R China.;[Zhao, G. H.; Lin, R. Q.; Zhu, X. Q.; Yuan, Z. G.] S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China.
通讯机构：
[Zhu, X. Q.] C;CAAS, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China.
摘要：
The prevalence of spargana infection in frogs (Rana nigromaculata) was investigated in China's central Hunan Province, from March 2007 to October 2009. 59 of 292 (20.2%) wild-caught frogs were found to be infected with plerocercoids (spargana) of the genus Spirometra. Spargana were recovered from the skeletal muscle of the hind limb. The infection rate ranged from 4.5% to 27.4%, and the infection intensity was 1-15 spargana per frog. To identify the species identity of the collected spargana, a portion of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was amplified, sequenced, and analyzed. Sequence variations for cox1 among all the examined spargana were 0.0-3.1%, with 14 variable sites being identified in sequences obtained (3.1%, 14/446), representing 6 different cox1 sequences. Phylogenetic analysis showed that all the spargana isolates in Hunan province represented Spirometra erinaceieuropaei. This is the first report of S. erinaceieuropaei infection in frogs in Hunan province, China. (C) 2010 Elsevier B.V. All rights reserved.
摘要：
With environmental and food safety issues becoming one of the greatest concerns of the public, active plant compounds play more and more important roles in pest controlling. Fern are of great significance for application as new pesticides due to their particular status in plant taxology and co-evolution with insects. In this study, methanol extracts were distilled from wild fern and gymnosperm of 54 species of 22 families collected from Shiwandashan Mountain in Guangxi Zhuang Autonomous Region of China. Insecticidal activities of the methanol extracts were assayed on both adult house fly (Musca domestica) and mosquito (Aedes albopictus). Significant insecticidal activities were exhibited in the methanol extracts of five species, Cupressus funebris (leaves and stems), Cycas acuminatissima (roots), Keteleeria fortunei (leaves and stems), Onychium japonicum (whole plant), and Pinus taiwanensis var. Daming shanensis (leaves and stems). Applications of the extracts of these plants resulted in higher than 50% mortalities in 4th instar larvae of A. albopictus at 24 h after treatment. The methanol extracts from 13 species possessed insecticidal activities against the adult of M. domestica at 48 h after treatment with higher than 90% mortalities. The extensive screening results showed that these fern and gymnosperm were highly potential to be botanical insecticides. The findings provide a feasible and valuable basis for further investigation.
摘要：
Transmissible proventriculitis associated with infectious bronchitis virus (IBV) was at first seen in eastern China in mid-1995, and is now endemic in China. Herein, the complete genome sequence of a proventiculitis-associated infectious bronchitis coronavirus (ZJ971) was sequenced and analyzed. Compared with the genome of the vaccine strain H120, ZJ971 had 54 nucleotide substitutions and a deletion in the 3'-UTR. The substitutions were in the regions of nsp2-nsp5, nsp7, nsp12, nsp13, nsp15, S and N genes, and the untranslating region. The results indicated that ZJ971 could be a variant of IBV strain H120.
作者机构：
[Zhu, X. Z.; Tian, L. X.; Liu, Y. -J.] Sun Yat Sen Univ, Inst Aquat Econ Anim, Sch Life Sci, W Guangzhou, Peoples R China.;[Mai, K. S.] Ocean Univ China, Coll Fisheries, Qingdao, Peoples R China.;[Zhang, Q. H.; Zheng, C. Q.; Cai, M. C.; Zheng, S. X.] Guangdong Yuehai Feed Grp Co Ltd, Zhanjiang, Peoples R China.;[Pan, Q. J.] So Med Univ, Sch Basic Med Sci, Guangzhou, Guangdong, Peoples R China.;[Hu, Y.] Hu Nan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.
通讯机构：
[Liu, Y. -J.] S;Sun Yat Sen Univ, Inst Aquat Econ Anim, Sch Life Sci, 135 Xingang W, Guangzhou 510275, Guangdong, Peoples R China.
摘要：
The objective of this study was to explore the immunomodulatory effects of betulinic acid (BA) extracted from the bark of white birch on mice. Female mice were orally administered BA for 14 days in doses of 0, 0.25, 0.5, and 1 mg/kg body weight. We found that BA significantly enhanced the thymus and spleen indices, and stimulated lymphocyte proliferation induced by Concanavalin A and lipopolysaccharide as shown by MTT assay. Flow cytometry revealed that BA increased the percentage of CD4(+) cells in thymus as well as the percentage of CD19(+) and the ratios of CD4(+)/CD8(+) in spleen. BA increased the number of plaque-forming cell and macrophage phagocytic activity as indicated by a neutral red dye uptake assay, and the peritoneal macrophages levels of TNF-alpha were also increased. In contrast, serum levels of IgG and IgM and serum concentrations of IL-2 and IL-6 were significantly decreased in BA-treated mice compared to the control as assayed by haemagglutination tests and ELISA, respectively. Taken together, these results suggest that BA enhances mouse cellular immunity, humoral immunity, and activity of macrophages. Thus, BA is a potential immune stimulator and may strengthen the immune response of its host.
作者机构：
[Chen, Lusheng] Shihezi Univ, Dept Forestry, Coll Agr, Shihezi 832000, Xinjiang, Peoples R China.;[Wang, Min] S China Agr Univ, Dept Entomol, Guangzhou 510642, Guangdong, Peoples R China.;[Huang, Guohua] Hunan Agr Univ, Inst Entomol, Coll Biosafety Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Wang, Min] S;S China Agr Univ, Dept Entomol, Guangzhou 510642, Guangdong, Peoples R China.
关键词：
Lepidoptera;Notodontidae;Ptilophora;new species
摘要：
The genus Ptilophora Stephens in China is briefly reviewed, with the description of Ptilophora nanlingensissp. n. The new species is most similar to Ptilophora horieaurea in wing pattern and to Ptilophora jezoensis in male genitalia, but they can be distinguished from each other by the following characters: forewing bright reddish brown in Ptilophora nanlingensis, chestnut brown in Ptilophora horieaurea; costa of male genitalia pointed, with a rounded subapical process ventrally in Ptilophora jezoensis, costa rounded, with apex inflated, and with pointed subapical process ventrally in Ptilophora nanlingensis. A key to the Ptilophora species from China and adjacent areas is presented and a distribution map is given. The holotype of the new species is deposited in the Department of Entomology, South China Agricultural University, P. R. China.
